Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,248,744 G→A 6.1% P292L (CCG→CTG)  yeiE ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,248,7440GA 6.1% 307.5 / 5.6 98P292L (CCG→CTG) yeiEputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (44/47);  new base A (3/3);  total (48/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01

Only 100 of 102 total aligned reads displayed.

CAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGAT  >  NC_000913/2248597‑2248888
                                                                                                                                                   |                                                                                                                                                
cAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAAcgcg                                                                                                                                                  >  1:4326/1‑148 (MQ=255)
    aaTACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGTca                                                                                                                                               >  5:95/1‑147 (MQ=255)
    aaTACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGca                                                                                                                                               >  4:265334/1‑147 (MQ=255)
      tACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACAt                                                                                                                                            >  3:48270/1‑148 (MQ=255)
      tACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACAt                                                                                                                                            <  2:4326/148‑1 (MQ=255)
       acacGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACAtt                                                                                                                                           >  4:158608/1‑148 (MQ=255)
         acGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTTGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTg                                                                                                                                         >  4:105007/1‑148 (MQ=255)
         acGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTg                                                                                                                                         <  6:95/148‑1 (MQ=255)
          cGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGc                                                                                                                                        <  5:52849/148‑1 (MQ=255)
                   gACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGc                                                                                                                                >  7:119133/1‑147 (MQ=255)
                    acacCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACCCAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCaa                                                                                                                              >  8:339110/1‑148 (MQ=255)
                     cacCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCTGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCTGGATCGCAAt                                                                                                                             <  4:334611/148‑1 (MQ=255)
                     cacCTTCATGATCGCCCAGGGAGTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCGGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAAt                                                                                                                             <  7:347140/148‑1 (MQ=255)
                       ccTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGTCGAATGCATGCTGACGGCGCTGAGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACCGCAACTTAACGCGGCACAATTTCGGGATCGCAATAg                                                                                                                           >  4:36150/1‑148 (MQ=255)
                        cTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCCTCACGAAATCGACACAGCACCAGCATGTTCTAGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGt                                                                                                                          >  7:298150/1‑148 (MQ=255)
                         ttCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTc                                                                                                                         >  5:146587/1‑148 (MQ=255)
                          tCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTc                                                                                                                         >  7:400799/1‑147 (MQ=255)
                          tCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGAATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTc                                                                                                                         >  5:203440/1‑147 (MQ=255)
                           cATGATCGCCCAGGGTTTATAAGTAAAGGAGCGAATCTCTTTTCTGGCGAATGCTTGTTGAAGGCGGTGCGTCAAGAAATCTACACAGCACCAGCATGTTGTTGTACAGTAACTTAACGAGGGACATTTGCGGGATCGCTATAGTcc                                                                                                                        <  6:203440/147‑1 (MQ=255)
                             tGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAg                                                                                                                      >  6:155107/1‑147 (MQ=255)
                                 cGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAgc                                                                                                                 >  2:243899/1‑148 (MQ=255)
                                     cAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCg                                                                                                             >  7:135523/1‑148 (MQ=255)
                                     cAGGGAGTATAAGTAAAGTAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCg                                                                                                             >  1:11098/1‑148 (MQ=255)
                                        ggATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATTTTCTTGTACAGCAACTTAACGCGGCTCATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAg                                                                                                          >  1:269105/1‑148 (MQ=255)
                                         gATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAgc                                                                                                         >  2:98503/1‑148 (MQ=255)
                                         gATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAgc                                                                                                         >  8:319272/1‑148 (MQ=255)
                                         gATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCAGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAgc                                                                                                         >  7:257549/1‑148 (MQ=255)
                                         gATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCAAGTTCTTGTACAGCAACTTAACGCAGCACATTTGCGGGATCGCAATAGTCGAGAAAGCGCCGTAgc                                                                                                         >  7:263803/1‑148 (MQ=255)
                                            tataAGTAAAGCAGCCAATCTCTTTTCGGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGcgcg                                                                                                      >  4:40689/1‑148 (MQ=255)
                                                 gTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTgg                                                                                                  >  5:388446/1‑147 (MQ=255)
                                                  tAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGGCACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGa                                                                                                 >  8:380834/1‑147 (MQ=255)
                                                  tAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGaa                                                                                                <  7:319272/148‑1 (MQ=255)
                                                   aaaGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGa                                                                                                 >  7:380483/1‑146 (MQ=255)
                                                    aaGTAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGaa                                                                                                <  2:11098/146‑1 (MQ=255)
                                                    aaGCAGCCTATGTCTTTTCTGGCGAATGCATGCTGATGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAAGTTAACGCAGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGaaa                                                                                               <  8:263803/147‑1 (MQ=255)
                                                    aaGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCAGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGaaa                                                                                               <  8:257549/147‑1 (MQ=255)
                                                         gcCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGtt                                                                                         <  3:36150/148‑1 (MQ=255)
                                                         gcCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGtt                                                                                         <  1:98503/148‑1 (MQ=255)
                                                         gcCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGtt                                                                                         <  8:119133/148‑1 (MQ=255)
                                                          ccAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGttt                                                                                        <  3:158608/148‑1 (MQ=255)
                                                             aTCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTg                                                                                     >  7:132453/1‑148 (MQ=255)
                                                             aTCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGACACGAAATAGACACAGCACCAGCATGTTAATTTACAGCAAATAAACGCTGCACATTTGAGCGATCCCAATAGTCCAGAAAGCGCCGTAGCGCGTAGGAAAGGTGTTTTTg                                                                                     >  4:148311/1‑148 (MQ=255)
                                                             aTCTCTTTTCTGGCGAATGCATGCGGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTg                                                                                     <  1:361227/148‑1 (MQ=255)
                                                              tctcTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGc                                                                                    >  4:191598/1‑148 (MQ=255)
                                                                  ttttCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGttt                                                                                        >  7:155170/1‑140 (MQ=255)
                                                                  ttttCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGttt                                                                                        <  8:155170/140‑1 (MQ=255)
                                                                  ttttCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGc                                                                                    <  5:284319/144‑1 (MQ=255)
                                                                  ttttCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCgat                                                                                >  6:161000/1‑148 (MQ=255)
                                                                    ttCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGCTCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCga                                                                                 <  5:285394/145‑1 (MQ=255)
                                                                    ttCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCgatga                                                                              >  5:232682/1‑148 (MQ=255)
                                                                         gCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCATCTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGtat                                                                         <  3:356846/148‑1 (MQ=255)
                                                                         gCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGatgatg                                                                            <  5:172009/145‑1 (MQ=255)
                                                                         gCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGatgatg                                                                            <  3:105007/145‑1 (MQ=255)
                                                                         gCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGtat                                                                         <  1:243899/148‑1 (MQ=255)
                                                                         gCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGtat                                                                         <  3:191598/148‑1 (MQ=255)
                                                                         gCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGtat                                                                         <  4:48270/148‑1 (MQ=255)
                                                                          cGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGGCACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGtata                                                                        <  7:380834/148‑1 (MQ=255)
                                                                                 atgcTGAAGGCGCTGCGTCACGAAATTGACACAGCACCAGCATGTTCTTGTACAGGAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGGGTTGGAAAGGTGTTTTTGCCGATGATGTATAGGCcaca                                                                 <  8:298150/148‑1 (MQ=255)
                                                                                   gcTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCAGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGa                                                                                                 <  6:201878/114‑1 (MQ=255)
                                                                                   gcTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCAGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGa                                                                                                 >  5:201878/1‑114 (MQ=255)
                                                                                      gAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTa                                                            >  4:324583/1‑148 (MQ=255)
                                                                                           cgcTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCAt                                                       <  3:40689/148‑1 (MQ=255)
                                                                                            gcTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATc                                                      <  4:322135/148‑1 (MQ=255)
                                                                                             cTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGt                                                                                                                          >  1:314754/1‑79 (MQ=255)
                                                                                             cTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGt                                                                                                                          <  2:314754/79‑1 (MQ=255)
                                                                                             cTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCAt                                                       >  1:195653/1‑146 (MQ=255)
                                                                                               gCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAgg                                                   >  7:153992/1‑148 (MQ=255)
                                                                                                  tCACGAAATCGACTCAGCACCAGGATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGATAGCGCCGTAGCGGGTTGGAATGGTGTTTTTg                                                                                     <  4:293856/111‑1 (MQ=255)
                                                                                                   cACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGcgcg                                                                                                      <  1:204386/93‑1 (MQ=255)
                                                                                                   cACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGcgcg                                                                                                      >  2:204386/1‑93 (MQ=255)
                                                                                                   cACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACaa                                                                <  5:294115/131‑1 (MQ=255)
                                                                                                   cACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACaa                                                                >  6:294115/1‑131 (MQ=255)
                                                                                                   cACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGcgcg                                               >  5:389053/1‑148 (MQ=255)
                                                                                                       aaaTCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCgg                                              >  1:59134/1‑145 (MQ=255)
                                                                                                         aTCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCagag                                         <  8:380483/148‑1 (MQ=255)
                                                                                                         aTCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGAGCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCagag                                         <  3:4621/148‑1 (MQ=255)
                                                                                                          tCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCgg                                                                                                                                                 >  1:291871/1‑43 (MQ=255)
                                                                                                          tCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCgg                                                                                                                                                 <  2:291871/43‑1 (MQ=255)
                                                                                                           cGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGtt                                                                                                    <  7:38181/87‑1 (MQ=255)
                                                                                                            gACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCCTTGGAAAGGTGATTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGa                                      >  1:91204/1‑148 (MQ=255)
                                                                                                             acacAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGAc                                     <  6:232682/148‑1 (MQ=255)
                                                                                                                 aGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCa                                 <  6:388446/148‑1 (MQ=255)
                                                                                                                     ccAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCagag                                         >  3:97557/1‑136 (MQ=255)
                                                                                                                     ccAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCagag                                         <  4:97557/136‑1 (MQ=255)
                                                                                                                      cAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTc                            <  5:155107/148‑1 (MQ=255)
                                                                                                                         cATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGt                                                                           <  7:281927/98‑1 (MQ=255)
                                                                                                                         cATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGt                                                                           >  8:281927/1‑98 (MQ=255)
                                                                                                                         cATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACt                         >  8:186755/1‑148 (MQ=255)
                                                                                                                          aTGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGCCCGCAACTTCACt                         >  4:276254/1‑147 (MQ=255)
                                                                                                                           tGTTCTTGTAGAGCAACTTAACGAGGTACATTTGCGTGATCGAAATAGTTCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAAAGTACGCATCAGGCGCGGCAGAGGGa                                      <  4:180096/133‑1 (MQ=255)
                                                                                                                            gTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCAAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGAc                                     >  3:180096/1‑133 (MQ=255)
                                                                                                                             ttCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTaa                      <  6:389053/147‑1 (MQ=255)
                                                                                                                                  gTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGtgc                 <  8:132453/147‑1 (MQ=255)
                                                                                                                                     cAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGtgcctg              <  8:135523/147‑1 (MQ=255)
                                                                                                                                     cAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGtgcctg              <  5:161000/147‑1 (MQ=255)
                                                                                                                                             tAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCa                                                        >  5:255622/1‑97 (MQ=255)
                                                                                                                                             tAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCa                                                        <  6:255622/97‑1 (MQ=255)
                                                                                                                                               aCGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTg    <  2:59134/147‑1 (MQ=255)
                                                                                                                                               aCGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGa   <  7:186755/148‑1 (MQ=255)
                                                                                                                                                cgcgGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGAt  >  1:65600/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGAT  >  NC_000913/2248597‑2248888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: