Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,951,471 G→A 7.0% R331C (CGC→TGC)  recD ← exonuclease V (RecBCD complex), alpha chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,951,4710GA 7.0% 266.2 / 5.8 85R331C (CGC→TGC) recDexonuclease V (RecBCD complex), alpha chain
Reads supporting (aligned to +/- strand):  ref base G (41/38);  new base A (2/4);  total (43/42)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

TATCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAG  >  NC_000913/2951327‑2951618
                                                                                                                                                |                                                                                                                                                   
tATCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCt                                                                                                                                                  <  8:217116/148‑1 (MQ=255)
  tCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTa                                                                                                                                                <  2:67077/148‑1 (MQ=255)
  tCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAg                                                                                                                                               <  5:231485/149‑1 (MQ=255)
  tCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAg                                                                                                                                               >  5:361872/1‑149 (MQ=255)
  tCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAg                                                                                                                                               >  5:368216/1‑149 (MQ=255)
  tCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAg                                                                                                                                               >  6:233007/1‑149 (MQ=255)
    accacGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCt                                                                                                                                             <  8:135119/149‑1 (MQ=255)
    accacGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAAGCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGc                                                                                                                                              <  7:291541/148‑1 (MQ=255)
      cacGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGc                                                                                                                                           <  5:374000/149‑1 (MQ=255)
        cGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTTCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCt                                                                                                                                         <  7:388675/149‑1 (MQ=255)
        cGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCt                                                                                                                                         <  7:242910/149‑1 (MQ=255)
         ggTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTg                                                                                                                                        <  7:111411/149‑1 (MQ=255)
          gTTGATCGCCGCATCTAACTGACCTATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCACGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTGCTGCCGGAACGTGAGTCCGCGTCAGGCAGCTAAGCCGCCTga                                                                                                                                       <  6:409046/149‑2 (MQ=255)
          gTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTgg                                                                                                                                       <  6:373397/149‑1 (MQ=255)
             gATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCAc                                                                                                                                    >  5:162178/1‑149 (MQ=255)
              aTCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACg                                                                                                                                   <  6:130971/149‑1 (MQ=255)
              aTCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGCCAGGCAGCTAAGCCGCCTgacggg                                                                                                                                   <  6:201626/149‑6 (MQ=255)
              aTCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCAGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACg                                                                                                                                   >  3:242736/1‑149 (MQ=255)
               tCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGc                                                                                                                                  >  8:902/1‑149 (MQ=255)
               tCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGc                                                                                                                                  >  3:181953/1‑149 (MQ=255)
                 gccgcAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTc                                                                                                                                <  6:82480/149‑1 (MQ=255)
                 gccgcAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCAGCTAAGCCGCCTgacgg                                                                                                                                    >  2:192155/1‑141 (MQ=255)
                 gccgcAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCAGCTAAGCCGCCTgacgg                                                                                                                                    <  1:192155/145‑5 (MQ=255)
                   cgcAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCGTGGCACGCTcgg                                                                                                                              >  1:196566/1‑149 (MQ=255)
                   cgcAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTcgg                                                                                                                              >  1:407470/1‑149 (MQ=255)
                      aGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTcggcgg                                                                                                                           >  7:262683/1‑149 (MQ=255)
                               aCCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGCGGCCCCGTCAGGCGGCTTAGCTGCCTGGCCCGCTCGGCGGTAACGCCcg                                                                                                                  >  7:154963/1‑149 (MQ=255)
                               aCCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCcg                                                                                                                  >  3:272623/1‑149 (MQ=255)
                                 cAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGCCAGGCAGCTAAGCCGCCTgacgg                                                                                                                                    <  5:359794/129‑5 (MQ=255)
                                 cAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGCCAGGCAGCTAAGCCGCCTgacgg                                                                                                                                    >  6:359794/1‑125 (MQ=255)
                                     gCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTgg                                                                                                            >  1:15637/1‑149 (MQ=255)
                                      ccAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTgg                                                                                                            <  8:92717/148‑1 (MQ=255)
                                      ccAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGc                                                                                                           <  4:181953/149‑1 (MQ=255)
                                       cAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGc                                                                                                           >  4:176777/1‑148 (MQ=255)
                                       cAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCa                                                                                                          >  4:72671/1‑149 (MQ=255)
                                        aGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCa                                                                                                          >  1:265491/1‑148 (MQ=255)
                                          aaTCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATaa                                                                                                       <  8:135505/149‑1 (MQ=255)
                                               cTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCa                                                                                                  <  6:413547/149‑1 (MQ=255)
                                                  ccGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGa                                                                                                >  6:83502/1‑148 (MQ=255)
                                                   cGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGata                                                                                              >  8:331215/1‑149 (MQ=255)
                                                     aaaCGATAGCTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTGCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCg                                                                                           <  5:137067/149‑1 (MQ=255)
                                                         gATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGccc                                                                                        <  5:233007/149‑1 (MQ=255)
                                                         gATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGccc                                                                                        <  2:256101/149‑1 (MQ=255)
                                                          aTAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGcc                                                                                         >  6:141237/1‑147 (MQ=255)
                                                           tAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAgcg                                                                                                    <  2:284520/135‑1 (MQ=255)
                                                           tAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAgcg                                                                                                    >  1:284520/1‑135 (MQ=255)
                                                               ttttttGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGca                                                                                    >  4:385831/1‑147 (MQ=255)
                                                                    tGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGccc                                                                              <  8:116083/148‑1 (MQ=255)
                                                                      cagcagGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGGCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAg                                                                           <  1:10480/149‑1 (MQ=255)
                                                                       agcagGCAGAGACTGGCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGc                                                                          <  8:120022/149‑1 (MQ=255)
                                                                              agagACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCTCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAAcc                                                                   >  1:60450/1‑149 (MQ=255)
                                                                               gagaCTGGCGCGCAACGATGCCCCTTCTGTGCCGGTTCCTGCCGGTACGTGAGTCCCCGTCCGGCGGCGTAGCTGCCTTGAACGCTCGGCGGTAAAGCCCGCGTTTGCATAAGCTCAGATATCGCACAGCTCTGCCCCAGCCGCAACCg                                                                  <  4:272468/149‑1 (MQ=255)
                                                                                 gaCTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAg                                                                <  5:141237/149‑1 (MQ=255)
                                                                                    tGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGc                                                              >  7:57281/1‑148 (MQ=255)
                                                                                      tCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAg                                                           >  1:308290/1‑149 (MQ=255)
                                                                                       cgcgCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGt                                                          >  3:181672/1‑149 (MQ=255)
                                                                                          gcAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGa                                                       <  3:362334/149‑1 (MQ=255)
                                                                                           cAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGAt                                                      >  7:283853/1‑149 (MQ=255)
                                                                                             aaGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGTCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCa                                                    <  2:265491/149‑1 (MQ=255)
                                                                                                aTGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGa                                                 <  2:196566/149‑1 (MQ=255)
                                                                                                 tGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGAt                                                <  8:262683/149‑1 (MQ=255)
                                                                                                 tGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGAt                                                >  1:224314/1‑149 (MQ=255)
                                                                                                   ccGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCg                                              >  4:45307/1‑149 (MQ=255)
                                                                                                   ccGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCg                                              <  5:83502/149‑1 (MQ=255)
                                                                                                   ccGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCg                                              >  8:28286/1‑149 (MQ=255)
                                                                                                     gctgctGTGCCTGGTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGAGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGcc                                            <  8:154963/149‑1 (MQ=255)
                                                                                                         cTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCga                                          <  3:179572/147‑1 (MQ=255)
                                                                                                            tgCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCgaga                                        >  1:122317/1‑146 (MQ=255)
                                                                                                            tgCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCgaga                                        >  5:327326/1‑146 (MQ=255)
                                                                                                            tgCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGaaa                                      <  4:272623/148‑1 (MQ=255)
                                                                                                              ccGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCgaga                                        >  6:137708/1‑144 (MQ=255)
                                                                                                              ccGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGAt                                   <  2:407470/149‑1 (MQ=255)
                                                                                                                 gTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCAc                                >  4:273451/1‑149 (MQ=255)
                                                                                                                    ccTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATACGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTc                              <  8:347209/148‑1 (MQ=255)
                                                                                                                     cTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTcgc                            >  4:217055/1‑149 (MQ=255)
                                                                                                                      tGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTcgcg                           >  6:411137/1‑149 (MQ=255)
                                                                                                                         cGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCAt                        >  2:388309/1‑149 (MQ=255)
                                                                                                                            aaCGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGAt                     <  3:176777/149‑1 (MQ=255)
                                                                                                                              cGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCg                   >  3:182327/1‑149 (MQ=255)
                                                                                                                               gTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCgg                  <  4:181672/149‑1 (MQ=255)
                                                                                                                                    tCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAg             >  7:302078/1‑149 (MQ=255)
                                                                                                                                      cccGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCggg                 >  8:140805/1‑143 (MQ=255)
                                                                                                                                      cccGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCggg                 <  7:140805/143‑1 (MQ=255)
                                                                                                                                      cccGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGc           >  8:38340/1‑149 (MQ=255)
                                                                                                                                       ccGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCg          >  3:61100/1‑149 (MQ=255)
                                                                                                                                             ggcggcTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGAt     >  3:182080/1‑148 (MQ=255)
                                                                                                                                              gcggcTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCTATCa   <  5:334135/149‑1 (MQ=255)
                                                                                                                                               cggcTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAg  >  2:293330/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TATCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAG  >  NC_000913/2951327‑2951618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: