Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,553,553 T→C 6.1% N96D (AAC→GAC)  adhP ← ethanol‑active dehydrogenase/acetaldehyde‑active reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,553,5530TC 6.1% 199.0 / 3.5 65N96D (AAC→GAC) adhPethanol‑active dehydrogenase/acetaldehyde‑active reductase
Reads supporting (aligned to +/- strand):  ref base T (30/31);  new base C (2/2);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

GTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATC  >  NC_000913/1553408‑1553700
                                                                                                                                                 |                                                                                                                                                   
gTACTGCTGCTGGCAGCCGCCGAGTCCAGACCATCTTGAACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTAc                                                                                                                                                   <  4:66205/148‑1 (MQ=255)
gTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTAc                                                                                                                                                   <  1:192228/148‑1 (MQ=255)
gTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGAACTTTTACCGCGTAATCGGCTACCACGATGCACTCTTACGCCATCCCGCCATCAACGCTGTATCCGTAATTTTTAACTGAACTGCAGAGCGTTTAGTTACCACTTTTa                                                                                                                                                    >  8:142958/1‑147 (MQ=255)
 tAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACa                                                                                                                                                  <  7:268581/148‑1 (MQ=255)
  aaTGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAg                                                                                                                                                 >  3:249833/1‑148 (MQ=255)
    tgctgcTGGCCGCCGCCGAGTCCAGACCATCTGGCTCTTTTACCGCGTAATCGGCGACCACGATGCACTCTTGCGCCATCCCGCCATAAACGCTGTATCCGGCATTTTTAACTGAACGGCATAGCGTTTCGTTACCACTGTTACAGt                                                                                                                                                <  6:272899/147‑1 (MQ=255)
            gccgccgccGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCaa                                                                                                                                        <  8:374067/147‑1 (MQ=255)
            gccgccgccGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCaa                                                                                                                                        <  7:401400/147‑1 (MQ=255)
            gccgccgccGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCa                                                                                                                                         <  5:121350/146‑1 (MQ=255)
             ccgccgccgAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATg                                                                                                                                      <  5:145358/148‑1 (MQ=255)
                   ccgAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCa                                                                                                                                >  1:101088/1‑148 (MQ=255)
                          cAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTc                                                                                                                          <  3:98147/147‑1 (MQ=255)
                          cAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCg                                                                                                                         <  4:56035/148‑1 (MQ=255)
                             aCCATCTGGCACTTTTACCGCGTAATCGGTGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCg                                                                                                                         >  3:99801/1‑145 (MQ=255)
                              ccATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGa                                                                                                                     <  6:36275/148‑1 (MQ=255)
                                         ttttACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAAccacgccacg                                                                                                          >  8:295674/1‑148 (MQ=255)
                                          tttACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACcacgccacgc                                                                                                         >  3:434835/1‑148 (MQ=255)
                                          tttACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACcacgccacgc                                                                                                         >  1:125037/1‑148 (MQ=255)
                                              ccGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGc                                                                                                     <  4:434835/148‑1 (MQ=255)
                                                gcgTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCAc                                                                                                   >  7:327092/1‑148 (MQ=255)
                                                      tCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTAGTAGAACCACGCCACGCTGGCACGATCGc                                                                                             >  8:224487/1‑148 (MQ=255)
                                                       cGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAAccacgccacg                                                                                                          >  3:289332/1‑134 (MQ=255)
                                                       cGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAAccacgccacg                                                                                                          <  4:289332/134‑1 (MQ=255)
                                                        ggtgACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCt                                                                                           <  4:99801/145‑1 (MQ=255)
                                                        ggCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGcc                                                                                            <  7:185215/147‑1 (MQ=255)
                                                                  aTGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAAt                                                                                  >  6:396565/1‑147 (MQ=255)
                                                                  aTGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATg                                                                                 <  4:249833/148‑1 (MQ=255)
                                                                   tGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGa                                                                                <  7:142958/148‑1 (MQ=255)
                                                                     cACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAgg                                                                              >  3:22091/1‑148 (MQ=255)
                                                                     cACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAgg                                                                              <  3:70170/148‑1 (MQ=255)
                                                                         ctTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGac                                                                          >  4:23631/1‑148 (MQ=255)
                                                                          ttCCGCCATCCCGCAATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGaca                                                                         >  5:80871/1‑148 (MQ=255)
                                                                                  tcccgccatcAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTCACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGAcc                                                                 >  7:270667/1‑148 (MQ=255)
                                                                                   cccgccatcAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTg                                                                            >  6:377289/1‑136 (MQ=255)
                                                                                   cccgccatcAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTg                                                                            <  5:377289/136‑1 (MQ=255)
                                                                                    ccgccatcAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGAccc                                                                <  6:80871/147‑1 (MQ=255)
                                                                                     cgccatcAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCAAc                                                              >  2:207348/1‑148 (MQ=255)
                                                                                      gccatcAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCCTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAgg                                                                              >  4:376052/1‑131 (MQ=255)
                                                                                      gccatcAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCCTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAgg                                                                              <  3:376052/131‑1 (MQ=255)
                                                                                       ccatcAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTCACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACtt                                                            >  3:283297/1‑148 (MQ=255)
                                                                                        catcAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTCACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTc                                                           <  8:270667/148‑1 (MQ=255)
                                                                                           cAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGtttt                                                                                     <  4:151927/119‑1 (MQ=255)
                                                                                           cAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGtttt                                                                                     >  3:151927/1‑119 (MQ=255)
                                                                                           cAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTa                                                                                    >  4:312254/1‑120 (MQ=255)
                                                                                           cAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTa                                                                                    <  3:312254/120‑1 (MQ=255)
                                                                                           cAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGc                                                        >  1:451695/1‑148 (MQ=255)
                                                                                            aaCGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGcc                                                       <  2:125037/148‑1 (MQ=255)
                                                                                                  gTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTCCCCCTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTACTGAGGTGACACCTGGCCCCACTTCTGCCACcaca                                                 >  5:411774/1‑148 (MQ=255)
                                                                                                  gTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACcaca                                                 >  5:394132/1‑148 (MQ=255)
                                                                                                   tATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTCTTACAGTATACGCAATGACCGCATCCTTCGTAGAACCACGCCACGCt                                                                                                        >  6:56909/1‑92 (MQ=255)
                                                                                                     tCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCg                                              <  4:22091/148‑1 (MQ=255)
                                                                                                          cATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGcc                                         <  7:295674/148‑1 (MQ=255)
                                                                                                             ttttAACTGAACGGCAGAGCGTTTCTTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGTTTTTAATGAGGAGACACCTGGACCCACTTCTGCCACCACACCGATGCCtt                                       >  1:104476/1‑147 (MQ=255)
                                                                                                             ttttAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCtt                                       >  7:121004/1‑147 (MQ=255)
                                                                                                              tttAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTc                                      <  6:153484/147‑1 (MQ=255)
                                                                                                              tttAACTGAACGGCAGAGCGTTTCGTTACCACTGTCACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTc                                      <  4:283297/147‑1 (MQ=255)
                                                                                                                       aCGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAg                             >  8:214380/1‑147 (MQ=255)
                                                                                                                          gCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAAtt                         <  2:451695/148‑1 (MQ=255)
                                                                                                                                   ttCGTTACCACTGTTACAGTATTCGCAATGACTGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGtt                >  3:189782/1‑148 (MQ=255)
                                                                                                                                     cGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGttt               <  2:101088/147‑1 (MQ=255)
                                                                                                                                       ttACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGt            <  5:396565/148‑1 (MQ=255)
                                                                                                                                         aCCACTGTTACAGTATTCGCAAGGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTc           <  8:327092/147‑1 (MQ=255)
                                                                                                                                           cACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGCACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCAc         >  5:387257/1‑147 (MQ=255)
                                                                                                                                               gTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCaaaa    >  2:387430/1‑148 (MQ=255)
                                                                                                                                               gTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCaaa     >  1:32911/1‑147 (MQ=255)
                                                                                                                                                 tACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATc  >  7:52904/1‑148 (MQ=255)
                                                                                                                                                 tACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATc  >  3:220069/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATC  >  NC_000913/1553408‑1553700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑