Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,175,146 Δ1 bp 100% intergenic (‑120/‑242) coaA ← / → thrU pantothenate kinase/tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,175,1460A.99.3% 613.0 / ‑2.0 135intergenic (‑120/‑242)coaA/thrUpantothenate kinase/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base . (67/67);  total (68/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 138 total aligned reads displayed.

TCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTT  >  NC_000913/4174999‑4175290
                                                                                                                                                   |                                                                                                                                                
tCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGa                                                                                                                                                  >  6:537451/1‑148 (MQ=255)
 cATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGaa                                                                                                                                                 >  2:357063/1‑148 (MQ=255)
     aaCGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGaaaaat                                                                                                                                             <  4:515758/148‑2 (MQ=255)
        gTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATctct                                                                                                                                        <  4:79562/149‑1 (MQ=255)
           tGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTATGTTGAATGGGTTAATGGGCAGGAGGGTAACACAGATGGGGAGGAATAATAAGAAAAATCTCTGc                                                                                                                                      >  5:181990/1‑147 (MQ=255)
             ctctTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAg                                                                                                                                   >  8:394730/1‑149 (MQ=255)
             ctctTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCAAg                                                                                                                                   <  2:133778/149‑1 (MQ=255)
               ctTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAgg                                                                                                                                  >  1:163383/1‑148 (MQ=255)
                  ttATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGc                                                                                                                              >  7:291167/1‑149 (MQ=255)
                       cTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCg                                                                                                                         >  7:406196/1‑149 (MQ=255)
                        tCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGt                                                                                                                        >  3:471812/1‑149 (MQ=255)
                          aTAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATctct                                                                                                                                        >  1:140607/1‑131 (MQ=255)
                          aTAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATctct                                                                                                                                        <  2:140607/131‑1 (MQ=255)
                           tAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTGTGTTGAATGGGTGAATGGGCAGGAGGGTAACTCCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTg                                                                                                                      >  1:266515/1‑148 (MQ=255)
                              gCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGaaaa                                                                                                                  <  3:273343/149‑1 (MQ=255)
                                aTGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGc                                                                                                                <  5:209450/149‑1 (MQ=255)
                                   tttCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAt                                                                                                             <  5:537451/149‑1 (MQ=255)
                                     tCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGAGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAtg                                                                                                            <  1:224654/148‑1 (MQ=255)
                                         gCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACa                                                                                                       <  5:292994/149‑1 (MQ=255)
                                             tCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGata                                                                                                   <  6:353580/149‑1 (MQ=255)
                                               tctgCTCTATCGGCAAAGCGATATTTGCCATGGCGATTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTTACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGACAGGAAGCTATCGTTGAAAAGCAATGTGACAGata                                                                                                   <  2:60080/144‑1 (MQ=255)
                                               aTAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGATGCTATCGTTGAAAAGCAATGTGACAGATAtt                                                                                                 >  1:390739/1‑149 (MQ=255)
                                               aTAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGatat                                                                                                  >  1:382971/1‑148 (MQ=255)
                                               aTAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATAtt                                                                                                 >  2:387767/1‑149 (MQ=255)
                                                tAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTc                                                                                                >  5:323687/1‑149 (MQ=255)
                                                 aGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTc                                                                                                >  6:426400/1‑148 (MQ=255)
                                                   ctctATCGGCAAAGCGATATTTGCCAAGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACAAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATCTGACAGata                                                                                                   >  1:60080/1‑143 (MQ=255)
                                                      tATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCt                                                                                           >  2:223022/1‑148 (MQ=255)
                                                      tATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCt                                                                                           >  7:364523/1‑148 (MQ=255)
                                                        tCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGa                                                                                         <  1:387767/148‑1 (MQ=255)
                                                        tCGGCAAAGCGATATTTGCCATGGCGCGGCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACTCCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAATCAATGTGACAGATATTCCATCt                                                                                           <  6:198212/146‑1 (MQ=255)
                                                         cGGCAACGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTGAATGGGCAGGAGGGTAACCCCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGAt                                                                                        >  7:266529/1‑148 (MQ=255)
                                                           gCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAt                                                                                                             <  5:454553/125‑1 (MQ=255)
                                                           gCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAt                                                                                                             >  6:454553/1‑125 (MQ=255)
                                                              aaGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATaaa                                                                                     >  7:161038/1‑146 (MQ=255)
                                                              aaGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATaaa                                                                                     >  3:30194/1‑146 (MQ=255)
                                                                   atatTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTa                                                                             >  7:167781/1‑149 (MQ=255)
                                                                    tatTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTAt                                                                            <  2:41852/149‑1 (MQ=255)
                                                                    tatTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTAt                                                                            <  4:226211/149‑1 (MQ=255)
                                                                    tatTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCAGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTAt                                                                            <  2:390739/149‑1 (MQ=255)
                                                                         gCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGtt                                                                        <  4:30194/148‑1 (MQ=255)
                                                                          ccATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGttt                                                                       <  8:406196/148‑1 (MQ=255)
                                                                          ccATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGtt                                                                        >  7:235327/1‑147 (MQ=255)
                                                                            aTGGCGCTTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCAt                                                                                             <  5:467904/124‑1 (MQ=255)
                                                                            aTGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCAt                                                                                             >  6:467904/1‑124 (MQ=255)
                                                                             tGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCa                                                                   <  1:451902/149‑1 (MQ=255)
                                                                             tGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCa                                                                   <  8:204474/149‑1 (MQ=255)
                                                                               gcgcGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTa                                                                             >  2:60274/1‑137 (MQ=255)
                                                                                  cgTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAgc                                                              <  8:364523/149‑1 (MQ=255)
                                                                                   gTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAgcg                                                             >  4:307763/1‑149 (MQ=255)
                                                                                    tCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAgcg                                                             >  7:486499/1‑149 (MQ=255)
                                                                                    tCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAgcg                                                             >  8:124860/1‑148 (MQ=255)
                                                                                         gTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAAt                                                                               <  6:271908/125‑1 (MQ=255)
                                                                                          ttCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCAttgtt                                                      >  4:496113/1‑149 (MQ=255)
                                                                                          ttCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCAttgtt                                                      <  2:163383/149‑1 (MQ=255)
                                                                                           tCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCAttgtt                                                      >  6:264198/1‑148 (MQ=255)
                                                                                             tGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCAt                                                                                             >  2:454503/1‑107 (MQ=255)
                                                                                             tGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGtt                                                                        <  2:255675/128‑1 (MQ=255)
                                                                                                  aaTGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTa                                                                      <  7:48726/125‑1 (MQ=255)
                                                                                                   aTGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACaa                                              <  3:307763/148‑1 (MQ=255)
                                                                                                    tGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAatg                                            <  7:394730/149‑1 (MQ=255)
                                                                                                    tGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAat                                             >  5:414701/1‑148 (MQ=255)
                                                                                                       gTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAatgatg                                         <  1:357063/149‑1 (MQ=255)
                                                                                                        ttAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGt                                        >  4:387991/1‑149 (MQ=255)
                                                                                                          aaTGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGtt                                       <  8:125459/148‑1 (MQ=255)
                                                                                                          aaTGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGtt                                       >  3:88325/1‑148 (MQ=255)
                                                                                                               gCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGaaa                                   >  5:327203/1‑147 (MQ=255)
                                                                                                                  ggaggGTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGtt                                                                        >  8:63499/1‑107 (MQ=255)
                                                                                                                     gggTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTTCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGc                           <  1:384038/149‑1 (MQ=255)
                                                                                                                      ggTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATc                                                                                            >  8:26703/1‑83 (MQ=255)
                                                                                                                      ggTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATc                                                                                            <  7:26703/83‑1 (MQ=255)
                                                                                                                      ggTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGc                           >  3:501713/1‑148 (MQ=255)
                                                                                                                      ggTAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGc                           >  8:219791/1‑148 (MQ=255)
                                                                                                                        tAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGTAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTaa                        >  6:513066/1‑149 (MQ=255)
                                                                                                                        tAACACCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTaa                        <  4:385938/149‑1 (MQ=255)
                                                                                                                          acacCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAAt                       <  8:266529/148‑1 (MQ=255)
                                                                                                                          acacCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAAt                       <  8:235327/148‑1 (MQ=255)
                                                                                                                            acCAGATGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTg                    >  2:47315/1‑149 (MQ=255)
                                                                                                                                  tGGGGAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTcc              >  8:323240/1‑149 (MQ=255)
                                                                                                                                    gggAGGAATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCg             <  1:223022/148‑1 (MQ=255)
                                                                                                                                     ggaggaATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATgg                                                                          >  1:528655/1‑86 (MQ=255)
                                                                                                                                       agtaataatAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCg         >  1:146144/4‑149 (MQ=255)
                                                                                                                                       aggaATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAgt                               <  3:133900/127‑1 (MQ=255)
                                                                                                                                       aggaATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAgt                               >  4:133900/1‑127 (MQ=255)
                                                                                                                                        ggaATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCgg        <  8:291167/149‑1 (MQ=255)
                                                                                                                                         gaATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGc       >  7:165908/1‑149 (MQ=255)
                                                                                                                                         gaATAATAAGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGc       >  7:23951/1‑149 (MQ=255)
                                                                                                                                            taataaGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCAttgtt                                                      <  7:495150/99‑1 (MQ=255)
                                                                                                                                            taataaGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCAttgtt                                                      >  8:495150/1‑99 (MQ=255)
                                                                                                                                            taataaGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGaaa                                   <  2:131438/118‑1 (MQ=255)
                                                                                                                                            taataaGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGaaa                                   >  1:131438/1‑118 (MQ=255)
                                                                                                                                            taataaGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGcc                  >  6:366889/1‑135 (MQ=255)
                                                                                                                                            taataaGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGcc                  <  5:366889/135‑1 (MQ=255)
                                                                                                                                            taataaGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGt    >  3:85529/1‑149 (MQ=255)
                                                                                                                                            taataaGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGt    <  8:41075/149‑1 (MQ=255)
                                                                                                                                              ataaGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCGCAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGttt  <  7:402888/149‑1 (MQ=255)
                                                                                                                                              ataaGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGcc                  <  1:318441/133‑1 (MQ=255)
                                                                                                                                              ataaGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGcc                  >  2:318441/1‑133 (MQ=255)
                                                                                                                                              ataaGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGttt  <  7:323240/149‑1 (MQ=255)
                                                                                                                                              ataaGAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGttt  >  6:358896/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTT  >  NC_000913/4174999‑4175290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: