| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 238,653 | C→A | L28F (TTG→TTT) | ykfM ← | lethality reduction protein, putative inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 238,653 | 0 | C | A | 87.5% | 19.7 / ‑2.3 | 8 | coding (84/480 nt) | ykfM | lethality reduction protein, putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (4/3); minor base . (1/0); total (5/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
TATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGT‑AAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTC > NC_000913/238512‑238796 | tattatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGT‑AAAAAAAAaag > 5:101176/1‑146 (MQ=255) tCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGT‑AAAAAAAAAAGAAGCAtt < 5:45191/148‑1 (MQ=255) tatTTAAAAACGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGT‑AAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATg < 2:76296/149‑1 (MQ=255) atTTAAAAAGGAAAACGACATGAACCCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGT‑AAAAAA‑AAAGAAGCATTGAATGCAGGGAAAAATAATATGGc > 6:23599/1‑149 (MQ=255) aaaCGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGT‑AAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAAc > 6:18048/1‑148 (MQ=255) ctcCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGT‑AAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAAc < 6:101176/148‑1 (MQ=255) gTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAATAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATTTAAACGCATTGTTAATCCAAATATc > 4:62502/1‑148 (MQ=255) aaCTCCGGT‑AAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGATAAGAACATACATAAATGGAg > 2:126971/1‑149 (MQ=255) aaaaaaaaAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTc < 5:18048/149‑1 (MQ=255) | TATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGT‑AAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTC > NC_000913/238512‑238796 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |