Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	755,790	G→A	W77* (TGG→TGA)	sdhD →	succinate dehydrogenase, membrane subunit, binds cytochrome b556

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	755,790	0	G	A	100.0%	87.6 / NA	26	W77* (TGG→TGA)	sdhD	succinate dehydrogenase, membrane subunit, binds cytochrome b556
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/14);  total (12/14)

ATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGG  >  NC_000913/755647‑755899                                                                                                                                                |                                                                                                              atcatTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGgt                                                                                                          >  8:66715/1‑149 (MQ=255)             gTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCCGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGgtg                                                                                                         <  1:175620/138‑1 (MQ=255)             gTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCCGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGgtg                                                                                                         >  2:175620/1‑138 (MQ=255)                       tCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGTTCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTa                                                                                              <  1:117965/139‑1 (MQ=255)                       tCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGTTCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTa                                                                                              >  2:117965/1‑139 (MQ=255)                             ccAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGc                                                                               <  7:66715/148‑1 (MQ=255)                             ccAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCt                                                                              <  3:121950/149‑1 (MQ=255)                              cAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCtt                                                                             >  2:131285/1‑149 (MQ=255)                                gTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTg                                                                           >  2:19750/1‑149 (MQ=255)                                   gCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTgcgc                                                                        <  1:142996/149‑1 (MQ=255)                                        cTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGAt                                                                   >  3:105676/1‑149 (MQ=255)                                          gACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGgtg                                                                                                         >  4:83129/1‑109 (MQ=255)                                          gACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGgtg                                                                                                         <  3:83129/109‑1 (MQ=255)                                                             ggTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGt                                              <  4:105676/149‑1 (MQ=255)                                                                   ttcGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAAc                                                           <  1:37911/130‑1 (MQ=255)                                                                   ttcGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAAc                                                           >  2:37911/1‑130 (MQ=255)                                                                                    cAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAAc                                                           >  7:1903/1‑113 (MQ=255)                                                                                    cAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAAc                                                           <  8:1903/113‑1 (MQ=255)                                                                                              aCCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGtt                                             >  8:88700/1‑117 (MQ=255)                                                                                              aCCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGtt                                             <  7:88700/117‑1 (MQ=255)                                                                                                ccTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGAtgctg                                                               >  5:38093/1‑97 (MQ=255)                                                                                                ccTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGAtgctg                                                               <  6:38093/97‑1 (MQ=255)                                                                                                 ctgctgGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtgt          <  1:19750/149‑1 (MQ=255)                                                                                                         gcTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTggg  <  1:131285/149‑1 (MQ=255)                                                                                                                                 cTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtgtg         >  4:48459/1‑118 (MQ=255)                                                                                                                                 cTGGATCGGCATGTGACAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtgtg         <  3:48459/118‑1 (MQ=255)                                                                                                                                                |                                                                                                              ATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGG  >  NC_000913/755647‑755899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑