Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,100,923 A→G 100% S116P (TCA→CCA)  gnd ← 6‑phosphogluconate dehydrogenase, decarboxylating

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,100,9230AG96.8% 320.8 / 0.1 94S116P (TCA→CCA) gnd6‑phosphogluconate dehydrogenase, decarboxylating
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (45/44);  total (48/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAGCTACGGCGGCGATTTTGGTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGG  >  NC_000913/2100779‑2101059
                                                                                                                                                |                                                                                                                                        
cAGCTACGGCGGCGATTTTGGTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCTATGAAGTTAAAGCCCTCTGCTGgaa                                                                                                                                        <  1:163218/147‑1 (MQ=255)
cAGCTACGGCGGCGATTTTGGTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAg                                                                                                                                       >  4:374365/1‑148 (MQ=255)
  gCTACGGCGGCGATTTTGGTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCt                                                                                                                                     >  8:53195/1‑148 (MQ=255)
       ggcggcGATTTTGGTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGa                                                                                                                                >  3:67728/1‑148 (MQ=255)
         cggcgATTTTGGTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGAt                                                                                                                               >  6:204964/1‑147 (MQ=255)
             gATTTTGGTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTacga                                                                                                                          <  6:93851/148‑1 (MQ=255)
              aTTTTGGTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTacgac                                                                                                                         >  8:254870/1‑148 (MQ=255)
              aTTTTGCTCAGGATCGGTGCTACCAATTCATAGGCATCTTTCTGGCCACCAGGCATAATAGAACGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGCTACCGATGAAGTTAAAGCCCTCTGCTGGAACCTCACGATTacgac                                                                                                                         >  8:261093/1‑148 (MQ=255)
               ttttGGTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTacgac                                                                                                                         >  7:257739/1‑147 (MQ=255)
                  tGGTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGaa                                                                                                                      >  7:151086/1‑147 (MQ=255)
                    gTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAATACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATa                                                                                                                    <  7:121168/147‑1 (MQ=255)
                       aGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAgtgt                                                                                                                >  2:231952/1‑148 (MQ=255)
                                      aaTTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCCCGATTACGACGAATAGTGTCCTGGAAGAAGgtgt                                                                                                 >  5:136033/1‑148 (MQ=255)
                                      aaTTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGAt                                                                                                                               <  6:260544/118‑1 (MQ=255)
                                      aaTTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGAt                                                                                                                               >  5:260544/1‑118 (MQ=255)
                                      aaTTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGgtgt                                                                                                 >  8:168285/1‑148 (MQ=255)
                                        ttCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTa                                                                                               <  5:271952/148‑1 (MQ=255)
                                            tAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTaccac                                                                                           >  5:148275/1‑148 (MQ=255)
                                               gCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCGTTCAGCGCCCCCTCTTCACCGCCAGAAACACAGGTACCGATGAACTTAAAGACCACTCCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCAt                                                                                        >  5:106900/1‑148 (MQ=255)
                                                cttcttTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGGCCTGGAAGAAGGTGTTACCACCATc                                                                                       <  5:204964/148‑1 (MQ=255)
                                                          gCCACCATGCATAATAGAAGTACCTTTCCGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTTCTGTAAGCTAACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgatg                                                                                <  8:110974/145‑1 (MQ=255)
                                                          gCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgatgatg                                                                             <  2:155029/148‑1 (MQ=255)
                                                          gCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgatga                                                                               <  3:374365/146‑1 (MQ=255)
                                                          gCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgatg                                                                                <  8:151086/145‑1 (MQ=255)
                                                              ccaGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTa                                                                                                                             >  6:315785/1‑96 (MQ=255)
                                                              ccaGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTa                                                                                                                             <  5:315785/96‑1 (MQ=255)
                                                                aGGCATAATAGAAGGACCTTTCAGTGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCATGGAACAAGGTGTTACCACCATCAATCATGATGTATcca                                                                       >  8:145721/1‑147 (MQ=255)
                                                                   cATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgatg                                                                                >  3:163808/1‑136 (MQ=255)
                                                                   cATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgatg                                                                                <  4:163808/136‑1 (MQ=255)
                                                                   cATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTa                                                                    >  3:253185/1‑148 (MQ=255)
                                                                      aataGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATc                                                                  >  8:142805/1‑147 (MQ=255)
                                                                      aataGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATc                                                                  >  3:100266/1‑147 (MQ=255)
                                                                         aGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCg                                                                 <  4:257482/145‑1 (MQ=255)
                                                                         aGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCg                                                                 >  3:257482/1‑145 (MQ=255)
                                                                           aaGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATa                                                                                                                    >  7:55002/1‑92 (MQ=255)
                                                                           aaGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATa                                                                                                                    <  8:55002/92‑1 (MQ=255)
                                                                           aaGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTa                                                                    >  8:281128/1‑140 (MQ=255)
                                                                           aaGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTa                                                                    <  7:281128/140‑1 (MQ=255)
                                                                            aGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGatat                                                           <  4:100266/148‑1 (MQ=255)
                                                                               aCCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGt                                                        <  7:53195/148‑1 (MQ=255)
                                                                                ccTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAAGGATGATGTCTCCTTTATCGAGATATGGtt                                                       >  6:247727/1‑148 (MQ=255)
                                                                                 cTTTCAGTGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGttt                                                      <  7:145721/148‑1 (MQ=255)
                                                                                 cTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGttt                                                      >  3:257816/1‑148 (MQ=255)
                                                                                    tCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTg                                                     <  6:148152/146‑1 (MQ=255)
                                                                                    tCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTg                                                     <  7:142805/146‑1 (MQ=255)
                                                                                    tCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGa                                                    <  8:257739/147‑1 (MQ=255)
                                                                                     cAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAg                                                   <  7:168285/147‑1 (MQ=255)
                                                                                        cgcCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGaa                                               >  8:119474/1‑148 (MQ=255)
                                                                                         gcCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTATAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGGGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTTTCGAGATATGGTTTGAGGGAAt                                              <  7:261093/148‑1 (MQ=255)
                                                                                         gcCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAAt                                              <  7:254870/148‑1 (MQ=255)
                                                                                           ccccTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGGTGTCTCCTTTATCGAGATATGGTTTGAGGGAATCa                                            <  4:67728/148‑1 (MQ=255)
                                                                                             actctTCACAGCCATAAACAACTGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCaa                                           <  4:77392/146‑1 (MQ=255)
                                                                                              ctctTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGgtgt                                                                                                 <  8:128313/92‑1 (MQ=255)
                                                                                              ctctTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGgtgt                                                                                                 >  7:128313/1‑92 (MQ=255)
                                                                                              ctctTCACCGCCAGAAACACCGGTACCGAAGAAGATAAAGCCCAATGCTGCAAGCTCACGATTACCACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAAAGATGATGTCTACTTTAACGAGAAATTGTTTGAGGcaatcaat                                          >  3:77392/1‑147 (MQ=255)
                                                                                               tctTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGgaaga                                                                                                       >  2:116956/1‑85 (MQ=255)
                                                                                               tctTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGgaaga                                                                                                       <  1:116956/85‑1 (MQ=255)
                                                                                               tctTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGt                                                        <  6:372939/132‑1 (MQ=255)
                                                                                               tctTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGt                                                        >  5:372939/1‑132 (MQ=255)
                                                                                               tctTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATag                                        >  8:370416/1‑148 (MQ=255)
                                                                                                ctTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATag                                        >  8:415076/1‑147 (MQ=255)
                                                                                                ctTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATag                                        >  8:295566/1‑147 (MQ=255)
                                                                                                  tCACCGCCAGAAACACCGGTACCGATGAAGTTAAGGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgatga                                                                               >  7:281733/1‑106 (MQ=255)
                                                                                                  tCACCGCCAGAAACACCGGTACCGATGAAGTTAAGGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgatga                                                                               <  8:281733/106‑1 (MQ=255)
                                                                                                  tCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgat                                                                                 >  8:85081/1‑104 (MQ=255)
                                                                                                  tCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgat                                                                                 <  7:85081/104‑1 (MQ=255)
                                                                                                  tCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGttt                                                      >  7:399352/1‑131 (MQ=255)
                                                                                                  tCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGttt                                                      <  8:399352/131‑1 (MQ=255)
                                                                                                  tCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGttt                                                      <  8:393710/131‑1 (MQ=255)
                                                                                                  tCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGttt                                                      >  7:393710/1‑131 (MQ=255)
                                                                                                  tCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATagcag                                     >  5:43360/1‑148 (MQ=255)
                                                                                                   cACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATagc                                       <  7:415076/145‑1 (MQ=255)
                                                                                                        cgaGAAAACCCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCGCACGATTACTACGAATAGTGTCCTGGACGAAGGTGTTACCACCATCAATGATTATGTCTCCTTTATCGAGATATTGTTTGAGGGAATCAATAGCAGCATCCg                               <  5:118950/146‑1 (MQ=255)
                                                                                                        ccAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCg                               <  7:119474/148‑1 (MQ=255)
                                                                                                           gAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGc                            >  3:354277/1‑148 (MQ=255)
                                                                                                             aaCACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAAt                                              >  8:45889/1‑128 (MQ=255)
                                                                                                             aaCACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAAt                                              <  7:45889/128‑1 (MQ=255)
                                                                                                             aaCACCGGTACCGATGAACTTAAAGCCCGCTGCTGGAAGCTCACGACTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCACTGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCt                          <  4:151998/148‑1 (MQ=255)
                                                                                                              acacCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTg                         <  6:43360/148‑1 (MQ=255)
                                                                                                                      aCCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTcc                                                                                                              <  4:372196/55‑1 (MQ=255)
                                                                                                                      aCCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTcc                                                                                                              >  3:372196/1‑55 (MQ=255)
                                                                                                                         gATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATag                                        >  2:339125/1‑122 (MQ=255)
                                                                                                                         gATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATag                                        <  1:339125/122‑1 (MQ=255)
                                                                                                                           tGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCAc            >  3:214642/1‑148 (MQ=255)
                                                                                                                             aaGTTAAAGGCCTGTGCTTGAAGCTCACGATTACGTCGAATAGTGTCCTTGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCAcc           <  6:106900/147‑1 (MQ=255)
                                                                                                                                ttAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATc                                             <  8:296993/110‑1 (MQ=255)
                                                                                                                                ttAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATc                                             >  7:296993/1‑110 (MQ=255)
                                                                                                                                ttAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCa          >  5:257069/1‑145 (MQ=255)
                                                                                                                                ttAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCa          <  6:257069/145‑1 (MQ=255)
                                                                                                                                   aaGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTaa      >  6:93774/1‑146 (MQ=255)
                                                                                                                                     gCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAgg  <  4:253185/148‑1 (MQ=255)
                                                                                                                                       ccTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCa                       >  4:285777/1‑125 (MQ=255)
                                                                                                                                       ccTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCa                       <  3:285777/125‑1 (MQ=255)
                                                                                                                                            gctgGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTAt                                                                   <  4:370211/76‑1 (MQ=255)
                                                                                                                                            gctgGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTAt                                                                   >  3:370211/1‑76 (MQ=255)
                                                                                                                                                |                                                                                                                                        
CAGCTACGGCGGCGATTTTGGTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGG  >  NC_000913/2100779‑2101059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑