Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,413,966 T→A 5.3% intergenic (‑39/+18) ralR ← / ← recT Rac prophage; restriction alleviation protein/Rac prophage; recombination and repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,413,9660TA 5.3% 372.7 / 8.1 113intergenic (‑39/+18)ralR/recTRac prophage; restriction alleviation protein/Rac prophage; recombination and repair protein
Reads supporting (aligned to +/- strand):  ref base T (51/56);  new base A (2/4);  total (53/60)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

Only 100 of 118 total aligned reads displayed.

GTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATATT  >  NC_000913/1413818‑1414114
                                                                                                                                                    |                                                                                                                                                    
 ttCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCAtt                                                                                                                                                     <  2:157182/149‑1 (MQ=255)
      gATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACAcc                                                                                                                                                <  3:335572/149‑1 (MQ=255)
           gCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCa                                                                                                                                            <  4:270330/148‑1 (MQ=255)
           gCATCCGTTACACTTCGCTCGGTAATATCCTGAAATCGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAAGTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaa                                                                                                                                           <  5:252255/149‑2 (MQ=255)
            cATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaac                                                                                                                                          <  2:359532/149‑3 (MQ=255)
              tCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCt                                                                                                                                        >  1:108196/1‑149 (MQ=255)
                 gTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaac                                                                                                                                          >  4:222518/1‑142 (MQ=255)
                 gTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaac                                                                                                                                          <  3:222518/144‑3 (MQ=255)
                 gTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGaa                                                                                                                                     >  3:323780/1‑149 (MQ=255)
                 gTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGaa                                                                                                                                     >  2:332404/1‑149 (MQ=255)
                     cacTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAatta                                                                                                                                  <  1:221741/148‑1 (MQ=255)
                      acTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCAACTTGAaatatt                                                                                                                                >  3:250364/1‑149 (MQ=255)
                       cTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTc                                                                                                                               <  8:191662/149‑1 (MQ=255)
                        ttCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTcc                                                                                                                              >  2:126220/1‑149 (MQ=255)
                           gCTCGGTAATGTCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCctct                                                                                                                           <  1:289482/149‑1 (MQ=255)
                           gCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCctct                                                                                                                           <  6:386679/149‑1 (MQ=255)
                             tCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTg                                                                                                                          >  3:187274/1‑148 (MQ=255)
                             tCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGAAATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCAGTGCATTACACCGCCAGTCTGAATTATTCCTCTg                                                                                                                          >  7:267261/1‑148 (MQ=255)
                             tCGGTAATATCCGGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACAGTGTCATATCTCATAATTTTTCTCATAAAAAATATTTAAAGTTGGCGGGTCATTACACCGCCAGGCTGAATTATTCCTCTg                                                                                                                          >  8:236077/1‑148 (MQ=255)
                               ggTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAAt                                                                                                                       >  6:160004/1‑149 (MQ=255)
                               ggTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTAAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGaa                                                                                                                        <  1:42533/148‑1 (MQ=255)
                                gTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAAt                                                                                                                       >  7:109874/1‑148 (MQ=255)
                                   atatCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATc                                                                                                                   <  6:98525/149‑1 (MQ=255)
                                      tCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGa                                                                                                                 <  2:108196/148‑1 (MQ=255)
                                      tCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGa                                                                                                                 <  8:109874/148‑1 (MQ=255)
                                      tCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAt                                                                                                                >  7:50017/1‑149 (MQ=255)
                                      tCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAt                                                                                                                >  7:162705/1‑149 (MQ=255)
                                      tCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTAATCCTCTGAATTATCGAt                                                                                                                <  2:236003/149‑1 (MQ=255)
                                       ccTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTGAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAt                                                                                                                >  2:87099/1‑148 (MQ=255)
                                        cTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAt                                                                                                                >  1:381403/1‑147 (MQ=255)
                                        cTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAt                                                                                                                <  2:381403/147‑1 (MQ=255)
                                        cTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTa                                                                                                              <  1:146394/149‑1 (MQ=255)
                                        cTGAAATGGCTTTACCCGTTACTGATTGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTa                                                                                                              <  7:414061/149‑1 (MQ=255)
                                           aaaTGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTacac                                                                                                           >  6:390198/1‑149 (MQ=255)
                                           aaaTGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTacac                                                                                                           >  5:187793/1‑149 (MQ=255)
                                            aaTGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACt                                                                                                          <  4:323780/149‑1 (MQ=255)
                                               ggCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATGTCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTa                                                                                                       >  4:227311/1‑149 (MQ=255)
                                                  tttCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTc                                                                                                    >  4:229261/1‑149 (MQ=255)
                                                     cACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTccc                                                                                                  <  4:156112/148‑1 (MQ=255)
                                                      aCCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACCCCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCg                                                                                                >  8:133402/1‑149 (MQ=255)
                                                             cTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATa                                                                                         <  4:327489/149‑1 (MQ=255)
                                                             cTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATa                                                                                         <  3:350055/149‑1 (MQ=255)
                                                              tGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCg                                                                                                                  <  5:68872/123‑1 (MQ=255)
                                                              tGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCg                                                                                                                  >  6:68872/1‑123 (MQ=255)
                                                                aTGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACag                                                                                      <  6:295213/149‑1 (MQ=255)
                                                                 tGGACAACCAGAAAATGGACATGGTTTAACATTGTCATATCTTATAATTTTTCTCATATAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGATTTATCGATTACACTTTATTCCCCGGTTAATACaga                                                                                     <  8:267261/149‑1 (MQ=255)
                                                                 tGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACaga                                                                                     <  8:162705/149‑1 (MQ=255)
                                                                   gACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACagag                                                                                    <  8:340727/148‑1 (MQ=255)
                                                                    aCAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGa                                                                                  <  3:199509/149‑1 (MQ=255)
                                                                        ccACAAAATGGACATGGTTTAACATTGTCATATGTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTTCACTGTATTCGACGGTTAATACAGAGGAATCt                                                                              >  2:76928/1‑149 (MQ=255)
                                                                        ccACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCt                                                                              >  3:150191/1‑149 (MQ=255)
                                                                         cacaAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTg                                                                             >  8:191845/1‑149 (MQ=255)
                                                                            aaaaTGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAg                                                                          >  5:184205/1‑149 (MQ=255)
                                                                                  gACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAt                                                                                                                >  6:343004/1‑105 (MQ=255)
                                                                                  gACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAt                                                                                                                <  5:343004/105‑1 (MQ=255)
                                                                                  gACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGa                                                                    <  6:210319/149‑1 (MQ=255)
                                                                                   aCATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGAt                                                                   <  7:313148/149‑1 (MQ=255)
                                                                                    cATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGAtt                                                                  <  5:340620/149‑1 (MQ=255)
                                                                                       ggTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACCCCGCCAGGCTGAATTATTCCTCTGAATTATCGATTTCACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGt                                                                >  2:190240/1‑148 (MQ=255)
                                                                                         tttAACATTGTCATCTCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAg                                                             <  3:227311/149‑1 (MQ=255)
                                                                                          ttAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCACTGAATTATCGATTACACTGTATTCCCCGCTTAATACAGAGGAATCTGCAGGATCGATTGTCAGt                                                            >  2:64349/1‑149 (MQ=255)
                                                                                            taCATTGTCATATCTCTTAATTTTTCTCATAAAAAATATTTCATGTTGGCGGTGCTTTACACTGCCAGTCTGAATTTTTCCTCTGATTTATCTATTACAGTGTATTCCCCGGGTAATACAGAGGAATCTGGAGGATGGATTGTCAGTg                                                           <  8:344800/147‑1 (MQ=255)
                                                                                             aCATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGt                                                         <  4:150191/149‑1 (MQ=255)
                                                                                              cATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGtt                                                        <  6:184205/149‑1 (MQ=255)
                                                                                               tttGTCATATCTAATAATTTTTATCATAAAAAATATTTCAAGTTGGCGGTTAATTACACCGCCAGGCTGAATTATTCCTCTGATTTATCGATTACACTGTATTCCCCGGTTAATACa                                                                                       <  2:340938/116‑1 (MQ=255)
                                                                                               aTTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGACTACACTGTATTCCCCGGTTAATACAGAGGa                                                                                  >  3:295939/1‑122 (MQ=255)
                                                                                               aTTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTACTCTGAATTATCGATTACACTGTATTCCCCGGTTAATaaa                                                                                       >  1:340938/1‑115 (MQ=255)
                                                                                                ttGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTcc                                                      >  4:288909/1‑149 (MQ=255)
                                                                                                      tatCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCa                                                <  6:187793/149‑1 (MQ=255)
                                                                                                      tatCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGACAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCa                                                >  7:323035/1‑149 (MQ=255)
                                                                                                       atCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCAt                                               >  4:216775/1‑149 (MQ=255)
                                                                                                          tcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTccc                                                                                                  >  4:125333/1‑95 (MQ=255)
                                                                                                          tcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCa                                            >  2:406250/1‑149 (MQ=255)
                                                                                                            aTAATTTTTCTCTTAAAATATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTGCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCAtt                                          <  5:391095/149‑1 (MQ=255)
                                                                                                             tAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTTAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTg                                         <  5:160004/149‑1 (MQ=255)
                                                                                                             tAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACt                                                                                                          <  7:101690/84‑1 (MQ=255)
                                                                                                             tAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACt                                                                                                          >  8:101690/1‑84 (MQ=255)
                                                                                                             aaaTTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGATTTATTCCTATGATTTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTTCATGATCGATTGTCAGTGGATCAATTTCATCCAtt                                          >  7:372992/2‑148 (MQ=255)
                                                                                                               aTTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGa                                        <  1:126220/148‑1 (MQ=255)
                                                                                                                tttttCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGAt                                       >  1:152548/1‑148 (MQ=255)
                                                                                                                   ttCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACt                                    <  8:50017/148‑1 (MQ=255)
                                                                                                                        aTAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGc                              >  6:352198/1‑149 (MQ=255)
                                                                                                                         tAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGAt                                                                   <  1:41344/111‑1 (MQ=255)
                                                                                                                         tAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGAt                                                                   >  2:41344/1‑111 (MQ=255)
                                                                                                                         tAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCt                             >  4:113445/1‑149 (MQ=255)
                                                                                                                         tAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCt                             >  2:68341/1‑149 (MQ=255)
                                                                                                                           aaaaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTgg                           <  2:152548/149‑1 (MQ=255)
                                                                                                                             aaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTTATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGAt                         >  2:216553/1‑149 (MQ=255)
                                                                                                                              aaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTCTTCCTCTGAATTATCGATTACACTGTATTCCCCGGtt                                                                                             <  6:274534/80‑1 (MQ=255)
                                                                                                                              aaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACATAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGAtc                        <  1:87099/149‑1 (MQ=255)
                                                                                                                              aaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGAtc                        >  3:323006/1‑149 (MQ=255)
                                                                                                                              aaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCACGGtt                                                                                             >  5:274534/1‑80 (MQ=255)
                                                                                                                                  tttCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCaa                    >  1:18757/1‑149 (MQ=255)
                                                                                                                                     cAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTTAATTATCGATTACACTGTATTCCCCGGTTAATAGAGAGTAATCTGCAGTATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAAtt                  <  1:64349/148‑1 (MQ=255)
                                                                                                                                        gTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATa              <  2:243577/149‑1 (MQ=255)
                                                                                                                                        gTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATa              <  4:187274/149‑1 (MQ=255)
                                                                                                                                        gTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATa              <  5:284869/149‑1 (MQ=255)
                                                                                                                                               gTGCATTACACCGCCAGGCTGAATTATTCCTCTGAAGTATCGATTACACTCTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCa        >  2:135076/1‑148 (MQ=255)
                                                                                                                                                    ttACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATa              >  2:77765/1‑137 (MQ=255)
                                                                                                                                                    ttACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATAtt  >  1:97617/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATATT  >  NC_000913/1413818‑1414114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑