Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,945,057 C→T 8.3% M100I (ATG→ATA)  ruvB ← ATP‑dependent DNA helicase, component of RuvABC resolvasome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,945,0570CT 8.3% 142.2 / 2.9 48M100I (ATG→ATA) ruvBATP‑dependent DNA helicase, component of RuvABC resolvasome
Reads supporting (aligned to +/- strand):  ref base C (21/23);  new base T (2/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.76e-01

GCCGGACCTTCACCAATCATGATATCCAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGG  >  NC_000913/1944926‑1945195
                                                                                                                                   |                                                                                                                                          
gccgGACCTTCACCAATCATGATATCCAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATcgcc                                                                                                                           >  4:165480/1‑149 (MQ=255)
 ccgGACCTTCACCAATCATGATATCCAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATcgccc                                                                                                                          >  8:217716/1‑149 (MQ=255)
    gACCTTCACCAATCATGATATCCAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATcgcccgcc                                                                                                                       >  6:178330/1‑149 (MQ=255)
       cTTCACCAATCATGATATCCAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCCTGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCttt                                                                                                                    >  6:150137/1‑149 (MQ=255)
                tCATGATATCCAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATTCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCAcc                                                                                                           >  6:213150/1‑149 (MQ=255)
                  aTGATATCCAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCg                                                                                                          >  7:164860/1‑148 (MQ=255)
                     atatCCAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGAc                                                                                                       <  5:6649/148‑1 (MQ=255)
                            gTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTc                                                                                               <  8:342114/149‑1 (MQ=255)
                               ggTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGt                                                                                             <  5:133413/148‑1 (MQ=255)
                                gTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCAc                                                                                                            >  8:1883/1‑132 (MQ=255)
                                gTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCAc                                                                                                            <  7:1883/132‑1 (MQ=255)
                                gTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGc                                                                                           >  2:969/1‑149 (MQ=255)
                                gTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGc                                                                                           <  7:109262/149‑1 (MQ=255)
                                gTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGc                                                                                           <  1:969/149‑1 (MQ=255)
                                       tCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAAt                                                                                     >  1:325754/1‑148 (MQ=255)
                                          aTTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTc                                                                                                                  <  2:227399/116‑1 (MQ=255)
                                          aTTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTc                                                                                                                  >  1:227399/1‑116 (MQ=255)
                                          aTTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAAc                                                                                 >  7:134349/1‑149 (MQ=255)
                                          aTTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAAc                                                                                 <  7:112101/149‑1 (MQ=255)
                                             gCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGc                                                                               <  8:164860/148‑1 (MQ=255)
                                                      aGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGTATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATAAACGCCCATTTCAtt                                                                     >  8:206418/1‑149 (MQ=255)
                                                      aGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGTATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATAAACGCCCATTTAAtt                                                                     >  8:200907/1‑149 (MQ=255)
                                                      aGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCAtt                                                                     >  5:23144/1‑149 (MQ=255)
                                                      aGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCAtt                                                                     >  5:37302/1‑149 (MQ=255)
                                                      aGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCAtt                                                                     <  5:178330/149‑1 (MQ=255)
                                                       gCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTg                                                                    <  8:134349/149‑1 (MQ=255)
                                                       gCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTg                                                                    <  3:211456/149‑1 (MQ=255)
                                                        cACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTgg                                                                   <  3:165480/149‑1 (MQ=255)
                                                         aCTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGc                                                                  >  7:34390/1‑149 (MQ=255)
                                                                    aacTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTgg                                                       >  2:257706/1‑149 (MQ=255)
                                                                     acTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATATCATTGGCGACGATGGTGGc                                                      <  2:166405/149‑1 (MQ=255)
                                                                         gCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGc                                                  <  7:217716/149‑1 (MQ=255)
                                                                               gACGGTGGATCTCATAAATAAACAGCACGTCATGCGGTTCAAGGTTAGGGAGCCTCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGt                                             <  5:150137/148‑1 (MQ=255)
                                                                                        tctcATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAAc                                   >  6:220936/1‑149 (MQ=255)
                                                                                            atcaATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCaa                                    <  2:139364/144‑1 (MQ=255)
                                                                                            atcaATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCaa                                    >  1:139364/1‑144 (MQ=255)
                                                                                               aaTAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCggag                            <  2:325754/149‑1 (MQ=255)
                                                                                               aaTAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCgga                             <  8:159189/148‑1 (MQ=255)
                                                                                               aaTAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCgga                             <  6:23144/148‑1 (MQ=255)
                                                                                               aaTAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCg                               >  7:159189/1‑146 (MQ=255)
                                                                                                aTAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCggag                            >  1:117296/1‑148 (MQ=255)
                                                                                                  aaaCAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGAc                         >  2:131481/1‑149 (MQ=255)
                                                                                                                 cGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAAt          <  1:131481/149‑1 (MQ=255)
                                                                                                                     tCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATc      >  4:340119/1‑149 (MQ=255)
                                                                                                                       aaGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCg     <  3:340119/148‑1 (MQ=255)
                                                                                                                        aGGTTAGTGAGTATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATAAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAg   <  7:206418/149‑1 (MQ=255)
                                                                                                                        aGGTTAGTGAGTATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATAAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAg   <  7:200907/149‑1 (MQ=255)
                                                                                                                         ggTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGTCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAgg  <  8:34390/149‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                          
GCCGGACCTTCACCAATCATGATATCCAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGG  >  NC_000913/1944926‑1945195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑