Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,149,544 Δ1 bp 5.4% coding (786/954 nt) fabH → 3‑oxoacyl‑[acyl‑carrier‑protein] synthase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,149,5440G. 5.4% 254.5 / 6.8 74coding (786/954 nt)fabH3‑oxoacyl‑[acyl‑carrier‑protein] synthase III
Reads supporting (aligned to +/- strand):  ref base G (31/39);  new base . (2/2);  total (33/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

CAAGGTTGCGGTAACGGAACTGGCGCACATCGTTGATGAGACGCTGGCGGCGAATAATCTTGACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCT  >  NC_000913/1149397‑1149692
                                                                                                                                                   |                                                                                                                                                    
cAAGGTTGCGGTAACGGAACTGGCGCACATCGTTGATGAGACGCTGGCGGCGAATAATCTTGACCGTTCTCAACTGGACTGGCTGGGTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATg                                                                                                                                                      <  8:6085/148‑1 (MQ=255)
 aaGGTTGCGGTAACGGAACTGGCGCACATCGTTGATGAGACGCTGGCGGCGAATAATCTTGACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATgg                                                                                                                                                     <  3:321822/148‑1 (MQ=255)
 aaGGTTGCGGTAACGGAACTGGCGCACATCGTTGATGAGACGCTGGCGGCGAATAATCTTGACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGa                                                                                                                                                    >  8:34459/1‑149 (MQ=255)
       gCGGTAACGGAACTGGCGCACATCGTTGATGAGACGCTGGCGGCGAATAATCTTGACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATg                                                                                                                                               <  2:205975/148‑1 (MQ=255)
         ggTAACGGAACTGGCGCACATCGTTGATGAGACGCTGGCGGCGAATAATCTTGACCGTGCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCg                                                                                                                                            <  2:144655/149‑1 (MQ=255)
                gAACTGGCGCACATCGTTGATGAGACGCTGGCGGCGAATAATCTTGACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGAc                                                                                                                                     <  2:388179/149‑1 (MQ=255)
                    tGGCGCACATCGTTGATGAGACGCTGGCGGCGAATAATCTTGACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTg                                                                                                                                 >  5:128426/1‑149 (MQ=255)
                         cacaTCGTTGATGAGACGCTGGCGGCGAATAATCTTGACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCg                                                                                                                            <  5:201443/149‑1 (MQ=255)
                              cGTTGATGAGACGCTGGCGGCGAATAATCTTGACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACg                                                                                                                       <  5:176828/149‑1 (MQ=255)
                                  gatgaGACGCTGGCGGCGAATAATCTTGACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATc                                                                                                                             <  4:313414/139‑1 (MQ=255)
                                  gatgaGACGCTGGCGGCGAATAATCTTGACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATc                                                                                                                             >  3:313414/1‑139 (MQ=255)
                                    tgaGACGCTGGCGGCGAATAATCTTGACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATa                                                                                                                 <  6:128426/149‑1 (MQ=255)
                                    tgaGACGCTGGCGGCGAATAATCTTGACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATa                                                                                                                 <  6:135331/149‑1 (MQ=255)
                                    tgaGACGCTGGCGGCGAATAATCTTGACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACGCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATa                                                                                                                 >  8:41038/1‑149 (MQ=255)
                                      agaCGCTGGCGGCGAATAATCTTGACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATAc                                                                                                                <  2:249353/148‑1 (MQ=255)
                                       gaCGCTGGCGGCGAATAATCTTGACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATa                                                                                                                 >  2:165953/1‑146 (MQ=255)
                                       gaCGCTGGCGGCGAATAATCTTGACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATa                                                                                                                 <  1:165953/146‑1 (MQ=255)
                                        aCGCTGGCGGCGAATAATCTTGACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACctc                                                                                                             >  2:51181/1‑149 (MQ=255)
                                              gcggcgAATAATCTTGACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCg                                                                                                         <  8:142794/147‑1 (MQ=255)
                                                 gcgAATAATCTTGACCGTTCTCAACTGGACTGGCTGGTTCCGCATTAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCctc                                                                                                    <  7:61110/149‑1 (MQ=255)
                                                     ataatCTTGACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCGCTGTc                                                                                                <  5:365260/149‑1 (MQ=255)
                                                             gACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATAc                                                                                                                >  7:375294/1‑125 (MQ=255)
                                                             gACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATAc                                                                                                                <  8:375294/125‑1 (MQ=255)
                                                             gACCATTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACTCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTgc                                                                                          <  5:214270/147‑1 (MQ=255)
                                                                cGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCt                                                                                     >  8:409659/1‑149 (MQ=255)
                                                                    ctcAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCgg                                                                                                        <  1:60324/126‑1 (MQ=255)
                                                                    ctcAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCgg                                                                                                        >  2:60324/1‑126 (MQ=255)
                                                                     tcAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATg                                                                                >  4:45591/1‑148 (MQ=255)
                                                                     tcAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATg                                                                                >  4:40516/1‑148 (MQ=255)
                                                                       aaCTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTg                                                                                                  <  3:355045/129‑1 (MQ=255)
                                                                       aaCTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTg                                                                                                  >  4:355045/1‑129 (MQ=255)
                                                                       aaCTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGaa                                                                              <  2:249249/149‑1 (MQ=255)
                                                                       aaCTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGa                                                                               <  7:409659/148‑1 (MQ=255)
                                                                        actggactggCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAg                                                                             <  8:129616/149‑1 (MQ=255)
                                                                        actggactggCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAg                                                                             <  7:197708/149‑1 (MQ=255)
                                                                           ggactggCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTg                                                                          >  8:33579/1‑149 (MQ=255)
                                                                            gactggCTGGTGCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCt                                                                           <  1:51181/147‑1 (MQ=255)
                                                                               tggctggTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGCCGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTAcg                                                                      >  3:254941/1‑149 (MQ=255)
                                                                               tggctggTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTAcg                                                                      >  4:323930/1‑149 (MQ=255)
                                                                                 gctggTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTAc                                                                       <  7:404529/146‑1 (MQ=255)
                                                                                  ctggTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGa                                                                   >  2:81648/1‑149 (MQ=255)
                                                                                   tggTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGAc                                                                  >  8:100892/1‑149 (MQ=255)
                                                                                    ggTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTAcg                                                                      >  8:237479/1‑144 (MQ=255)
                                                                                    ggTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTAcg                                                                      <  7:237479/144‑1 (MQ=255)
                                                                                         cGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACgg                                                                <  7:124306/145‑1 (MQ=255)
                                                                                         cGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACgg                                                                >  8:124306/1‑145 (MQ=255)
                                                                                         cGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGgcgc                                                            <  3:40516/148‑1 (MQ=255)
                                                                                         cGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGACCCGTGCGCGCTGGATGAAGCTGTACGCGACGGgcgc                                                            <  3:45591/148‑1 (MQ=255)
                                                                                                     aCCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCgggg                                                <  7:34459/149‑1 (MQ=255)
                                                                                                     aCCTGCGGATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGGGGTGACGCTGGAGCGCCACTGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCgg                                                  <  7:357871/147‑1 (MQ=255)
                                                                                                      ccTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGc                                               <  7:33579/149‑1 (MQ=255)
                                                                                                       cTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCgggg                                                >  1:125461/1‑147 (MQ=255)
                                                                                                       cTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCgggg                                                >  8:269753/1‑147 (MQ=255)
                                                                                                       cTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGAGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCgggg                                                >  1:62023/1‑147 (MQ=255)
                                                                                                           gTATTTTCAGTGCTACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGtt                                           <  3:322140/148‑1 (MQ=255)
                                                                                                            tattatCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTgg                                         >  4:174583/1‑149 (MQ=255)
                                                                                                            tattatCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTgg                                         <  5:362524/149‑1 (MQ=255)
                                                                                                                atCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCt                                     >  2:159012/1‑149 (MQ=255)
                                                                                                                atCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCt                                     >  8:72947/1‑149 (MQ=255)
                                                                                                                 tCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTg                                    >  2:279826/1‑149 (MQ=255)
                                                                                                                    gTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTaa                                                                                                                   <  2:308192/67‑1 (MQ=255)
                                                                                                                    gTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTaa                                                                                                                   >  1:308192/1‑67 (MQ=255)
                                                                                                                    gTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCtt                                 >  1:302498/1‑149 (MQ=255)
                                                                                                                              gAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGc                                   >  2:315366/1‑137 (MQ=255)
                                                                                                                              gAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTg                       >  4:323036/1‑149 (MQ=255)
                                                                                                                              gAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTg                       <  1:81648/149‑1 (MQ=255)
                                                                                                                                aaaaaCTCGGTATGTCTATGGATAATGTCGTGGTGACTCTCGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGc                     >  7:397943/1‑149 (MQ=255)
                                                                                                                                   aaCTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGt                  <  3:323930/149‑1 (MQ=255)
                                                                                                                                   aaCTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGt                  >  3:106959/1‑149 (MQ=255)
                                                                                                                                    aCTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTg                 <  1:159012/149‑1 (MQ=255)
                                                                                                                                        gttATGTCTATGGATAATGTCGTGGTGCCGCTTGAGAGCCACGGTAATAAGTCTGTGGCCTCTGTCCCGTGCGAGCTGGATGAAGCTGTACGAGACGGGGGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGGGGa               <  6:217899/145‑1 (MQ=255)
                                                                                                                                        ggTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCt                          >  4:293543/1‑136 (MQ=255)
                                                                                                                                        ggTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCt                          <  3:293543/136‑1 (MQ=255)
                                                                                                                                           aTGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCAc          <  4:254941/149‑1 (MQ=255)
                                                                                                                                            tGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGGCCCGTGCGCGCTGGAGGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCAcc         <  1:266573/149‑1 (MQ=255)
                                                                                                                                                  tGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGc   <  4:222332/149‑1 (MQ=255)
                                                                                                                                                   ggATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCt  >  6:46691/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CAAGGTTGCGGTAACGGAACTGGCGCACATCGTTGATGAGACGCTGGCGGCGAATAATCTTGACCGTTCTCAACTGGACTGGCTGGTTCCGCATCAGGCTAACCTGCGTATTATCAGTGCAACGGCGAAAAAACTCGGTATGTCTATGGATAATGTCGTGGTGACGCTGGATCGCCACGGTAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCT  >  NC_000913/1149397‑1149692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: