Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,653 C→A 100% L28F (TTG→TTT ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,6530CA86.5% 247.2 / 39.0 96coding (84/480 nt)ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (3/4);  major base A (34/42);  minor base . (6/7);  total (43/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.20e-01
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 104 total aligned reads displayed.

ATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTCCC  >  NC_000913/238508‑238798
                                                                                                                                                   |                                                                                                                                                 
atgttattaTCGTAAGTAAAATTACATGAATATTTAAAAAGGAATACGACATGAAACCGAAGCACAGAACCACCATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCTGTaaaaaaaaaa                                                                                                                                                <  4:111918/146‑1 (MQ=255)
aTTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAaaaaaaaa                                                                                                                                                 <  2:264873/148‑1 (MQ=255)
 ttttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAaag                                                                                                                                               <  1:216606/149‑1 (MQ=255)
    tattatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAaagaa                                                                                                                                             <  2:57673/148‑1 (MQ=255)
      ttatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAA‑‑AAAGAAGCAtt                                                                                                                                        <  7:294454/149‑1 (MQ=255)
        atCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCAt                                                                                                                                         <  3:302936/148‑1 (MQ=255)
         tCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGTGAAAAAAAAAATAAACAtt                                                                                                                                        >  8:150566/1‑148 (MQ=255)
         tCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt                                                                                                                                        >  8:33470/1‑148 (MQ=255)
         tCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCAtt                                                                                                                                        <  4:330087/148‑1 (MQ=255)
         tCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAGAAGCATTg                                                                                                                                       >  2:318592/1‑148 (MQ=255)
         tCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACACAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCAtt                                                                                                                                        <  6:113533/148‑1 (MQ=255)
            taagtaaAATTACATGAATATTTAAAAAGGAAAACGACATTAAACCGAAGCACATAATCAACATTCTCCAATCATAAAATATTTCCGTGTAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTAAAt                                                                                                                                    >  2:372795/1‑149 (MQ=255)
                taaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaaaaaa                                                                                                                                                >  1:287350/1‑133 (MQ=255)
                taaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaaaaaa                                                                                                                                                <  2:287350/133‑1 (MQ=255)
                   aaTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAggg                                                                                                                              >  8:330169/1‑148 (MQ=255)
                         aTGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGaaaaa                                                                                                                         <  6:177250/147‑1 (MQ=255)
                         aTGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGaaaaa                                                                                                                         >  5:177250/1‑147 (MQ=255)
                         aTGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCAGGTAAAAAAAAAGAAGCATTGAATGCAGGGAAAaataa                                                                                                                      >  1:272099/1‑149 (MQ=255)
                           gAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCAtt                                                                                                                                        >  6:149445/1‑130 (MQ=255)
                           gAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCAtt                                                                                                                                        <  5:149445/130‑1 (MQ=255)
                           gAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAaataa                                                                                                                      >  6:10979/1‑148 (MQ=255)
                                    aaaaGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAATAAGCATTGAATGCAGGGAAAAATAATATGGACATa                                                                                                            >  2:86287/1‑148 (MQ=255)
                                     aaaGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATAGAATGCAGGGAAAAATAATATGGCCATa                                                                                                            >  8:101754/1‑148 (MQ=255)
                                        tgAAAACTACATGAAACCGAAGCACATAATAAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGTGGTTTAACTCCGGTAAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATaaaa                                                                                                         <  8:168093/148‑1 (MQ=255)
                                        ggAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAg                                                                                                                                >  3:137531/1‑125 (MQ=255)
                                        ggAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAg                                                                                                                                <  4:137531/125‑1 (MQ=255)
                                         gAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAAc                                                                                                      <  1:318592/149‑1 (MQ=255)
                                            aaCGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAATACCATTTTATTGAAGGTAAAATTAATAGGGCAATaaaaacaag                                                                                                    >  4:63156/1‑145 (MQ=255)
                                             aCGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATc                                                                                                   <  7:189899/149‑1 (MQ=255)
                                                 cATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATATAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCgaaa                                                                                               <  3:328812/149‑1 (MQ=255)
                                                 cATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCgaaa                                                                                               <  5:80457/149‑1 (MQ=255)
                                                    gAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTACAGAGGAGAATTTTATTATTGAATATAGAGTTTTAACTACGGTAAAAAAAAAAGAAGCATTGAAAGCAGGTAAAAATAAAATGGCCATAAAAAACAACtaaa                                                                                               >  4:65564/1‑146 (MQ=255)
                                                    gAAAACGAAGCAAAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGATGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCgaaa                                                                                               <  3:65564/146‑1 (MQ=255)
                                                     aaaCCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGAAGGGAAAAATAATATGGCCATAAAAAACATCgaaagaa                                                                                            >  5:64827/1‑148 (MQ=255)
                                                      aaCCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAAc                                                                                          <  3:101583/149‑1 (MQ=255)
                                                         cGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGc                                                                                                                >  8:227988/1‑124 (MQ=255)
                                                         cGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGc                                                                                                                <  7:227988/124‑1 (MQ=255)
                                                          gAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAAAAATATGGCCATAAAAAAAAACTAAACAAActct                                                                                       >  3:27887/1‑149 (MQ=255)
                                                             gCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATgg                                                                                                                 <  4:134205/119‑1 (MQ=255)
                                                               acaGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTtttaat                                                                                 <  7:33470/149‑1 (MQ=255)
                                                               acaGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTtttaat                                                                                 <  4:336383/149‑1 (MQ=255)
                                                                             ctcCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAAc                                                                    <  6:368908/148‑1 (MQ=255)
                                                                              tcCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAatat                                                                                                                   <  7:72080/100‑1 (MQ=255)
                                                                              tcCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACg                                                                   >  8:3439/1‑148 (MQ=255)
                                                                              tcCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACg                                                                   >  3:191954/1‑148 (MQ=255)
                                                                              tcCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACg                                                                   >  7:247211/1‑148 (MQ=255)
                                                                               ccAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCa                                                                 >  8:180030/1‑149 (MQ=255)
                                                                                cAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGaaa                                                                                                                           >  2:55537/1‑90 (MQ=255)
                                                                                cAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGaaa                                                                                                                           <  1:55537/90‑1 (MQ=255)
                                                                                cAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTtttaattta                                                                              >  6:278659/1‑135 (MQ=255)
                                                                                cAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTtttaattta                                                                              <  5:278659/135‑1 (MQ=255)
                                                                                cAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCAt                                                                <  7:224354/149‑1 (MQ=255)
                                                                                 aaTCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATg                                                                                                                                   >  8:106756/1‑81 (MQ=255)
                                                                                 aaTCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATg                                                                                                                                   <  7:106756/81‑1 (MQ=255)
                                                                                 aaTCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCg                                                                                                  >  2:214858/1‑114 (MQ=255)
                                                                                 aaTCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCg                                                                                                  <  1:214858/114‑1 (MQ=255)
                                                                                   tCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGt                                                             <  7:330169/149‑1 (MQ=255)
                                                                                   tCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATgg                                                              <  7:53651/148‑1 (MQ=255)
                                                                                   tCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATgg                                                              >  8:53651/1‑148 (MQ=255)
                                                                                     aTAAAATATTTCCGTGGACCATTTTATTCTTGAATATAGAGGGTTAAATCCGGTAAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGt                                                             <  4:27887/148‑1 (MQ=255)
                                                                                      tAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCAATAAAAAACATCTAAAGAAACTCTttta                                                                                   >  2:98841/1‑124 (MQ=255)
                                                                                      tAAAATATTTCAGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTaa                                                          <  5:134894/149‑1 (MQ=255)
                                                                                       aaaaTATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGACATAAAAAAAATCGAAAGAAACTATTTTAATTTAAAATGTAAACTCATGGTTaa                                                          >  5:361564/1‑148 (MQ=255)
                                                                                        aaaTAATTCCGTGTAGCATTTTATTATTTAATATAGAGGTTTAAATCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAAt                                                         <  6:361564/148‑1 (MQ=255)
                                                                                             tttCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaaaaaa                                                                                                                                                >  1:307977/1‑56 (MQ=255)
                                                                                             tttCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaaaaaa                                                                                                                                                <  2:307977/56‑1 (MQ=255)
                                                                                             tttCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCat                                                   <  8:247211/149‑1 (MQ=255)
                                                                                                 cGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATc                                                                                                   >  7:272303/1‑96 (MQ=255)
                                                                                                 cGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATc                                                                                                   <  8:272303/96‑1 (MQ=255)
                                                                                                     gAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACgg                                            <  5:283899/149‑1 (MQ=255)
                                                                                                       gCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTaa                                                                      >  4:248299/1‑120 (MQ=255)
                                                                                                       gCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTaa                                                                      <  3:248299/120‑1 (MQ=255)
                                                                                                         aTTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAAGAAGCATTGAATGCAGGTAAAAATAATATGGCCATAAAAAACATCGAAATAAACTATTTTAATTTAACATGTAAACGCATGGTTAATAATCATAAAACGGTTg                                         >  5:30379/1‑148 (MQ=255)
                                                                                                          ttttATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACgg                                            <  8:22897/142‑1 (MQ=255)
                                                                                                             tattatTGAATATAGAGTTTTAACTCCGTTAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAgtgt                                   <  1:86287/148‑1 (MQ=255)
                                                                                                              attattGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAgtgt                                   <  6:64827/148‑1 (MQ=255)
                                                                                                               ttattGAATATAGAGGTTTTACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGtt                                  <  2:115353/148‑1 (MQ=255)
                                                                                                                 attTAATATATAGTTTTAAATCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAATCGCATGGTTAATCCTCATATCACGGGTGGAGTGTTaa                                <  1:372795/148‑1 (MQ=255)
                                                                                                                 attGAATATAGAGTTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATTCAGTGAAAAATAATATGTCCATAAAAAACATCGAAAGAAACTATTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTaa                                <  7:150566/148‑1 (MQ=255)
                                                                                                                  ttGTATTTAGAGGTTTAACTCCGTTTAAAAAAAAAAGAATCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTaa                                <  6:30379/148‑1 (MQ=255)
                                                                                                                  ttGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAgtgt                                   >  4:223560/1‑144 (MQ=255)
                                                                                                                  ttGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAgtgt                                   <  3:223560/144‑1 (MQ=255)
                                                                                                                  ttGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAg                               >  1:219379/1‑148 (MQ=255)
                                                                                                                  ttGAATATAGAGGTTTAACTCCGGTAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGaa                             <  8:120293/149‑1 (MQ=255)
                                                                                                                  ttGAATATAGAGGTTTAACTCCGGTAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGa                              >  3:960/1‑148 (MQ=255)
                                                                                                                   tGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCt                                                      <  8:218443/124‑1 (MQ=255)
                                                                                                                   tGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAAGTTAACATGTAAACGCATGGTTAATCCt                                                      >  7:218443/1‑124 (MQ=255)
                                                                                                                    gAATATAGAGGTTTAACTCCGGTAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAac                            <  4:960/148‑1 (MQ=255)
                                                                                                                               tttAAATTCGGTAAAAAAAAAAGAAGCATTGAATGCATGGAAAAATAATATGTCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAAt                  <  3:63156/148‑1 (MQ=255)
                                                                                                                                  aaCTCCGGTAAAAAAAAAAGAAGCATTGAATGCATGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGa               <  7:3439/148‑1 (MQ=255)
                                                                                                                                  aaCTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGa               <  7:180030/148‑1 (MQ=255)
                                                                                                                                     tacGGTAAAAAAAAAAGAAACATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATACTCATACCACGGGTGGAGAGTTAAGAAGATACATAAATGGCGtga           >  8:349148/3‑147 (MQ=255)
                                                                                                                                     tCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCa           >  7:344006/1‑149 (MQ=255)
                                                                                                                                     tCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATACTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCa           >  3:278464/1‑149 (MQ=255)
                                                                                                                                       cGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATg         >  4:339262/1‑149 (MQ=255)
                                                                                                                                        ggTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGt        >  3:166381/1‑149 (MQ=255)
                                                                                                                                          gTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTaa                                <  4:120682/124‑1 (MQ=255)
                                                                                                                                              aaaaaaaaAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTc    >  3:116685/1‑149 (MQ=255)
                                                                                                                                                aaaaaaAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATATATGGAGTGATGGTTTccc  >  2:74350/1‑149 (MQ=255)
                                                                                                                                                aaaCAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTccc  <  4:141183/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTCCC  >  NC_000913/238508‑238798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: