Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,048,319 Δ1 bp 5.1% coding (3382/7077 nt) yeeJ → putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,048,3190A. 5.1% 261.9 / 6.2 78coding (3382/7077 nt)yeeJputative adhesin
Reads supporting (aligned to +/- strand):  ref base A (42/32);  new base . (2/2);  total (44/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

ACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGA  >  NC_000913/2048172‑2048464
                                                                                                                                                   |                                                                                                                                                 
aCGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGtaa                                                                                                                                                  <  4:270783/149‑1 (MQ=255)
aCGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGta                                                                                                                                                   <  5:382503/148‑1 (MQ=255)
      aaCTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACaa                                                                                                                                            <  8:270994/149‑1 (MQ=255)
      aaCTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACaa                                                                                                                                            >  3:277758/1‑149 (MQ=255)
      aaCTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACaa                                                                                                                                            <  4:358738/149‑1 (MQ=255)
        cTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTCCCGCACCGCTGGGTAATAACAATa                                                                                                                                          >  5:73557/1‑149 (MQ=255)
           cACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATAc                                                                                                                                         <  2:65968/147‑1 (MQ=255)
              cATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTg                                                                                                                                    <  2:5832/149‑1 (MQ=255)
                tGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGAt                                                                                                                                  >  7:204712/1‑149 (MQ=255)
                      aGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAg                                                                                                                            >  5:392985/1‑149 (MQ=255)
                       ggACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCAAGCATACGGTTACCGCAACGCTGTGTAATAACAATACCAGTGATTCGCAGc                                                                                                                           >  2:341898/1‑149 (MQ=255)
                                ggCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACAt                                                                                                                  >  1:48573/1‑149 (MQ=255)
                                ggCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACAt                                                                                                                  >  1:357928/1‑149 (MQ=255)
                                   aaaCTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGTGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTtg                                                                                                               >  2:384252/1‑149 (MQ=255)
                                   aaaCTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTtg                                                                                                               >  5:231097/1‑149 (MQ=255)
                                    aaCTTTACACTTGAAAATAACGGTATTGCCATCACTCATGCCAATGTGGAAGCGCATGTCACGCTGAAAGGTTAAAAAGCGGTCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTtgt                                                                                                              <  6:73557/149‑1 (MQ=255)
                                      cTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTgg                                                                                                            >  7:44265/1‑149 (MQ=255)
                                         tACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGc                                                                                                           <  4:73997/147‑1 (MQ=255)
                                            ccTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGAc                                                                                                       >  4:64701/1‑148 (MQ=255)
                                            ccTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGAc                                                                                                       >  7:102416/1‑148 (MQ=255)
                                            ccTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGAc                                                                                                       >  7:13401/1‑148 (MQ=255)
                                             cTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAGCGCTGGGTAATAACAATACCAGTGATTGGCTGCCGGTACCAGTTGTGGCGGACa                                                                                                      >  4:240441/1‑148 (MQ=255)
                                              ttGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACa                                                                                                      >  1:63997/1‑147 (MQ=255)
                                              ttGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACa                                                                                                      >  6:375650/1‑147 (MQ=255)
                                              ttGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGAGGACa                                                                                                      >  3:329647/1‑147 (MQ=255)
                                               tGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAg                                                                                                   >  1:178208/1‑149 (MQ=255)
                                                gAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACaaa                                                                                                    <  6:181488/147‑1 (MQ=255)
                                                gAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGGAACAACGCGTGCACGAATACTGTCACCGCAACGCTGTGTAATAAAAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACaaa                                                                                                    <  7:287888/147‑1 (MQ=255)
                                                   aaTAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTc                                                                                               <  8:13401/149‑1 (MQ=255)
                                                     tAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCgg                                                                                             <  3:64701/149‑1 (MQ=255)
                                                         ggTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCt                                                                                           >  4:86951/1‑147 (MQ=255)
                                                           tATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAgg                                                                                       >  8:240436/1‑149 (MQ=255)
                                                           tATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAgg                                                                                       <  2:364518/149‑1 (MQ=255)
                                                                ccATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTc                                                                                  <  6:231097/149‑1 (MQ=255)
                                                                ccATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTc                                                                                  >  5:62113/1‑149 (MQ=255)
                                                                ccATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTc                                                                                  >  5:284578/1‑149 (MQ=255)
                                                                 cATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTcc                                                                                 >  2:227148/1‑149 (MQ=255)
                                                                 cATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTcc                                                                                 >  7:8175/1‑149 (MQ=255)
                                                                 cATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTcc                                                                                 >  7:101815/1‑149 (MQ=255)
                                                                 cATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTcc                                                                                 >  5:292305/1‑149 (MQ=255)
                                                                   tCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACa                                                                                                                                             <  2:62774/87‑1 (MQ=255)
                                                                   tCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACa                                                                                                                                             >  1:62774/1‑87 (MQ=255)
                                                                     aCTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCa                                                                             <  7:240436/149‑1 (MQ=255)
                                                                     aCTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCa                                                                             <  1:227148/149‑1 (MQ=255)
                                                                          ggCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCgg                                                                                             <  4:132500/128‑1 (MQ=255)
                                                                              aaTGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCaa                                                                     >  7:260777/1‑148 (MQ=255)
                                                                                  gggAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGAt                                                                <  5:104273/149‑1 (MQ=255)
                                                                                  gggAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGAt                                                                <  3:86951/149‑1 (MQ=255)
                                                                                    gAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATga                                                              <  4:277758/149‑1 (MQ=255)
                                                                                      aGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATgaga                                                            <  2:48573/149‑1 (MQ=255)
                                                                                       gcgcATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGAt                                                                >  8:148625/1‑144 (MQ=255)
                                                                                       gcgcATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGAt                                                                >  8:154373/1‑144 (MQ=255)
                                                                                       gcgcATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGAt                                                                >  1:12295/1‑144 (MQ=255)
                                                                                           aTGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATcaca                                                       <  2:178208/149‑1 (MQ=255)
                                                                                           aTGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATcaca                                                       <  8:102416/149‑1 (MQ=255)
                                                                                            tGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTAACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAg                                                      >  1:118433/1‑149 (MQ=255)
                                                                                                aCGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTaa                                                  >  4:121200/1‑149 (MQ=255)
                                                                                                  gCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGTGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATg                                                <  3:278896/149‑1 (MQ=255)
                                                                                                  gCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATg                                                <  6:62113/149‑1 (MQ=255)
                                                                                                   cTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATgg                                               <  8:44265/149‑1 (MQ=255)
                                                                                                    tGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATACGCAGCGGGTAACATTTGTGGCGGACAAAGCCGCGGCTGAGGTTGTCGTGCAGATATCTAAAGATGAGATCACAGGTAATGGc                                              >  8:123182/1‑149 (MQ=255)
                                                                                                     gAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCg                                             >  5:350394/1‑149 (MQ=255)
                                                                                                         ggTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGT‑ATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGa                                         >  8:170953/1‑148 (MQ=255)
                                                                                                         ggTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGT‑ATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGa                                         >  6:346069/1‑148 (MQ=255)
                                                                                                          gTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGa                                         >  4:131286/1‑148 (MQ=255)
                                                                                                          gTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGT‑ATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATa                                       <  5:346069/149‑1 (MQ=255)
                                                                                                          gTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGT‑ATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATa                                       <  7:170953/149‑1 (MQ=255)
                                                                                                             aaaaaGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAgc                                     >  3:320876/1‑149 (MQ=255)
                                                                                                                         cGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACt                         <  2:12295/149‑1 (MQ=255)
                                                                                                                                 gTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGt                 <  8:260777/149‑1 (MQ=255)
                                                                                                                                    aCCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACt                         >  2:28182/1‑138 (MQ=255)
                                                                                                                                    aCCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACt                         <  1:28182/138‑1 (MQ=255)
                                                                                                                                      cGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTa               >  4:295281/1‑146 (MQ=255)
                                                                                                                                       gCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGc                       >  2:183333/1‑137 (MQ=255)
                                                                                                                                       gCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGc                       <  1:183333/137‑1 (MQ=255)
                                                                                                                                       gCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTa               >  6:154282/1‑145 (MQ=255)
                                                                                                                                        cAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTTAAGAt          <  5:73914/149‑1 (MQ=255)
                                                                                                                                             cTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGtt     <  3:131286/149‑1 (MQ=255)
                                                                                                                                             cTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGtt     >  6:131813/1‑149 (MQ=255)
                                                                                                                                                ggTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGa  <  6:292305/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGA  >  NC_000913/2048172‑2048464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑