Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	510,362	C→T	5.9%	E340K (GAA→AAA)	copA ←	copper transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	510,362	0	C	T	5.9%	221.9 / 3.0	68	E340K (GAA→AAA)	copA	copper transporter
Reads supporting (aligned to +/- strand):  ref base C (32/32);  new base T (2/2);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GGACGCTATCGCCTTCGCCTTTTTGCTGCGGGATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGATCAGACCGATAATCA  >  NC_000913/510215‑510508                                                                                                                                                    |                                                                                                                                                   ggACGCTATCGCCTTCGCCTTTTTGCTGCGGGATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTc                                                                                                                                                    >  3:50969/1‑148 (MQ=255) ggACGCTATCGCCTTCGCCTTTTTGCTGCGGGATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTc                                                                                                                                                    >  6:184212/1‑148 (MQ=255)     gCTATCGCCTTCGCCTTTTTGCTGCGGGATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGcc                                                                                                                                                <  6:309267/148‑1 (MQ=255)     gCTATCGCCTTCGCCTTTATGCGGCGGGAGTGGTTCGCCCGTCAGCATCGCTTCCTCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATCCCTGGCTGCACTTCTGcc                                                                                                                                                <  3:41358/148‑1 (MQ=255)               tcgccttTTTGCTGCGGGATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGc                                                                                                                                      >  1:7786/1‑148 (MQ=255)                cgccttTTTGCTGCGGGATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCt                                                                                                                                     >  1:178102/1‑148 (MQ=255)                   cttTTTGCTGCGGGATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCtttt                                                                                                                                  <  6:214396/148‑1 (MQ=255)                               ggATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGt                                                                                                                       >  6:283026/1‑147 (MQ=255)                               ggATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTc                                                                                                                      <  6:167569/148‑1 (MQ=255)                                 aTTGGTTCGCCCGTCCGCATCGCTTCATCCAGCCATGCTTCGCCCTCGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCTTCAg                                                                                                                    >  4:337353/1‑148 (MQ=255)                                   tGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGt                                                                                                                   >  1:101131/1‑147 (MQ=255)                                   tGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGt                                                                                                                   >  5:81632/1‑147 (MQ=255)                                            cGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGt                                                                                                         <  2:178102/148‑1 (MQ=255)                                            cGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGt                                                                                                         >  6:58120/1‑148 (MQ=255)                                             gTCAGCATCGCTTCCTCCAGCCATGCTTCGCCCTGGGTCATCTCGCCATCTATCGGCACGCGCTCGCCGGGCGTCAGGCGCAGCAACATACCTGGCGGCACTGCTGCCAGAGGCACGCTTGTTTCACCTTCGTCAGTAACCAGGCGTg                                                                                                        <  2:257186/148‑1 (MQ=255)                                                     cGCTTCATCCAGCCATGCTCCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTcggc                                                                                                >  1:395251/1‑148 (MQ=255)                                                       cTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGTCGTcggcgg                                                                                              >  8:178658/1‑148 (MQ=255)                                                         tCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCgggg                                                                                            >  3:47997/1‑148 (MQ=255)                                                          cATCCAGCCATGCTTCGCCCTTGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCggg                                                                                             <  2:101131/146‑1 (MQ=255)                                                              cAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTTCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTaaa                                                                                       <  6:81632/148‑1 (MQ=255)                                                              cAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACg                                                                                                                                       >  2:16971/1‑100 (MQ=255)                                                              cAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACg                                                                                                                                       <  1:16971/100‑1 (MQ=255)                                                               aGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAAt                                                                                      <  8:168818/148‑1 (MQ=255)                                                               aGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAAt                                                                                      >  8:241458/1‑148 (MQ=255)                                                               aGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAAt                                                                                      <  8:262636/148‑1 (MQ=255)                                                                 ccATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCg                                                                                    <  4:47997/148‑1 (MQ=255)                                                                      cTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTa                                                                                <  4:50969/147‑1 (MQ=255)                                                                            ccTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTc                                                                         <  5:283026/148‑1 (MQ=255)                                                                             cTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGTCGTCGGCGGGGTTAAATCGAGTAACTTTTcc                                                                        <  7:178658/148‑1 (MQ=255)                                                                              tGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTc                                                                         >  6:143556/1‑146 (MQ=255)                                                                                      ctcGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTc                                                                         >  5:185903/1‑138 (MQ=255)                                                                                      ctcGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTc                                                                         <  6:185903/138‑1 (MQ=255)                                                                                      ctcGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCt                                                                 >  5:247572/1‑146 (MQ=255)                                                                                      ctcGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTATCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAAATTTTCCAGCGCCt                                                                 >  5:86303/1‑146 (MQ=255)                                                                                        cGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTag                                                              >  5:84792/1‑147 (MQ=255)                                                                                        cGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTag                                                              >  2:73291/1‑147 (MQ=255)                                                                                           cATCTACCGGCACGCGATCGCCGGTCGTCAGGAGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTagaaga                                                          <  1:73291/148‑1 (MQ=255)                                                                                                aCCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCt                                                     >  8:73792/1‑148 (MQ=255)                                                                                                aCCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCt                                                     >  6:383572/1‑148 (MQ=255)                                                                                                 ccGGCCAGCGATCGCCGGTCGTCAGGCGCAGCAAGATAACTGGCTGCACTTCAGCCAGCGGCACGCTTGTGTCAGCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGGTAATTCGAGGAACTTTTCCAGCGCCTTAGAAGAACGCTg                                                    >  2:311601/1‑148 (MQ=255)                                                                                                 ccGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAAc                                                        <  6:247572/144‑1 (MQ=255)                                                                                                  cGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTgg                                                   >  4:255644/1‑148 (MQ=255)                                                                                                  cGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTgg                                                   <  7:393056/148‑1 (MQ=255)                                                                                                       cgcgATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTgc                                              >  8:228204/1‑148 (MQ=255)                                                                                                        gcgATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTgg                                                   >  1:237146/1‑142 (MQ=255)                                                                                                        gcgATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTgg                                                   <  2:237146/142‑1 (MQ=255)                                                                                                               ccGGTCGGCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCCGGCGTGCCGTCGGCGGGGTTACATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCAGGCGTGCGCGCGCtt                                      <  1:311601/148‑1 (MQ=255)                                                                                                                cGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTc                                     <  6:378627/148‑1 (MQ=255)                                                                                                                  gtcgtcAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTACCCAGGCGTGCCGTCGGAGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCa                                   >  2:220652/1‑148 (MQ=255)                                                                                                                  gtcgtcAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTc                                     >  2:164343/1‑146 (MQ=255)                                                                                                                         ggCGCAGCAACATACCTGGCTGCACTTTTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAg                                  <  8:238614/142‑1 (MQ=255)                                                                                                                         ggCGCAGCAACATACCTGGCTGCACTTTTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAg                                  <  8:232626/142‑1 (MQ=255)                                                                                                                         ggCGCAGCAACATACCTGGCTGCACTTTTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAg                                  >  7:238614/1‑142 (MQ=255)                                                                                                                         ggCGCAGCAACATACCTGGCTGCACTTTTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAg                                  >  7:232626/1‑142 (MQ=255)                                                                                                                         ggCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCa                                                                                                              <  5:2713/66‑1 (MQ=255)                                                                                                                         ggCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCa                                                                                                              >  6:2713/1‑66 (MQ=255)                                                                                                                         ggCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATg                            <  4:114315/148‑1 (MQ=255)                                                                                                                              agcaACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCg                        <  1:358685/147‑1 (MQ=255)                                                                                                                               gcaACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGcc                         >  2:358685/1‑145 (MQ=255)                                                                                                                               gcaACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCgag                      <  1:164343/148‑1 (MQ=255)                                                                                                                                   cATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTg                  <  3:255644/148‑1 (MQ=255)                                                                                                                                    aTACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGTCCGAGATTGa                 <  5:184212/148‑1 (MQ=255)                                                                                                                                    aTACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGa                 <  5:143556/148‑1 (MQ=255)                                                                                                                                     tACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGAt                >  2:397968/1‑148 (MQ=255)                                                                                                                                     tACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGAt                <  5:58120/148‑1 (MQ=255)                                                                                                                                         tGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCat                               >  3:160780/1‑129 (MQ=255)                                                                                                                                         tGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCat                               >  3:166473/1‑129 (MQ=255)                                                                                                                                         tGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCat                               <  4:160780/129‑1 (MQ=255)                                                                                                                                         tGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCat                               <  4:166473/129‑1 (MQ=255)                                                                                                                                                aCTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGATCAGACCGATaa     >  3:49472/1‑148 (MQ=255)                                                                                                                                                    cTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGATCAGACCGATAatca  <  2:395251/147‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GGACGCTATCGCCTTCGCCTTTTTGCTGCGGGATTGGTTCGCCCGTCAGCATCGCTTCATCCAGCCATGCTTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGATCAGACCGATAATCA  >  NC_000913/510215‑510508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑