| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 167,843 | +T | coding (360/2244 nt) | fhuA → | ferrichrome outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 167,842 | 1 | . | T | 94.4% | 116.4 / 0.3 | 36 | coding (359/2244 nt) | fhuA | ferrichrome outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (15/19); total (16/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01 | |||||||||||
CCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTA‑TGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATA > NC_000913/167698‑167955 | ccGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTA‑ttga > 3:117898/1‑146 (MQ=255) aaaaaGTGCCACAGTCTATTTCTGTTGTGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTcg > 3:50210/1‑149 (MQ=255) aaaGTGCCACAGTCTATTTCTGTTGTGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTA‑tt > 7:22819/1‑129 (MQ=255) aaaGTGCCACAGTCTATTTCTGTTGTGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTA‑tt < 8:22819/130‑2 (MQ=255) caGTCTATTTCTGTTGTGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCgg < 2:1934/149‑1 (MQ=255) aGTCTATTTCTGTTGTGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGc < 5:129835/149‑1 (MQ=255) ttgtGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTATAAGAAGCGCTTAGCTACACGCCGGGTGTGTCTGTTCGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCaaa < 2:126634/148‑1 (MQ=255) tgACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCa < 4:50210/149‑1 (MQ=255) cgccgAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGaa > 6:15633/1‑148 (MQ=255) aaGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAgg > 4:95330/1‑131 (MQ=255) aaGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAgg < 3:95330/131‑1 (MQ=255) gagaTGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATc < 5:15633/149‑1 (MQ=255) agaTGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCt < 8:17221/149‑1 (MQ=255) gcgcTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGaccac > 3:90299/1‑94 (MQ=255) gcgcTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGaccac < 4:90299/94‑1 (MQ=255) cATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTg > 3:43347/1‑148 (MQ=255) aaGTCGGTAAAAGAAGCGCTTAGCTACATGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGc < 2:32545/99‑1 (MQ=255) aaGTCGGTAAAAGAAGCGCTTAGCTACATGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGc > 1:32545/1‑99 (MQ=255) ggTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAAc > 6:72829/1‑149 (MQ=255) tAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACtt < 1:113104/149‑1 (MQ=255) aagaagCGCTTAGCTACACGCCGGGTGGCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCCCTGCAACTTcag < 5:121367/149‑3 (MQ=255) agaagCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCAAACAACTATTTACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTGCta > 7:56043/1‑148 (MQ=255) aagCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATaa < 4:117898/149‑1 (MQ=255) tACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTca > 2:52700/1‑133 (MQ=255) tACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTca < 1:52700/134‑2 (MQ=255) acGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCAtt > 6:2815/1‑148 (MQ=255) cGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGAccc < 5:2815/149‑1 (MQ=255) tctcTGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGtata < 3:51456/148‑1 (MQ=255) tctGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGcc > 1:151323/1‑71 (MQ=255) tctGTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGcc < 2:151323/71‑1 (MQ=255) tctGTTGGTACGCGTGGCGCATCCAACACCTA‑TGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTAt < 4:29109/87‑1 (MQ=255) tctGTTGGTACGCGTGGCGCATCCAACACCTA‑TGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTAt > 3:29109/1‑87 (MQ=255) gTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCa > 7:77472/1‑75 (MQ=255) gTTGGTACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCa < 8:77472/75‑1 (MQ=255) tACGCGTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCta > 2:157770/1‑112 (MQ=255) cgTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCa < 6:138152/96‑1 (MQ=255) cgTGGCGCATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCa > 5:138152/1‑96 (MQ=255) gcgcATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGc > 6:129442/1‑91 (MQ=255) gcgcATCCAACACCTATTGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGc < 5:129442/91‑1 (MQ=255) | CCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTA‑TGACCACCTGATCATTCGCGGCTTTGCGGCAGAAGGCCAAAGCCAGAATAACTATCTGAATGGCCTGAAGTTGCAGGGCAACTTCTATAACGATGCGGTCATTGACCCGTATA > NC_000913/167698‑167955 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |