Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,148,199 C→T 5.2% L176L (CTG→TTG)  yegD → Hsp70 chaperone family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,148,1990CT 5.2% 248.9 / 2.4 76L176L (CTG→TTG) yegDHsp70 chaperone family protein
Reads supporting (aligned to +/- strand):  ref base C (36/36);  new base T (2/2);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

GAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTTCA  >  NC_000913/2148052‑2148345
                                                                                                                                                   |                                                                                                                                                  
gAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAACCCCCCAGGCGCAAGGGATTCt                                                                                                                                                   >  7:195738/1‑149 (MQ=255)
gAGGATCTGGGCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTc                                                                                                                                                    <  3:305278/148‑1 (MQ=255)
 aGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTg                                                                                                                                                  >  7:110575/1‑149 (MQ=255)
   gATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTgg                                                                                                                                                 >  2:316298/1‑148 (MQ=255)
   gATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGa                                                                                                                                                <  5:34061/149‑1 (MQ=255)
    aTCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGaa                                                                                                                                               >  7:133121/1‑149 (MQ=255)
         gTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAAc                                                                                                                                              <  7:302598/145‑1 (MQ=255)
               gcAATGATGTTGCACATTCGCCAGCAGGGGCAGGCACAGCTGCCTGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCAGCGaa                                                                                                                                    <  2:365041/149‑1 (MQ=255)
               gcAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCgcggcg                                                                                                                                      >  2:261384/1‑147 (MQ=255)
               gcAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGaa                                                                                                                                    <  2:186425/149‑1 (MQ=255)
                  atgatgTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCg                                                                                                                                 <  8:133121/149‑1 (MQ=255)
                   tgatgTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGt                                                                                                                                <  6:371980/149‑1 (MQ=255)
                          gCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGACGCGATTACTCAGGCGGTGATTGGTCGTCCGATCCACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGAt                                                                                                                         >  3:378671/1‑149 (MQ=255)
                            acaTTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGAtt                                                                                                                        >  6:347409/1‑148 (MQ=255)
                             caTTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCa                                                                                                                      <  8:110575/149‑1 (MQ=255)
                              aTTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAg                                                                                                                     >  2:249560/1‑149 (MQ=255)
                              aTTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAg                                                                                                                     <  1:249560/149‑1 (MQ=255)
                              aTTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAg                                                                                                                     <  6:63721/149‑1 (MQ=255)
                                  gCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAAAGCTCGGCGAAGCGTGCCGGATTCAGTGAc                                                                                                                 >  2:132054/1‑149 (MQ=255)
                                     agcagGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTg                                                                                                              <  5:385507/149‑1 (MQ=255)
                                      gcaggcgcaggcACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCa                                                                                                                      >  4:102432/1‑140 (MQ=255)
                                      gcaggcgcaggcACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCa                                                                                                                      <  3:102432/140‑1 (MQ=255)
                                          gcgcaggcACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTAtt                                                                                                         >  3:90884/1‑149 (MQ=255)
                                            gcaggcACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTcc                                                                                                       <  2:315778/149‑1 (MQ=255)
                                                gcACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTa                                                                                                   >  3:66408/1‑149 (MQ=255)
                                                      cTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGcc                                                                                             <  8:135010/149‑1 (MQ=255)
                                                           aGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTcg                                                                                        >  2:387107/1‑149 (MQ=255)
                                                            gAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTg                                                                                                                                                  >  7:86561/1‑90 (MQ=255)
                                                            gAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTg                                                                                                                                                  <  8:86561/90‑1 (MQ=255)
                                                            gAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCAGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTcgc                                                                                       >  4:156598/1‑149 (MQ=255)
                                                               gCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCggc                                                                                    >  5:277093/1‑149 (MQ=255)
                                                                       tCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCggctgggctg                                                                             >  3:210915/1‑148 (MQ=255)
                                                                       tCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCggctgggctg                                                                             >  7:91308/1‑148 (MQ=255)
                                                                         aGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGa                                                                           <  5:347409/148‑1 (MQ=255)
                                                                          ggCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGAtt                                                                         <  5:368944/149‑1 (MQ=255)
                                                                           gCGGTGATTGGTGGGCCGATCTACTTCCAGGGGCTGGGCGGTTATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGGGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTa                                                                        <  2:162378/149‑1 (MQ=255)
                                                                           gCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTa                                                                        <  4:90884/149‑1 (MQ=255)
                                                                              gTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCAGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGa                                                                     <  3:156598/149‑1 (MQ=255)
                                                                               tGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGaa                                                                    <  1:316298/149‑1 (MQ=255)
                                                                                      tcgtcCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTTTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCt                                                             >  7:166076/1‑149 (MQ=255)
                                                                                        gtcCGATCAACTTCCAGGGGCTGGGCGTTCATGAAACAAACACCCAGGCGCAAGGGATTCTGGAACGAGCGGAGAAGCGTGACGGATTCAGGGACGTGGTATTACACTACGAGCCGGTCGCgcctgggc                                                                               >  4:222837/1‑129 (MQ=255)
                                                                                        gtcCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGTGACGTGGTATTCCAGTACGAGCCGGTCGCggctgggc                                                                               <  3:222837/129‑1 (MQ=255)
                                                                                         tcCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCAc                                                               >  2:12122/1‑144 (MQ=255)
                                                                                         tcCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGc                                                          <  6:277093/149‑1 (MQ=255)
                                                                                         tcCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGGGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCAc                                                               <  1:12122/144‑1 (MQ=255)
                                                                                          ccGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCa                                                         <  1:387107/149‑1 (MQ=255)
                                                                                          ccGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGAATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCa                                                         <  1:261384/149‑1 (MQ=255)
                                                                                            gATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAg                                                        >  7:62917/1‑148 (MQ=255)
                                                                                             aTCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTTTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAgg                                                       >  2:137059/1‑148 (MQ=255)
                                                                                             aTCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAg                                                                                                                     >  5:86943/1‑86 (MQ=255)
                                                                                             aTCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAg                                                                                                                     <  6:86943/86‑1 (MQ=255)
                                                                                             aTCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAgg                                                       >  1:378642/1‑148 (MQ=255)
                                                                                             aTCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGga                                                      <  8:62917/149‑1 (MQ=255)
                                                                                                  cTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGgaagaa                                                  >  2:215642/1‑148 (MQ=255)
                                                                                                     ccAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTcgc                                                                                       <  4:95105/108‑1 (MQ=255)
                                                                                                     ccAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTcgc                                                                                       >  3:95105/1‑108 (MQ=255)
                                                                                                       aGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACgg                                            >  8:236398/1‑149 (MQ=255)
                                                                                                       aGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACgg                                            <  8:91308/149‑1 (MQ=255)
                                                                                                       aGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACgg                                            <  2:378642/149‑1 (MQ=255)
                                                                                                           gCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTTTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGc                                        <  8:166076/149‑1 (MQ=255)
                                                                                                            cTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTTTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCt                                       <  1:137059/149‑1 (MQ=255)
                                                                                                             tGGGCGGTGATGATGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCtg                                      <  1:132054/149‑1 (MQ=255)
                                                                                                                   gcgctgcaGCACACACCCAGGCGCAAGGGATGCGCGAACGCGCGGCGAAGCGGGCCCGACTCAGGGACGCCCTAATCCAGAACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGCGCtggtggtg                                <  8:315834/142‑1 (MQ=255)
                                                                                                                          aGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATcg                         >  5:362472/1‑149 (MQ=255)
                                                                                                                             aaaCACCCAGGCGCAAGGGGTTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGGATTCCAGTACGAGCCGGGCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATcggcg                      <  2:44683/149‑1 (MQ=255)
                                                                                                                                   ccAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGAGTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTa                >  6:262128/1‑149 (MQ=255)
                                                                                                                                    cAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGGGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGGCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTAc               <  8:195738/149‑1 (MQ=255)
                                                                                                                                     aGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCtt                                                            >  8:406558/1‑103 (MQ=255)
                                                                                                                                     aGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGata                            >  7:337664/1‑135 (MQ=255)
                                                                                                                                     aGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGata                            <  8:337664/135‑1 (MQ=255)
                                                                                                                                       gcgcAAGGGATTCTGGAACGCGCGGCGAAGCGTGTCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGa                                                                           <  5:166076/86‑1 (MQ=255)
                                                                                                                                       gcgcAAGGGATTCTGGAACGCGCGGCGAAGCGTGTCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGa                                                                           >  6:166076/1‑86 (MQ=255)
                                                                                                                                            aGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACgactgact       >  2:18837/1‑149 (MQ=255)
                                                                                                                                            aGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACgactgact       >  1:180694/1‑149 (MQ=255)
                                                                                                                                             gggATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGactgactg      >  5:395019/1‑149 (MQ=255)
                                                                                                                                               gATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGtt    >  1:362643/1‑149 (MQ=255)
                                                                                                                                                 ttCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTTCa  >  8:182032/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTTCA  >  NC_000913/2148052‑2148345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: