Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,424,661 C→A 5.2% noncoding (2123/2904 nt) rrlD ← 23S ribosomal RNA of rrnD operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,424,6610CA 5.2% 361.7 / 3.0 140noncoding (2123/2904 nt)rrlD23S ribosomal RNA of rrnD operon
Reads supporting (aligned to +/- strand):  ref base C (69/62);  new base A (3/6);  total (72/68)
Fisher's exact test for biased strand distribution p-value = 3.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACAGCC  >  NC_000913/3424515‑3424796
                                                                                                                                                  |                                                                                                                                       
ctTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCa                                                                                                                                        >  8:367551/1‑148 (MQ=32)
  ttAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTTAAGGGTGGTATTTCCAGGTCGGCTCCATGCATCCTGGCGTCCACACTTCTAAGCCTCCCAcc                                                                                                                                      >  2:163356/1‑148 (MQ=255)
  ttAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCACGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTcccccc                                                                                                                                      >  6:439757/1‑148 (MQ=17)
   tAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCAcc                                                                                                                                      >  2:180998/1‑147 (MQ=32)
   tAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCAcc                                                                                                                                      <  6:259477/147‑1 (MQ=32)
   tAGGAGGAGACCGCCCCAGTCAAAATACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCACCATGCAGACTGGCGTACACACTTCTAAGCCTCCCAcc                                                                                                                                      >  6:348786/1‑147 (MQ=12)
        ggagACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATcc                                                                                                                                 >  3:417439/1‑147 (MQ=32)
         gagaCCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTa                                                                                                                               >  8:165693/1‑148 (MQ=32)
          agaCCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTac                                                                                                                              <  7:215331/148‑1 (MQ=32)
                ccccAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCa                                                                                                                        <  3:440840/148‑1 (MQ=32)
                 cccAGTCAAACTACCCACCAGACACTGTCCGCAACCCTGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCaa                                                                                                                       <  1:387652/148‑1 (MQ=17)
                 cccAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCaa                                                                                                                       <  5:417808/148‑1 (MQ=32)
                   cAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAgg                                                                                                                     >  8:102322/1‑148 (MQ=32)
                       cAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGcgc                                                                                                                  >  4:1706/1‑145 (MQ=17)
                          aCTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATg                                                                                                              >  7:401418/1‑148 (MQ=32)
                              cccACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTc                                                                                                           <  3:379249/147‑1 (MQ=32)
                               ccaccaGACACTGTCCGCAACCCGGATTACGGGTGAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCTGCTCCATGCAGACTGGCGTCGACACTTCTATGCCTCCCACCTATCCTACTCATCATGGCTCAATTTTCAg                                                                                                         >  1:387699/1‑148 (MQ=11)
                                  ccaGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAgtgt                                                                                                      >  4:286892/1‑148 (MQ=32)
                                   caGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAgtgt                                                                                                      >  6:210157/1‑147 (MQ=32)
                                   caGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAgtgt                                                                                                      >  2:137524/1‑147 (MQ=32)
                                    aGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTc                                                                                                     >  8:382775/1‑147 (MQ=32)
                                      acacTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCa                                                                                                                        <  5:60531/126‑1 (MQ=32)
                                      acacTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCa                                                                                                                        >  6:60531/1‑126 (MQ=32)
                                        acTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCt                                                                                                <  5:210157/148‑1 (MQ=32)
                                         cTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTTTTTTTTTAATTTTTATGCGTCCAACGTTTTCTACACATCAAGGCTCAATGTTCAGTGTCAAGCta                                                                                               <  8:167004/148‑1 (MQ=11)
                                         cTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCta                                                                                               >  1:196794/1‑148 (MQ=32)
                                           gTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCta                                                                                               >  7:42204/1‑146 (MQ=32)
                                            tCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCtat                                                                                              <  3:152166/146‑1 (MQ=32)
                                            tCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCtat                                                                                              <  8:401418/146‑1 (MQ=32)
                                             ccGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCCGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAg                                                                                            >  7:336023/1‑147 (MQ=17)
                                             ccGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACGCATCAAGGCTCAATGTTCAGTGTCAAGCTATAg                                                                                            >  3:299523/1‑147 (MQ=17)
                                               gCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGCCTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTaa                                                                                         <  8:336023/148‑1 (MQ=17)
                                               gCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACGCATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTaa                                                                                         <  4:299523/148‑1 (MQ=17)
                                                cAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCAACCTATCATACACATCAAGGCTCAATGTTCAGTTTCAAGCTATAGTa                                                                                          >  3:404608/1‑146 (MQ=12)
                                                  aCCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAgg                                                                                      <  7:382775/148‑1 (MQ=32)
                                                    ccGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGt                                                                                     >  8:445621/1‑147 (MQ=32)
                                                      ggATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCATGGTCGGCTCCATGCGGACTGGAGTCCACACTTCTAAGCCTCCCACCTATTCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCa                                                                                  <  2:357428/148‑1 (MQ=255)
                                                      ggATTACGGGTCAACGTTAGAACATAAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGAATACTTGCGTCCACACTTCTAAGACTCCCACCt                                                                                                                                     >  3:300511/1‑97 (MQ=11)
                                                       gATTACGGGTCAACGTTAGAACATCAATCATTAAAGGGTGGTATTTCCAGTTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACAAATCAATGCTCAATTTTCAGTGTCAAGCTATAGAAAAGGATccc                                                                                 >  1:96357/1‑146 (MQ=11)
                                                            cGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACgggg                                                                             <  5:368158/147‑1 (MQ=32)
                                                             gggTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTc                                                                           >  4:3502/1‑148 (MQ=32)
                                                                tCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCttt                                                                        >  8:10247/1‑148 (MQ=32)
                                                                 cAACGTTAGAACATCAAACATTAAAGGGTGGTTTTTCAAGGTCGGCTCCATTCAGACTGGCGTAAACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCttta                                                                       >  5:112352/1‑147 (MQ=255)
                                                                  aaCGTTAGAACATCAAACATTAAGGGGTGGTATTTCAAGGTCGTCTCAATGCAGATTTTTTTTTTAATTTTTATGCGTCCAACGTTTTCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTc                                                                       <  8:58690/147‑1 (MQ=2)
                                                                   aCGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCg                                                                     >  2:400101/1‑148 (MQ=32)
                                                                   aCGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCAACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCg                                                                     <  2:196794/148‑1 (MQ=17)
                                                                    cGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGt                                                                    <  5:449474/148‑1 (MQ=32)
                                                                           aCATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTATAGGTTCACGGGGTCTTTCCGTCTTGCcg                                                             >  3:231532/1‑148 (MQ=17)
                                                                             aTCAAACATTAAAGGGTGGTATTTCAATGTCGGCTCCTTGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTTTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCcgcg                                                           <  5:348786/148‑1 (MQ=12)
                                                                              tCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTg                                                                >  3:130536/1‑142 (MQ=32)
                                                                              tCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTg                                                                <  4:130536/142‑1 (MQ=32)
                                                                              tCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCgg                                                          >  5:357976/1‑148 (MQ=32)
                                                                               cAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACggg                                                                              >  1:327262/1‑127 (MQ=32)
                                                                                  aCATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCg                                                                     <  5:275857/133‑1 (MQ=32)
                                                                                  aCATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCAACTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCg                                                                     >  6:275857/1‑133 (MQ=17)
                                                                                     ttAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACt                                                   >  3:28818/1‑148 (MQ=32)
                                                                                        aaGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTTTATTATAAATTTTTATGCCTCCAACCTTTTCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGc                                                 <  6:190446/147‑1 (MQ=11)
                                                                                        aaGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCa                                                <  4:28226/148‑1 (MQ=32)
                                                                                         aGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCAt                                               <  1:400101/148‑1 (MQ=32)
                                                                                          gggTGTTATTTCAAGGGCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATc                                              <  1:78652/148‑1 (MQ=17)
                                                                                              ggtATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCACGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACCCTGCATCTTca                                          >  4:91456/1‑148 (MQ=17)
                                                                                               gtATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACCTCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTca                                          <  3:1706/147‑1 (MQ=17)
                                                                                                 aTTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAg                                       >  7:385424/1‑148 (MQ=32)
                                                                                                  tttCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGc                                      <  6:357976/148‑1 (MQ=32)
                                                                                                   ttCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTATGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTac                                                      <  8:89957/131‑1 (MQ=17)
                                                                                                    tCAAGGTCGGCTCCATTCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCg                                     <  7:466317/147‑1 (MQ=17)
                                                                                                    tCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCa                                                                                                          <  7:357366/78‑1 (MQ=34)
                                                                                                      aaGGTCGTCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATTGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGt                                  <  1:127808/148‑1 (MQ=17)
                                                                                                      aaGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGt                                  >  1:167484/1‑148 (MQ=32)
                                                                                                            ggCTACATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCTAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAAtt                            <  1:137524/148‑1 (MQ=17)
                                                                                                             gCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACgg                                                                               <  3:251310/96‑1 (MQ=33)
                                                                                                             gCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACgg                                                                               >  4:251310/1‑96 (MQ=33)
                                                                                                              ctcgaggCAGATTGTTGTACACAATTCTAAGCGACCAACCTATTCTACATATCAAGGCTCAATGTTCAGTGTCAAGGTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTc                          <  2:381332/142‑1 (MQ=11)
                                                                                                              cTCCCTGCCGCCTGGCGGCCACACTTCTAAGCCGCCCCCCTATCCTACACATCAAGGGGCAATGGGCCGGGGCAAGCGATAGTAAAGGGTCCCGGGGGCTTTCCCGCTTGCCGCGGGTACACTGCAGCTTCACAGCGAGTTCAATTTc                          <  3:165506/148‑1 (MQ=2)
                                                                                                              cTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTc                          <  7:58543/148‑1 (MQ=32)
                                                                                                              cTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTc                          <  7:10247/148‑1 (MQ=32)
                                                                                                                ccATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTc                                                                                   <  7:431065/89‑1 (MQ=33)
                                                                                                                 aaTGCAGACTGGCGTCCACAGTTATAAGCCTCCCACCTATTCTACTCATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCAc                        <  2:96357/146‑1 (MQ=255)
                                                                                                                   tGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCCCGGGGTCTTTCCGTCTTGCCGCGGGTCCACTGCATCTTCACAGCGAGTTCAATTTCACt                       >  6:37761/1‑146 (MQ=17)
                                                                                                                   tGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGa                     >  2:58396/1‑148 (MQ=32)
                                                                                                                    gCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAg                    >  2:66102/1‑148 (MQ=32)
                                                                                                                        aCTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACGGCGAGTTCAAttt                           <  7:402324/137‑1 (MQ=17)
                                                                                                                        aCTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACGGCGAGTTCAAttt                           >  8:402324/1‑137 (MQ=17)
                                                                                                                           ggCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTc                                                                   <  5:298123/94‑1 (MQ=33)
                                                                                                                           ggCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCaa                              >  5:200922/1‑131 (MQ=32)
                                                                                                                            gCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGt            <  5:439757/148‑1 (MQ=32)
                                                                                                                            gCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCATCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCgg              <  3:3709/146‑1 (MQ=17)
                                                                                                                             cGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTc                                                                   >  7:275620/1‑92 (MQ=33)
                                                                                                                             cGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTc                                                                   <  8:275620/92‑1 (MQ=33)
                                                                                                                             cGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTg                      >  7:434430/1‑137 (MQ=32)
                                                                                                                             cGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTg                      <  8:434430/137‑1 (MQ=32)
                                                                                                                              gTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATTTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTgg          <  7:25534/148‑1 (MQ=17)
                                                                                                                                ccACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGt            >  5:64501/1‑144 (MQ=32)
                                                                                                                                ccACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTg           >  5:222064/1‑145 (MQ=32)
                                                                                                                                ccACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGCGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTg           >  2:130290/1‑145 (MQ=17)
                                                                                                                                     cTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACAGc   >  6:303202/1‑148 (MQ=32)
                                                                                                                                      ttCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACAGcc  <  8:42204/148‑1 (MQ=32)
                                                                                                                                       tCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAACTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTc                          >  8:118056/1‑123 (MQ=17)
                                                                                                                                       tCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAACTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTc                          <  7:118056/123‑1 (MQ=17)
                                                                                                                                         tAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGa                                    >  8:264849/1‑111 (MQ=11)
                                                                                                                                                  |                                                                                                                                       
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACAGCC  >  NC_000913/3424515‑3424796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: