Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,939,871 G→A 5.3% P108L (CCG→CTG)  lpxM ← myristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,939,8710GA 5.3% 246.7 / 2.4 75P108L (CCG→CTG) lpxMmyristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (32/39);  new base A (2/2);  total (34/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

TTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTT  >  NC_000913/1939725‑1940017
                                                                                                                                                  |                                                                                                                                                  
ttttCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCgg                                                                                                                                                   <  8:126643/148‑1 (MQ=255)
 tttCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGATGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGc                                                                                                                                                  >  3:324351/1‑148 (MQ=255)
 tttCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGc                                                                                                                                                  >  7:45665/1‑148 (MQ=255)
       cccTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCcg                                                                                                                                               <  4:283454/145‑1 (MQ=255)
        ccTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTa                                                                                                                                           <  8:20629/148‑1 (MQ=255)
         cTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTAt                                                                                                                                          >  5:85780/1‑148 (MQ=255)
         cTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTAt                                                                                                                                          >  2:342173/1‑148 (MQ=255)
          ttGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTAtt                                                                                                                                         >  5:146908/1‑148 (MQ=255)
                ggCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAAc                                                                                                                                   <  2:64916/148‑1 (MQ=255)
                  ccATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTc                                                                                                                                 <  3:170366/148‑1 (MQ=255)
                  ccATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTc                                                                                                                                 <  8:201405/148‑1 (MQ=255)
                   cATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCcg                                                                                                                                               >  1:256728/1‑133 (MQ=255)
                   cATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCcg                                                                                                                                               <  2:256728/133‑1 (MQ=255)
                    aTCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAg                                                                                                                               >  7:286551/1‑148 (MQ=255)
                    aTCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAg                                                                                                                               <  3:80726/148‑1 (MQ=255)
                      cAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCAGCCCGCGTATTGCCAACTCAGc                                                                                                                              >  7:320566/1‑147 (MQ=255)
                      cAGCATGGCAAGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCAGCCCGCGTATTGCCAACTCAGc                                                                                                                              >  7:387243/1‑147 (MQ=255)
                       aGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGcc                                                                                                                             <  2:293079/147‑1 (MQ=255)
                       aGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCAGCCCGCGTATTGCCAACTCAGcc                                                                                                                             <  8:320566/147‑1 (MQ=255)
                         cATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCcatc                                                                                                                          <  4:180393/148‑1 (MQ=255)
                         cATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCcatc                                                                                                                          <  5:170040/148‑1 (MQ=255)
                         cATGGCAGGAATATCGACGGCCCAACCGGGCGGCACCAGAACGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCTGTCAACGCGCGGCTGACTTTTCTCCGGCCCGCGTATTGCCAACTCAGCcatc                                                                                                                          <  1:245659/148‑1 (MQ=255)
                         cATGGCAAGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCAGCCCGCGTATTGCCAACTCAGCcatc                                                                                                                          <  8:387243/148‑1 (MQ=255)
                          aTGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGGTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCcatca                                                                                                                         <  4:115234/148‑1 (MQ=255)
                                  aagatCGCCGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCCTc                                                                                                                 >  3:117760/4‑148 (MQ=255)
                                  aaTATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCACCGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATc                                                                                                                 >  3:117758/1‑148 (MQ=255)
                                  aaTATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATc                                                                                                                 <  8:45665/148‑1 (MQ=255)
                                     atCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCt                                                                                                              >  6:139042/1‑148 (MQ=255)
                                         aCGGCCCAACCGTGCTGCACCAGAAAGATAAATTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGAATTGCCAACTCAGCCATCATTGCCATCGCTTgcg                                                                                                          >  2:143875/1‑148 (MQ=255)
                                             cccAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGgcgc                                                                                                      <  7:350553/148‑1 (MQ=255)
                                              ccAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGcgcg                                                                                                     <  3:289641/148‑1 (MQ=255)
                                                  ccGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTgg                                                                                                 >  3:203267/1‑148 (MQ=255)
                                                      gCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCaaa                                                                                             >  5:188227/1‑148 (MQ=255)
                                                         gCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACAt                                                                                          <  8:92034/148‑1 (MQ=255)
                                                         gCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACAt                                                                                          <  6:146908/148‑1 (MQ=255)
                                                            ccAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACAtctc                                                                                       <  4:324351/148‑1 (MQ=255)
                                                             cAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGt                                                                                                                                            <  7:108147/94‑1 (MQ=255)
                                                             cAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGt                                                                                                                                            >  8:108147/1‑94 (MQ=255)
                                                             cAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCtca                                                                                      <  8:286551/148‑1 (MQ=255)
                                                             cAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCtca                                                                                      <  6:188227/148‑1 (MQ=255)
                                                              aGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTAt                                                                                                                                          >  1:55926/1‑95 (MQ=255)
                                                              aGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTAt                                                                                                                                          <  2:55926/95‑1 (MQ=255)
                                                              aGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCtcat                                                                                     <  1:337076/148‑1 (MQ=255)
                                                               gAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGAAGCTCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATATTCTCCGGCCCGCCTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCTCTGTGGCAAACATCtcat                                                                                     >  8:390563/1‑147 (MQ=255)
                                                                    aTAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGAGGCGCGGTGGCAAACATCTCATcaacaa                                                                               >  5:298602/1‑148 (MQ=255)
                                                                       aCTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGcc                                                                                                                             <  8:384118/99‑1 (MQ=255)
                                                                       aCTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGcc                                                                                                                             >  7:384118/1‑99 (MQ=255)
                                                                         tttCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCt                                                                          >  5:157286/1‑148 (MQ=255)
                                                                          ttCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCtt                                                                         >  8:222438/1‑148 (MQ=255)
                                                                          ttCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCtt                                                                         >  3:267076/1‑148 (MQ=255)
                                                                          ttCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCtt                                                                         >  5:319680/1‑148 (MQ=255)
                                                                           tctcGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCtt                                                                         <  1:342173/147‑1 (MQ=255)
                                                                               gTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGt                                                                    <  6:157286/148‑1 (MQ=255)
                                                                                       gccgcATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTc                                                            <  4:203267/148‑1 (MQ=255)
                                                                                        ccgcATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCa                                                           <  5:139042/148‑1 (MQ=255)
                                                                                                 ttCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGtt                                                    <  6:278798/146‑1 (MQ=255)
                                                                                                  tCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTg                                                 >  5:256762/1‑148 (MQ=255)
                                                                                                  tCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTg                                                 >  2:190745/1‑148 (MQ=255)
                                                                                                     atgatCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGa                                               >  4:173122/1‑147 (MQ=255)
                                                                                                        atCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCaaa                                                                                             <  8:44291/98‑1 (MQ=255)
                                                                                                        atCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAAATTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCaaa                                                                                             >  7:44291/1‑98 (MQ=255)
                                                                                                              aGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCtcat                                                                                     >  4:121183/1‑100 (MQ=255)
                                                                                                              aGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCtcat                                                                                     <  3:121183/100‑1 (MQ=255)
                                                                                                                        aGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAgattgat                           <  3:173122/148‑1 (MQ=255)
                                                                                                                          tCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCaga                                        >  8:203304/1‑133 (MQ=255)
                                                                                                                          tCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCaga                                        <  7:203304/133‑1 (MQ=255)
                                                                                                                          tCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAgattgatt                          >  4:232430/1‑147 (MQ=255)
                                                                                                                           cAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTaa                        >  6:285799/1‑148 (MQ=255)
                                                                                                                             aCGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTc                                                            <  8:118798/110‑1 (MQ=255)
                                                                                                                             aCGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTc                                                            >  7:118798/1‑110 (MQ=255)
                                                                                                                              cgcgcgGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGc                     <  4:267076/148‑1 (MQ=255)
                                                                                                                                     cTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACgg              <  3:232430/148‑1 (MQ=255)
                                                                                                                                       gAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACCTCTCATCAACAATCGCTTCGCGGTCAGCTTCCCTACGTGCTGGAAAGCAGAGCGACAGATTGATTACCGCCCGACggag            >  2:263076/1‑146 (MQ=255)
                                                                                                                                          ttttCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGa         <  2:142105/148‑1 (MQ=255)
                                                                                                                                          ttttCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGa         <  6:319680/148‑1 (MQ=255)
                                                                                                                                                 cGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCttttt  <  6:298602/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCATCAACAATCGCTTCGCGTTCAGCTTCACTACGTTCTGGAAAGCAGAGCGACAGATTGATTAACGCACGACGGCGTGAGCTTTTT  >  NC_000913/1939725‑1940017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑