Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,683,473 G→T 11.6% intergenic (‑26/+157) dctA ← / ← yhjK C4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,683,4730GT11.6% 439.5 / 37.3 151intergenic (‑26/+157)dctA/yhjKC4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (67/66);  new base T (10/8);  total (77/74)
Fisher's exact test for biased strand distribution p-value = 8.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.49e-01

Only 100 of 153 total aligned reads displayed.

CCGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAG  >  NC_000913/3683329‑3683619
                                                                                                                                                |                                                                                                                                                  
ccGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCtt                                                                                                                                                <  7:143300/149‑1 (MQ=255)
ccGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCtt                                                                                                                                                <  7:226033/149‑1 (MQ=255)
 cGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTg                                                                                                                                               <  3:232042/149‑1 (MQ=255)
 cGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTg                                                                                                                                               <  2:408965/149‑1 (MQ=255)
  gTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTc                                                                                                                                                  >  6:320642/1‑145 (MQ=255)
  gTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTg                                                                                                                                               >  6:232190/1‑148 (MQ=255)
   tCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGAACTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGt                                                                                                                                              >  4:20308/1‑148 (MQ=255)
     gCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTTCTTGTAAg                                                                                                                                           >  7:376487/1‑149 (MQ=255)
          gCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTAt                                                                                                                                      <  4:143458/149‑1 (MQ=255)
          gCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGGGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTAt                                                                                                                                      <  5:232190/149‑1 (MQ=255)
             gTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCCATGGCTATCGCTGTCAGGACCTGAAAGTCAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATggg                                                                                                                                   <  2:251628/149‑1 (MQ=255)
             gTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATggg                                                                                                                                   >  8:233135/1‑149 (MQ=255)
              tttCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATgggg                                                                                                                                  >  8:174835/1‑149 (MQ=255)
                tCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATggg                                                                                                                                   >  7:344017/1‑146 (MQ=255)
                tCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATggg                                                                                                                                   <  8:344017/146‑1 (MQ=255)
                tCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTa                                                                                                                                >  8:123256/1‑149 (MQ=255)
                   tttGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGAAGGTTATGGGGTACCTg                                                                                                                            >  7:129417/1‑149 (MQ=255)
                        tCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAATGGCTTTTAAACAGAGAGGTTTTCATTGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGc                                                                                                                        >  3:317177/1‑149 (MQ=255)
                        tCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTAATAGGGTGTCCTTTAGTAAAACCACAGTTCTTGTAAGGTTATGGGGTACCTGCgt                                                                                                                         >  3:275/1‑147 (MQ=255)
                          gCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGcc                                                                                                                       <  1:403571/148‑1 (MQ=255)
                          gCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCt                                                                                                                      >  8:297951/1‑149 (MQ=255)
                          gCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCt                                                                                                                      <  1:166739/149‑1 (MQ=255)
                            cTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCATAGGTCTTGTAAGGTTATg                                                                                                                                     >  1:119573/1‑132 (MQ=255)
                               tttCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTTCTTGTAAGGTTATGGGGTACCTGCGGCCTTaa                                                                                                                   <  3:156912/147‑1 (MQ=255)
                               tttCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTGTTACACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCTGCCTTaaaa                                                                                                                 >  6:189349/1‑149 (MQ=255)
                                  cAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGAAGGTTATGGGGTAc                                                                                                                               <  2:319558/131‑1 (MQ=255)
                                  cAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGAAGGTTATGGGGTAc                                                                                                                               >  1:319558/1‑131 (MQ=255)
                                    ggATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAaca                                                                                                            <  7:123256/149‑1 (MQ=255)
                                      aTAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACAcc                                                                                                          >  7:185669/1‑149 (MQ=255)
                                      aTAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACAcc                                                                                                          <  3:34384/149‑1 (MQ=255)
                                          aaaTGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGa                                                                                                      >  6:143064/1‑148 (MQ=255)
                                           aaTGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGac                                                                                                     <  3:234154/149‑1 (MQ=255)
                                                 cAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGGGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATc                                                                                               <  8:268704/149‑1 (MQ=255)
                                                    ggAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATcaca                                                                                            <  2:388291/149‑1 (MQ=255)
                                                        aaTACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGaaa                                                                                        <  8:361087/149‑1 (MQ=255)
                                                        aaTACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGaaa                                                                                        <  2:146874/149‑1 (MQ=255)
                                                         aTACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCATAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAAt                                                                                       <  7:320482/149‑1 (MQ=255)
                                                          tACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCATAGGTCTTGTAAGGTTATggg                                                                                                                                   >  4:333329/1‑104 (MQ=255)
                                                              aaTGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGAAGGTTATGGGGTACCTGCGGCCTTaaaa                                                                                                                 <  5:215647/117‑1 (MQ=255)
                                                                  gCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGAAGGTTAAGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGa                                                                              <  1:38927/148‑1 (MQ=255)
                                                                     aTCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCAGAGGGGGTCCTTTAGTAAAACCACAGGTCTGGTAAGGTTATGGGGTACCTGCGGCCTATAATTAACACCCAGACAACATCACAGAAATGTACCTGGATCa                                                                           >  4:269221/1‑149 (MQ=255)
                                                                          tGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAAtt                                                                      >  7:109019/1‑149 (MQ=255)
                                                                                    tGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATGGGTTCCTTCGGCCTTAAAATAAAACCCCGACAAAATAACAGAAATTTTCCTGGATCATAATTTAAACAAAAAg                                                           >  5:400717/1‑149 (MQ=255)
                                                                                     gAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAg                                                           >  8:10224/1‑149 (MQ=255)
                                                                                      aaaGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAg                                                           <  3:69793/148‑1 (MQ=255)
                                                                                      aaaGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAg                                                           <  2:157860/148‑1 (MQ=255)
                                                                                         gTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTa                                                       >  1:121830/1‑148 (MQ=255)
                                                                                           aaaGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTAACTGCGGACTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTaa                                                      >  3:119358/1‑148 (MQ=255)
                                                                                             aGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAg                                                  <  1:371065/149‑1 (MQ=255)
                                                                                                cTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGtt                                                <  8:109019/149‑1 (MQ=255)
                                                                                                 ttttAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGttt                                               >  7:142439/1‑148 (MQ=255)
                                                                                                 atttAAACAGATAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACATGGATCAGAATAGAAACAAAAAGGTTAAAAAGatt                                               >  4:290136/2‑148 (MQ=255)
                                                                                                  tttAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGttt                                               >  5:89464/1‑148 (MQ=255)
                                                                                                   ttAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGttt                                               <  3:196904/147‑1 (MQ=255)
                                                                                                   ttAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGttt                                               <  7:2552/147‑1 (MQ=255)
                                                                                                   ttAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGCAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAAt                                                                       >  3:51271/1‑123 (MQ=255)
                                                                                                   ttAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGCAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAAt                                                                       <  4:51271/123‑1 (MQ=255)
                                                                                                    tAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGa                                             <  7:56249/148‑1 (MQ=255)
                                                                                                    tAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAg                                            <  6:183566/148‑1 (MQ=255)
                                                                                                     aaaCAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTg                                              <  6:130661/146‑1 (MQ=255)
                                                                                                       aCAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTg                                         >  4:222353/1‑149 (MQ=255)
                                                                                                          gagagGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCt                                      <  3:391082/149‑1 (MQ=255)
                                                                                                           agagGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTc                                     >  7:368255/1‑149 (MQ=255)
                                                                                                           agagGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCt                                      >  3:308749/1‑148 (MQ=255)
                                                                                                           agagGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTc                                     >  4:382080/1‑149 (MQ=255)
                                                                                                           agagGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCg                                    >  7:128412/1‑149 (MQ=255)
                                                                                                             agGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGTTCATAATTGAAACAAAAAGGGTAAAAAGTTTGAGCTGGCTcac                                   >  1:351691/1‑147 (MQ=255)
                                                                                                               gTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGttt                                               <  6:333788/135‑1 (MQ=255)
                                                                                                               gTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGttt                                               >  5:333788/1‑135 (MQ=255)
                                                                                                               gTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGca                                  <  4:4800/148‑1 (MQ=255)
                                                                                                                    cATAGGGTGTCCTTTAGTAAAACCACAGTTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCAGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAAc                             <  8:376487/148‑1 (MQ=255)
                                                                                                                    cATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAAc                             >  6:406773/1‑147 (MQ=255)
                                                                                                                     aTAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAAc                             >  3:38934/1‑147 (MQ=255)
                                                                                                                     aTAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAAcc                            <  8:58797/148‑1 (MQ=255)
                                                                                                                      tAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAg                          <  8:219717/149‑1 (MQ=255)
                                                                                                                         ggTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGCTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCAc                       >  2:386163/1‑149 (MQ=255)
                                                                                                                               cTTTAGTAAAACCACAGTTCTTGTAAGGATATGGGGTACCTGCGGACTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACttttt                  >  4:155485/1‑148 (MQ=255)
                                                                                                                                tttAGTAAAACCACATGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTa                 >  5:387957/1‑148 (MQ=255)
                                                                                                                                tttAGTAAAACCACATGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTa                 >  8:401355/1‑148 (MQ=255)
                                                                                                                                tttAGTAAAACCACAGTTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTa                 >  7:180655/1‑148 (MQ=255)
                                                                                                                                  tAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAGAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTaaa               <  1:60317/148‑1 (MQ=255)
                                                                                                                                    gTAAAACCACAGGTCTTGAAGGTTATGGGGTACCTGCGGCCTTAAAATAAAACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGttt           >  4:304944/1‑149 (MQ=255)
                                                                                                                                     tAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGCGCTGGCTCAGACAAACCAGCACTTTTTaatgggt           >  2:237454/1‑144 (MQ=255)
                                                                                                                                     tAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACACAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGttt           >  6:342833/1‑149 (MQ=255)
                                                                                                                                       aaaCCACATGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTg         <  6:89464/149‑1 (MQ=255)
                                                                                                                                       aaaCCACATGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTg         <  1:237454/149‑1 (MQ=255)
                                                                                                                                       aaaCCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTg         >  3:219262/1‑149 (MQ=255)
                                                                                                                                       aaaCCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGGTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGtttt          <  5:189349/148‑1 (MQ=255)
                                                                                                                                       aaaCCACAGGTCTTGTAAGGCTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTg         <  1:386163/149‑1 (MQ=255)
                                                                                                                                        aaCCACAGGTCTTGTAAGGTTATGGGGTACCTGCTGCCTTAAAATAACACCCAGCCAACATAACATAAATGAACATGGATCATAATTGCAACAAAAAGGTTAAAAAGTTTGAGCTGTCTCAAACAAACCCGCAGTATTTAAAgagttc         >  6:372191/1‑143 (MQ=255)
                                                                                                                                          ccACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTaa      <  2:41668/149‑1 (MQ=255)
                                                                                                                                            acaGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGtt            <  2:61250/141‑1 (MQ=255)
                                                                                                                                            acaGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGtt            >  1:61250/1‑141 (MQ=255)
                                                                                                                                            acaGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAAt     >  5:193260/1‑148 (MQ=255)
                                                                                                                                            acaGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACAACCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAAt     >  7:84300/1‑148 (MQ=255)
                                                                                                                                            acaGGTCTTGAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCa   <  8:142439/149‑1 (MQ=255)
                                                                                                                                             caGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTAAAATAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCa   <  5:112902/149‑1 (MQ=255)
                                                                                                                                              aGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAg  >  3:138248/1‑149 (MQ=255)
                                                                                                                                                gTCTTGAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCAAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCa   <  3:252945/145‑1 (MQ=255)
                                                                                                                                                gTCTTGAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCAAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCa   >  4:252945/1‑145 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CCGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAG  >  NC_000913/3683329‑3683619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: