Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,128,066 G→A 5.0% intergenic (‑45/‑206) glcD ← / → glcC glycolate oxidase subunit, FAD‑linked/glycolate‑inducible glc operon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,128,0660GA 5.0% 257.3 / 2.3 79intergenic (‑45/‑206)glcD/glcCglycolate oxidase subunit, FAD‑linked/glycolate‑inducible glc operon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (37/38);  new base A (2/2);  total (39/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

TCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGT  >  NC_000913/3127924‑3128213
                                                                                                                                              |                                                                                                                                                     
tcAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCgctgc                                                                                                                                                 >  1:65115/1‑149 (MQ=255)
tcAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCgctgc                                                                                                                                                 >  7:23533/1‑149 (MQ=255)
    gTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCt                                                                                                                                             <  7:153769/149‑1 (MQ=255)
       cAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCtgtg                                                                                                                                          <  2:332346/149‑1 (MQ=255)
       cAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCtgt                                                                                                                                           <  6:170681/148‑1 (MQ=255)
          ggACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAAc                                                                                                                                       <  1:177828/149‑1 (MQ=255)
                    aCGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAg                                                                                                                             >  3:275942/1‑149 (MQ=255)
                       cAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCgg                                                                                                                          <  7:225595/149‑1 (MQ=255)
                             cATCAGTACCGATGTGCGGTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCtttt                                                                                                                    >  2:279587/1‑149 (MQ=255)
                             cATCAGTACCGATGTGCGGTCGACATCGGGTAAAGCGCCATCAAGACACTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCtttt                                                                                                                    >  8:117012/1‑149 (MQ=255)
                              aTCAGTACCGATGTGCGGTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCttttc                                                                                                                   <  8:135509/149‑1 (MQ=255)
                              aTCAGTACCGATGTGCGGTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCttttc                                                                                                                   >  7:135509/1‑149 (MQ=255)
                                 aGTACCGATGTGCGGTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCttttcgtt                                                                                                                <  8:23533/149‑1 (MQ=255)
                                   tACCGATGTGCGGGCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTGCGCTTTGTTGTGTGGTGTGGCAGCTGAGTTGTGCGCGCTGCTTCGGTGAACAGTTAGTAAGCGGGCgtgtcggtgt                                                                                                              >  5:254758/1‑145 (MQ=255)
                                     ccGATGTGCGGTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCttttcgttttc                                                                                                             <  3:183194/148‑1 (MQ=255)
                                       gATGTGCGGTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTc                                                                                                          >  1:18325/1‑149 (MQ=255)
                                              ggTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCtgt                                                                                                                                           <  2:186111/109‑1 (MQ=255)
                                              ggTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCtgt                                                                                                                                           >  1:186111/1‑109 (MQ=255)
                                              ggTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCttttcgttttc                                                                                                             <  7:87716/139‑1 (MQ=255)
                                              ggTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCttttcgttttc                                                                                                             >  8:87716/1‑139 (MQ=255)
                                               gTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCtgt                                                                                                                                           <  2:239315/108‑1 (MQ=255)
                                               gTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCtgt                                                                                                                                           >  1:239315/1‑108 (MQ=255)
                                               gTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCtt                                                                                                  >  3:336945/1‑149 (MQ=255)
                                                tCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCttt                                                                                                 <  8:173657/149‑1 (MQ=255)
                                                  gtcATCGGGTAATGCTCCATCTAGACGCTCTTCGTACAAGATGCTGATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCTGCTGTTTTTTTCGCGCTGCTTGTGTGAACTGTTATTATGCGGGCTTTTCGTTTTCGTCTATCTCTTTa                                                                                                <  4:275942/146‑1 (MQ=255)
                                                  gtcATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGTACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTa                                                                                                <  7:371434/146‑1 (MQ=255)
                                                  gaaaTCGTGTAACTCTCCTTCAATACGCTCTTCGTACAAGATGCTCATGAGTAGTCTTCGCTTTGTTGGTTTGGGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTATGCGGTCTTTTCGTTTTCGTCTATCTCTTTa                                                                                                <  6:254758/145‑1 (MQ=255)
                                                  gACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTa                                                                                                <  1:279587/148‑1 (MQ=255)
                                                  gACATCGGGTAAAGCGCCATCAAGACACTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTa                                                                                                <  7:117012/148‑1 (MQ=255)
                                                    cATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCttttcgttt                                                                                                               <  6:22225/131‑1 (MQ=255)
                                                    cATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCttttcgttt                                                                                                               >  5:22225/1‑131 (MQ=255)
                                                     aTCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCCGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTAtct                                                                                                     >  8:38986/1‑140 (MQ=255)
                                                     aTCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCCGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTAtct                                                                                                     <  7:38986/140‑1 (MQ=255)
                                                      tCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGaa                                                                                                                                        <  8:78276/104‑1 (MQ=255)
                                                      tCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGaa                                                                                                                                        >  7:78276/1‑104 (MQ=255)
                                                           tAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGt                                                                                       >  8:203842/1‑148 (MQ=255)
                                                             aaGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCa                                                                                     <  2:18325/148‑1 (MQ=255)
                                                                    aTCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAg                                                                                    >  1:379506/1‑142 (MQ=255)
                                                                    aTCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAg                                                                                    <  2:379506/142‑1 (MQ=255)
                                                                    aTCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGATTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGCCCA‑‑ttt                                                                           >  5:39431/1‑149 (MQ=255)
                                                                      cAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCACGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑ttttt                                                                         >  8:263978/1‑149 (MQ=255)
                                                                       aaGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑tttttt                                                                        >  7:324606/1‑149 (MQ=255)
                                                                            gCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTc                                                                     <  1:224674/147‑1 (MQ=255)
                                                                            gCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTc                                                                     >  2:224674/1‑147 (MQ=255)
                                                                              tctTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTa                                                                                                >  5:69883/1‑120 (MQ=255)
                                                                              tctTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTa                                                                                                <  6:69883/120‑1 (MQ=255)
                                                                                ttCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGctc                                                               >  3:231621/1‑149 (MQ=255)
                                                                                   gTAAAAGATGATAATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATGTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTATTCGATTTCGTCTATCTCTTTAGCTACCGGTCAGACAA‑‑ATTTTTATCCAGCTCtgt                                                            >  6:183487/1‑149 (MQ=255)
                                                                                    tACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTAACGGTCAGACCA‑‑TTTTTTTTCCAGCTAtgt                                                            >  4:215093/1‑148 (MQ=255)
                                                                                        aGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTc                                                                                                          >  4:91659/1‑100 (MQ=255)
                                                                                        aGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTc                                                                                                          <  3:91659/100‑1 (MQ=255)
                                                                                         gATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGCTCTGTGACCtt                                                      >  8:229709/1‑149 (MQ=255)
                                                                                           tGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGATGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTa                                                                                                <  1:371339/107‑1 (MQ=255)
                                                                                           tGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGATGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTa                                                                                                >  2:371339/1‑107 (MQ=255)
                                                                                             cTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGCTCTGTGACCTTGTCt                                                  >  3:231146/1‑149 (MQ=255)
                                                                                              tCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGCCCA‑‑TTTTTTTTCCAGCTGTGGGACCTTGTcgt                                                 >  2:154595/1‑147 (MQ=255)
                                                                                                        gCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTc                                                                     >  6:48151/1‑119 (MQ=255)
                                                                                                        gCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTc                                                                     <  5:48151/119‑1 (MQ=255)
                                                                                                          ttCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGGGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCa                                                                   <  7:355957/119‑1 (MQ=255)
                                                                                                          ttCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCa                                                                   >  8:355957/1‑119 (MQ=255)
                                                                                                            cGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATg                                    <  4:231621/148‑1 (MQ=255)
                                                                                                            cGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGt                                   >  3:234195/1‑149 (MQ=255)
                                                                                                             gCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGtt                                  <  2:65115/149‑1 (MQ=255)
                                                                                                              cTTTGTAGTGTTCTGTGGCAGCTGATTTTTGCGCGCTGCATCTGTGAACAGATAATAAGCGCGCTTTTCGTTTTCGTCTATCTCGTTACCTACAGGACAGACCA‑‑TTTTTATTCCAGCACTCTGACCTAGGATTGGATAACTCAATGtg                                  >  6:333832/1‑147 (MQ=255)
                                                                                                                 tgttgtgttgTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTaaa                               <  7:229709/148‑1 (MQ=255)
                                                                                                                  gttgtgttgtGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAAt                              <  4:231146/148‑1 (MQ=255)
                                                                                                                   ttgtgttgtgTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTg                            <  7:164855/149‑1 (MQ=255)
                                                                                                                      tgttgtgtGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGCTCtgt                                                            >  4:120612/1‑114 (MQ=255)
                                                                                                                      tgttgtgtGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGCTCtgt                                                            <  3:120612/114‑1 (MQ=255)
                                                                                                                        ttgtgtGGCAGCTGATTTTTGCACGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGt                        <  7:263978/148‑1 (MQ=255)
                                                                                                                          gtgtgGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTaa                      <  4:234195/148‑1 (MQ=255)
                                                                                                                           tgtgGCAGCTGATTTTTGAGAGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCTTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAAc                     <  3:215093/148‑1 (MQ=255)
                                                                                                                               gCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAAt                >  4:270948/1‑149 (MQ=255)
                                                                                                                                 aGCTGATTTTTGCACGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATa                  >  7:51409/1‑145 (MQ=255)
                                                                                                                                 aGCTGATTTTTGCACGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATa                  <  8:51409/145‑1 (MQ=255)
                                                                                                                                  gCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCAc             <  4:336945/149‑1 (MQ=255)
                                                                                                                                  gCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCAc             >  3:184779/1‑149 (MQ=255)
                                                                                                                                    tGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCATTTTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATc               <  2:70267/147‑1 (MQ=255)
                                                                                                                                    tGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCATTTTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATc               >  1:70267/1‑147 (MQ=255)
                                                                                                                                             cgcgcTGCTTCTGTGAACAGTTATTAATCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGAtgt  <  1:154595/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                     
TCAAGTCCAGGGACATGCTCACGCAGTGCCATCAGTACCGATGTGCGGTCGACATCGGGTAAAGCGCCATCAAGACGCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑‑TTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGT  >  NC_000913/3127924‑3128213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: