Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 530,694 | G→A | 6.3% | A178V (GCG→GTG) | mnmH ← | tRNA 2‑selenouridine synthase, selenophosphate‑dependent |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 530,694 | 0 | G | A | 6.3% | 205.3 / 2.6 | 64 | A178V (GCG→GTG) | mnmH | tRNA 2‑selenouridine synthase, selenophosphate‑dependent |
Reads supporting (aligned to +/- strand): ref base G (27/32); new base A (2/2); total (30/34) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01 |
GGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTC > NC_000913/530551‑530837 | ggTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGa < 8:25084/148‑1 (MQ=255) cGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGa > 8:31721/1‑148 (MQ=255) aaCCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATg < 7:77047/148‑1 (MQ=255) aCCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGa < 8:198528/148‑1 (MQ=255) cTTTCGTCTTCCAGCACCCACAGGCGCAACTTCTGACGGGCGTCGGTTTTTCGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCtt < 6:368268/148‑1 (MQ=255) tCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTcc < 4:27909/147‑1 (MQ=255) tCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTc < 7:208703/146‑1 (MQ=255) tCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTc < 5:81277/146‑1 (MQ=255) ggcTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGGCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGa < 4:3652/146‑1 (MQ=255) gTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAAGCGCGATGACGCGCCAACCCTTCCAGa < 4:120050/148‑1 (MQ=255) tCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGAt < 3:180078/148‑1 (MQ=255) cTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAAc > 8:194575/1‑137 (MQ=255) cTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAAc < 7:194575/137‑1 (MQ=255) tCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCaa > 5:112774/1‑148 (MQ=255) aCCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGt < 6:112774/148‑1 (MQ=255) ccACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGcc > 3:366935/1‑124 (MQ=255) ccACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGcc < 4:366935/124‑1 (MQ=255) cacaGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTcc < 3:134170/132‑1 (MQ=255) cacaGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTcc > 4:134170/1‑132 (MQ=255) aGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCt > 4:373955/1‑148 (MQ=255) aGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCt > 8:270681/1‑148 (MQ=255) cgcAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGtt < 8:247973/148‑1 (MQ=255) cgcgCGTCGGTTTTTAGCATTTCGGCAGCAAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTTGTTGTAACGTGCGACCAAACGCAGAACCGCGATGACGAGCAAACCCAGCCACATCAACACCGTTCGGCTGTTGCTGCACTaa > 8:387644/4‑146 (MQ=255) tCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTtgctgc < 1:170504/135‑1 (MQ=255) tCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTtgctgc > 2:170504/1‑135 (MQ=255) ggTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGtttt < 7:31721/147‑1 (MQ=255) ttCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACcgcg < 2:328498/75‑1 (MQ=255) ttCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAAc < 1:125863/88‑1 (MQ=255) ttCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAAc > 2:125863/1‑88 (MQ=255) ttCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAaca < 1:213590/103‑1 (MQ=255) ttCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAaca < 1:207502/103‑1 (MQ=255) ttCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAaca > 2:207502/1‑103 (MQ=255) ttCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAaca > 2:213590/1‑103 (MQ=255) ttCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTtgctgc < 1:269063/121‑1 (MQ=255) ttCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTtgctgc > 2:269063/1‑121 (MQ=255) ttCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAAAGCCGAACcgcg > 1:328498/1‑75 (MQ=255) agcagGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCt > 5:392468/1‑81 (MQ=255) agcagGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCt < 6:392468/81‑1 (MQ=255) cagGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGt > 8:125695/1‑95 (MQ=255) cagGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGt < 7:125695/95‑1 (MQ=255) cagGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTtgc < 8:114715/106‑1 (MQ=255) cagGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTtgc > 7:114715/1‑106 (MQ=255) ggTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAg > 5:86555/1‑148 (MQ=255) ggTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAg > 5:387397/1‑148 (MQ=255) cAAAACTCGCCTGGCGAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCGTCCAGATCAACACCGTTCGGCTGTTGCTCCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTAt > 6:176242/1‑148 (MQ=255) aaaaCTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAATCGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTAt < 1:391855/147‑1 (MQ=255) aaaaCTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTAt < 1:254648/147‑1 (MQ=255) ggCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGc < 8:313273/131‑1 (MQ=255) ggCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGc > 7:313273/1‑131 (MQ=255) ggCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGc > 5:294887/1‑148 (MQ=255) gCTAAGTTGTGTTTGTAACGTGCGCCCAAACTCCGAACCGCGATGACGCGCCAACCCTTCAATATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACACCCTCCAATAAGCACTTTAGGTTTTtgttc > 4:36536/1‑145 (MQ=255) gCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGcc < 6:86555/148‑1 (MQ=255) cTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCg > 5:355258/1‑97 (MQ=255) cTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCg < 6:355258/97‑1 (MQ=255) tgGTTGTAACGTGCGACCAAACACCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCaa > 4:254018/1‑146 (MQ=255) tgGTTGTAACGTGCGACCAAACACCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATa > 8:385495/1‑148 (MQ=255) gTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAg < 3:373955/147‑1 (MQ=255) gTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCg < 6:387397/147‑1 (MQ=255) aCGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCt > 2:29611/1‑147 (MQ=255) cGTGCGACCAAACACCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGa < 7:385495/148‑1 (MQ=255) cGTGCGACCAAACACCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGa < 3:254018/148‑1 (MQ=255) gCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTCCCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTGTTTGTGCCAGTTCAATAGTCGCCTGAATc > 8:175528/1‑148 (MQ=255) aCCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATcgcg > 4:5852/1‑148 (MQ=255) aaaCGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTc > 4:62705/1‑148 (MQ=255) | GGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTTGTGGTTGTAACGTGCGACCAAACGCCGAACCGCGATGACGCGCCAACCCTTCCAGATCAACACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTC > NC_000913/530551‑530837 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |