Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,646,198 G→A 44.4% A272T (GCC→ACC)  rlmJ → 23S rRNA m(6)A2030 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,646,1980GA44.4% ‑1.5 / 5.8 9A272T (GCC→ACC) rlmJ23S rRNA m(6)A2030 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base A (2/2);  total (4/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCGCCGTGGAAACTGGAACAACAGATGAATAACGTGCTGCCGTGGCTGCACAGCAAACTGGTTCCGGCAGGCACCGGGCACGCCACCGTAAGCTGGATCGTGCCGGAGTAATTGCAGCCATTGCTGGCACCTATTACGTCTCGCGCTACAATCGCGGTAATCAACGATAAGGACACTTTGTCATGACTAAACACTATGA  >  NC_000913/3646116‑3646315
                                                                                  |                                                                                                                     
cccGCCGTGGAAACTGGAACAACAGATGAATAACGTGCTGCCGTGGCTGCACAGCAAACTGGTTCCGGCAGGCACCGGGCACGCCACCGTAAGCTGGATCGTGCCGGAGTAATTGCAGCCATTGCTGGCACCTATTACGTCTCGCGCTa                                                     >  5:345347/1‑149 (MQ=255)
     cgTGGAAACTGGAACAACAGATGAATAACGTGCTGCCGTGGCTGCACAGCAAACTGGTTCCGGCAGGCACCGGGCACGCCACCGTAAGCTGGATCGTGCCGGAGTAATTGCAGCCATTGCTGGCACCTATTACGTCTCGCGCTACAATc                                                <  1:24234/149‑1 (MQ=255)
        ggAAACTGGAACAACAGATGAATAACGTGCTGCCGTGGCTGCACAGCAAACTGGTTCCGGCAGGCACCGGGCACACCACCGTAAGCTGGATCGTGCCGGAGTAATTGCAGCCATTGCTGGCACCTATTACGTCTCGCGCTACAATcgcg                                             >  5:237358/1‑149 (MQ=255)
        ggAAACTGGAACAACAGATGAATAACGTGCTGCCGTGGCTGCACAGCAAACTGGTTCCGGCAGGCACCGGGCACACCACCGTAAGCTGGATCGTGCCGGAGTAATAGCAGCCATTGCTGGCACCTATTACGTCTCGCGCTACATTcgcg                                             >  5:237362/1‑149 (MQ=255)
                                                 cacaGCAAACTGGTTCCGGCAGGCACCGGGCACGCCACCGTAAGCTGGATCGTGCCGGAGTAATTGCAGCCATTGCTGGCACCTATTACGTCTCGCGCTACAATCGCGGTAATCAACGATAAGGACACTTTGTCATGACTAAACACTAt    <  6:345347/149‑1 (MQ=255)
                                                   gaggaaaCTGGTTCCGGCATGCACCGGGCACACCACCGTAAGCTGGATCGTGCCGGAGTAATTGCAGCCATTGCTGGCACCTATTACGTCTCGCGCTACAATCGCGGTAATCAACGATAAGGACACTTTGTCATGACTAATCACTATGa  <  6:237362/145‑1 (MQ=255)
                                                   caGCAAACTGGTTCCGGCAGGCACCGGGCACACCACCGTAAGCTGGATCGTGCCGGAGTAATTGCAGCCATTGCTGGCACCTATTACGTCTCGCGCTACAATCGCGGTAATCAACGATAAGGACACTTTGTCATGACTAAACACTATGa  <  6:237358/149‑1 (MQ=255)
                                                    aGCAAACTGGTTCCGGCAGGCACCGGGCACGCCACCGTAAGCTGGATCGTGCCGGAGTAATTGCAGCCATTGCTGGCACCTATTACGTCTCGCGCTACAATCGCGGTAATCAACGa                                  <  7:36495/116‑1 (MQ=255)
                                                    aGCAAACTGGTTCCGGCAGGCACCGGGCACGCCACCGTAAGCTGGATCGTGCCGGAGTAATTGCAGCCATTGCTGGCACCTATTACGTCTCGCGCTACAATCGCGGTAATCAACGa                                  >  8:36495/1‑116 (MQ=255)
                                                                                  |                                                                                                                     
CCCGCCGTGGAAACTGGAACAACAGATGAATAACGTGCTGCCGTGGCTGCACAGCAAACTGGTTCCGGCAGGCACCGGGCACGCCACCGTAAGCTGGATCGTGCCGGAGTAATTGCAGCCATTGCTGGCACCTATTACGTCTCGCGCTACAATCGCGGTAATCAACGATAAGGACACTTTGTCATGACTAAACACTATGA  >  NC_000913/3646116‑3646315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑