Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,278,883 G→T 5.1% A293E (GCA→GAA)  bcr ← bicyclomycin/cysteine/sulfonamide efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,278,8830GT 5.1% 304.5 / 2.9 88A293E (GCA→GAA) bcrbicyclomycin/cysteine/sulfonamide efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (50/33);  new base T (2/3);  total (52/36)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

CGCCATATGGGGAAACTCATCAAGAATGACCGCCATCGCATTGGATGACACCATCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCGCGACGTGGT  >  NC_000913/2278744‑2279030
                                                                                                                                           |                                                                                                                                                   
cGCCATATGGGGAAACTAATCAAGAATGACCGCCATCGCATTGGATGACACCATCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCataata                                                                                                                                             <  1:44535/148‑1 (MQ=255)
   cATATGGGGAAACTCATCAAGAATGACCGCCATCGCATTGGATGACACCATCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAAt                                                                                                                                          <  6:168425/148‑1 (MQ=255)
   cATATGGGGAAACTCATCAAGAATGACCGCCATCGCATTGGATGAAACCATCGACACGCAGCCAACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAAt                                                                                                                                          <  7:192822/148‑1 (MQ=255)
      atGGGGAAACTCATCAAGAATGACCGCCATCGCATTGGATGACACCATCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCCGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTg                                                                                                                                        >  3:379181/1‑147 (MQ=255)
      atGGGGAAACTCATCAAGAATGACCGCCATCGCATTGGATGACACCATCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTg                                                                                                                                        >  7:44467/1‑147 (MQ=255)
       tGGGGAAACTCATCAAGAATGACCGCCATCGCATTGGATGACACCATCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGt                                                                                                                                       <  4:345627/147‑1 (MQ=255)
        ggggAAACTCATCAAGAATGACCGCCATCGCATTGGATGACACCATCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTAt                                                                                                                                     >  8:129384/1‑148 (MQ=255)
             aaCTCATCAAGAATGACCGCCATCGCATTGGATGACACCATCGACACGCTGCCCACAATCGCCGGAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGGACTGATGACCATCCACGCTGCCATAATAATTTGTATCcaca                                                                                                                                <  2:113980/148‑1 (MQ=255)
               cTCATCAAGAATGACCGCCATCGCATTGGATGACACCATCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAAc                                                                                                                              >  1:416059/1‑148 (MQ=255)
                 catcaAGAATGACCGCCATCGCATTGGATGACACCATCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAAccc                                                                                                                            >  8:98752/1‑148 (MQ=255)
                            aCCGCCATCGCATTGGATGACACCATCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACa                                                                                                                 >  4:94957/1‑148 (MQ=255)
                             ccGCCATCGCATTGGATGACACCATCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACat                                                                                                                <  8:464691/148‑1 (MQ=255)
                                   tCGCATTGGATGACACCATCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTaa                                                                                                          >  6:58065/1‑148 (MQ=255)
                                    cGCATTGGATGACACCATCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGACCATATTTAAc                                                                                                         >  3:120899/1‑148 (MQ=255)
                                    cGCATTGGATGACACCATCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAAc                                                                                                         >  3:88589/1‑148 (MQ=255)
                                       aTTGGATGACACCATCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAAcgcg                                                                                                      <  7:129384/148‑1 (MQ=255)
                                       aTTGGATGACACCATCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAAcgcg                                                                                                      <  2:416059/148‑1 (MQ=255)
                                           gATGACACCATCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCAACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCaa                                                                                                  >  8:255293/1‑148 (MQ=255)
                                                 acCATCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATgcggc                                                                                            >  3:296765/1‑148 (MQ=255)
                                                  ccATCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGa                                                                                                                    <  2:243684/123‑1 (MQ=255)
                                                  ccATCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGa                                                                                                                    >  1:243684/1‑123 (MQ=255)
                                                    aTCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGa                                                                                         <  8:337292/148‑1 (MQ=255)
                                                     tCGACACGCAGCCCACAAACGCCGCAACGCCAGCCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGAc                                                                                        >  4:267694/1‑148 (MQ=255)
                                                         cacGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAg                                                                                    >  3:267867/1‑148 (MQ=255)
                                                            gCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCgg                                                                                 >  1:164401/1‑148 (MQ=255)
                                                             cAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCACACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGc                                                                                >  4:284468/1‑148 (MQ=255)
                                                                cccACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGt                                                                             >  7:146977/1‑148 (MQ=255)
                                                                   acaAACGCCGCAACGCCAACCACCGGCGACCAAAATCCCAGCCCCCGCAGCGCACTGATGACCCTCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTa                                                                           >  4:94005/1‑147 (MQ=255)
                                                                    caAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTa                                                                           >  4:386238/1‑146 (MQ=255)
                                                                    caAACGCCGAAACGCCAACCACCAGCGACCAACATCCCAGCCCCAGCAGCCCACTTATGACCATCAACGCTGACAAAATAAATTAAATCCACAACCACTACCGGAACATATTTAACGAGCCAATACAGACGACGAAGCGGCTGATa                                                                           >  4:15887/1‑146 (MQ=255)
                                                                     aaaCGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTa                                                                           >  6:68023/1‑145 (MQ=255)
                                                                     aaaCGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAg                                                                        <  5:68023/148‑1 (MQ=255)
                                                                     aaaCGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAg                                                                        <  4:379181/148‑1 (MQ=255)
                                                                        cgccgcAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATg                                                                     <  7:98752/148‑1 (MQ=255)
                                                                          ccgcAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGt                                                                   >  5:21404/1‑148 (MQ=255)
                                                                          ccgcAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGt                                                                   >  5:106541/1‑148 (MQ=255)
                                                                           cgcAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGtc                                                                  <  2:47423/148‑1 (MQ=255)
                                                                           cgcAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGt                                                                   >  4:224732/1‑147 (MQ=255)
                                                                             cAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAAcc                                                                                                                             <  7:322499/87‑1 (MQ=255)
                                                                             cAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAAcc                                                                                                                             >  8:322499/1‑87 (MQ=255)
                                                                               aCGCCAACCACCAGCGACCAAACTCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACACCCCCGAGCGGACCATATTTAACGCGCCAATGCGGCGGACGCAGCGGCTGTTAAAGATGGtgatca                                                              >  1:152308/1‑148 (MQ=255)
                                                                                cGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCAc                                                             <  5:58065/148‑1 (MQ=255)
                                                                                  ccAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACg                                                            >  5:8632/1‑147 (MQ=255)
                                                                                  ccAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGa                                                           <  3:94957/148‑1 (MQ=255)
                                                                                    aaCCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCCCGaa                                                          >  8:402646/1‑147 (MQ=255)
                                                                                    aaCCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGaa                                                          >  2:110112/1‑147 (MQ=255)
                                                                                     accaccAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCa                                                                                                   <  3:52718/105‑1 (MQ=255)
                                                                                     accaccAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCa                                                                                                   >  4:52718/1‑105 (MQ=255)
                                                                                     accaccAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGc                                                                                >  6:227643/1‑124 (MQ=255)
                                                                                     accaccAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGc                                                                                <  5:227643/124‑1 (MQ=255)
                                                                                     accaccAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGaa                                                          >  7:137093/1‑146 (MQ=255)
                                                                                     accaccAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACa                                                        <  2:164401/148‑1 (MQ=255)
                                                                                          caGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGaaa                                                    >  4:258243/1‑147 (MQ=255)
                                                                                           aGCGACCAAAATCCCAGCACCAGCAGCGCACTGATGACAATCCAGGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCACTGCGGCGTACGAAGCGGCTGTTAAAGAGGGTCATGACGCACAGaaaaa                                                  >  8:434194/1‑148 (MQ=255)
                                                                                              gACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTg                                                                              <  2:42148/117‑1 (MQ=255)
                                                                                              gACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTg                                                                              >  1:42148/1‑117 (MQ=255)
                                                                                                ccAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTa                                                                                                           >  2:397578/1‑86 (MQ=255)
                                                                                                ccAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTa                                                                                                           <  1:397578/86‑1 (MQ=255)
                                                                                                  aaaaTCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGGTAAAGATGGTCATCACGAACAGAAAAACAATGtt                                           >  6:166728/1‑148 (MQ=255)
                                                                                                   aaaTCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGttt                                          <  3:267694/148‑1 (MQ=255)
                                                                                                   aaaTCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGttt                                          <  8:146977/148‑1 (MQ=255)
                                                                                                   aaaTCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGtt                                           >  4:372848/1‑147 (MQ=255)
                                                                                                          agccccagcAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCaa                                   <  8:137093/148‑1 (MQ=255)
                                                                                                          agccccagcAGCGAACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCaa                                   <  2:336950/148‑1 (MQ=255)
                                                                                                              ccagcAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGta                               <  6:201650/148‑1 (MQ=255)
                                                                                                               cagcagCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGtaa                              <  3:224732/148‑1 (MQ=255)
                                                                                                               cagcagCACACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGtaa                              <  3:284468/148‑1 (MQ=255)
                                                                                                                agcagcGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGtaat                             <  8:286903/148‑1 (MQ=255)
                                                                                                                 gcagcGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGtaata                            <  4:296765/148‑1 (MQ=255)
                                                                                                                 gcagcGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGtaat                             >  5:163504/1‑147 (MQ=255)
                                                                                                                  cagcGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACCACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATAACGAACAGAAAAACAATGTTTAGCGCAAAGtaataa                           >  1:293906/1‑148 (MQ=255)
                                                                                                                  cagcGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGtaataa                           >  8:227715/1‑148 (MQ=255)
                                                                                                                   agcGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAAc                          >  7:415968/1‑148 (MQ=255)
                                                                                                                      gcACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCaa                       <  6:163504/148‑1 (MQ=255)
                                                                                                                           gatgaCCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCaa                                   <  4:77399/131‑1 (MQ=255)
                                                                                                                           gatgaCCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCaa                                   >  3:77399/1‑131 (MQ=255)
                                                                                                                           gatgaCCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGttt                  >  4:94125/1‑148 (MQ=255)
                                                                                                                           gatgaCCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGttt                  >  3:174776/1‑148 (MQ=255)
                                                                                                                           gatgaCCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGttt                  >  3:93432/1‑148 (MQ=255)
                                                                                                                           gatgaCCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGttt                  >  1:36784/1‑148 (MQ=255)
                                                                                                                             tgaCCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTc                >  7:215693/1‑148 (MQ=255)
                                                                                                                              gaCCATCCACTCTTCCATAATAATATGTATCCACAACCCCGAGCGGAACATATTTAACTCGCCAATGCGGCTGACGAATCGTCTGTTAATGATTGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTc                <  8:44467/147‑1 (MQ=255)
                                                                                                                                 cATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGATCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGgc            >  3:420183/1‑148 (MQ=255)
                                                                                                                                    ccACGCTTCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGtaa                              <  2:113471/127‑1 (MQ=255)
                                                                                                                                    ccACGCTTCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGtaa                              <  2:107380/127‑1 (MQ=255)
                                                                                                                                    ccACGCTTCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGtaa                              >  1:107380/1‑127 (MQ=255)
                                                                                                                                    ccACGCTTCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGtaa                              >  1:113471/1‑127 (MQ=255)
                                                                                                                                      aCGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCTCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATGACGAACAGAAAAAAAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCGCGAc       >  4:300525/1‑148 (MQ=255)
                                                                                                                                           gCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCGCGACGTGGt  >  5:184871/1‑148 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
CGCCATATGGGGAAACTCATCAAGAATGACCGCCATCGCATTGGATGACACCATCGACACGCAGCCCACAAACGCCGCAACGCCAACCACCAGCGACCAAAATCCCAGCCCCAGCAGCGCACTGATGACCATCCACGCTGCCATAATAAATTGTATCCACAACCCCGAGCGGAACATATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCGCGACGTGGT  >  NC_000913/2278744‑2279030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑