Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 909,422 G→A A543V (GCC→GTC)  poxB ← pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913909,4220GA98.7% 248.6 / NA 78A543V (GCC→GTC) poxBpyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (37/40);  minor base C (1/0);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCC  >  NC_000913/909283‑909567
                                                                                                                                           |                                                                                                                                                 
tAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGa                                                                                                                                          <  3:65913/149‑1 (MQ=255)
    tcaatcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGttt                                                                                                                                      >  6:54912/1‑149 (MQ=255)
      aatcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGa                                                                                                                                    >  3:274444/1‑149 (MQ=255)
       atcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGAt                                                                                                                                   <  1:217201/149‑1 (MQ=255)
       atcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGAt                                                                                                                                   <  1:371074/149‑1 (MQ=255)
              cATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCATCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGt                                                                                                                            <  1:108431/149‑1 (MQ=255)
               aTGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTg                                                                                                                           <  5:331803/149‑1 (MQ=255)
                    cTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATg                                                                                                                      >  1:190534/1‑149 (MQ=255)
                     ttattaTGACGGGAAATGTCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATgg                                                                                                                     >  4:99121/1‑149 (MQ=255)
                     ttattaTGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGtt                                                                                                                                             <  8:98833/125‑1 (MQ=255)
                     ttattaTGACGGGAAATGCCACCCTTTTTACCTTAGCCAGGTTGTTTTCGCCAGTTCGATCACTTCATCACAGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGtt                                                                                                                                             >  7:98833/1‑125 (MQ=255)
                          atGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGGCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTaa                                                                                                                 <  2:338577/148‑1 (MQ=255)
                             aCGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAActct                                                                                                             >  1:359199/1‑149 (MQ=255)
                               gggAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACtcttc                                                                                                           <  4:274444/149‑1 (MQ=255)
                               gggAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACtcttc                                                                                                           >  7:160154/1‑149 (MQ=255)
                               gggAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACtcttc                                                                                                           >  8:108629/1‑149 (MQ=255)
                                 gAAATGCCACCATTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTcttctt                                                                                                         <  5:375500/149‑1 (MQ=255)
                                  aaaTGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAGCCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCttt                                                                                                        >  6:31114/1‑149 (MQ=255)
                                        cACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATg                                                                                                                      <  8:358485/129‑1 (MQ=255)
                                        cACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATg                                                                                                                      >  7:358485/1‑129 (MQ=255)
                                         aCCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAACCCTTTGACCTGTTCGAGTTTGATCTGCGGGGGAATGGCTAACTCTTCTTTGGCGacc                                                                                                 <  3:201902/149‑1 (MQ=255)
                                           ccTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGGTTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGaccac                                                                                               >  8:101589/1‑149 (MQ=255)
                                            cTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGaccacc                                                                                              <  2:190534/149‑1 (MQ=255)
                                            cTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGaccacc                                                                                              <  3:99121/149‑1 (MQ=255)
                                               tttACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGAccacca                                                                                             <  1:354087/147‑1 (MQ=255)
                                                         ccAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAaca                                                                                 >  8:351318/1‑149 (MQ=255)
                                                          cAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCTGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACTAacac                                                                                <  6:110515/149‑1 (MQ=255)
                                                          cAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGc                                                                                                     >  2:401987/1‑128 (MQ=255)
                                                          cAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGc                                                                                                     <  1:401987/128‑1 (MQ=255)
                                                                 ttttCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCg                                                                         >  1:366102/1‑149 (MQ=255)
                                                                  tttCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGTTTATATTTTCTTTTTCGTCGACCACATCGACCAACACCGGACCGt                                                                        <  4:33395/149‑1 (MQ=255)
                                                                     cGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGac                                                                                                  >  8:354956/1‑120 (MQ=255)
                                                                     cGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGac                                                                                                  <  7:354956/120‑1 (MQ=255)
                                                                      gCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGGTTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGAt                                                                    <  7:101589/149‑1 (MQ=255)
                                                                      gCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGTCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGAt                                                                    <  2:366102/149‑1 (MQ=255)
                                                                      gCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACcaccac                                                                                            >  2:247121/1‑125 (MQ=255)
                                                                      gCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACcaccac                                                                                            <  1:247121/125‑1 (MQ=255)
                                                                      gCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGAt                                                                    >  8:111579/1‑149 (MQ=255)
                                                                       ccAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAACCCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCATCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGagg                                                                   >  2:316383/1‑147 (MQ=255)
                                                                       ccAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGa                                                                            <  6:328116/140‑1 (MQ=255)
                                                                       ccAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGa                                                                            >  5:328116/1‑140 (MQ=255)
                                                                          gTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATg                                                                   >  4:348784/1‑146 (MQ=255)
                                                                           ttCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATccacc                                                                                     <  6:9102/127‑1 (MQ=255)
                                                                           ttCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATccacc                                                                                     >  5:9102/1‑127 (MQ=255)
                                                                           ttCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAacac                                                                                <  8:234318/132‑1 (MQ=255)
                                                                           ttCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAacac                                                                                >  7:234318/1‑132 (MQ=255)
                                                                           ttCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACAACATCCACCAACACCGGACCGTCGATGgag                                                                >  5:83454/1‑148 (MQ=255)
                                                                             cGATCACTTCATCACCGCGGCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTGCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGaa                                                              <  1:10293/148‑1 (MQ=255)
                                                                              gATCACTTCATCACCTCGTCCGCTGATGATTGCTCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAgg                                                            >  2:8294/1‑149 (MQ=255)
                                                                              gATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAg                                                             <  7:108629/148‑1 (MQ=255)
                                                                                  aCTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGcgcg                                                        >  8:216247/1‑149 (MQ=255)
                                                                                   cTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGt                                                       >  1:353432/1‑149 (MQ=255)
                                                                                      catcaCCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGtt                                                      <  8:160154/147‑1 (MQ=255)
                                                                                           ccGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGc                                               >  1:103720/1‑149 (MQ=255)
                                                                                            cgcgTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGaccacc                                                                                              >  4:215261/1‑101 (MQ=255)
                                                                                              cgTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGCACTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGcta                                                                                                   >  1:302621/1‑92 (MQ=255)
                                                                                                    cTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAAc                                      <  5:54912/149‑1 (MQ=255)
                                                                                                        tgatTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTACCTCTTCTTTGGCGACCACCACATCCACCACCACCGGACCGGCGATGGAGAAGGCGCGTTCCAGGGCTTCATCAACTTc                                   >  2:249171/1‑148 (MQ=255)
                                                                                                          atTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTtcat                                          >  3:301027/1‑139 (MQ=255)
                                                                                                          atTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTtcat                                          <  4:301027/139‑1 (MQ=255)
                                                                                                               gcgcAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATccacc                                                                                     <  3:304112/91‑1 (MQ=255)
                                                                                                               gcgcAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATccacc                                                                                     >  4:304112/1‑91 (MQ=255)
                                                                                                                cgcAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGacc                                                                                                 >  1:212809/1‑78 (MQ=255)
                                                                                                                cgcAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGacc                                                                                                 <  2:212809/78‑1 (MQ=255)
                                                                                                                  cagcaTATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCttttt                        <  7:351318/149‑1 (MQ=255)
                                                                                                                   agcaTATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCTGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTc                       <  1:249171/149‑1 (MQ=255)
                                                                                                                   agcaTATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTc                       >  7:180153/1‑149 (MQ=255)
                                                                                                                     caTATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGGTGCAGGGCTTCATCAACTTCAGACGCTTTTTCt                      <  7:146399/148‑1 (MQ=255)
                                                                                                                     caTATACAGGCTGAAACCTTTGACCTGATCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGCGCTTCATCAACTACAGACGCTTTTTCt                      >  8:146399/1‑148 (MQ=255)
                                                                                                                      atatACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTac                    <  7:216247/149‑1 (MQ=255)
                                                                                                                       tataCAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCttttt                        >  7:311000/1‑144 (MQ=255)
                                                                                                                       tataCAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCttttt                        <  8:311000/144‑1 (MQ=255)
                                                                                                                        ataCAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACCTCCACCAACACCGGACCGTCGATGCAGAAGGCGCGTTGCAGGGCTGCATCAACTTCAGACGCTTTTTCTacac                  <  1:316383/149‑1 (MQ=255)
                                                                                                                         taCAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTacac                  <  7:111579/148‑1 (MQ=255)
                                                                                                                                tGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGACTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCCTTGCAGGGCTTCATCAACTTCAGCCGCTTTTTCTACACGGATAcca          >  2:404005/1‑148 (MQ=255)
                                                                                                                                  aaaCCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATAccc          <  1:8294/147‑1 (MQ=255)
                                                                                                                                    aCCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTaa      <  3:348784/149‑1 (MQ=255)
                                                                                                                                        ttGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGcc  >  2:39348/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                                 
TAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCC  >  NC_000913/909283‑909567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑