BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,683,479	T→C	6.0%	intergenic (‑32/+151)	dctA ← / ← yhjK	C4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,683,479	0	T	C	6.0%	411.6 / 11.5	133	intergenic (‑32/+151)	dctA/yhjK	C4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand): ref base T (69/56); new base C (4/4); total (73/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

CGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGG  >  NC_000913/3683333‑3683625

cGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGt                                                                                                                                                    >  4:85049/1‑147 (MQ=255)

cGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGt                                                                                                                                                    >  8:171145/1‑147 (MQ=255)

 gCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGCTGTTTTCATAGGGTGTCCTTTAGTAAAACCACATATCTTGTa                                                                                                                                                   <  1:122137/147‑1 (MQ=255)

 gCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTaa                                                                                                                                                  <  5:297115/148‑1 (MQ=255)

 gCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTa                                                                                                                                                   <  7:171469/147‑1 (MQ=255)

     agccgcttCCTTTGCCCGCCTATTTCACGACACAAATCCCCAAGGAGAATACCAATGGCTATCGCGGTCAGGACCTGAAAGTAAACGCTCTTAAACAGAGAGGGTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGtt                                                                                                                                              <  4:355910/142‑1 (MQ=255)

     aGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTTAAACCACAGGTCTTGCAAGGt                                                                                                                                               >  6:330707/1‑147 (MQ=255)

     aGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGt                                                                                                                                               >  2:276414/1‑147 (MQ=255)

       gggTTTCTTTTGGTCGCGTTTTTCTGGATTGTATTGGGCTAGGAGTATTCGTTTGGCTATCGCTGTCAGGACCTGAAAGTTAAGGCTTTTAAACAGTGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTa                                                                                                                                             <  4:432167/146‑1 (MQ=255)

       cGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTAt                                                                                                                                            <  8:372750/148‑1 (MQ=255)

         gTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATgg                                                                                                                                          <  1:350993/148‑1 (MQ=255)

             cATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTaa                                                                                                                                                  <  4:130952/136‑1 (MQ=255)

               tttGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTAcc                                                                                                                                    >  2:466264/1‑148 (MQ=255)

                    tCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCgg                                                                                                                               >  7:254909/1‑148 (MQ=255)

                         tATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTAcc                                                                                                                                    <  4:23379/138‑1 (MQ=255)

                         tATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTAcc                                                                                                                                    >  3:23379/1‑138 (MQ=255)

                          aTTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTa                                                                                                                          >  5:166665/1‑147 (MQ=255)

                          aTTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTa                                                                                                                          >  5:342501/1‑147 (MQ=255)

                          aTTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGtt                                                                                                                                              >  5:437062/1‑127 (MQ=255)

                          aTTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGtt                                                                                                                                              <  6:437062/127‑1 (MQ=255)

                           tttCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTTCTTGTAAGGTTATGGGGTACCTGCGGCCTTaa                                                                                                                         <  8:269161/147‑1 (MQ=255)

                           tttCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTaaa                                                                                                                        <  2:236080/148‑1 (MQ=255)

                            ttCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTaaaa                                                                                                                       >  7:108099/1‑148 (MQ=255)

                              cAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAAt                                                                                                                      <  6:19656/147‑1 (MQ=255)

                               aGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATa                                                                                                                     <  6:166665/147‑1 (MQ=255)

                               aGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATa                                                                                                                     <  4:244295/147‑1 (MQ=255)

                                tgATAGAAATGGAGAAGGAGAATACCAATGGCTTTCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTACTTTAGTAAAACCACAAGTATTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAac                                                                                                                   <  8:254909/147‑1 (MQ=255)

                                 taTAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAaca                                                                                                                  <  5:32884/147‑1 (MQ=255)

                                    aGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCta                                                                                                                                  <  7:144755/129‑2 (MQ=255)

                                     gAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGCAAGGTTATGGGGTACCTGCGGCCTTAAAATAACAccc                                                                                                               <  2:12181/147‑1 (MQ=255)

                                         tGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATa                                                                                                                     <  2:108117/137‑1 (MQ=255)

                                         tGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATa                                                                                                                     >  1:108117/1‑137 (MQ=255)

                                             cAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACAt                                                                                                      >  3:272408/1‑148 (MQ=255)

                                                 gagaATACAAATGGATATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTAAGTAAAACCAAAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATcaca                                                                                                  >  1:326557/1‑148 (MQ=255)

                                                   accaatggctatcgctgtcaggacctgaaagtaaaggcttttacacagagaggtgttcatagggtgtcctttagtaaatccacagACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATACCACCCAGACAACATCACAg                                                                                                 >  5:203925‑M2/86‑147 (MQ=255)

                                                    aaTACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGaa                                                                                               <  6:261682/148‑1 (MQ=255)

                                                     aTACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGCAAGGTTATGGTGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGaa                                                                                               <  6:78386/147‑1 (MQ=255)

                                                      aatggctatcgctgtcaggacctgaaagtaaaggcttttaaacagagaggttttcatagggtgtcctttagtaaaaccacagACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCa                                                                                                              <  6:316404‑M2/49‑1 (MQ=255)

                                                       aCCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATg                                                                                            >  7:222940/1‑148 (MQ=255)

                                                       aCCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATg                                                                                            >  6:444010/1‑148 (MQ=255)

                                                         cAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTa                                                                                          <  2:238317/148‑1 (MQ=255)

                                                          gctatcgctgtcaggacctgaaagtaaaggcttttaaacagagaggttttcatagggtgtcctttagtaaaaccacagACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTa                                                                                          <  7:483062‑M2/69‑1 (MQ=255)

                                                            tGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTAATAGGGTGTCCTTTATTAAAACCACAGGTCTTGTAAGGTTATGGGGTACATACGGCCTTAAAATAAAACACAGACAACATCACACAAATGTACCt                                                                                       >  3:129515/1‑148 (MQ=255)

                                                             ggCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTg                                                                                      >  4:354844/1‑148 (MQ=255)

                                                             ggCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGGCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTg                                                                                      >  2:381274/1‑148 (MQ=255)

                                                                 aTCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTAt                                                                                                                                            <  1:218149/90‑1 (MQ=255)

                                                                  tCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGacaac                                                                                                        >  2:210163/1‑125 (MQ=255)

                                                                  tCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGacaac                                                                                                        <  1:210163/125‑1 (MQ=255)

                                                                          aGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGa                                                                          <  1:276414/147‑1 (MQ=255)

                                                                           ggACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGaaa                                                                        >  7:200183/1‑148 (MQ=255)

                                                                              ccTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGCAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACaa                                                                     <  3:369155/148‑1 (MQ=255)

                                                                                tGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACaaa                                                                    >  1:128273/1‑147 (MQ=255)

                                                                                tGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACaaa                                                                    >  4:302265/1‑147 (MQ=255)

                                                                                 gAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACaaaa                                                                   >  5:195025/1‑147 (MQ=255)

                                                                                  aaaGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAg                                                                 >  4:183585/1‑148 (MQ=255)

                                                                                  aaaGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGAGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACaaaaa                                                                  <  3:281099/147‑1 (MQ=255)

                                                                                       aaaGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTa                                                                                          >  8:100873/1‑118 (MQ=255)

                                                                                       aaaGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTa                                                             >  3:404678/1‑147 (MQ=255)

                                                                                       aaaGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGCAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTa                                                             >  6:282164/1‑147 (MQ=255)

                                                                                       aaaGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACAACAGGTCTTGTAAGGTTTTGGGGTACCTACGGCCTTAAAATAAAACCCAGACAAAATCACAGAAATGTACCTGGAAAATAATTGAAACAAAAAGGATa                                                             >  3:411059/1‑147 (MQ=255)

                                                                                        aaGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTaa                                                            >  8:469165/1‑147 (MQ=255)

                                                                                            cTTTTAAACTGAGAGGTTTTCATCTGGTGTCATTTAGTAAATCCACTGTTATTGTAAGGTTTTGGGGTAACTGAGGAATTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAATAAGTGTAAAAAGt                                                       <  5:308352/148‑1 (MQ=255)

                                                                                              tttAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGCAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGttt                                                     >  8:36525/1‑148 (MQ=255)

                                                                                               ttAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAAAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGttt                                                     >  7:117483/1‑147 (MQ=255)

                                                                                               ttAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAAGGTACCTGGATCATAATTGAAACAAAAAGGTAAAAAAGTTTg                                                    >  6:66625/1‑148 (MQ=255)

                                                                                                tAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTg                                                    <  7:171145/147‑1 (MQ=255)

                                                                                                tAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTg                                                    <  7:469165/147‑1 (MQ=255)

                                                                                                  aaCAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAg                                                  <  8:222940/147‑1 (MQ=255)

                                                                                                    cAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCGCAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTg                                               >  8:156996/1‑148 (MQ=255)

                                                                                                      gagagGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGc                                             >  1:255630/1‑148 (MQ=255)

                                                                                                       agagGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATcac                                                                                                   >  6:106851/1‑93 (MQ=255)

                                                                                                          ggTTTTCATAGGGTGTCCTTTAGTAAAACCAAAGGTCTTGTAAGGTTATCGGGTACCTGCGGCCTTAAAATAACACCCAGACATCATCACAGAAATGAACATGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTTAGCTGGCTCg                                          >  4:259509/1‑147 (MQ=255)

                                                                                                             tttCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGa                                                   >  8:467164/1‑135 (MQ=255)

                                                                                                                cATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACaaa                                    <  5:444010/147‑1 (MQ=255)

                                                                                                                        gtCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACtt                           >  7:343677/1‑148 (MQ=255)

                                                                                                                         tCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACtt                           <  6:342501/147‑1 (MQ=255)

                                                                                                                          ccTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACtttt                         >  5:456443/1‑148 (MQ=255)

                                                                                                                            tttAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACttttt                        >  2:101108/1‑147 (MQ=255)

                                                                                                                            tttAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACttttt                        >  7:470022/1‑147 (MQ=255)

                                                                                                                             ttAGTAAAACCACAGGTCCTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTa                       >  4:281374/1‑147 (MQ=255)

                                                                                                                              aaaaccacagACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTaaa                     >  2:79089‑M2/11‑148 (MQ=255)

                                                                                                                                 tAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGtt                  >  6:10259/1‑148 (MQ=255)

                                                                                                                                    aaCCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTg               >  1:28324/1‑148 (MQ=255)

                                                                                                                                    aaCCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTg               >  1:20974/1‑148 (MQ=255)

                                                                                                                                       cacaGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTaa            >  4:66763/1‑148 (MQ=255)

                                                                                                                                           ggTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAg        >  1:393716/1‑148 (MQ=255)

                                                                                                                                            gTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGt       <  7:399203/148‑1 (MQ=255)

                                                                                                                                            gTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGt       <  1:425492/148‑1 (MQ=255)

                                                                                                                                             tCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGtt      <  3:333306/148‑1 (MQ=255)

                                                                                                                                             tCTTGCAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGtt      <  5:282164/148‑1 (MQ=255)

                                                                                                                                              cTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACAACCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGATTGAGCTGGCTCGAACAAACCAGCACTTTTTAAAGTTTTTTAATCAGttt     >  4:294540/1‑148 (MQ=255)

                                                                                                                                                tGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTgg   >  2:205721/1‑148 (MQ=255)

                                                                                                                                                tGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTg    >  1:407252/1‑147 (MQ=255)

                                                                                                                                                tGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTgg   >  8:1163/1‑148 (MQ=255)

                                                                                                                                                 gTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGtt      <  8:118353/144‑1 (MQ=255)

                                                                                                                                                 gTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTgg   <  8:343677/147‑1 (MQ=255)

                                                                                                                                                 gTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCACACAAACCAGCACTTTTTAAAGTTTTGTAATCAGttt     <  2:407252/145‑1 (MQ=255)

                                                                                                                                                 gTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTgg   <  7:1163/147‑1 (MQ=255)

                                                                                                                                                 gTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTg    <  2:255454/146‑1 (MQ=255)

                                                                                                                                                  tAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTggg  <  3:200913/147‑1 (MQ=255)

CGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGG  >  NC_000913/3683333‑3683625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`