Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,683,585 G→A 39.6% intergenic (‑138/+45) dctA ← / ← yhjK C4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,683,5850GA39.6% 119.1 / 164.1 154intergenic (‑138/+45)dctA/yhjKC4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (46/47);  new base A (30/31);  total (76/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

AGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGC  >  NC_000913/3683436‑3683733
                                                                                                                                                     |                                                                                                                                                    
    gTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGca                                                                                                                                                    >  7:136707/1‑148 (MQ=255)
    gTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGca                                                                                                                                                    >  3:4800/1‑148 (MQ=255)
    gTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGca                                                                                                                                                    <  4:4800/148‑1 (MQ=255)
         cATAGGGTGTCCTTTAGTAAAACCACAGTTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCAGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAAc                                                                                                                                               <  8:376487/148‑1 (MQ=255)
         cATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAAc                                                                                                                                               >  6:406773/1‑147 (MQ=255)
          aTAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAAc                                                                                                                                               >  3:38934/1‑147 (MQ=255)
          aTAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAAcc                                                                                                                                              <  8:58797/148‑1 (MQ=255)
           tAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAg                                                                                                                                            <  8:219717/149‑1 (MQ=255)
              ggTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGCTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCAc                                                                                                                                         >  2:386163/1‑149 (MQ=255)
                    cTTTAGTAAAACCACATGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACttttt                                                                                                                                    <  1:304769/148‑1 (MQ=255)
                    cTTTAGTAAAACCACAGTTCTTGTAAGGATATGGGGTACCTGCGGACTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACttttt                                                                                                                                    >  4:155485/1‑148 (MQ=255)
                    cTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACttttt                                                                                                                                    >  1:41668/1‑148 (MQ=255)
                     tttAGTAAAACCACATGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTa                                                                                                                                   >  5:387957/1‑148 (MQ=255)
                     tttAGTAAAACCACAGTTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTa                                                                                                                                   >  7:180655/1‑148 (MQ=255)
                       tAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAGAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTaaa                                                                                                                                 <  1:60317/148‑1 (MQ=255)
                        aGTAAAACCACAGTTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTaaat                                                                                                                                <  7:297951/148‑2 (MQ=255)
                         gTAAAACCACAGGTCTTGAAGGTTATGGGGTACCTGCGGCCTTAAAATAAAACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGttt                                                                                                                             >  4:304944/1‑149 (MQ=255)
                           aaaaCCACAGGTCTTGTAAGGTTATGGGGAACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGtttt                                                                                                                            >  6:17532/1‑149 (MQ=255)
                            aaaCCACATGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTg                                                                                                                           <  6:89464/149‑1 (MQ=255)
                            aaaCCACATGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTg                                                                                                                           <  3:222353/149‑1 (MQ=255)
                            aaaCCACATGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTg                                                                                                                           <  1:237454/149‑1 (MQ=255)
                            aaaCCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGGTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGtttt                                                                                                                            <  5:189349/148‑1 (MQ=255)
                             aaCCACAGGTCTTGTAAGGTTATGGGGTACCTGCTGCCTTAAAATAACACCCAGCCAACATAACATAAATGAACATGGATCATAATTGCAACAAAAAGGTTAAAAAGTTTGAGCTGTCTCAAACAAACCCGCAGTATTTAAAgagttc                                                                                                                           >  6:372191/1‑143 (MQ=255)
                                 acaGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGtt                                                                                                                              <  2:61250/141‑1 (MQ=255)
                                 acaGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAAt                                                                                                                       >  5:193260/1‑148 (MQ=255)
                                  caGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAAt                                                                                                                       >  7:124373/1‑147 (MQ=255)
                                   aGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAg                                                                                                                    >  3:138248/1‑149 (MQ=255)
                                     gTCTTGAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCAAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCa                                                                                                                     >  4:252945/1‑145 (MQ=255)
                                     gTCTTGAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCAAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCa                                                                                                                     <  3:252945/145‑1 (MQ=255)
                                       tcttgaaGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTg                                                                                                                <  5:143064/144‑1 (MQ=255)
                                       cTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTg                                                                                                                >  5:285724/1‑149 (MQ=255)
                                         tGTAAGGTTATGGGGTACCTGCGGCCTTAAAATACCCCCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACCAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTggg                                                                                                              >  2:277445/1‑149 (MQ=255)
                                          gTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTgggg                                                                                                             >  2:119061/1‑149 (MQ=255)
                                           tAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGt                                                                                                            <  1:252147/149‑1 (MQ=255)
                                              ggTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGc                                                                                                         <  3:382080/149‑1 (MQ=255)
                                                 tATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTAc                                                                                                      >  1:84104/1‑149 (MQ=255)
                                                           cTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCa                                                                                            <  6:158310/149‑1 (MQ=255)
                                                              cGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAg                                                                                           <  3:269221/147‑1 (MQ=255)
                                                                gCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTaa                                                                                       <  2:121830/149‑1 (MQ=255)
                                                                 ccTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAAc                                                                                      >  7:356496/1‑149 (MQ=255)
                                                                     aaaaTAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGttt                                                                                                                             <  4:210958/106‑1 (MQ=255)
                                                                     aaaaTAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGttt                                                                                                                             >  3:210958/1‑106 (MQ=255)
                                                                     aaaaTAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAActctc                                                                                  >  2:148635/1‑149 (MQ=255)
                                                                     aaaaTAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTg                                                                                                                           >  2:416982/1‑108 (MQ=255)
                                                                      aaaTAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAActctct                                                                                 <  5:406773/149‑1 (MQ=255)
                                                                       aaTAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTATTCAGTTTGTTGTAGCTACTTTTGTTCCAGGTTACTCTCtt                                                                                <  4:92835/149‑1 (MQ=255)
                                                                           acacCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGaa                                                                            <  4:119358/149‑1 (MQ=255)
                                                                           acacCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGaa                                                                            <  6:193260/149‑1 (MQ=255)
                                                                             acCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCg                                                                              >  5:341996/1‑145 (MQ=255)
                                                                               ccAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTTCGCTGGCTCGCACAAACCAGCCCTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGAtt                                                                        >  7:161135/1‑149 (MQ=255)
                                                                               ccAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCACGCACAAACCAGCACTTTTTAATGTTTTGTATTCAGTTTGGGGTTCCTACTTTTCTTCCAGGAAACTCTCTTCGATGAtt                                                                        >  6:38177/1‑149 (MQ=255)
                                                                                cAGACAACATCACAGAAATTTACCTGGATGATAAATGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAATCCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTGCAGGTAACTCTCTTCGAAGAttt                                                                       <  6:201051/149‑1 (MQ=255)
                                                                                cAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGAttt                                                                       <  8:128412/149‑1 (MQ=255)
                                                                                  gACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCa                                                                     <  7:401355/149‑1 (MQ=255)
                                                                                   acaacaTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTATGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCaa                                                                    <  5:17532/149‑1 (MQ=255)
                                                                                   acaacaTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCaa                                                                    <  4:308749/149‑1 (MQ=255)
                                                                                   acaacaTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGATGGCTCGCACAAACCAGCACTTTTTAAAGTATTGTAATCAGTTTGGGGTAGCTAATTttctta                                                                                              >  6:235912/1‑122 (MQ=255)
                                                                                     aacaTCACAGAAATGTAACTTGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGTCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTAGAAGATTTCAATa                                                                  <  5:372191/149‑1 (MQ=255)
                                                                                       caTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTATTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAgg                                                                <  5:342833/149‑1 (MQ=255)
                                                                                        aTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAgg                                                                >  5:21964/1‑148 (MQ=255)
                                                                                        aTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGGTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAgg                                                                >  1:52795/1‑148 (MQ=255)
                                                                                         tCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAgg                                                                >  6:220772/1‑147 (MQ=255)
                                                                                         tCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGGTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGa                                                              <  2:52795/149‑1 (MQ=255)
                                                                                             aGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTgg                                                          >  2:232099/1‑149 (MQ=255)
                                                                                               aaaTGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGc                                                        <  6:285724/149‑1 (MQ=255)
                                                                                               aaaTGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGc                                                        >  8:89938/1‑149 (MQ=255)
                                                                                                aaTGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGTTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGc                                                        <  7:233135/148‑1 (MQ=255)
                                                                                                     aCCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTcc                                                                                             >  6:104153/1‑106 (MQ=255)
                                                                                                     aCCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTcc                                                                                             <  5:104153/106‑1 (MQ=255)
                                                                                                       cTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCaaa                                                 >  7:226427/1‑148 (MQ=255)
                                                                                                          gATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCaaaaa                                               <  8:356496/147‑1 (MQ=255)
                                                                                                          gATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCaaaaa                                               <  2:309115/147‑1 (MQ=255)
                                                                                                          gATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCaaaaa                                               <  3:290136/147‑1 (MQ=255)
                                                                                                           aTCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGa                                                                          >  8:169680/1‑119 (MQ=255)
                                                                                                            tCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGaa                                           <  1:148635/149‑1 (MQ=255)
                                                                                                              aTAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAg                                          <  8:136707/148‑1 (MQ=255)
                                                                                                               tAATTGAAACAAAAAGGTTATATAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGcc                                        >  4:56748/1‑149 (MQ=255)
                                                                                                               tAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGcc                                        >  5:305133/1‑149 (MQ=255)
                                                                                                                aaTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCAAGGTAAGTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGACGccc                                       >  1:379015/1‑149 (MQ=255)
                                                                                                                     aaaCAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCaaa                                                 <  8:24582/134‑1 (MQ=255)
                                                                                                                     aaaCAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTggg                                   <  6:387957/148‑1 (MQ=255)
                                                                                                                       aCAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCaa                                >  1:404660/1‑149 (MQ=255)
                                                                                                                        cAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAAt                               <  6:55037/149‑1 (MQ=255)
                                                                                                                         aaaaaGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTcc                                                                                             >  7:107661/1‑86 (MQ=255)
                                                                                                                         aaaaaGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTcc                                                                                             <  8:107661/86‑1 (MQ=255)
                                                                                                                         aaaaaGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTATCTCTCTTCGAAGATTTCAAGAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATa                              >  7:426007/1‑149 (MQ=255)
                                                                                                                         aaaaaGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGaa                                                                            >  3:235328/1‑103 (MQ=255)
                                                                                                                         aaaaaGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGaa                                                                            <  4:235328/103‑1 (MQ=255)
                                                                                                                          aaaaGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACGTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATAc                             >  2:41867/1‑149 (MQ=255)
                                                                                                                            aaGGTTAAAAAGTTTGAGCTGGCTCGCACAACCCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCa                           >  5:195849/1‑149 (MQ=255)
                                                                                                                                 tAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGc                       >  2:172158/1‑148 (MQ=255)
                                                                                                                                    aaaGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGc                   >  4:71315/1‑149 (MQ=255)
                                                                                                                                     aaGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCATCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCt                  <  1:119061/149‑1 (MQ=255)
                                                                                                                                        tttGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCtttt               <  1:372132/149‑1 (MQ=255)
                                                                                                                                                 gCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCttt                <  8:313426/139‑1 (MQ=255)
                                                                                                                                                     gCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTgg                                                                                                               >  3:1965/1‑40 (MQ=255)
                                                                                                                                                     gCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTcgc  >  7:645/1‑149 (MQ=255)
                                                                                                                                                     gCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTcgc  <  1:41867/149‑1 (MQ=255)
                                                                                                                                                     gCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTcgc  <  8:645/149‑1 (MQ=255)
                                                                                                                                                     gCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCAAGCCAGTcgc  >  4:224577/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGC  >  NC_000913/3683436‑3683733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: