Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,683,473 G→A 5.0% intergenic (‑26/+157) dctA ← / ← yhjK C4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,683,4730GA 5.0% 384.6 / 6.2 121intergenic (‑26/+157)dctA/yhjKC4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (66/44);  new base A (2/4);  total (70/51)
Fisher's exact test for biased strand distribution p-value = 2.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.05e-01

Only 100 of 125 total aligned reads displayed.

AGCCGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGT  >  NC_000913/3683327‑3683620
                                                                                                                                                  |                                                                                                                                                   
aGCCGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGt                                                                                                                                                    <  6:421055/148‑1 (MQ=255)
      cGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGt                                                                                                                                               >  8:399203/1‑147 (MQ=255)
      cGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGt                                                                                                                                               >  4:85049/1‑147 (MQ=255)
      cGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGt                                                                                                                                               >  8:171145/1‑147 (MQ=255)
       gCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGCTGTTTTCATAGGGTGTCCTTTAGTAAAACCACATATCTTGTa                                                                                                                                              <  1:122137/147‑1 (MQ=255)
       gCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTa                                                                                                                                              <  7:171469/147‑1 (MQ=255)
           aGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTTAAACCACAGGTCTTGCAAGGt                                                                                                                                          >  6:330707/1‑147 (MQ=255)
           aGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGt                                                                                                                                          >  2:276414/1‑147 (MQ=255)
            gCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTa                                                                                                                                        <  5:47474/148‑1 (MQ=255)
             gggTTTCTTTTGGTCGCGTTTTTCTGGATTGTATTGGGCTAGGAGTATTCGTTTGGCTATCGCTGTCAGGACCTGAAAGTTAAGGCTTTTAAACAGTGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTa                                                                                                                                        <  4:432167/146‑1 (MQ=255)
             cGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTAt                                                                                                                                       <  8:372750/148‑1 (MQ=255)
               gTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATgg                                                                                                                                     <  1:350993/148‑1 (MQ=255)
                   cATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTaa                                                                                                                                             <  4:130952/136‑1 (MQ=255)
                   cATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTaa                                                                                                                                             >  3:130952/1‑136 (MQ=255)
                     tttGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTAcc                                                                                                                               >  2:466264/1‑148 (MQ=255)
                       tGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCt                                                                                                                              <  3:85049/147‑1 (MQ=255)
                          tCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCgg                                                                                                                          >  7:254909/1‑148 (MQ=255)
                               tATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTAcc                                                                                                                               <  4:23379/138‑1 (MQ=255)
                                aTTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTa                                                                                                                     >  5:166665/1‑147 (MQ=255)
                                aTTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTa                                                                                                                     >  5:342501/1‑147 (MQ=255)
                                aTTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGCAAGGTTATGGGGTACCTGCGGCCTTa                                                                                                                     >  4:369155/1‑147 (MQ=255)
                                aTTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGtt                                                                                                                                         <  6:437062/127‑1 (MQ=255)
                                aTTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGtt                                                                                                                                         >  5:437062/1‑127 (MQ=255)
                                 tttCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTTCTTGTAAGGTTATGGGGTACCTGCGGCCTTaa                                                                                                                    <  8:269161/147‑1 (MQ=255)
                                  ttCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTaaaa                                                                                                                  >  7:108099/1‑148 (MQ=255)
                                    cAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAAt                                                                                                                 <  6:19656/147‑1 (MQ=255)
                                     aGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATa                                                                                                                <  6:166665/147‑1 (MQ=255)
                                     aGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATa                                                                                                                <  4:244295/147‑1 (MQ=255)
                                     aGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATa                                                                                                                <  1:287181/147‑1 (MQ=255)
                                      tgATAGAAATGGAGAAGGAGAATACCAATGGCTTTCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTACTTTAGTAAAACCACAAGTATTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAac                                                                                                              <  8:254909/147‑1 (MQ=255)
                                       taTAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAaca                                                                                                             <  5:32884/147‑1 (MQ=255)
                                          aGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCta                                                                                                                             >  8:144755/1‑128 (MQ=255)
                                          aGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCta                                                                                                                             <  7:144755/129‑2 (MQ=255)
                                               tGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATa                                                                                                                >  1:108117/1‑137 (MQ=255)
                                               tGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATa                                                                                                                <  2:108117/137‑1 (MQ=255)
                                                   cAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACAt                                                                                                 >  3:272408/1‑148 (MQ=255)
                                                    aaGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACAt                                                                                                 >  7:173918/1‑147 (MQ=255)
                                                     aGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAAACACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATca                                                                                               >  5:2687/1‑148 (MQ=255)
                                                       gagaATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATca                                                                                               >  1:255454/1‑146 (MQ=255)
                                                       gagaATACAAATGGATATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTAAGTAAAACCAAAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATcaca                                                                                             >  1:326557/1‑148 (MQ=255)
                                                         accaatggctatcgctgtcaggacctgaaagtaaaggcttttacacagagaggtgttcatagggtgtcctttagtaaatccacagACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATACCACCCAGACAACATCACAg                                                                                            >  5:203925‑M2/86‑147 (MQ=255)
                                                           aTACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGCAAGGTTATGGTGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGaa                                                                                          <  6:78386/147‑1 (MQ=255)
                                                            aatggctatcgctgtcaggacctgaaagtaaaggcttttaaacagagaggttttcatagggtgtcctttagtaaaaccacagACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCa                                                                                                         <  6:316404‑M2/49‑1 (MQ=255)
                                                            aatggctatcgctgtcaggacctgaaagtaaaggcttttaaacagagaggttttcatagggtgtcctttagtaaaaccacagACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCa                                                                                                         >  5:316404‑M2/83‑131 (MQ=255)
                                                             aCCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATg                                                                                       >  7:222940/1‑148 (MQ=255)
                                                             aCCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATg                                                                                       >  6:444010/1‑148 (MQ=255)
                                                               cAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTa                                                                                     <  2:238317/148‑1 (MQ=255)
                                                                gctatcgctgtcaggacctgaaagtaaaggcttttaaacagagaggttttcatagggtgtcctttagtaaaaccacagACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTa                                                                                     <  7:483062‑M2/69‑1 (MQ=255)
                                                                aaTGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAAACACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTAc                                                                                    >  2:185255/1‑148 (MQ=255)
                                                                  tGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCt                                                                                  >  3:242086/1‑148 (MQ=255)
                                                                  tGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTAATAGGGTGTCCTTTATTAAAACCACAGGTCTTGTAAGGTTATGGGGTACATACGGCCTTAAAATAAAACACAGACAACATCACACAAATGTACCt                                                                                  >  3:129515/1‑148 (MQ=255)
                                                                   ggCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTg                                                                                 >  4:354844/1‑148 (MQ=255)
                                                                   ggCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGGCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTg                                                                                 >  2:381274/1‑148 (MQ=255)
                                                                       aTCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTAt                                                                                                                                       <  1:218149/90‑1 (MQ=255)
                                                                       aTCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTAt                                                                                                                                       >  2:218149/1‑90 (MQ=255)
                                                                        tCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGacaac                                                                                                   <  1:210163/125‑1 (MQ=255)
                                                                        tCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGacaac                                                                                                   >  2:210163/1‑125 (MQ=255)
                                                                         cGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTg                                                                                                                                                <  2:125525/79‑1 (MQ=255)
                                                                         cGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTg                                                                                                                                                >  1:125525/1‑79 (MQ=255)
                                                                           cTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATaa                                                                         <  2:260293/148‑1 (MQ=255)
                                                                              tCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAAACACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTg                                                                      <  6:2687/148‑1 (MQ=255)
                                                                                      tGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACaaa                                                               >  1:128273/1‑147 (MQ=255)
                                                                                      tGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACaaa                                                               >  4:302265/1‑147 (MQ=255)
                                                                                       gAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACaaaa                                                              >  5:195025/1‑147 (MQ=255)
                                                                                        aaaGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGAGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACaaaaa                                                             <  3:281099/147‑1 (MQ=255)
                                                                                        aaaGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACaaa                                                               <  6:195025/145‑1 (MQ=255)
                                                                                           gTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGt                                                          >  4:333306/1‑147 (MQ=255)
                                                                                             aaaGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTa                                                                                     >  8:100873/1‑118 (MQ=255)
                                                                                             aaaGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTa                                                                                     <  7:100873/118‑1 (MQ=255)
                                                                                             aaaGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTa                                                        >  3:404678/1‑147 (MQ=255)
                                                                                             aaaGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGCAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTa                                                        >  6:282164/1‑147 (MQ=255)
                                                                                             aaaGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACAACAGGTCTTGTAAGGTTTTGGGGTACCTACGGCCTTAAAATAAAACCCAGACAAAATCACAGAAATGTACCTGGAAAATAATTGAAACAAAAAGGATa                                                        >  3:411059/1‑147 (MQ=255)
                                                                                              aaGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTaa                                                       >  8:469165/1‑147 (MQ=255)
                                                                                                  cTTTTAAACTGAGAGGTTTTCATCTGGTGTCATTTAGTAAATCCACTGTTATTGTAAGGTTTTGGGGTAACTGAGGAATTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAATAAGTGTAAAAAGt                                                  <  5:308352/148‑1 (MQ=255)
                                                                                                     ttAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAAGGTACCTGGATCATAATTGAAACAAAAAGGTAAAAAAGTTTg                                               >  6:66625/1‑148 (MQ=255)
                                                                                                      tAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTg                                               <  7:469165/147‑1 (MQ=255)
                                                                                                      tAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTg                                               <  7:171145/147‑1 (MQ=255)
                                                                                                        aaCAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAg                                             <  8:222940/147‑1 (MQ=255)
                                                                                                          cAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCGCAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTg                                          >  8:156996/1‑148 (MQ=255)
                                                                                                            gagagGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGTTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGc                                        >  1:255630/1‑148 (MQ=255)
                                                                                                             agagGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATcac                                                                                              >  6:106851/1‑93 (MQ=255)
                                                                                                                ggTTTTCATAGGGTGTCCTTTAGTAAAACCAAAGGTCTTGTAAGGTTATCGGGTACCTGCGGCCTTAAAATAACACCCAGACATCATCACAGAAATGAACATGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTTAGCTGGCTCg                                     >  4:259509/1‑147 (MQ=255)
                                                                                                                   tttCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGa                                              >  8:467164/1‑135 (MQ=255)
                                                                                                                   tttCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGa                                              <  7:467164/135‑1 (MQ=255)
                                                                                                                      cATAGGGTGTCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACaaa                               <  5:444010/147‑1 (MQ=255)
                                                                                                                              gtCCTTTAGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACtt                      >  7:343677/1‑148 (MQ=255)
                                                                                                                               tCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACtt                      <  6:342501/147‑1 (MQ=255)
                                                                                                                                ccTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACtttt                    >  4:200913/1‑148 (MQ=255)
                                                                                                                                ccTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACtttt                    >  5:456443/1‑148 (MQ=255)
                                                                                                                                  tttAGTAAAACCACATGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACttttt                   >  1:60667/1‑147 (MQ=255)
                                                                                                                                  tttAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACttttt                   >  7:343009/1‑147 (MQ=255)
                                                                                                                                  tttAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACttttt                   >  7:470022/1‑147 (MQ=255)
                                                                                                                                  tttAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACttttt                   >  2:101108/1‑147 (MQ=255)
                                                                                                                                   ttAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTGCCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTa                  >  8:286977/1‑147 (MQ=255)
                                                                                                                                   ttAGTAAAACCACAGGTCCTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTa                  >  4:281374/1‑147 (MQ=255)
                                                                                                                                    tAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTACGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTa                  <  3:354844/146‑1 (MQ=255)
                                                                                                                                    aaaaccacagACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTaaa                >  2:79089‑M2/11‑148 (MQ=255)
                                                                                                                                          aaCCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTg          >  1:28324/1‑148 (MQ=255)
                                                                                                                                             cacaGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTaa       >  4:66763/1‑148 (MQ=255)
                                                                                                                                                  gTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGt  <  1:425492/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AGCCGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGT  >  NC_000913/3683327‑3683620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: