Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	909,353	G→A	A566V (GCG→GTG)	poxB ←	pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	909,353	0	G	A	100.0%	191.7 / NA	57	A566V (GCG→GTG)	poxB	pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/29);  total (28/29)

CGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGT  >  NC_000913/909210‑909497                                                                                                                                                |                                                                                                                                                 cgtcgtCCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGt                                                                                                                                             <  4:110255/149‑1 (MQ=255) cgtcgtCACCAAACGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCATTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGGTTGTTTTCACCAGt                                                                                                                                             >  8:347336/1‑149 (MQ=255)  gtcgtcCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGtt                                                                                                                                            <  4:28295/149‑1 (MQ=255)   tcgtcCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTc                                                                                                                                           <  7:153279/149‑1 (MQ=255)   tcgtcCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTc                                                                                                                                           >  8:278124/1‑149 (MQ=255)     gtcCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGa                                                                                                                                         >  6:114567/1‑149 (MQ=255)                    ggcCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACcgc                                                                                                                          <  1:303478/149‑1 (MQ=255)                      ccATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGt                                                                                                                        <  3:42385/149‑1 (MQ=255)                           atcGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGc                                                                                                                    <  7:278124/148‑1 (MQ=255)                             cGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGTGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTAGATCACTTCATCACCGCGTCCGCtga                                                                                                                 >  3:173945/1‑149 (MQ=255)                              gCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCtgat                                                                                                                <  5:69520/149‑1 (MQ=255)                               cTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCtgatg                                                                                                               <  3:230855/149‑1 (MQ=255)                                   gAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGAtt                                                                                                            <  3:121214/148‑1 (MQ=255)                                      cATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTgcgc                                                                                                        <  4:77270/149‑1 (MQ=255)                                                cTTGGTCGGGTAACGGTATCACTGCGTAAATCACTCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCAtata                                                                                              <  6:48191/149‑1 (MQ=255)                                                cTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCatat                                                                                               >  5:169938/1‑148 (MQ=255)                                                   ggTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATAc                                                                                             <  7:214662/147‑1 (MQ=255)                                                     tCGGGTAACGTTATCACTGCGTAAATCAATCATGGCATGTACTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGc                                                                                         <  6:275831/149‑1 (MQ=255)                                                        ggTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTg                                                                                       >  8:33480/1‑148 (MQ=255)                                                         gTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGaa                                                                                     >  1:355206/1‑149 (MQ=255)                                                             cGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAAcc                                                                                  <  6:142846/148‑1 (MQ=255)                                                             cGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAAcc                                                                                  >  5:142846/1‑148 (MQ=255)                                                               gTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCttt                                                                               <  5:114567/149‑1 (MQ=255)                                                                tATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTg                                                                              <  8:297482/149‑1 (MQ=255)                                                                tATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTg                                                                              <  2:355206/149‑1 (MQ=255)                                                                      tGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCttt                                                                               <  5:166024/142‑1 (MQ=255)                                                                      tGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCttt                                                                               >  6:166024/1‑142 (MQ=255)                                                                          tAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGa                                                                    <  7:76298/149‑1 (MQ=255)                                                                              tcaatcaTGGCATGTCCTTATTATGACGTGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGttt                                                                <  6:169938/149‑1 (MQ=255)                                                                              tcaatcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGttt                                                                <  8:95404/149‑1 (MQ=255)                                                                                aatcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGa                                                              >  5:61450/1‑149 (MQ=255)                                                                                  tcaTGGCATGTTCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATc                                                            <  1:83233/149‑1 (MQ=255)                                                                                  tcaTGGCATGTCCTTATTATGACGGGAAATGCCACCATTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGAATGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATc                                                            >  4:216979/1‑149 (MQ=255)                                                                                     tGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGc                                                         >  3:293076/1‑149 (MQ=255)                                                                                            tCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTAATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGaa                                                  >  5:10389/1‑149 (MQ=255)                                                                                              cTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAAt                                                 <  5:308591/148‑1 (MQ=255)                                                                                              cTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATg                                                >  7:287866/1‑149 (MQ=255)                                                                                               ttattaTGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATgg                                               >  8:222650/1‑149 (MQ=255)                                                                                                  ttatGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGGTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCt                                             >  2:35067/1‑148 (MQ=255)                                                                                                     tGAAGGGAAATGCCACCCTTTATACCTTAGCCAGTATGTTTTCACCAGATCGATCACTTCATCACAGATTCCGCTCATGATTGCGCGCAGCATATACAGGCTTAAACCTTTGGCCTGATGGAGTTTGATCTGCGGTGGAATGTCTaaat                                         >  4:40173/1‑147 (MQ=255)                                                                                                            aaaTGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCttt                                  <  8:287866/149‑1 (MQ=255)                                                                                                                  cACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGc                                              <  7:154168/131‑1 (MQ=255)                                                                                                                  cACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGc                                              >  8:154168/1‑131 (MQ=255)                                                                                                                   aCCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGt                                                      >  3:264280/1‑122 (MQ=255)                                                                                                                   aCCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGt                                                      <  4:264280/122‑1 (MQ=255)                                                                                                                   aCCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGacc                           <  7:33480/149‑1 (MQ=255)                                                                                                                     ccTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGaccac                         >  4:49756/1‑149 (MQ=255)                                                                                                                       tttttACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGAccacca                       >  1:175794/1‑149 (MQ=255)                                                                                                                       tttttACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCATTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGAccacca                       >  1:170694/1‑149 (MQ=255)                                                                                                                              cTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACAACAACATcac                 >  5:95225/1‑146 (MQ=255)                                                                                                                                   ccAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAaca           >  7:164325/1‑149 (MQ=255)                                                                                                                                     aGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACAcc         >  5:37344/1‑149 (MQ=255)                                                                                                                                      gtttgtttTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTc                                                                      <  5:161356/87‑1 (MQ=255)                                                                                                                                      gtttgtttTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTc                                                                      >  6:161356/1‑87 (MQ=255)                                                                                                                                       tttgtttTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCgg       >  7:132870/1‑149 (MQ=255)                                                                                                                                            tttCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGt  >  8:322695/1‑149 (MQ=255)                                                                                                                                             ttCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGt  <  7:322695/148‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                 CGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGT  >  NC_000913/909210‑909497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑