Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,906,168	IS5 (+) +4 bp	coding (397‑400/837 nt)	bglG ←	transcriptional antiterminator of the bgl operon

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2288919 =	NA (NA)	52 (1.350)	38/256	0.2	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	3906168 =	0 (0.000)					coding (400/837 nt)	bglG	transcriptional antiterminator of the bgl operon
*	?	NC_000913	= 3653230	NA (NA)	51 (1.300)	48/260	0.0	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	= 3906171	0 (0.000)					coding (397/837 nt)	bglG	transcriptional antiterminator of the bgl operon

ACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3653087‑3653230 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAATATGGAGGATGTTGCAGGTGTCACGCAGTT  <  NC_000913/3906171‑3906032                                                                                                                                                                                                                                                                                              ACAAGAAAATCTCTTTTCTGGTCTGACGGCGCGTACTGCTGAATTCACTGTCGGCGAAGGGGAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGG                                                                                                                                         <  6:130767/149‑1     GAAAATCTCTTATCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATTAACCCTGTTCTAAGGCTCCAGATGACAAACATGATCTCATATCAGGGACTAGTTCGCACCTACCCTAGGGGAA                                                                                                                                     >  5:185252/1‑149            TCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGT                                                                                                                              >  4:35839/1‑149            TCTTTTATGGTCTGACGGCGGTTTGTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCAAGATGACAAACATGATCTCTTATCAGGGACTTGTTCGCAGCTTCCCTAGGGGAAGAAGCGT                                                                                                                              <  6:185252/149‑1                     GTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTA                                                                                                                     >  4:156661/1‑149                      TCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACC                                                                                                                         <  6:112207/144‑1                      TCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACC                                                                                                                         >  5:112207/1‑144                             GCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAA                                                                                                             >  1:151730/1‑149                              CGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAAC                                                                                                            <  1:25900/149‑1                               GCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACG                                                                                                           >  4:59783/1‑149                                     TGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGC                                                                                                                                <  3:60983/122‑1                                     TGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGC                                                                                                                                >  4:60983/1‑122                                         GAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTG                                                                                                 >  2:210654/1‑149                                           ATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCA                                                                                               <  3:59783/149‑1                                                CTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTAC                                                                                          <  3:35839/149‑1                                                   TCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGGTGGGCGGGCAGTTACCGA                                                                                       <  3:156661/149‑1                                                            GTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAG                                                                              >  4:79818/1‑149                                                            GTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAG                                                                              <  1:210654/149‑1                                                             TAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGT                                                                             >  8:48810/1‑149                                                               AGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTG                                                                            <  4:12146/148‑1                                                                  TGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCT                                                                        >  1:35175/1‑149                                                                            TGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCAT                                                              <  2:35175/149‑1                                                                            TGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCG                                                                                        <  8:90909/123‑1                                                                            TGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCG                                                                                        >  7:90909/1‑123                                                                            TGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTAC                                                                                          <  3:56656/121‑1                                                                            TGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTAC                                                                                          >  4:56656/1‑121                                                                                GAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCAT                                                          >  8:22598/1‑149                                                                                   CCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTG                                                       >  2:60408/1‑149                                                                                     CTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGT                                                     <  2:151730/149‑1                                                                                           TATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGG                                                                          >  2:48616/1‑122                                                                                           TATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGG                                                                          <  1:48616/122‑1                                                                                           TATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAG                                                                                                                                 >  2:83681/1‑67                                                                                           TATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAG                                                                                                                                 <  1:83681/67‑1                                                                                             TGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTG                                                <  3:79818/146‑1                                                                                                        GACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGG                                   >  4:119254/1‑148                                                                                                          CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAA                                <  7:22598/149‑1                                                                                                          CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAA                                >  7:160966/1‑149                                                                                                               ATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATACATCTGGTCAGTGCCAAAATGAGCGGAAATATG                            >  3:91828/1‑148                                                                                                                TGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAATATG                            >  6:39156/1‑147                                                                                                                 GATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAATATGGAG                         >  1:73259/1‑149                                                                                                                       ATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAATATGGAGGATG                     <  2:73259/147‑1                                                                                                                        TATCAGGGACTTGTTCTCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGTAAATATGGAGGATGTTG                  <  7:48810/149‑1                                                                                                                          TCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAATATGGAGGATGTTGC                 <  5:39156/148‑1                                                                                                                           CAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGT                                                 >  5:167710/1‑115                                                                                                                           CAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGT                                                 <  6:167710/115‑1                                                                                                                            AGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAATATGGAGGATGTTGCAGG              >  2:202515/1‑149                                                                                                                            AGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATACATCTGGTCAGTGCCAAAATGAGCGGAAATATGGAGGATGTTGCAG               <  4:91828/148‑1                                                                                                                                  TTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCCTGCATCTGGTCAGTGCCCAAATGAGCGGAAATATGGAGGATGTTGCAGGTGTCAC        >  8:87415/1‑149                                                                                                                                     TTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAATATGGAGGATGTTGCAGGTGTCACG       >  1:98952/1‑147                                                                                                                                            CTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAATATGGAGGATGTTGCAGGTGTCACGCAGTT  >  4:89814/1‑145                                                                                                                                            CTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAATATGGAGGATGTTGCAGGTGTCACGCAGTT  <  3:89814/145‑1                                                                                                                                                                                                                                                                                              ACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3653087‑3653230 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAATATGGAGGATGTTGCAGGTGTCACGCAGTT  <  NC_000913/3906171‑3906032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.