Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,305,264 | C→T | 6.5% | K497K (AAG→AAA) | mqo ← | malate dehydrogenase, FAD/NAD(P)‑binding domain |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,305,264 | 0 | C | T | 6.5% | 191.7 / 2.7 | 61 | K497K (AAG→AAA) | mqo | malate dehydrogenase, FAD/NAD(P)‑binding domain |
Reads supporting (aligned to +/- strand): ref base C (31/26); new base T (2/2); total (33/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.14e-01 |
ATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATG > NC_000913/2305118‑2305411 | atatCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCtt < 3:153674/149‑1 (MQ=255) ccACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCa > 5:141694/1‑149 (MQ=255) cACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCAt < 7:282747/149‑1 (MQ=255) cttcttTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCAt > 7:346105/1‑147 (MQ=255) ttcttTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCAt > 4:165751/1‑146 (MQ=255) ttcttTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCAt > 3:28744/1‑146 (MQ=255) cttTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGAc > 5:272707/1‑149 (MQ=255) aCGGGTTGCGGTTTCAACTGCGGGTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCCGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGAt < 8:291880/149‑1 (MQ=255) cGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTTCTTCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATc < 2:94942/149‑1 (MQ=255) cGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTc < 3:165751/148‑1 (MQ=255) gTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAAc < 6:272707/149‑1 (MQ=255) cAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTa < 1:234977/148‑1 (MQ=255) tttCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCAtt > 2:193015/1‑149 (MQ=255) gTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCt > 8:297061/1‑149 (MQ=255) tCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAGCGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGaatg > 1:53751/1‑147 (MQ=255) tgctgcCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGa < 6:179546/110‑1 (MQ=255) tgctgcCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGa > 5:179546/1‑110 (MQ=255) ctgcctgcGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAac < 6:141694/145‑1 (MQ=255) tgcctgcGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGAt > 8:246764/1‑149 (MQ=255) cctgcGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATc < 4:28744/148‑1 (MQ=255) gTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACtt > 1:188777/1‑149 (MQ=255) gTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACt < 7:297061/148‑1 (MQ=255) tCGTAGTTCAACCCCAGCACTTCGCTGTTGTACTGCAACTCGCGTTCTTTTGCCGCTACATCCCCGATCAGCTTGCGTCCATAAGACTGAACGATCGCTTTCACCGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAAAACttt > 3:274485/1‑149 (MQ=255) gTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACttttt < 8:346105/149‑1 (MQ=255) tAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTc > 1:30038/1‑149 (MQ=255) tAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTc < 2:188777/149‑1 (MQ=255) aGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGc < 5:147262/135‑1 (MQ=255) aGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGc > 6:147262/1‑135 (MQ=255) ttCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACAACACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCcag > 5:335827/1‑149 (MQ=255) aaCCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCGACATCACCGTTCGGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCAGTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCcagcag > 5:285327/1‑149 (MQ=255) ccAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGAtt > 3:112400/1‑148 (MQ=255) cAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTc > 4:251841/1‑148 (MQ=255) aGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCaa < 3:251841/149‑1 (MQ=255) gCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAAc < 4:112400/149‑1 (MQ=255) ttCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCTGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGAt < 1:178519/140‑1 (MQ=255) ttCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGAt > 2:178519/1‑140 (MQ=255) ttCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATaa > 6:272693/1‑149 (MQ=255) cTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCACCGTAGCATGCAATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATcgg > 6:99988/1‑149 (MQ=255) aCTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACtttt > 5:227889/1‑113 (MQ=255) aCTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACtttt < 6:227889/113‑1 (MQ=255) tCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACAt < 4:91328/128‑1 (MQ=255) tCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACAt > 3:91328/1‑128 (MQ=255) tCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGc > 6:299322/1‑149 (MQ=255) tCGCGATCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAATCACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGc > 1:130395/1‑149 (MQ=255) tCTGTTGCCGCTACATCACCGTTCAGTTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTgg > 7:239006/1‑149 (MQ=255) tCTGTTGCCGCTACATCACCGTTCAGTTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTgg > 7:244918/1‑149 (MQ=255) tGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATc > 8:325885/1‑49 (MQ=255) tGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATc < 7:325885/49‑1 (MQ=255) tGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGc > 2:77325/1‑148 (MQ=255) ccGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGcc > 8:8026/1‑149 (MQ=255) ccGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGc < 2:30038/148‑1 (MQ=255) aTCACCGTTCAGTTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGa < 8:239006/149‑1 (MQ=255) aTCACCGTTCAGTTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGa < 8:244918/149‑1 (MQ=255) tCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGcccc > 3:299741/1‑143 (MQ=255) tCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGcccc < 4:299741/143‑1 (MQ=255) cGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGcgcg > 2:175216/1‑149 (MQ=255) cGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGcgcg < 5:299322/149‑1 (MQ=255) gTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCgg < 6:335827/149‑1 (MQ=255) aGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAAt > 6:286141/1‑149 (MQ=255) gCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATg < 1:193015/149‑1 (MQ=255) gCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATg < 5:286141/149‑1 (MQ=255) | ATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATG > NC_000913/2305118‑2305411 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |