Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913114,5070AT21.1% 76.2 / 18.3 38intergenic (+20/+15)guaC/hofCGMP reductase/assembly protein in type IV pilin biogenesis, transmembrane protein
Reads supporting (aligned to +/- strand):  ref base A (15/15);  new base T (4/4);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ATATTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAG  >  NC_000913/114363‑114641
                                                                                                                                                |                                                                                                                                      
atatTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCaa                                                                                                                                    <  3:40834/149‑1 (MQ=255)
  atTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCaa                                                                                                                                    <  1:47343/147‑1 (MQ=255)
  atTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCaa                                                                                                                                    >  2:47343/1‑147 (MQ=255)
                         ttGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGTTGCTCCACGCCAGc                                                                                                                           >  1:193222/1‑133 (MQ=255)
                         ttGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGTTGCTCCACGCCAGc                                                                                                                           <  2:193222/133‑1 (MQ=255)
                               aTACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGTTGCTCCACGCCAGCGtt                                                                                                                        >  3:166782/1‑130 (MQ=255)
                               aTACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGTTGCTCCACGCCAGCGtt                                                                                                                        <  4:166782/130‑1 (MQ=255)
                                                  cTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAAtt                                                                                  >  6:49348/1‑149 (MQ=255)
                                                                      caccacGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGc                                                                               <  3:122900/132‑1 (MQ=255)
                                                                      caccacGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGc                                                                               >  4:122900/1‑132 (MQ=255)
                                                                                  tCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCaa                                                   >  2:202501/1‑148 (MQ=255)
                                                                                          aGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGc                                                                                                                               <  8:31576/64‑1 (MQ=255)
                                                                                          aGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGc                                                                                                                               >  7:31576/1‑64 (MQ=255)
                                                                                                  gAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCAAAAATGGAAAATTGTCAGATAAATTGCCACAACCAACGAACAAATAAATCCTCCCGTTAt                                   >  8:164166/1‑148 (MQ=255)
                                                                                                        cGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGc                            <  6:112736/149‑1 (MQ=255)
                                                                                                         gCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGtt                                                                                                                        <  2:208676/56‑1 (MQ=255)
                                                                                                         gCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGtt                                                                                                                        >  1:208676/1‑56 (MQ=255)
                                                                                                             cTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAg                             >  8:365/1‑143 (MQ=255)
                                                                                                             cTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAg                             <  7:365/143‑1 (MQ=255)
                                                                                                              ttCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAg                                                           <  1:56901/112‑1 (MQ=255)
                                                                                                              ttCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAg                                                           >  2:56901/1‑112 (MQ=255)
                                                                                                               tCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTgg                                                                                <  6:44303/90‑1 (MQ=255)
                                                                                                               tCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTgg                                                                                >  5:44303/1‑90 (MQ=255)
                                                                                                                 atcaacCTGTAATCTCCCAACGCTGGCTTGGAGCAACACGCCAGAGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGt                   <  7:164166/147‑1 (MQ=255)
                                                                                                                 aacaacCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTatccca                                                                                                                  >  1:32337/1‑54 (MQ=255)
                                                                                                                 aacaacCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTatccca                                                                                                                  <  2:32337/54‑1 (MQ=255)
                                                                                                                 aacaacCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGt                   >  2:40700/1‑149 (MQ=255)
                                                                                                                  acaacCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGtt                  >  4:130742/1‑149 (MQ=255)
                                                                                                                       cTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGt             <  5:49348/149‑1 (MQ=255)
                                                                                                                               tcCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTcatc                                                                                                       <  3:15197/51‑1 (MQ=255)
                                                                                                                               tcCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTcatc                                                                                                       >  4:15197/1‑51 (MQ=255)
                                                                                                                                gataaCGCTGGCGTGTTGCTCCACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAAc                         >  4:21150/4‑128 (MQ=39)
                                                                                                                                gataaCGCTGGCGTGTTGCTCCACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAAc                         <  3:21150/125‑1 (MQ=39)
                                                                                                                                  cAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAg  <  1:40700/149‑1 (MQ=255)
                                                                                                                                      gCTGGCGTGTTGCTCCACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATtcct                                           >  2:24170/1‑104 (MQ=39)
                                                                                                                                      gCTGGCGTGTTGCTCCACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATtcct                                           <  1:24170/104‑1 (MQ=39)
                                                                                                                                              ggAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCaa                                                   >  3:103639/1‑88 (MQ=255)
                                                                                                                                              ggAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCaa                                                   <  4:103639/88‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                      
ATATTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAG  >  NC_000913/114363‑114641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑