New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4389174 =	148 (1.880)	4 (0.050)	4/238	NT	2.8%	coding (197/3324 nt)	mscM	mechanosensitive channel protein, miniconductance
	?	NC_000913	4389211 =	137 (1.840)					coding (160/3324 nt)	mscM	mechanosensitive channel protein, miniconductance
Rejected: Frequency below/above cutoff threshold.

GGGGGGCGTACGCCGCGACGGCCCCCGATAGCAAACAAATCACTCAGGAACTGGAGCAGGCAAAAGCGGCGAAACCCGCACAGCCGGAAGTCGTAGAGGCGCTCCAGTCTGCCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCGCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4389327‑4389174 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑agcgcatTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGAT  >  NC_000913/4389211‑4389358                                                                                                                                                                                                                                                                                                                GGGGGGCGTACGCCGCGACGGCCCCCGATAGCAAACAAATCACTCAGGAACTGGAGCAGGCAAAAGCGGCGAAACCCGCACAGCCGGAAGTCGTAGAGGCGCTCCAGTCTGCCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAG                                                                                                                                                           >  4:188117/1‑149  GGGGGCGTACGCCGCGACGGCCCCCGATAGCAAACAAATCACTCAGGAACTGGAGCAGGCAAAAGCGGCGAAACCCGCACAGCCGGAAGTCGTAGAGGCGCTCCAGTCTGCCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGC                                                                                                                                                          <  3:99026/149‑1  GGGGGCGTACGCCGCGACGGCCCCCGATAGCAAACAAATCACTCAGGAACTGGAGCAGGCAAAAGCGGCGAAACCCGCACAGCCGGAAGTCGTAGAGGCGCTCCAGTCTGCCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAG                                                                                                                                                           <  1:62091/148‑1    GGGCGTACGCCGCGACGGCCCCCGATAGCAAACAAATCACTCAGGAACTGGAGCAGGCAAAAGCGGCGAAACCCGCACAGCCGGAAGTCGTAGAGGCGCTCCAGTCTGCCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCGC                                                                                                                                                        <  8:42921/149‑1    GGGCGTACGCCGCGACGGCCCCCGATAGCAAACAAATCACTCAGGAACTGGAGCAGGCAAAAGCGGCGAAACCCGCACAGCCGGAAGTCGTAGAGGCGCTCCAGTCTGCCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCG                                                                                                                                                         >  3:243201/1‑148     GGCGTACGCCGCGACGGCCCCCGATAGCAAACAAATCACTCAGGAACTGGAGCAGGCAAAAGCGGCGAAACCCGCACAGCCGGAAGTCGTAGAGGCGCTCCAGTCTGCCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCGCA                                                                                                                                                       <  3:156358/149‑1      GCGTACGCCGCGACGGCCCCCGATAGCAAACAAATCACTCAGGAACTGGAGCAGGCAAAAGCGGCGAAACCCGCACAGCCGGAAGTCGTAGAGGCGCTCCAGTCTGCCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCGCAT                                                                                                                                                      >  8:82248/1‑149                                     AAATCACTCAGGAACTGGAGCAGGCAAAAGCGGCGAAACCCGCACAGCCGGAAGTCGTAGAGGCGCTCCAGTCTGCCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGA                                                                                                                                                            >  6:176823/1‑112                                     AAATCACTCAGGAACTGGAGCAGGCAAAAGCGGCGAAACCCGCACAGCCGGAAGTCGTAGAGGCGCTCCAGTCTGCCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGA                                                                                                                                                            <  5:176823/112‑1                                                                                                                CCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGA                                                                            >  3:104362/1‑117                                                                                                                CCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGA                                                                            <  4:104362/117‑1                                                                                                                  TTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGT                                          >  3:327946/1‑149                                                                                                                                         AGGTTCCCTTGAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCAACTGAGGCACCAG                   <  4:327946/149‑1                                                                                                                                                    AGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCAACTGAGGCACCAGGCCATCAGAA         >  5:88700/1‑148                                                                                                                                                     GCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCAACTGAGGCACCAGGCCATCAGAAAA       <  1:312468/149‑1                                                                                                                                                     GCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCAACTGAGGCACCAGGCCATCAGAA         >  1:93779/1‑147                                                                                                                                                     GCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCAACTGA                           >  7:29801/1‑129                                                                                                                                                     GCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCAACTGA                           <  8:29801/129‑1                                                                                                                                                     GCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCGTTTGCCTGCTCCAGTTCCTGAGGGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCAACTGAGGCACCAGGCCATCAGAAAA       <  2:240688/149‑1                                                                                                                                                       GCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGT     <  3:302368/149‑1                                                                                                                                                         ATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGA   >  1:83284/1‑149                                                                                                                                                         ATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGA   <  8:136897/149‑1                                                                                                                                                          TTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGAT  <  3:224011/149‑1                                                                                                                                                          TTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGAT  <  2:245958/149‑1                                                                                                                                                          TTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGA   >  5:292341/1‑148                                                                                                                                                                                                                                                                                                                GGGGGGCGTACGCCGCGACGGCCCCCGATAGCAAACAAATCACTCAGGAACTGGAGCAGGCAAAAGCGGCGAAACCCGCACAGCCGGAAGTCGTAGAGGCGCTCCAGTCTGCCTTAAATGCGCTTGAGGAACGAAAAGGTTCCCTTGAGCGCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4389327‑4389174 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑agcgcatTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGAT  >  NC_000913/4389211‑4389358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.