Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,869,858 A→G 5.1% C139C (TGT→TGC umpG ← broad specificity 5'(3')‑nucleotidase and polyphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,869,8580AG 5.1% 243.8 / 3.1 78C139C (TGT→TGCumpGbroad specificity 5'(3')‑nucleotidase and polyphosphatase
Reads supporting (aligned to +/- strand):  ref base A (33/41);  new base G (2/2);  total (35/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

GCGGGATCACCTGATCTGCCGGATGTCGTGTACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGATTCGGC  >  NC_000913/2869713‑2869993
                                                                                                                                                 |                                                                                                                                       
gcGGGATCACCTGATCTGCCGGATGTCGTGTACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGaa                                                                                                                                         >  4:215537/1‑146 (MQ=255)
gcGGGATCACCTGATCTGCCGGATGTCGTGTACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGaa                                                                                                                                         >  8:217519/1‑146 (MQ=255)
  gggATCACCTGATCTGCCGGATGTCGTGTACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAgg                                                                                                                                     <  1:387156/148‑1 (MQ=255)
    gATCACCTGATCTGCCGGATGTCGTGTACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGt                                                                                                                                    >  2:193892/1‑147 (MQ=255)
       cACCTGATCTGCCGGATGTCGTGTACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTAcc                                                                                                                                >  1:196986/1‑148 (MQ=255)
            gATCTGCCGGATGTCGTGTACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACcgccgc                                                                                                                           >  1:79042/1‑148 (MQ=255)
                 gCCGGATGTCGTGTACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAGCAGGTTACCGCCGCGGCAg                                                                                                                      >  7:56998/1‑148 (MQ=255)
                  ccGGATGTCGTGTACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGc                                                                                                                        <  1:193892/145‑1 (MQ=255)
                  ccGGATGTCGTGTACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCa                                                                                                                       <  6:118193/146‑1 (MQ=255)
                  ccGGATGTCGTGTACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCa                                                                                                                       <  8:218757/146‑1 (MQ=255)
                  ccGGATGTCGTGTACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCa                                                                                                                       <  7:217519/146‑1 (MQ=255)
                   cGGATGTCGTGTACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAATTCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAGCAGGTTACCGCCGCGGCAgtg                                                                                                                    >  6:11893/1‑148 (MQ=255)
                    ggATGTCGTGTACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCGCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAgtgt                                                                                                                   <  5:391802/148‑1 (MQ=255)
                      aTGTCGTGTACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCg                                                                                                                 >  3:245144/1‑148 (MQ=255)
                         tCGTGTACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTa                                                                                                               <  8:246178/147‑1 (MQ=255)
                         tCGTGTACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGAAGTGTCGTaa                                                                                                              >  2:54557/1‑148 (MQ=255)
                          cGTGTACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGACCGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACCGGTTACCGCCGCGGCAGTGTCGTAAt                                                                                                             >  7:97606/1‑148 (MQ=255)
                            tgtACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGt                                                                                                           <  8:102905/148‑1 (MQ=255)
                              tACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGTAGTGTCGTAATGttt                                                                                                         <  1:54557/148‑1 (MQ=255)
                              tACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGttt                                                                                                         <  2:69290/148‑1 (MQ=255)
                              tACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGttt                                                                                                         <  1:154982/148‑1 (MQ=255)
                              tACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGttt                                                                                                         <  3:378302/148‑1 (MQ=255)
                              tACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGttt                                                                                                         <  6:130881/148‑1 (MQ=255)
                              tACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGttt                                                                                                         <  2:79042/148‑1 (MQ=255)
                                ccGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTa                                                                                                        <  2:196986/147‑1 (MQ=255)
                                      cgcgTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGt                                                                                                 <  5:301541/148‑1 (MQ=255)
                                          tCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAg                                                                                             <  2:172821/148‑1 (MQ=255)
                                           cACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGttt                                                                                                         <  3:39609/135‑1 (MQ=255)
                                           cACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGttt                                                                                                         >  4:39609/1‑135 (MQ=255)
                                             cgcgAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGc                                                                                            >  2:363597/1‑146 (MQ=255)
                                              gcgAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCgag                                                                                         <  1:31654/148‑1 (MQ=255)
                                              gcgAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTCACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCgag                                                                                         <  8:175829/148‑1 (MQ=255)
                                                   tACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGc                                                                                    <  8:412497/148‑1 (MQ=255)
                                                        ttgatttgatCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAgcg                                                                               >  7:368606/1‑148 (MQ=255)
                                                        ttgatttgatCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAgcg                                                                               >  3:328060/1‑148 (MQ=255)
                                                        ttgatttgatCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAgcg                                                                               >  5:8326/1‑148 (MQ=255)
                                                           atttgatCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGcc                                                                             <  8:368606/147‑1 (MQ=255)
                                                           atttgatCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCg                                                                            <  8:97606/148‑1 (MQ=255)
                                                            tttgatCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCg                                                                            >  7:200962/1‑147 (MQ=255)
                                                             ttgatCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCgg                                                                           >  3:20417/1‑147 (MQ=255)
                                                                atCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAGCAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGaaa                                                                        <  8:56998/147‑1 (MQ=255)
                                                                 tCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAAc                                                                      >  5:150014/1‑148 (MQ=255)
                                                                  ccAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCTCAAAATTGAACAg                                                                                                                                      <  6:253617/83‑1 (MQ=255)
                                                                  ccAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAg                                                                                                                                      >  5:253617/1‑83 (MQ=255)
                                                                    aaGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTa                                                                   <  4:245144/148‑1 (MQ=255)
                                                                    aaGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTa                                                                   <  3:378049/148‑1 (MQ=255)
                                                                      gggTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCa                                                                                                                       >  3:234066/1‑94 (MQ=255)
                                                                      gggTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCa                                                                                                                       <  4:234066/94‑1 (MQ=255)
                                                                        gTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATg                                                               <  1:50021/148‑1 (MQ=255)
                                                                            aTCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTa                                                                                                               <  1:289313/96‑1 (MQ=255)
                                                                            aTCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTa                                                                                                               >  2:289313/1‑96 (MQ=255)
                                                                               cGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGcc                                                         <  1:363597/147‑1 (MQ=255)
                                                                                 gAACGTTAACATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTc                                                      <  3:66852/148‑1 (MQ=255)
                                                                                 gAACGTTAACATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTc                                                      >  4:66852/1‑148 (MQ=255)
                                                                                       tAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTCCATcgc                                                >  7:185830/1‑148 (MQ=255)
                                                                                          tatTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTCCATCgcggc                                             >  7:119350/1‑148 (MQ=255)
                                                                                            ttAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGttt                                                                                                         >  6:131401/1‑86 (MQ=255)
                                                                                            ttAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGttt                                                                                                         <  5:131401/86‑1 (MQ=255)
                                                                                            ttAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTCCATCGcggcgg                                           >  7:276488/1‑148 (MQ=255)
                                                                                                 aaTACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTCCATCGCGGCGGCtacc                                      >  7:199360/1‑148 (MQ=255)
                                                                                                  aTACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTCCATCGCGGCGGCtaccg                                     <  3:333943/148‑1 (MQ=255)
                                                                                                  aTACGCCCGGTGCGCAGCGGCGCGTTACACAGTGCGCGCCACATTGCACAGGTTACCGCCGCGGCAGTGGCGTAATGTTTATGCCCGTCACGCGAGCCGGCCAGCGCCGGAAACCCTAAAGGCCGGCCGTCCCTCGCGGCGGCtaccg                                     >  8:262581/1‑148 (MQ=255)
                                                                                                    aCGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTcc                                                     >  4:349007/1‑130 (MQ=255)
                                                                                                    aCGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTcc                                                     <  3:349007/130‑1 (MQ=255)
                                                                                                       cccGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTCCATCGCGGCGGCtaccgtaccg                                <  8:200962/148‑1 (MQ=255)
                                                                                                         ctgTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTCCATCGCGGCGGCTACCGTACCGGa                              <  5:39517/146‑1 (MQ=255)
                                                                                                                cAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTCCATCGCGGCGGCTACCGTACCGGaataaat                        >  1:350618/1‑147 (MQ=255)
                                                                                                                cAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTCCATCGCGGCGGCTACCGTACCGGaataaat                        >  1:330342/1‑147 (MQ=255)
                                                                                                                  gCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAAc                     <  2:350618/148‑1 (MQ=255)
                                                                                                                        ctTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACatcatc               >  7:347956/1‑148 (MQ=255)
                                                                                                                          ttACACTGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATccc             >  4:24504/1‑148 (MQ=255)
                                                                                                                          ttACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGc                                                                                                                        >  7:194077/1‑41 (MQ=255)
                                                                                                                          ttACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGc                                                                                                                        <  8:194077/41‑1 (MQ=255)
                                                                                                                           tACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATcccc            >  6:190695/1‑148 (MQ=255)
                                                                                                                                aGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGAt       >  1:390896/1‑148 (MQ=255)
                                                                                                                                  tGCGCGCAAAATTGAGCAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGATTc     <  5:11893/148‑1 (MQ=255)
                                                                                                                                  tGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGa                                                              >  6:248596/1‑91 (MQ=255)
                                                                                                                                  tGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGa                                                              <  5:248596/91‑1 (MQ=255)
                                                                                                                                     gcgcAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGATTCGGc  <  4:328060/148‑1 (MQ=255)
                                                                                                                                     gcgcAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGAGCCGGAAAACCTAAATGACGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGATTCGGc  <  3:215537/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                       
GCGGGATCACCTGATCTGCCGGATGTCGTGTACCGCAGCGCGTCACGCGAATACCTTTGATTTGATCCAAGGGTAAATCCGGAACGTTAATATTAAGAATACGCCCGGTGCGCAGCGGCTCTTTACACAGTGCGCGCAAAATTGAACAGGTTACCGCCGCGGCAGTGTCGTAATGTTTATGCCCGTCAAGCGAGACGGCAAGCGCCGGAAAACCTAAATGACGGCCTTCCATCGCGGCGGCTACCGTACCGGAATAAATAACATCATCCCCCAGATTCGGC  >  NC_000913/2869713‑2869993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: