Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,548,986 C→T 5.9% P529L (CCG→CTG)  fdnG → formate dehydrogenase‑N, alpha subunit, nitrate‑inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,548,9860CT 5.9% 214.2 / 2.3 67P529L (CCG→CTG) fdnGformate dehydrogenase‑N, alpha subunit, nitrate‑inducible
Reads supporting (aligned to +/- strand):  ref base C (26/37);  new base T (2/2);  total (28/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTATCTGGAAGCGAACACGCCGAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCTTTAACCCGGTTGCGTCCTTCCCGGACAAAAACAAAGTGGTGAGCTGCCTGAGCAAGCTGAAGTA  >  NC_000913/1548839‑1549133
                                                                                                                                                   |                                                                                                                                                   
cGTATCTGGAAGCGAACACGCCGAAAGCGACGATGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTATTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACCACTGGGCCTATTACTTGCTGcc                                                                                                                                                     >  6:296767/1‑148 (MQ=255)
        gAAGCGAACACGCCGAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTggg                                                                                                                                             <  6:233937/148‑1 (MQ=255)
        gAAGCGAACACGCCGAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTggg                                                                                                                                             <  2:73554/148‑1 (MQ=255)
        gAAGCGAACACGCCGAAAGCGACGCAGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAg                                                                                                                                                 <  6:134376/144‑1 (MQ=255)
         aaGCGAACACGCCGAAAGCGCCGCTGGCTGATCAGGGGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGa                                                                                                                                            <  3:277116/148‑1 (MQ=255)
         aaGCGAACACGCCGAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGa                                                                                                                                            <  4:161632/148‑1 (MQ=255)
         aaGCGAACACGCCGAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGa                                                                                                                                            <  3:182138/148‑1 (MQ=255)
            cGAACACGCCGAAAGCGACGCTGGCTGATCTGGTGAACTTCTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGATATCTTTCTATGGCGATGCCGCGCAGATAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGTAcc                                                                                                                                          <  1:274407/147‑1 (MQ=255)
                 acGCCGAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCt                                                                                                                                    <  2:22415/148‑1 (MQ=255)
                  cGCCGAAAGCGACGCTGGCGGAGCAGGTGAACTACTGGAGCAACTATCCGAAGTGCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTa                                                                                                                                   <  2:268294/148‑1 (MQ=255)
                      gAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCtactac                                                                                                                               >  5:209888/1‑148 (MQ=255)
                       aaaGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTacgacg                                                                                                                              >  6:258192/1‑148 (MQ=255)
                       aaaGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTAAGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTacgacg                                                                                                                              >  7:14052/1‑148 (MQ=255)
                        aaGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGt                                                                                                                             <  8:120845/148‑1 (MQ=255)
                          gCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGtca                                                                                                                           >  8:96302/1‑148 (MQ=255)
                           cGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGtcat                                                                                                                          <  6:116744/148‑1 (MQ=255)
                              cGCTGGCTGATCAGGTGAAATACTGGAGCAACTATCCGCAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTTGCTGCCGAAGTGGGACCAGACCTACGACGTCATCaa                                                                                                                       <  5:296767/148‑1 (MQ=255)
                                    ctgATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTAttt                                                                                                                 >  3:84360/1‑148 (MQ=255)
                                      gATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGTCTGCCGAAGTGGGACCAGAACTACGACGTCATCAAGTATTTc                                                                                                                >  5:97049/1‑147 (MQ=255)
                                       aTCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCATGATTAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCaa                                                                                                              <  4:72269/148‑1 (MQ=255)
                                           ggTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACatg                                                                                                          <  5:258192/148‑1 (MQ=255)
                                            gTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACatga                                                                                                         >  6:124332/1‑148 (MQ=255)
                                                 ctactGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATgatggat                                                                                                    >  8:327818/1‑148 (MQ=255)
                                                  tactGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATgatggat                                                                                                    >  7:45349/1‑147 (MQ=255)
                                                  tactGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATgatggat                                                                                                    >  7:63714/1‑147 (MQ=255)
                                                   actGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATgatggatg                                                                                                   >  8:326182/1‑147 (MQ=255)
                                                    ctGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGaa                                                                                                 <  5:137644/148‑1 (MQ=255)
                                                    ctGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGa                                                                                                  <  3:117270/147‑1 (MQ=255)
                                                        aGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGGGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCa                                                                                             <  5:58403/148‑1 (MQ=255)
                                                        aGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCTGAAGTGGGACCAGACCTACGACGtcatca                                                                                                                        <  6:297872/121‑1 (MQ=255)
                                                        aGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCTGAAGTGGGACCAGACCTACGACGtcatca                                                                                                                        >  5:297872/1‑121 (MQ=255)
                                                                    aaGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGGAGGCAAAGTCACCGGt                                                                                  >  4:141379/1‑147 (MQ=255)
                                                                            cggtAGCCTGATGAAATCTTTCTATGGCGATGCCGCGGAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGGCATCAAGTATTTCAACATGATGGATGAAGTCAAAGTCACCGGTTATTTc                                                                            <  2:120821/142‑1 (MQ=255)
                                                                            cGTTAGCCTGATGAAATCTTTCTACGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTc                                                                            <  8:14052/145‑1 (MQ=255)
                                                                             gTTTGCCTTATGTAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGcc                                                                        <  6:209888/148‑1 (MQ=255)
                                                                              ttAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGc                                                                         >  8:76584/1‑146 (MQ=255)
                                                                              ttAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGCCa                                                                       <  5:124332/148‑1 (MQ=255)
                                                                                   cTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTAttt                                                                                                                 <  8:108688/101‑1 (MQ=255)
                                                                                   cTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTAttt                                                                                                                 >  7:108688/1‑101 (MQ=255)
                                                                                     gatgaAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATgatggatg                                                                                                   <  4:219814/113‑1 (MQ=255)
                                                                                     gatgaAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATgatggatg                                                                                                   >  3:219814/1‑113 (MQ=255)
                                                                                       tgaAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGa                                                                                                  >  4:338419/1‑112 (MQ=255)
                                                                                       tgaAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGa                                                                                                  <  3:338419/112‑1 (MQ=255)
                                                                                       tgaAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGAGGGATGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCGTTaa                                                              <  3:68332/148‑1 (MQ=255)
                                                                                        gaAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCTGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCTTTAAc                                                             >  6:165071/1‑148 (MQ=255)
                                                                                               ttCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCa                                                                                       <  8:357053/115‑1 (MQ=255)
                                                                                               ttCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCa                                                                                       >  7:357053/1‑115 (MQ=255)
                                                                                               ttCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCTTTAACCCGGTTg                                                      >  1:198766/1‑148 (MQ=255)
                                                                                                tCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGtcatca                                                                                                                        <  3:360542/81‑1 (MQ=255)
                                                                                                tCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGtcatca                                                                                                                        >  4:360542/1‑81 (MQ=255)
                                                                                                        gATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCTTTAACCCGGTTGCGTCCTTcc                                             <  7:327818/148‑1 (MQ=255)
                                                                                                               cgcAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCTTTAACCCGGTTGCGTCCTTCCCGGACaa                                      <  7:395817/148‑1 (MQ=255)
                                                                                                                gcAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCTTTAACCCGGTTGCGTCCTTCCCGGACaa                                      >  7:234006/1‑147 (MQ=255)
                                                                                                                     aaGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAgg                                                                                               <  2:46664/85‑1 (MQ=255)
                                                                                                                     aaGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAgg                                                                                               >  1:46664/1‑85 (MQ=255)
                                                                                                                     aaGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCTTTAACCCGGTTGCGTCCTTCCCGGACAAAAACaa                                <  8:45349/148‑1 (MQ=255)
                                                                                                                      agagCACAACTGGGGCTAAGACTGGCTGCCGAACTGGGACCAGACCTAAGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAACTCACCGGGTATTTCTGCCACGGCTTTAACCCGGTTGCGACCTTCCCGGACAAAAACa                                 >  2:315997/1‑146 (MQ=255)
                                                                                                                       gagaACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCTTTAACCCGGTTGCGTCCTTCCCGGACAAAAACAAAg                              <  8:63714/148‑1 (MQ=255)
                                                                                                                         gaACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCTTTAACCCGGTTGCGTCCTTCCCGGACAAAAACAAAgtg                            <  7:326182/148‑1 (MQ=255)
                                                                                                                           acaacTGGGTCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCTTTAACCCGGTTGCGTCCTTCCCGGACAAAAACAAAgtggt                          <  1:312345/148‑1 (MQ=255)
                                                                                                                             aacTGGGGCTATGACTGGGTGCCGAAGTGGGACCAGACCTACGACGTCATCTAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCTTTAACCCGGTTGCGTCCTTCCCGGACAAAAACAAAGTGGTGa                        <  1:262872/148‑1 (MQ=255)
                                                                                                                                   ggCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGAGGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCTTTAACCCGGTTGCGTCCTTACCGGACAAAAACGAAGTGGTGAGCTGcc                  >  2:298301/1‑148 (MQ=255)
                                                                                                                                     cTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGGAGGCAAAGTCACCGGTTATTTCTGCCAGGGCTTTAACCCGGTTGCGTCCTTCCCGGACAAAAACAAAGTGGTGAGCTGCCTg                <  3:141379/148‑1 (MQ=255)
                                                                                                                                          aCTGGCTGCTGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCTTTAACCCGGTTGCGTCCTTCCCGGACAAAAACAAAGTGGTGAGCTGCCTGAGCaa           <  5:165071/148‑1 (MQ=255)
                                                                                                                                            tGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCTTTAACCCGGTTGCGTCCTTCCCGGACAAAAACAAAGTGGTGAGCTGCCTGAGCAAGc         >  5:207842/1‑148 (MQ=255)
                                                                                                                                              gCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCt                                                                  >  7:11376/1‑89 (MQ=255)
                                                                                                                                              gCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCt                                                                  <  8:11376/89‑1 (MQ=255)
                                                                                                                                                 gCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATAGATGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCTTTAACCCGGTTGCGTCCTTCCCGGACAAAAACAAAGTGGTGAGCTGCCTGAGCAAGCTGAAg    >  7:326325/1‑148 (MQ=255)
                                                                                                                                                  ccGAAGTGGGACCAGCCCTACGACGTCATCTAGTATTTGAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGCCCGGGCTTTAACCCGGTTGCGTCCTTCCCGGACAAAAACAAAGTGGTGAGCTGCCTGAGCAGGCTGAAGt   >  4:197658/1‑148 (MQ=255)
                                                                                                                                                  ccGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATAGATGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCTTTAACCCGGTTGCGTCCTTCCCGGACAAAAACAAAGTGGTGAGCTGCCTGAGCAAGCTGAAg    <  8:326325/147‑1 (MQ=255)
                                                                                                                                                   cGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCTTTAACCCGGTTGCGTCCTTCCCGGACAAAAACAAAGTGGTGAGCTGCCTGAGCAAGCTGAAGTa  >  5:131403/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGTATCTGGAAGCGAACACGCCGAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCTTTAACCCGGTTGCGTCCTTCCCGGACAAAAACAAAGTGGTGAGCTGCCTGAGCAAGCTGAAGTA  >  NC_000913/1548839‑1549133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: