Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,695,037 C→A 5.3% Q169H (CAG→CAT purL ← phosphoribosylformyl‑glycineamide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,695,0370CA 5.3% 287.2 / 2.7 86Q169H (CAG→CATpurLphosphoribosylformyl‑glycineamide synthetase
Reads supporting (aligned to +/- strand):  ref base C (42/38);  new base A (3/2);  total (45/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCTCGGAGTTCGCCTGGGCAAACATATACAGTTCGATGTCGTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATT  >  NC_000913/2694896‑2695183
                                                                                                                                             |                                                                                                                                                  
gTGCTCGGAGTTCGCCTGGGCAAACATATACAGTTCGATGTCGTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACgg                                                                                                                                              <  3:152374/148‑1 (MQ=255)
     cGGAGTTCGCCTGGGCAAACATATACAGTTCGATGTCGTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATCTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTg                                                                                                                                         >  1:276232/1‑148 (MQ=255)
       gAGTTCGCCTGGGCAAACATATACAGTTCGATGTCGTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGcc                                                                                                                                       <  8:162808/148‑1 (MQ=255)
         gTTCGCCTGGGCAAACATATACAGTTCGATGTCGTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGcccc                                                                                                                                     <  7:12229/148‑1 (MQ=255)
          ttCGCCTGGGCAAACATATACTGTTCGATGTCGTTCGGGTTTCGACCAAGCTTTGTGAAAGCATCCTGCAGATAGGCAATTTCATCTTCCGCCAGAGCCAAGGCAAGACGCAGGTTAGCGGCGATCAGCGCCTGACGGCCCTGcccc                                                                                                                                     <  2:118937/147‑1 (MQ=255)
          ttCGCCTGGGCAAACATATACAGTTCGATGTCGTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATCTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCa                                                                                                                                    <  2:276232/148‑1 (MQ=255)
           tCGCCTGGGCAAACATATACAGTTCGATGTCGTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTg                                                                                                                                                  >  4:409651/1‑133 (MQ=255)
           tCGCCTGGGCAAACATATACAGTTCGATGTCGTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTg                                                                                                                                                  <  3:409651/133‑1 (MQ=255)
           tCGCCTGGGCAAACATATACAGTTCGATGTCGTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAg                                                                                                                                   >  6:421315/1‑148 (MQ=255)
           tCGCCTGGGCAAACATATACAGTTCGATGTCGTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCATGACGGCCCTGCCCCAg                                                                                                                                   >  2:350293/1‑148 (MQ=255)
           tCGCCTGGGCAAACATATACAGTTCGATGTCGTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCATGACGGCCCTGCCCCAg                                                                                                                                   >  2:344032/1‑148 (MQ=255)
                       aCATATACAGTTCGATGTCGTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCt                                                                                                                       >  1:266368/1‑148 (MQ=255)
                        cATATACAGTTCGATGTCGTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTg                                                                                                                      >  2:462171/1‑148 (MQ=255)
                          tataCAGTTCGATGTCGTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTgg                                                                                                                     >  1:442195/1‑147 (MQ=255)
                           ataCAGTTCGATGTCGTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGt                                                                                                                    >  5:237795/1‑147 (MQ=255)
                              cAGTTCGATGTCTTTCGTGTTACGACCTAGCTTTGTTAAATCATCCTGCTGATATTCAATTTCATCTTCCGCCAGAGCGAAGCCAAGTCGCAGGTTAGCGTCGATCAGCGCGTGACGGCCCTGGCCCAGCAAATGATCTCTGGTATcc                                                                                                                <  2:227687/148‑1 (MQ=255)
                                  tCGATGTCGTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAg                                                                                                            >  3:305709/1‑148 (MQ=255)
                                    gATGTCGTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTc                                                                                                          <  3:127607/148‑1 (MQ=255)
                                     aTGTCGTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCg                                                                                                         >  1:436042/1‑148 (MQ=255)
                                     aTGTCGTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCg                                                                                                         <  5:218127/148‑1 (MQ=255)
                                       gTCGTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGt                                                                                                       >  6:181254/1‑148 (MQ=255)
                                          gTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTg                                                                                                     <  5:430992/147‑1 (MQ=255)
                                          gTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGa                                                                                                    >  2:402082/1‑148 (MQ=255)
                                           ttCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGAt                                                                                                   >  2:312593/1‑148 (MQ=255)
                                           ttCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCATGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGAt                                                                                                   <  1:350293/148‑1 (MQ=255)
                                           ttCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCATGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGAt                                                                                                   <  1:344032/148‑1 (MQ=255)
                                            tCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGt                                                                                                           >  2:241426/1‑139 (MQ=255)
                                            tCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGt                                                                                                           <  1:241426/139‑1 (MQ=255)
                                             cGGTTTACGAGCTATCTTTTTGAAAGCATGCTGCATATAGTCAATTTGATCTTCCTCCAGAGCCATCGCACGACGCAGTATAGCGTCGATCAGCGCCTGAGGGCCCTGCCCCAGCTTATCAACGCTGGTAACCGGAGTCGGTTGAtg                                                                                                  <  2:94898/147‑1 (MQ=255)
                                             cGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGAtg                                                                                                  <  5:421315/147‑1 (MQ=255)
                                                gTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGAtggggg                                                                                              <  2:300873/148‑1 (MQ=255)
                                                       ccAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAaaca                                                                                       <  1:402082/148‑1 (MQ=255)
                                                        cAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACGGGAGTCGGTGGATGGTGGGCAaacaa                                                                                      >  6:206275/1‑148 (MQ=255)
                                                         aaGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGCGTCGGTAGATGGTGGGCAaacaac                                                                                     >  6:457056/1‑148 (MQ=255)
                                                              ttGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGctc                                                                                >  3:251737/1‑148 (MQ=255)
                                                               tgtgAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGctct                                                                               >  5:293992/1‑148 (MQ=255)
                                                                cggAAACCACCCTGCAGATACTCAATATCATCTGCCGCCAGCGCCAAGCCAAGACGCAGGGTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCACCGCTGGTAACCGGAGGCGGCTGATGGTGGGCAACCACCTGctct                                                                               <  4:52589/145‑1 (MQ=255)
                                                                   aaaGCATACTGCAGATAGTCAATTTCATCTTACGCCAGAGCCAAGCCAAGACGCAGGTAAGCGTCGATCAGCGCATGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGAAAACAACTTCTCGGcat                                                                           >  3:398134/1‑148 (MQ=255)
                                                                    aaGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTg                                                                                                                                                  >  6:32618/1‑76 (MQ=255)
                                                                    aaGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTg                                                                                                                                                  <  5:32618/76‑1 (MQ=255)
                                                                          ccTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCa                                                                                                                            <  2:262465/92‑1 (MQ=255)
                                                                          ccTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCa                                                                                                                            >  1:262465/1‑92 (MQ=255)
                                                                          ccTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTg                                                                                                     <  6:242118/115‑1 (MQ=255)
                                                                          ccTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTg                                                                                                     >  5:242118/1‑115 (MQ=255)
                                                                          ccTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTa                                                                     >  3:362364/1‑147 (MQ=255)
                                                                           cTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTaa                                                                    <  7:350002/147‑1 (MQ=255)
                                                                           cTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTaa                                                                    <  7:37210/147‑1 (MQ=255)
                                                                            tGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGa                                                                                                    >  2:400582/1‑114 (MQ=255)
                                                                            tGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGa                                                                                                    <  1:400582/114‑1 (MQ=255)
                                                                                 aTAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGAAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCaaaa                                                             <  5:206275/148‑1 (MQ=255)
                                                                                    gTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAg                                                          <  6:293992/148‑1 (MQ=255)
                                                                                      cAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGAc                                                        >  1:440412/1‑148 (MQ=255)
                                                                                        aTTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCg                                                      >  8:478780/1‑148 (MQ=255)
                                                                                        aTTTCATCTTCAGCCAGATCCAATCAAAGACGCAGGTTAGCGTCGATAAGCGCCTGACGGCCCTGACCCAGAAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCg                                                      >  1:63428/1‑148 (MQ=255)
                                                                                          ttCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCGAAAGCAAAAAAGACCGtt                                                    >  1:178694/1‑148 (MQ=255)
                                                                                            cATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGttt                                                   <  6:237795/147‑1 (MQ=255)
                                                                                             aTCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTcc                                                 <  4:305709/148‑1 (MQ=255)
                                                                                             aTCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTc                                                  >  6:172544/1‑147 (MQ=255)
                                                                                             aTCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTc                                                  <  4:251737/147‑1 (MQ=255)
                                                                                                ttCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCcat                                               >  8:363651/1‑147 (MQ=255)
                                                                                                  ccGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCcatca                                             <  1:462171/147‑1 (MQ=255)
                                                                                                  ccGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCcat                                               <  2:266368/145‑1 (MQ=255)
                                                                                                  ccGCAAGATCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGACCCAGCAAATCAACGCTGGTAACAGGAGTCCGTTGATGGTGGGCCAACAACTCCTCTGCATCAACTAAAGCAAAAAAGACCGTTTCcagca                                             >  6:4252/1‑147 (MQ=255)
                                                                                                       agagCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGt                                       <  2:442195/148‑1 (MQ=255)
                                                                                                       agagCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATCCGGt                                       >  7:94216/1‑148 (MQ=255)
                                                                                                            caagccaagACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGc                                  <  5:181254/148‑1 (MQ=255)
                                                                                                                  aagACGCAGGGTAGCGGCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCa                            <  7:44071/148‑1 (MQ=255)
                                                                                                                   agACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAg                           >  8:314890/1‑148 (MQ=255)
                                                                                                                      cGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCgg                        >  1:344551/1‑148 (MQ=255)
                                                                                                                        catgTTAGCGTCGATCATCGCCTGACGTCACTGCCAAAGCAAATCAAAGCTGGTAACCTGAGTCGGTTGATGGTTGTCAAACAACTGCTCTGCATCATCTAAATCAAAAAAGACCTTTTCCATCATGCGGTCGTGCAGTTCATCGGTa                      <  2:63428/145‑1 (MQ=255)
                                                                                                                        cAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGt                       >  6:155028/1‑147 (MQ=255)
                                                                                                                        cAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGt                       >  8:2686/1‑147 (MQ=255)
                                                                                                                           gTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGc                                                                             <  2:464287/90‑1 (MQ=255)
                                                                                                                           gTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGc                                                                             >  1:464287/1‑90 (MQ=255)
                                                                                                                           gTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAAcc                   >  2:137708/1‑148 (MQ=255)
                                                                                                                            ttAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACct                  <  7:316594/148‑1 (MQ=255)
                                                                                                                            ttAGCGGCGATCAGCGCCTGACGTCCCTGCCCCAGCAAATCAACGGTGTTATcctt                                                                                                              <  8:338363/56‑3 (MQ=255)
                                                                                                                             tAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGa                                                                                                             >  7:338363/1‑56 (MQ=255)
                                                                                                                                  tCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGcc            <  1:137708/148‑1 (MQ=255)
                                                                                                                                   cGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGCCCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCa           >  7:348770/1‑148 (MQ=255)
                                                                                                                                   cGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCa           >  7:85622/1‑148 (MQ=255)
                                                                                                                                   cGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCa           >  2:226693/1‑148 (MQ=255)
                                                                                                                                      tCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGTTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTg        >  8:21368/1‑148 (MQ=255)
                                                                                                                                      tCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCcatc                                              >  3:286534/1‑110 (MQ=255)
                                                                                                                                      tCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCcatc                                              <  4:286534/110‑1 (MQ=255)
                                                                                                                                          cgcCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCa    <  7:21368/148‑1 (MQ=255)
                                                                                                                                            ccTGACGGCCCTGCCCCAGCAAATCCACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCAtt  <  8:348770/148‑1 (MQ=255)
                                                                                                                                            ccTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCAtt  <  7:363651/148‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
GTGCTCGGAGTTCGCCTGGGCAAACATATACAGTTCGATGTCGTTCGGGTTACGACCAAGCTTTGTGAAAGCATCCTGCAGATAGTCAATTTCATCTTCCGCCAGAGCCAAGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATT  >  NC_000913/2694896‑2695183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: