| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 288,488 | 0 | C | G | 20.0% | 57.6 / 10.0 | 25 | V225V (GTG→GTC) | yagI | CP4‑6 prophage; putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (10/10); new base G (2/3); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.87e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCT > NC_000913/288340‑288634 | gACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTcc > 3:20201/1‑149 (MQ=255) aGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCAcgcg < 6:65777/148‑1 (MQ=255) gACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTGGACGACGCggagctgg < 4:89793/149‑7 (MQ=255) tACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCAcgc < 8:71734/132‑1 (MQ=255) tACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCAcgc > 7:71734/1‑132 (MQ=255) gACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCa > 1:94584/1‑144 (MQ=255) gACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCa < 2:94584/144‑1 (MQ=255) ctTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGAt > 4:121100/1‑149 (MQ=255) aCCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGAc < 4:20201/148‑1 (MQ=255) tAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTgg < 8:122378/146‑1 (MQ=255) tAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTgg > 7:122378/1‑146 (MQ=255) aCGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCca > 2:78281/1‑149 (MQ=255) gggTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGc < 8:111136/93‑1 (MQ=255) gggTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGc > 7:111136/1‑93 (MQ=255) ggTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGc > 1:142215/1‑102 (MQ=255) ggTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGc < 2:142215/102‑1 (MQ=255) agCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTc < 3:121100/149‑1 (MQ=255) cgAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTtc < 2:150863/149‑1 (MQ=255) tgctgAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTc > 4:80625/1‑149 (MQ=255) gctCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGt > 6:102393/1‑147 (MQ=255) gctCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGt < 5:102393/147‑1 (MQ=255) ctcccGCGTGGACGACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGtt < 6:71615/106‑1 (MQ=255) ctcccGCGTGGACGACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGtt > 5:71615/4‑109 (MQ=255) ctcccGCGTGGACGACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAgg < 6:63836/122‑1 (MQ=255) ctcccGCGTGGACGACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAgg > 5:63836/4‑125 (MQ=255) tcCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCt > 6:25752/1‑149 (MQ=255) | GACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCT > NC_000913/288340‑288634 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |