Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,662,481 | C→A | 5.1% | H690N (CAC→AAC) | mdtF → | anaerobic multidrug efflux transporter, ArcA‑regulated |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,662,481 | 0 | C | A | 5.1% | 270.7 / 2.8 | 78 | H690N (CAC→AAC) | mdtF | anaerobic multidrug efflux transporter, ArcA‑regulated |
Reads supporting (aligned to +/- strand): ref base C (35/39); new base A (2/2); total (37/41) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 |
TCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGATGTTCAAAGTGAACGTCAACGCTGCGAAAGCTGAAGCGATG > NC_000913/3662334‑3662624 | tCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCa > 3:160865/1‑149 (MQ=255) aCCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGaaa > 1:416792/1‑148 (MQ=255) cgATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGTTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTa < 6:212169/149‑1 (MQ=255) gATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTaa > 6:181745/1‑149 (MQ=255) aTCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCTGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAAc > 2:252093/1‑149 (MQ=255) aTCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAAc < 7:151841/149‑1 (MQ=255) cATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAAcc > 6:36821/1‑148 (MQ=255) aTTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTTGACAACGGTAACCTGGGGCACGAAAAACTAAccc < 1:210732/148‑1 (MQ=255) aTTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAAccc < 7:113381/148‑1 (MQ=255) cGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGgcgc > 8:230767/1‑148 (MQ=255) cGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGcgcg < 4:129153/149‑1 (MQ=255) gggCAATGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGgc < 7:237789/145‑1 (MQ=255) gggCAATGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGgc > 8:237789/1‑145 (MQ=255) aTGATTGCGTTAAGCAGTATCAATAAAGCAGTCGTCTTACCGTTAAACTTACCCGCGGTGGCTGAACTGGTTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGa > 3:249981/1‑149 (MQ=255) tGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAg > 3:30330/1‑149 (MQ=255) tGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAg < 4:240466/149‑1 (MQ=255) cAGTATCAATAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTgg < 5:181745/149‑1 (MQ=255) gTATCAATAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTgg > 3:332747/1‑147 (MQ=255) tATCAATAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAg > 3:370121/1‑149 (MQ=255) aaTAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAgcgc > 2:76851/1‑148 (MQ=255) aaTAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAgcgc < 2:395324/148‑1 (MQ=255) aTAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTaa < 7:111290/111‑1 (MQ=255) aTAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTaa > 8:111290/1‑111 (MQ=255) tAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGTTTTTGATATGGAACTGCGGGTCAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCCGCGCAAt > 3:245965/1‑149 (MQ=255) aaGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAgcgc > 5:156788/1‑144 (MQ=255) aaGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAgcgc < 6:156788/144‑1 (MQ=255) gCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCAcc > 2:66993/1‑149 (MQ=255) gCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCAcc > 4:23060/1‑149 (MQ=255) gtcTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAAt > 5:186079/1‑147 (MQ=255) gtcTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATc < 7:230767/148‑1 (MQ=255) tcTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAg < 1:252093/149‑1 (MQ=255) tcTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAg < 4:336520/149‑1 (MQ=255) ttCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCa < 1:185575/146‑1 (MQ=255) ttCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCa > 2:185575/1‑146 (MQ=255) cccGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTc < 8:74763/148‑1 (MQ=255) cccGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCa > 1:401321/1‑149 (MQ=255) ccGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCAc > 3:191883/1‑149 (MQ=255) gTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGgc < 8:274432/99‑1 (MQ=255) gTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGgc > 7:274432/1‑99 (MQ=255) aaCTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCgggg < 1:369440/148‑1 (MQ=255) aaCTTACCCGCGGTGGCTGAACT‑GGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGAACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTa > 2:275247/1‑149 (MQ=255) aaCTTACCCGCGGTGGCTGAACT‑GGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGAACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTa > 4:48368/1‑149 (MQ=255) aCCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGcc > 8:269659/1‑149 (MQ=255) cccGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGcc < 4:327314/148‑1 (MQ=255) ccGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGccc > 2:408833/1‑148 (MQ=255) gcgGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGaa < 4:332747/149‑1 (MQ=255) cgGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAAc > 8:71291/1‑149 (MQ=255) tGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGc < 7:269659/149‑1 (MQ=255) aaCTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTgg < 1:76851/147‑1 (MQ=255) aCTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAg < 6:186079/149‑1 (MQ=255) cTGGGTACCGCGTCATGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGa < 4:370121/149‑1 (MQ=255) ggTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATa < 1:408833/148‑1 (MQ=255) ggTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATa < 7:71291/148‑1 (MQ=255) ggTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATa < 2:416792/148‑1 (MQ=255) tACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGc < 3:258908/149‑1 (MQ=255) tACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGc < 4:245965/149‑1 (MQ=255) ccGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGcc > 7:38932/1‑148 (MQ=255) gcgTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGAt > 5:54198/1‑149 (MQ=255) gcgTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGAt > 5:302274/1‑149 (MQ=255) aGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGATGTTc < 2:401321/148‑1 (MQ=255) gTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGATGTTCaaa < 5:36821/149‑1 (MQ=255) gATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGATGTTCAAAGTGaa > 4:361649/1‑149 (MQ=255) atatGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCTCTGGCAGCGCATTCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGATGTTCAAAGTGaa < 4:30330/148‑1 (MQ=255) atatGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGATGTTCAAAGTGAAc > 4:189943/1‑149 (MQ=255) ggAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGa > 3:143428/1‑131 (MQ=255) ggAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGa < 4:143428/131‑1 (MQ=255) gAACTGCTGGACAACGGTAACCTGGGGAACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGATGTTCAAAGTGAACGTCaa < 1:275247/149‑1 (MQ=255) gAACTGCTGGACAACGGTAACCTGGGGAACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGATGTTCAAAGTGAACGTCaa < 3:48368/149‑1 (MQ=255) aaCTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGTCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGATCTTCAAAGTGAACGTCAAc > 4:320575/1‑149 (MQ=255) ctgGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGATGTTCAAAGTGAACGTCAACGCTGc < 3:361649/149‑1 (MQ=255) cccAACGGTATCCTGCGGCACGAAAAACTAACCCAGGCCCGAAACGAGCTGTCATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGATGTTCAAAGTGAACGTCAACTCTGCGa < 4:249981/146‑1 (MQ=255) aaCGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGATGTTCaaa > 6:176515/1‑126 (MQ=255) aaCGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGATGTTCaaa < 5:176515/126‑1 (MQ=255) ggTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGATGTTCAAAGTGAACGTCAACGCTGCGAAAGCTg > 1:368329/1‑148 (MQ=255) gTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGGAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGATGATACGCCGATGTTCAAAGTGAACGTCAACGCTGCGAAAGCTGaa < 4:160865/149‑1 (MQ=255) cTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGATGTTCAAAGTGAACGTCAACGCTGCGAAAGCTGAAGCGAt < 1:66993/149‑1 (MQ=255) cTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGATGTTCAAAGTGAACGTCAACGCTGCGAAAGCTGAAGCGAt > 7:408230/1‑149 (MQ=255) gggCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGATGTTCAAAGTGAACGTCAACGCTGCGAAAGCTGAAGCGATg < 4:191883/147‑1 (MQ=255) | TCGGTTACCGCGATCATTCAGCGGGCAATGATTGCGTTAAGCAGTATCAATAAAGCCGTCGTCTTCCCGTTCAACTTACCCGCGGTGGCTGAACTGGGTACCGCGTCAGGTTTTGATATGGAACTGCTGGACAACGGTAACCTGGGGCACGAAAAACTAACCCAGGCGCGAAACGAGCTGTTATCACTGGCAGCGCAATCACCGAATCAGGTCACCGGGGTACGCCCGAACGGCCTGGAAGATACGCCGATGTTCAAAGTGAACGTCAACGCTGCGAAAGCTGAAGCGATG > NC_000913/3662334‑3662624 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |