Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,231,608 G→C 8.2% Q141H (CAG→CAC umuC → translesion error‑prone DNA polymerase V subunit; DNA polymerase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,231,6080GC 8.2% 270.8 / 9.9 81Q141H (CAG→CACumuCtranslesion error‑prone DNA polymerase V subunit; DNA polymerase activity
Reads supporting (aligned to +/- strand):  ref base G (34/40);  new base C (4/3);  total (38/43)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGA  >  NC_000913/1231462‑1231756
                                                                                                                                                   |                                                                                                                                                    
ccccGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGAc                                                                                                                                                    >  5:136041/1‑149 (MQ=255)
 cccGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGTATCGCCCAGAc                                                                                                                                                    >  6:53141/1‑148 (MQ=255)
  ccGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGAcc                                                                                                                                                   >  5:76340/1‑148 (MQ=255)
     cgTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTCTGGGCGATCCCCACACCaaca                                                                                                                                               <  3:171118/149‑3 (MQ=255)
       tCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACg                                                                                                                                             >  3:87088/1‑149 (MQ=255)
          agaTTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTg                                                                                                                                          <  7:95693/149‑1 (MQ=255)
           gaTTTACAGTATTGATGAGGCTTTCTGCGATCTGACAGGTGTGCGTTATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTg                                                                                                                                          <  6:365762/148‑1 (MQ=255)
               tACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTCTGGGCGATCCCCACACCaaca                                                                                                                                               <  7:109343/139‑3 (MQ=255)
               tACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTCTGGGCGATCCCCACACCaaca                                                                                                                                               >  8:109343/1‑137 (MQ=255)
                aCAGTATTGATGCGGCATTCTGCGATCTGACAGGGGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTaa                                                                                                                                     <  1:273691/148‑1 (MQ=255)
                   gTATTGATGAGGCATTCTGCGAGCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCtt                                                                                                                                 <  8:212420/149‑1 (MQ=255)
                            aGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa                                                                                                                         <  6:76340/148‑1 (MQ=255)
                            aGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa                                                                                                                         <  2:250418/148‑1 (MQ=255)
                            aGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa                                                                                                                         <  8:182337/148‑1 (MQ=255)
                             ggCATTCTGCGATCTGACATGTGTGCGTAATTGTCGCGATCTGACTGATTTTGTCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATg                                                                                                                       <  8:1206/149‑1 (MQ=255)
                             ggCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTCTGGGCGATCCCCACACCaacag                                                                                                                                              <  4:55894/126‑4 (MQ=255)
                             ggCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTCTGGGCGATCCCCACACCaacag                                                                                                                                              >  3:55894/1‑123 (MQ=255)
                              gCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACATCGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGc                                                                                                                      <  2:346421/149‑1 (MQ=255)
                              gCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGc                                                                                                                      >  3:21940/1‑149 (MQ=255)
                               cATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACTGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCg                                                                                                                     <  1:253696/149‑1 (MQ=255)
                                 ttCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATc                                                                                                                          <  3:59393/142‑1 (MQ=255)
                                 ttCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATc                                                                                                                          >  4:59393/1‑142 (MQ=255)
                                 ttCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa                                                                                                                         >  4:81772/1‑143 (MQ=255)
                                 ttCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa                                                                                                                         <  3:81772/143‑1 (MQ=255)
                                   cTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCa                                                                                                                  >  2:271203/1‑148 (MQ=255)
                                    tGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCaa                                                                                                                 >  6:207271/1‑148 (MQ=255)
                                        aTCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCaaaaaaa                                                                                                            >  2:300577/1‑149 (MQ=255)
                                             aCAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCaaaaaaa                                                                                                            <  8:339729/144‑1 (MQ=255)
                                             aCAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCaaaaaaa                                                                                                            <  3:266965/144‑1 (MQ=255)
                                             aCAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAAt                                                                                                           <  6:323158/145‑1 (MQ=255)
                                             aCAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATg                                                                                                          <  5:207271/146‑1 (MQ=255)
                                             aCAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCa                                                                                                       <  1:300577/149‑1 (MQ=255)
                                              cAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAg                                                                                                      <  1:403380/149‑1 (MQ=255)
                                               aGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGc                                                                                                     <  8:219964/149‑1 (MQ=255)
                                                    tgCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa                                                                                                                         >  6:344899/1‑124 (MQ=255)
                                                    tgCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa                                                                                                                         <  5:344899/124‑1 (MQ=255)
                                                    tgCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAg                                                                                                >  5:271007/1‑149 (MQ=255)
                                                      cGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGAc                                                                                              >  8:318620/1‑149 (MQ=255)
                                                        tAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACgg                                                                                            >  8:353210/1‑149 (MQ=255)
                                                          aTTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACgggt                                                                                          <  8:214804/149‑1 (MQ=255)
                                                           ttGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACgggtg                                                                                         <  2:189970/149‑1 (MQ=255)
                                                              tCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACgggtggg                                                                                       <  7:44717/148‑1 (MQ=255)
                                                              tCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACgggtggg                                                                                       <  4:21940/148‑1 (MQ=255)
                                                                gcgATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCaa                                                                                                                                                 >  7:352234/1‑88 (MQ=255)
                                                                gcgATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCaa                                                                                                                                                 <  8:352234/88‑1 (MQ=255)
                                                                      tgactgaTTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCACACAAAAACGCAGGCTAAGCTACCCAATCATGCGGCAAAAAAATGGAAGCGGCATACGGCTGGGGATGTGGAt                                                                              >  3:44575/1‑149 (MQ=255)
                                                                        actgaTTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGAttt                                                                            <  4:80409/149‑1 (MQ=255)
                                                                        actgaTTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGGTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGa                                                                               <  6:134577/146‑1 (MQ=255)
                                                                                 ggCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGTATCGCCCAGACCAAAACGCTGGCTAAGGTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATc                                                                    <  5:53141/148‑1 (MQ=255)
                                                                                 ggCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCAAAGCTTGCCAATAATGCGGCAAAAAAATGGCATCGGCAGACGGGTGGGTTGGTGGATTTATCAAAtat                                                                   >  6:280627/1‑147 (MQ=255)
                                                                                  gCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCt                                                                   <  6:136041/148‑1 (MQ=255)
                                                                                   cAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTg                                                                  >  2:403057/1‑148 (MQ=255)
                                                                                    agagAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGggtggt                                                                                  <  1:75700/131‑1 (MQ=255)
                                                                                    agagAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGggtggt                                                                                  >  2:75700/1‑131 (MQ=255)
                                                                                     gagaAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGaa                                                               >  3:10828/1‑149 (MQ=255)
                                                                                          aTTCGCGCAACGGTGCTACATGGTACGCATCTTAATGTTTGTTTGGGGATCGCACAGACCAAATCGCGTGCTATGATTGCCATTCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTTGTGGATTTATGATATCTGGAACGCCa                                                          <  4:44575/149‑1 (MQ=255)
                                                                                          aTTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGCCCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGc                                                            >  8:336709/1‑147 (MQ=255)
                                                                                                gcAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTa                                                     >  2:50471/1‑148 (MQ=255)
                                                                                                   aCGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGAAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAAt                                                  >  1:294980/1‑148 (MQ=255)
                                                                                                     ggTGCTACCACGTATCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTaa                                               <  5:183375/149‑1 (MQ=255)
                                                                                                      gTGCTACAACGAACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAAt                                              <  3:9531/149‑1 (MQ=255)
                                                                                                       tGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATg                                             >  6:403056/1‑149 (MQ=255)
                                                                                                                      aTCTTACAGTTGGTCTGGGGATATACCACACCAAACCTCTGGCTAAGCTTGAAAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGCGAGGTGTATTTATCAAATCTCTAACGCCAGCGTAAATTAATTTATGCTCACCCAGTg                              >  2:279478/1‑149 (MQ=255)
                                                                                                                       tcataatGTTGGTGTGGCGATCGCCCAGACCAAAACGCAGGCTAAGCTTGCCAATCATTCGGCAAAAAAATGGCAGCGGCAGACGGGTGTGGTGGTGGATTTATCAAATCTGTAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTgg                             <  2:294980/143‑1 (MQ=255)
                                                                                                                       tCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTg                              >  7:54113/1‑148 (MQ=255)
                                                                                                                       tCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTg                              >  7:71572/1‑148 (MQ=255)
                                                                                                                           aCTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGa                            <  7:353210/146‑1 (MQ=255)
                                                                                                                            cTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGa                         <  8:71572/148‑1 (MQ=255)
                                                                                                                               ttGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTc                     >  7:64604/1‑149 (MQ=255)
                                                                                                                               ttGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTc                     <  7:318620/149‑1 (MQ=255)
                                                                                                                                   tgtgGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGAtt                                                                             >  1:40264/1‑89 (MQ=255)
                                                                                                                                   tgtgGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGAtt                                                                             <  2:40264/89‑1 (MQ=255)
                                                                                                                                    gtgGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAACATGGCAGCGGCAGCCGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCGCCGTGGAGGCCGTCTgggg                >  4:145798/1‑149 (MQ=255)
                                                                                                                                    gtgGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTgggg                >  1:401229/1‑149 (MQ=255)
                                                                                                                                     tgGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCAGTGGATGACGTCTgggg                >  8:98410/1‑148 (MQ=255)
                                                                                                                                     tgGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTATGCTCTCCCCGTGGATGACGTCTgggg                <  3:140273/148‑1 (MQ=255)
                                                                                                                                     tgGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAAGGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTgggg                >  1:110934/1‑148 (MQ=255)
                                                                                                                                          gATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTg           >  8:390836/1‑149 (MQ=255)
                                                                                                                                               cccAGCCCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGAcgg      >  5:197621/1‑149 (MQ=255)
                                                                                                                                                 cAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGAt             >  4:216890/1‑139 (MQ=255)
                                                                                                                                                 cAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGAt             <  3:216890/139‑1 (MQ=255)
                                                                                                                                                   gACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGa  <  1:403057/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGA  >  NC_000913/1231462‑1231756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: