Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,725,445	G→A	5.6%	A63V (GCG→GTG)	ydhF ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,725,445	0	G	A	5.6%	226.6 / 2.1	71	A63V (GCG→GTG)	ydhF	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (37/30);  new base A (2/2);  total (39/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTTAATGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGACCAGCTGGCGGGCGGACATATTCCAGTCCATCAATCGCCAGTAGCCCATCACAAAACGGGA  >  NC_000913/1725308‑1725590                                                                                                                                          |                                                                                                                                                  tctTAATGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCAc                                                                                                                                        >  7:283320/1‑149 (MQ=255)  ctTAATGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACt                                                                                                                                       >  2:34253/1‑149 (MQ=255)     aaTGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGa                                                                                                                                     >  5:181394/1‑148 (MQ=255)     aaTGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGa                                                                                                                                     >  2:183815/1‑148 (MQ=255)      ttgatgTGATCGCGGTGTGTGATGTAATGACCATGGACTTTTTGTGCACGCGCGGTCGTCGCGATACCGAAGTTACAGCCGATTTCCATCCGTTAAGCCATGGGCGGTGCGAGTTGCAGTGCGTCGCCACACGCCGCTTCggagggg                                                                                                                                     >  6:366810/2‑141 (MQ=255)      atgatgTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATa                                                                                                                                   <  4:282997/149‑1 (MQ=255)      atgatgTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATa                                                                                                                                   <  3:322765/149‑1 (MQ=255)        gatgTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGc                                                                                                                                 >  5:247420/1‑149 (MQ=255)         atgTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGc                                                                                                                                 >  6:75163/1‑148 (MQ=255)          tgtgATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGcca                                                                                                                               >  8:195846/1‑149 (MQ=255)          tgtgATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGcca                                                                                                                               <  6:181394/149‑1 (MQ=255)              aTCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTTCCTCGCCAAACACCGCTTCGCACTGATAGccacca                                                                                                                            >  3:100796/1‑148 (MQ=255)                    gTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGccacca                                                                                                                            <  8:347425/142‑1 (MQ=255)                    gTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGccacca                                                                                                                            >  7:347425/1‑142 (MQ=255)                       aGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCa                                                                                                                   <  5:75163/148‑1 (MQ=255)                       aGTGATGTAATGACCAATGACGTGTTCTTCACGCGCGGTCGTCGCGTTACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCa                                                                                                                   <  2:68534/148‑1 (MQ=255)                          gATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCAt                                                                                                               >  2:276384/1‑149 (MQ=255)                           aTGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATg                                                                                                              <  7:195846/149‑1 (MQ=255)                                aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCa                                                                                                         >  1:275641/1‑149 (MQ=255)                                  gACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATg                                                                                                              >  1:315549/1‑142 (MQ=255)                                  gACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATg                                                                                                              <  2:315549/142‑1 (MQ=255)                                   aCCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACgg                                                                                                      <  6:247420/149‑1 (MQ=255)                                     cAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACggtg                                                                                                    <  3:9650/149‑1 (MQ=255)                                      aaTGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTATCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACggtgg                                                                                                   <  8:308595/149‑1 (MQ=255)                                                 cttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGcc                                                                                            >  5:103478/1‑145 (MQ=255)                                                  ttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGcc                                                                                            >  2:186725/1‑144 (MQ=255)                                                  ttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACACCGCTTCGCACTGCTAGCCACCATAAATATCAGCATGGTCCACggtg                                                                                                    >  2:195589/1‑136 (MQ=255)                                                  ttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACACCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACggtg                                                                                                    <  1:195589/136‑1 (MQ=255)                                                     aCGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATc                                                                                      >  5:317187/1‑147 (MQ=255)                                                     aCGCGCGATCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATaaa                                                                                                                        <  6:256382/113‑1 (MQ=255)                                                     aCGCGCGATCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATaaa                                                                                                                        >  5:256382/1‑113 (MQ=255)                                                       gcgcgGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATc                                                                                      >  6:163635/1‑145 (MQ=255)                                                       gcgcgGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGa                                                                                  <  6:317187/149‑1 (MQ=255)                                                          cgGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGc                                                                               <  5:163635/149‑1 (MQ=255)                                                            gtcgtcGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGctc                                                                             <  2:275641/149‑1 (MQ=255)                                                               gtcgCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCtt                                                                           >  8:79208/1‑148 (MQ=255)                                                                      tACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATaaaa                                                                   >  8:306180/1‑149 (MQ=255)                                                                       aCCGCATTTACTGACGATTTCCATCCTTTCAAGCATGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACACCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGGGGTCACGCCGAGATCCAGATGCTCTTCAATaaaa                                                                   <  4:100796/148‑1 (MQ=255)                                                                        ccGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACt                                                                 <  1:34253/149‑1 (MQ=255)                                                                         cGCATTTACTGACGATTTCCCTCCGTTCACGCAGGTGAGGTCCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCAAGGTCCACGGTGGTCACGCCGAGATCCAGATTCTCTTCAATAAAACt                                                                 >  4:214998/1‑148 (MQ=255)                                                                         cGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACt                                                                 >  7:161493/1‑148 (MQ=255)                                                                             tttACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGACCa                                                            <  1:276384/149‑1 (MQ=255)                                                                                  tGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGCTGCTCTTCAATAAAACTGACCAGCTgg                                                       >  2:263134/1‑149 (MQ=255)                                                                                      gATTTCCATCCGTTCACGCAGGTGAGGGGCCAGTTTCCGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGACCAGCTggcggg                                                   <  3:42048/149‑1 (MQ=255)                                                                                        tttCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGAc                                                              >  7:160554/1‑136 (MQ=255)                                                                                        tttCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGAc                                                              <  8:160554/136‑1 (MQ=255)                                                                                            cATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATg                                                                                <  7:25914/114‑1 (MQ=255)                                                                                            cATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATg                                                                                >  8:25914/1‑114 (MQ=255)                                                                                            cATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGACCAGCTGGCGGGCGGAc                                              <  7:306180/148‑1 (MQ=255)                                                                                            cATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGACCAGCTGGCGGGCGGACa                                             <  5:262943/149‑1 (MQ=255)                                                                                             aTCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGACCAGCTGGCGGGCGGACa                                             >  8:196632/1‑148 (MQ=255)                                                                                                 gTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGc                                                                                                                 <  1:357210/76‑1 (MQ=255)                                                                                                 gTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGc                                                                                                                 >  2:357210/1‑76 (MQ=255)                                                                                                 gTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGAcc                                                             >  6:305801/1‑128 (MQ=255)                                                                                                 gTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGAcc                                                             <  5:305801/128‑1 (MQ=255)                                                                                                 gTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGACCAGCTGGCGGGCGGACATATTc                                        <  6:103478/149‑1 (MQ=255)                                                                                                     aCGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGACCAGCTGGCGGGCGGACATATTCCa                                      <  7:250868/147‑1 (MQ=255)                                                                                                         aggtgaggtgCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGACCAGCTGGCGGGCGGACATATTCCAGTCCAt                                >  5:221404/1‑149 (MQ=255)                                                                                                             gaggtgCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGAc                                                              >  1:63304/1‑115 (MQ=255)                                                                                                             gaggtgCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGAc                                                              <  2:63304/115‑1 (MQ=255)                                                                                                              aggtgCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGACCAGCTGGCGGGCGGACATATTCCAGTCCATCAATc                           <  7:196632/149‑1 (MQ=255)                                                                                                                   ccAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGACCAGCTGGCGGGCGGACATATTCCAGTCCATCAATCGCCAg                      >  4:135766/1‑149 (MQ=255)                                                                                                                     aGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGACCAGCTGGCGGGCGGACATATTCCAGTCCATCAATCGCCAg                      >  6:274751/1‑147 (MQ=255)                                                                                                                         tCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAACACTGCCCAGCTGGCGGGCGGACATAGTCCCGTCCCTCACTCGCCAGTAGcc                 >  5:253962/1‑148 (MQ=255)                                                                                                                            gTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGACCAGCTGGCGGGCGGACATATTCCAGt                                    >  5:263983/1‑126 (MQ=255)                                                                                                                            gTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGACCAGCTGGCGGGCGGACATATTCCAGt                                    <  6:263983/126‑1 (MQ=255)                                                                                                                               ccTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGACCAGCTGGCGGGCGGACATATTCCAGTCCATCAATCGCCAGTAGCCCATcac           >  1:117465/1‑148 (MQ=255)                                                                                                                                 tCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGACCAGCTGGCGGGCGGACATATTCCAGTCCATCAATCGCCAGTAGCCCATCACaa         >  5:66289/1‑148 (MQ=255)                                                                                                                                     cAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGACCAGCTGGCGGGCGGACATATTCCAGTCCATCAATCTCCAGTAGCCCATCACAAAACgg    >  6:302981/1‑149 (MQ=255)                                                                                                                                       aaCGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGACCAGCTGGCGGGCGGACATATTCCAGTCCATCAATCGCCAGTAGCCCATCACAAAACggg   >  1:109479/1‑148 (MQ=255)                                                                                                                                       aaCGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGACCAGCTGGCGGGCGGACATATTCCAGTCCATCAATCGCCAGTAGCCCATCACAAAACGGGa  <  6:221404/149‑1 (MQ=255)                                                                                                                                          gccgcTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGAc                                                              >  7:358191/1‑86 (MQ=255)                                                                                                                                          gccgcTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGAc                                                              <  8:358191/86‑1 (MQ=255)                                                                                                                                          |                                                                                                                                                  TCTTAATGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGTTTCAGTGCCTCGCCAAACGCCGCTTCGCACTGATAGCCACCATAAATATCAGCATGGTCCACGGTGGTCACGCCGAGATCCAGATGCTCTTCAATAAAACTGACCAGCTGGCGGGCGGACATATTCCAGTCCATCAATCGCCAGTAGCCCATCACAAAACGGGA  >  NC_000913/1725308‑1725590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑