Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,145,560 A→G 5.3% V522V (GTA→GTG)  pflD → putative glycine radical domain‑containing pyruvate formate‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,145,5600AG 5.3% 231.4 / 3.2 75V522V (GTA→GTG) pflDputative glycine radical domain‑containing pyruvate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base A (35/36);  new base G (2/2);  total (37/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.08e-01

TCACCCTGATGGTTGAGGGCAGCAATATTTGTGATATCGGCCATCGCGACTGGGCACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTAAAAGCCAACTTCGCAACGCCAGAAGGCGAAAAAGTCCGCGCTCGC  >  NC_000913/4145412‑4145707
                                                                                                                                                    |                                                                                                                                                   
tCACCCTGATGGTTGAGGGCAGCAATATTTGTGATATCGGCCATCGCGACTGGGCACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTa                                                                                                                                                     >  6:301759/1‑149 (MQ=255)
   cccTGATGGTTGAGGGCAGCAGTATTTGTGATATCGGCCATCGCGACTGGGCACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAg                                                                                                                                                  <  8:350646/149‑1 (MQ=255)
           gTTGAGGGCAGCAATATTTGTGATATCGGCCATCGCGACTGGGCACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCg                                                                                                                                           <  3:154372/148‑1 (MQ=255)
             tGAGGGCAGCAATATTTGTGATATCGGCCATCGCGACTGGGCACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAgg                                                                                                                                                 >  5:126526/1‑140 (MQ=255)
             tGAGGGCAGCAATATTTGTGATATCGGCCATCGCGACTGGGCACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAgg                                                                                                                                                 <  6:126526/140‑1 (MQ=255)
               aGGGCAGCAATATTTGTGATATCGGCCATCGCGACTGGGCACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATc                                                                                                                                      <  5:143487/149‑1 (MQ=255)
                    agcaATATTTGTGATATCGGCCATCGCGACTGGGCACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACAGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCaa                                                                                                                                 >  5:232979/1‑149 (MQ=255)
                       aaTATTTGTGATATCGGCCATCGCGACTGGGCACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCt                                                                                                                              >  4:152565/1‑149 (MQ=255)
                       aaTATTTGTGATATCGGCCATCGCGACTGGGCACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCt                                                                                                                              <  1:292253/149‑1 (MQ=255)
                         tatTTGTGTTATCGGCCATCGCGACTGGGCACCTGTACCGCTGCTCTCCTCGGTTATCAGCGAGTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTAATAACTTCTCCGGGGTACAGGGGATCGGTAACGCCAACCt                                                                                                                              >  6:3765/1‑147 (MQ=255)
                           tttGTGATATCGGCCATCGCGACTGGGCACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGc                                                                                                                          >  6:5271/1‑149 (MQ=255)
                           tttGTGATATCGGCCATCGCGACTGGGCACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACAGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGc                                                                                                                          >  7:364511/1‑149 (MQ=255)
                                gATATCGGCCATCGCGCCTGGTCACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGCAAACAGGCCGCGATAATACCTATGGCGGCGCGCGGTATAACTTCTCCGGCGTAAAGGGAATCGATATCTCAAACCTGAGCGAtt                                                                                                                      >  4:48373/1‑148 (MQ=255)
                                           tCGCGACTGGGCACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGt                                                                                                          >  5:84221/1‑149 (MQ=255)
                                           tCGCGACTGGGCACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGt                                                                                                          <  5:5271/149‑1 (MQ=255)
                                           tCGCGACTGGGCACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGt                                                                                                          >  3:247210/1‑149 (MQ=255)
                                                  tGGGCACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAgg                                                                                                   >  2:124223/1‑149 (MQ=255)
                                                    ggCACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGGGTTATAACTTCTCCTGCGTACAGGGGATCGGTTTAGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAg                                                                                                    <  1:215192/146‑1 (MQ=255)
                                                    ggCACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGa                                                                                                 <  7:60325/149‑1 (MQ=255)
                                                     gCACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGAt                                                                                                <  6:232979/149‑1 (MQ=255)
                                                     gCACCTGTACCGCTGATCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGAt                                                                                                <  3:48373/149‑1 (MQ=255)
                                                      cACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATg                                                                                               <  6:84221/149‑1 (MQ=255)
                                                               cGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGc                                                                                                                                    >  8:106332/1‑103 (MQ=255)
                                                                  tgctCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCaa                                                                                   >  4:121802/1‑149 (MQ=255)
                                                                    ctctCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACa                                                                                 <  3:121802/149‑1 (MQ=255)
                                                                      ctcATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCACCAGc                                                                               >  8:91152/1‑149 (MQ=255)
                                                                       tcATCGTTTATCAGCTATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCg                                                                              >  8:167768/1‑149 (MQ=255)
                                                                       tcATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATTCGTTGAAAGGGATGGTTTTTGAGCAACAGCg                                                                              >  3:351637/1‑149 (MQ=255)
                                                                       tcATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCg                                                                              >  4:25005/1‑149 (MQ=255)
                                                                       tcATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCg                                                                              >  2:203715/1‑149 (MQ=255)
                                                                         aTCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGTTCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGtt                                                                            >  6:157079/1‑149 (MQ=255)
                                                                         aTCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGAt                                                                                                                                             >  7:37171/1‑84 (MQ=255)
                                                                         aTCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGAt                                                                                                                                             <  8:37171/84‑1 (MQ=255)
                                                                         aTCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACa                                                                                 <  7:19937/144‑1 (MQ=255)
                                                                         aTCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACa                                                                                 >  8:19937/1‑144 (MQ=255)
                                                                         aTCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGtt                                                                            >  7:258182/1‑149 (MQ=255)
                                                                         aTCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGtt                                                                            >  6:191712/1‑149 (MQ=255)
                                                                         aTCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGtt                                                                            >  6:116703/1‑149 (MQ=255)
                                                                            gTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTaa                                                                         <  4:113370/149‑1 (MQ=255)
                                                                             tttATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGAGGCGCGCGTTATAACTTCTCCGGAGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAg                                                                        >  6:370148/1‑149 (MQ=255)
                                                                                 tCAGCTATTGTCTGGAAAAAGGCCGCTATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGtttt                                                                    <  7:167768/149‑1 (MQ=255)
                                                                                 tCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGtttt                                                                    >  2:386360/1‑149 (MQ=255)
                                                                                   aGCGATTGTCTTGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGGTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCACGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGa                                                                  <  5:301759/149‑1 (MQ=255)
                                                                                   aGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTg                                                                   <  4:247210/148‑1 (MQ=255)
                                                                                    gCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTGCAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGa                                                                                                 >  8:27468/1‑117 (MQ=255)
                                                                                    gCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTGCAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGa                                                                                                 <  7:27468/117‑1 (MQ=255)
                                                                                    gCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGAtt                                                                                                                      <  8:42578/96‑1 (MQ=255)
                                                                                    gCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGAtt                                                                                                                      >  7:42578/1‑96 (MQ=255)
                                                                                               gAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGCGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTc                                                      >  3:58372/1‑149 (MQ=255)
                                                                                               gAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGc                                                                                     <  8:332907/118‑1 (MQ=255)
                                                                                               gAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGc                                                                                     >  7:332907/1‑118 (MQ=255)
                                                                                               gAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTaa                                                                         <  5:223673/130‑1 (MQ=255)
                                                                                               gAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTaa                                                                         >  6:223673/1‑130 (MQ=255)
                                                                                               gAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTc                                                      >  8:32502/1‑149 (MQ=255)
                                                                                                 aaaaGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCgg                                                    <  4:58372/149‑1 (MQ=255)
                                                                                                   aaGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTa                                                  <  1:124223/149‑1 (MQ=255)
                                                                                                         gcgATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTaaaa                                            <  1:203715/149‑1 (MQ=255)
                                                                                                         gcgATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTaaaa                                            <  5:116703/149‑1 (MQ=255)
                                                                                                             ttatACCGATGGCGGCGCGCGTTATTACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTCACCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTAAAAGCCa                                        <  2:76103/146‑1 (MQ=255)
                                                                                                                      tGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGaaa                                                                                                     <  3:314076/79‑1 (MQ=255)
                                                                                                                      tGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGaaa                                                                                                     >  4:314076/1‑79 (MQ=255)
                                                                                                                              cgcgTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTAAAAGCCAACTTCGCAACGCCAGaa                       <  1:386360/149‑1 (MQ=255)
                                                                                                                              cgcgTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTAAAAGCCAACTTCGCAACGCCAGaa                       <  3:152565/149‑1 (MQ=255)
                                                                                                                              cgcgTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGAATTAAAAGCCAACTTCGCAACGCCAGaa                       <  5:3765/149‑1 (MQ=255)
                                                                                                                                 gTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTAAAAGCCAACTTCGCAACGCCAGAAGGc                    >  3:156747/1‑149 (MQ=255)
                                                                                                                                  agccatcTTCTCCGGCGTACAGGTGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATTGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTAAAAGCCAACTTCGCAACGCCAGAAGGc                    <  5:222363/142‑1 (MQ=255)
                                                                                                                                   tataACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTAAAAGCCAACTTCGCAACGCCAGAAGGCg                   >  8:219756/1‑148 (MQ=255)
                                                                                                                                   tataACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTAAAAGCCAACTTCGCAACGCCAGAAGGCg                   >  8:40853/1‑148 (MQ=255)
                                                                                                                                   tataACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTAAAAGCCAACTTCGCAACGCCAGAAGGCg                   >  3:272714/1‑148 (MQ=255)
                                                                                                                                       aCTTCTCCGGCGTACAGGGGTTCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTAAAAGCCAACTTCGCAACGCCAGAAGGCGaaaaa              <  5:157079/149‑1 (MQ=255)
                                                                                                                                       aCTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTAAAAGCCAACTTCGCAACGCCAGAAGGCGaaaaa              <  4:217644/149‑1 (MQ=255)
                                                                                                                                         ttCTCCGGCGTGCAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGtt                                                                      >  2:38949/1‑91 (MQ=255)
                                                                                                                                         ttCTCCGGCGTGCAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGtt                                                                      <  1:38949/91‑1 (MQ=255)
                                                                                                                                              cGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTAAAAGCCAACTTCGCAACGCCAGAAGGCGAAAAAGTcc          <  7:32502/146‑1 (MQ=255)
                                                                                                                                                   tACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTAAAAGCCAACTTCGCAACGCCAGAAGGCGAAAAAGTCCGcgctcgc  <  8:258182/149‑1 (MQ=255)
                                                                                                                                                   tACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTAAAAGCCAACTTCGCAACGCCAGAAGGCGAAAAAGTCCGcgctcgc  <  5:370148/149‑1 (MQ=255)
                                                                                                                                                    aCAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTAAAAGCCAACTTCGCAACGCCAGAAgg                     >  2:27967/1‑129 (MQ=255)
                                                                                                                                                    aCAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTAAAAGCCAACTTCGCAACGCCAGAAgg                     <  1:27967/129‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCACCCTGATGGTTGAGGGCAGCAATATTTGTGATATCGGCCATCGCGACTGGGCACCTGTACCGCTGCTCTCATCGTTTATCAGCGATTGTCTGGAAAAAGGCCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTCTCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTAAAAGCCAACTTCGCAACGCCAGAAGGCGAAAAAGTCCGCGCTCGC  >  NC_000913/4145412‑4145707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑