Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,493,798 G→A 5.8% V443V (GTG→GTA trg → methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,493,7980GA 5.8% 219.2 / 2.9 69V443V (GTG→GTAtrgmethyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
Reads supporting (aligned to +/- strand):  ref base G (25/39);  new base A (2/2);  total (28/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

CGCGAGCGGGTGAGCAAGGGCGTGGATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTAGAGGCATAACGCAGGTTAGCCAGGCGATTTCTGAAATGGATAAGGTGAC  >  NC_000913/1493653‑1493944
                                                                                                                                                 |                                                                                                                                                  
cgcgAGCGGGTGAGCAAGGGCGTGGATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTgg                                                                                                                                                   >  5:338662/1‑147 (MQ=255)
cgcgAGCGGGTGAGCAAGGGCGTGGATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTgg                                                                                                                                                   >  5:95589/1‑147 (MQ=255)
cgcgAGCGGGTGAGCAAGGGCGTGGATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTgg                                                                                                                                                   >  6:204261/1‑147 (MQ=255)
        ggTGAGCAAGGGCGTGGATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCg                                                                                                                                          >  4:232925/1‑148 (MQ=255)
          tGAGCAAGGGCGTGGCTTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGCAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCggg                                                                                                                                        <  1:304073/148‑1 (MQ=255)
            aGCAAGGGCGTGGATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGa                                                                                                                                       <  2:99820/147‑1 (MQ=255)
             gCAAGGGCGTGCATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAATGAGATTGAACGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGaa                                                                                                                                      >  1:167676/1‑147 (MQ=255)
               aaGGGCGTGGATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAAc                                                                                                                                   <  2:199768/148‑1 (MQ=255)
                aGGGCGTGGATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCAGGAAAAcc                                                                                                                                  <  6:95589/148‑1 (MQ=255)
                        gATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCa                                                                                                                           <  5:204261/147‑1 (MQ=255)
                        gATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCAc                                                                                                                          >  6:364865/1‑148 (MQ=255)
                         aTTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACt                                                                                                                         <  8:125260/148‑1 (MQ=255)
                            tGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAt                                                                                                                       <  6:338662/147‑1 (MQ=255)
                              ccGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAttgt                                                                                                                    <  5:364865/148‑1 (MQ=255)
                                gTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAttgttg                                                                                                                  <  7:150852/148‑1 (MQ=255)
                                  tGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGAt                                                                                                                >  7:372533/1‑148 (MQ=255)
                                       ccAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGt                                                                                                           >  8:10359/1‑148 (MQ=255)
                                           cGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTcgcg                                                                                                       <  2:81934/148‑1 (MQ=255)
                                           cGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTcgc                                                                                                        >  8:331712/1‑147 (MQ=255)
                                           cGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTcgc                                                                                                        >  8:5326/1‑147 (MQ=255)
                                            gAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAATGCTTGATCAGTGAATCAGTCAGGTTAATTGCCCGGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTcga                                                                                                        >  1:306261/1‑145 (MQ=255)
                                                 aCGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAttgttg                                                                                                                  <  5:63001/131‑1 (MQ=255)
                                                 aCGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAttgttg                                                                                                                  >  6:63001/1‑131 (MQ=255)
                                                  cGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTc                                                                                                 <  5:249467/147‑1 (MQ=255)
                                                    gACACTGGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGTAGGCTTGATCAGGGAATCAGTCCGGTTAATTGACCGGGGGGCGGATGAGGGGGCAAGGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTcaca                                                                                              <  2:167676/148‑1 (MQ=255)
                                                    gACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGTTGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGa                                                                                                                 <  2:372330/129‑1 (MQ=255)
                                                    gACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGa                                                                                                                 >  1:372330/1‑129 (MQ=255)
                                                          cGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGATGGCTTGATCAGTGAATCAGTCAGGTTAATTGAACTCGGGTAGGATGAGGTTGCAACGGCAGGGAAAACCATGAGAACTATTGTTGATGCCGTCGCGAGTGTCACACatat                                                                                         >  8:190071/1‑147 (MQ=255)
                                                          cGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAttgttg                                                                                                                  >  7:205592/1‑122 (MQ=255)
                                                          cGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAttgttg                                                                                                                  <  8:205592/122‑1 (MQ=255)
                                                           gCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATc                                                                                        <  1:190850/147‑1 (MQ=255)
                                                                   cAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGaa                                                                               <  8:372533/148‑1 (MQ=255)
                                                                     gcgcTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAAt                                                                             <  7:33121/148‑1 (MQ=255)
                                                                            ggcggcGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGc                                                                                    <  1:195075/134‑1 (MQ=255)
                                                                            ggcggcGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGc                                                                                    >  2:195075/1‑134 (MQ=255)
                                                                                   aaaGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCg                                                                >  8:351135/1‑147 (MQ=255)
                                                                                    aaGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCACGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCggg                                                              <  3:242295/148‑2 (MQ=255)
                                                                                    aaGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTAGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGa                                                              >  4:113428/1‑148 (MQ=255)
                                                                                    aaGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTAGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGa                                                              >  4:118554/1‑148 (MQ=255)
                                                                                    aaGAGATTGAAGGCGTGATCAGTGAATCAGTCATGTTAATTGACCAGGGGTCGGAGTAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGGCGCGAGGGTCACACATATCATGCAGGAAATCGCCGCCGCCTCgg                                                               <  6:151526/147‑1 (MQ=255)
                                                                                     agagATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGTGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCa                                                                                   <  5:374622/126‑1 (MQ=255)
                                                                                     agagATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGTGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCa                                                                                   >  6:374622/1‑126 (MQ=255)
                                                                                             aaGGCGTGATCCTTTAATAAGTCATTTTAATAGACCTGTGTTCTGATGATGTGTCAACGGCCGGTAAAACCATGAGCACTATTTTTTATGCCGTCGCGCGTGGCACACATATCCTGCAGGAAATCGCCTACGCCTCGTATGAACaac                                                      <  2:306261/147‑2 (MQ=255)
                                                                                              aGGCTTGATCAGTGAATCAGTCAGGTTAATTGCCCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGCGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAg                                                     >  6:294956/1‑147 (MQ=255)
                                                                                                gCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATc                                                                                        >  4:371594/1‑110 (MQ=255)
                                                                                                gCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATc                                                                                        <  3:371594/110‑1 (MQ=255)
                                                                                                gCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTag                                                  <  7:5326/148‑1 (MQ=255)
                                                                                                   tGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAgtgt                                                                                                  <  3:196856/97‑1 (MQ=255)
                                                                                                   tGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAgtgt                                                                                                  >  4:196856/1‑97 (MQ=255)
                                                                                                    gATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTagag                                                <  7:331712/146‑1 (MQ=255)
                                                                                                     aTCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCAAGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTAGAGGCa                                             <  3:232925/148‑1 (MQ=255)
                                                                                                       cAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTAGAGGCAt                                            >  7:187104/1‑147 (MQ=255)
                                                                                                           gAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACATAGTAGAGGCATAACg                                        >  3:116258/1‑147 (MQ=255)
                                                                                                            aaTCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTAGAGGCATAACGc                                       <  1:7981/147‑1 (MQ=255)
                                                                                                            aaTCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTAGAGGCATAACGCa                                      <  7:10359/148‑1 (MQ=255)
                                                                                                                    agGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGGCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTAGAGGCATAACGCAGGTTAGcc                              <  1:223491/148‑1 (MQ=255)
                                                                                                                             gACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTAGAGGCATAACGCAg                                     <  2:183042/132‑1 (MQ=255)
                                                                                                                             gACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTAGAGGCATAACGCAg                                     >  1:183042/1‑132 (MQ=255)
                                                                                                                                   ggggCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTAGAGGCATAACGCAGGTTAGCCAGGCGATTTCTGaa                <  6:171255/147‑1 (MQ=255)
                                                                                                                                   gggTCGGATGAGGTAGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTAGAGGCATAACGCAGGTTAGCCAGGCGATTTCTGaa                <  3:118554/147‑1 (MQ=255)
                                                                                                                                   gggTCGGATGAGGTAGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTAGAGGCATAACGCAGGTTAGCCAGGCGATTTCTGaa                <  3:113428/147‑1 (MQ=255)
                                                                                                                                    ggTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTAGAGGCATAACGCAGGTTAGCCAGGCGATTTCTGAAAt              <  1:16400/148‑1 (MQ=255)
                                                                                                                                     gTCGGATGAGGTGGCAACTGCCGTTAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTAGAGGCATAACGCAGGTTAGCCAGGCGATTTCTGAAATg             <  7:190071/148‑1 (MQ=255)
                                                                                                                                       cGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATcg                                                                           <  1:295898/84‑1 (MQ=255)
                                                                                                                                       cGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATcg                                                                           >  2:295898/1‑84 (MQ=255)
                                                                                                                                             aGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTAGAGGCATAACGCAgg                                    >  1:156727/1‑117 (MQ=255)
                                                                                                                                             aGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTAGAGGCATAACGCAgg                                    <  2:156727/117‑1 (MQ=255)
                                                                                                                                             aGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTAGAGGCATAACGCAGATTAGCCAGGCGATTTCTGAAATGGATAAgg      <  7:351135/147‑1 (MQ=255)
                                                                                                                                              ggTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTAGAGGCATAACGCAGGTTAGCCAGGCGATTTCTGAAATGGATAAGGTg    >  8:124662/1‑148 (MQ=255)
                                                                                                                                              ggTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGACGCGAGTGTCACACATATCATGCAGGAAATCGCTGCCGCCTCGGATGAACAAAGTAGAGGCATAACGCAGGTTAGCCAGGCGATTTCTGAAATGGATAAGGt     >  6:315009/1‑147 (MQ=255)
                                                                                                                                                tGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTAGAGGCATAACGCAGGTTAGCCAGGCGATTTCTGAAATGGATAAGGTGAc  >  3:286703/1‑148 (MQ=255)
                                                                                                                                                tGGCAAAGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTTGAGGCATAACGCAGGt                                   <  3:261257/115‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CGCGAGCGGGTGAGCAAGGGCGTGGATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAATCGCCGCCGCCTCGGATGAACAAAGTAGAGGCATAACGCAGGTTAGCCAGGCGATTTCTGAAATGGATAAGGTGAC  >  NC_000913/1493653‑1493944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: