Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	446,437	C→A	R77L (CGC→CTC)	yajR ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	446,437	0	C	A	97.4%	252.7 / NA	76	R77L (CGC→CTC)	yajR	putative transporter
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (35/39);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACGTGGTCAGAACCGGCAGAACCAT  >  NC_000913/446301‑446579                                                                                                                                         |                                                                                                                                               cgcCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGt                                                                                                                                    <  7:79171/149‑1 (MQ=255)     aTAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCgg                                                                                                                                 >  5:268379/1‑148 (MQ=255)        aCGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGACCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGAGCAGCGATAAAGCTACCGGCGGCAAACACCGCCAGCCCACCGACTATTAATGGATTGAGACCAATGCGGTCGGaaa                                                                                                                              <  5:299942/148‑1 (MQ=255)         cggcggcggCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGATTAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATTACGCTACCGGCGGCAATCACCGCCAGCGCACCGACAATTAATGGGTTGAGACCAATGCGGGCGGAAAGc                                                                                                                            <  2:247105/149‑1 (MQ=255)                 gcAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCaaa                                                                                                                    <  6:150632/149‑1 (MQ=255)                       gCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAAt                                                                                                              >  1:67075/1‑149 (MQ=255)                        cTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATc                                                                                                             >  8:156953/1‑149 (MQ=255)                         tCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCt                                                                                                            >  1:307962/1‑149 (MQ=255)                             gagCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGaa                                                                                                         >  2:37977/1‑148 (MQ=255)                                tcTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGGCGGAAAGCAGGCCAAACGGAATCTGAAacac                                                                                                     >  5:135797/2‑149 (MQ=255)                                ccTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAAc                                                                                                     >  8:387902/1‑149 (MQ=255)                                    gTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGc                                                                                                  <  7:10154/148‑1 (MQ=255)                                    gTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGc                                                                                                  <  6:135797/148‑1 (MQ=255)                                    gTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGGCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGc                                                                                                  <  2:10663/148‑1 (MQ=255)                                      aGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGATAGCAGGCCAAACGGAATCTGAAAAACGGCCt                                                                                                >  2:153930/1‑148 (MQ=255)                                       gcgcCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGa                                                                                              <  4:337082/149‑1 (MQ=255)                                        cgcCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAg                                                                                             >  3:52904/1‑149 (MQ=255)                                          cccGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGt                                                                                            >  6:226110/1‑148 (MQ=255)                                           ccGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATTGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCa                                                                                          <  5:406646/149‑1 (MQ=255)                                           ccGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGt                                                                                            >  4:217466/1‑147 (MQ=255)                                             ggCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGTAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGa                                                                                        <  3:206988/149‑1 (MQ=255)                                                    aataatTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATaaa                                                                                 <  7:387902/149‑1 (MQ=255)                                                     ataatTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGGTGGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAAt                                                                                <  5:332774/149‑1 (MQ=255)                                                      taatTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATa                                                                               <  2:141128/149‑1 (MQ=255)                                                          tCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCaa                                                                           >  3:11576/1‑149 (MQ=255)                                                            cccAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAAt                                                                          <  6:309702/148‑1 (MQ=255)                                                            cccAGATGGAGTCGGAAAGAGCAGCGATAACGCTACCGGCGGCAAACACCGCAAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAAt                                                                          <  7:136234/148‑1 (MQ=255)                                                             ccAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAGGCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATgg                                                                        >  6:55000/1‑149 (MQ=255)                                                             ccAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATgg                                                                        >  5:392091/1‑149 (MQ=255)                                                             ccAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATgg                                                                        >  3:227199/1‑149 (MQ=255)                                                             ccAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGCCCTGAGTCAGACCATAAATACCAATgg                                                                        <  4:7981/149‑1 (MQ=255)                                                              cAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGc                                                                       <  3:217466/149‑1 (MQ=255)                                                                 aTGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAAt                                                                    <  5:226110/149‑1 (MQ=255)                                                                     aGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATAcc                                                                 >  2:254336/1‑148 (MQ=255)                                                                       tCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGAt                                                              <  5:129057/149‑1 (MQ=255)                                                                         ggAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGAtt                                                             >  6:249525/1‑148 (MQ=255)                                                                          gAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTaa                                                           <  6:329860/149‑1 (MQ=255)                                                                          gAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTaa                                                           >  5:384628/1‑149 (MQ=255)                                                                          gAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGACCTGAGTCAGACCATAAATACCAATGGCAATACCGATTaa                                                           <  7:156953/149‑1 (MQ=255)                                                                           aaaGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAAt                                                          >  2:190556/1‑149 (MQ=255)                                                                               cgcAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCtt                                                      <  4:85415/149‑1 (MQ=255)                                                                               cgcAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCtt                                                      >  8:174990/1‑149 (MQ=255)                                                                                gcAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTc                                                     >  2:46510/1‑149 (MQ=255)                                                                                 cAGCGAT‑ACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGc                                                   >  8:366057/1‑149 (MQ=255)                                                                                  aGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGc                                                   <  1:338685/149‑1 (MQ=255)                                                                                     gATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTgg                                                <  4:192784/149‑1 (MQ=255)                                                                                       tAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCa                                              >  3:135678/1‑149 (MQ=255)                                                                                         aCGCTACCGGCGGCAAACACCGCCAGCACACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCAc                                             <  4:267346/148‑1 (MQ=255)                                                                                           gCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCtt                                          >  2:202811/1‑149 (MQ=255)                                                                                             tACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTg                                         >  5:197194/1‑148 (MQ=255)                                                                                               ccGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCag                                      <  2:67075/149‑1 (MQ=255)                                                                                               ccGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCa                                       <  2:250148/148‑1 (MQ=255)                                                                                               ccGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCa                                       <  1:190556/148‑1 (MQ=255)                                                                                                   cggcAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGcc                                  <  6:268379/149‑1 (MQ=255)                                                                                                     gcAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCAt                                <  4:135678/149‑1 (MQ=255)                                                                                                     acAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCAt                                >  3:364924/2‑149 (MQ=255)                                                                                                      cAAACACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATg                               <  1:254336/149‑1 (MQ=255)                                                                                                       aaaCACCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGc                              >  3:291429/1‑149 (MQ=255)                                                                                                           acCGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTa                          >  1:79185/1‑149 (MQ=255)                                                                                                            ccGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATTCCGATTAATGCTTCGCTGGCCCCTTGCAGCGCCATGCCGTAc                         >  6:92150/1‑149 (MQ=255)                                                                                                             cGCCAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACg                        >  6:318072/1‑149 (MQ=255)                                                                                                               ccAGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACGTg                      <  4:227199/149‑1 (MQ=255)                                                                                                                 aGCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACGTGGt                    <  4:364924/149‑1 (MQ=255)                                                                                                                  gCCCACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACGTGGTc                   <  5:249525/149‑1 (MQ=255)                                                                                                                   cccACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACGTGGTCa                  <  1:37977/149‑1 (MQ=255)                                                                                                                     cACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACGTGGTCAGa                <  7:366057/149‑1 (MQ=255)                                                                                                                     cACCGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGACAGACCATAAATACCAATGGCCATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACGTGGTCAg                 >  5:174890/1‑148 (MQ=255)                                                                                                                       ccGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACGTGGTCAGAAc              >  6:263733/1‑149 (MQ=255)                                                                                                                        cGACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACGTGGTCAGAAcc             >  3:200990/1‑149 (MQ=255)                                                                                                                         gACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACGTGGTCAGAACCg            <  7:174990/149‑1 (MQ=255)                                                                                                                         gACAATTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCAGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACGTGGTCAGAACCg            >  6:181337/1‑149 (MQ=255)                                                                                                                            aaTTAATGGTTTGAGATCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACGTGGTc                   >  3:299310/1‑139 (MQ=255)                                                                                                                            aaTTAATGGTTTGAGATCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACGTGGTc                   <  4:299310/139‑1 (MQ=255)                                                                                                                            aaTTAATGGTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACGTGGTCAGAACCgg           >  7:205696/1‑147 (MQ=255)                                                                                                                                 aTGGTTGGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGGCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACGTGGTCAGAACCGGCAGAAcc    <  5:92150/149‑1 (MQ=255)                                                                                                                                    gTTTGAGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACGTGGTCAGAACCGGCAGAACCAt  <  6:101365/148‑1 (MQ=255)                                                                                                                                         |                                                                                                                                               CGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACGTGGTCAGAACCGGCAGAACCAT  >  NC_000913/446301‑446579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑