Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,418,506 C→T 5.7% R476C (CGT→TGT)  yfcC → putative inner membrane transporter; C4‑dicarboxylate anaerobic carrier family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,418,5060CT 5.7% 221.6 / 2.4 71R476C (CGT→TGT) yfcCputative inner membrane transporter; C4‑dicarboxylate anaerobic carrier family protein
Reads supporting (aligned to +/- strand):  ref base C (33/33);  new base T (2/2);  total (35/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

GCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCT  >  NC_000913/2418358‑2418653
                                                                                                                                                    |                                                                                                                                                   
gCCGTTACTGGCACCGCTTGGCGATCTGGACGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTCGTGATCGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATCGCAACGCTCGCTGTGTGCAGGCTGGACTTcc                                                                                                                                                     >  1:237717/1‑149 (MQ=255)
 ccGTTACTGGCACCTCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTcc                                                                                                                                                     <  6:85580/148‑1 (MQ=255)
 ccGGTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGGCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCg                                                                                                                                                    <  1:181157/149‑1 (MQ=255)
      aCTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAAc                                                                                                                                                <  2:234191/148‑1 (MQ=255)
      aCTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAAc                                                                                                                                                <  7:225481/148‑1 (MQ=255)
          gCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCt                                                                                                                                           >  8:37497/1‑149 (MQ=255)
           cACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCt                                                                                                                                           >  6:184233/1‑148 (MQ=255)
                cTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCTTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCTGTACTGGCTGAAGGTg                                                                                                                                    >  8:237305/1‑149 (MQ=255)
                cTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCTGTACTGGCTGAAGGTg                                                                                                                                    >  8:243046/1‑149 (MQ=255)
                 ttGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTg                                                                                                                                    >  1:108254/1‑149 (MQ=255)
                            gTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGAccc                                                                                                                          >  7:355358/1‑148 (MQ=255)
                            gTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGAccc                                                                                                                          <  2:171015/148‑1 (MQ=255)
                                   ttAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGa                                                                                                                  <  3:164379/149‑1 (MQ=255)
                                       ccGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGCCCCTACTTGGActgc                                                                                                              >  6:170537/1‑149 (MQ=255)
                                        cGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGActgct                                                                                                             <  8:351695/149‑1 (MQ=255)
                                          tCAGGTTACCGTGCTGGTGTTACAGTTTGGTGATGGCTTCAGTCACATAATTTACACAACCTCAGCTTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGCCTTCCGTAACTGGCTGAAGGTGCGCGCGACCCTACTTGGActgcatt                                                                                                           >  6:197084/1‑146 (MQ=255)
                                              gTTTCCGTGCTGGCGTTCGAGTTTGGTGATGGCTTCAGTCACTTCATTTAGCCAACCTCTGCGTCGTTAATGGAAACTCTCGTTGTGTGCAGGGTGGACTTCCGTAACTGGCTGATGGTGGGCGCGACGCTACTTGGTCTGGTGTttat                                                                                                       <  6:253479/149‑1 (MQ=255)
                                              gTTACCGTGCTGGCGTTCCAGTTTGGTGCTGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGGGTGTGCAGGGGGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTttat                                                                                                       <  7:306014/149‑1 (MQ=255)
                                                 aCCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTttatta                                                                                                     <  7:37497/148‑1 (MQ=255)
                                                 aCCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTttatta                                                                                                     <  6:15270/148‑1 (MQ=255)
                                                 aCCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTtattat                                                                                                    >  1:78874/1‑149 (MQ=255)
                                                          gCGTTCCAGTGTGGTGCTGGCCTCACTCACATCATTTACCCAACCTCCGCGGCGTTACTGGCACCGCTCGGTGTGGGCAGGGTGGACTTCCGTAACTGGCTGCAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGcg                                                                                            <  6:52518/148‑1 (MQ=255)
                                                            gTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGcgtcg                                                                                         >  7:287811/1‑149 (MQ=255)
                                                            gTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGcgtcg                                                                                         >  5:44343/1‑149 (MQ=255)
                                                               ccAGTTTGGTGATGGATTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTa                                                                                       <  6:185978/148‑1 (MQ=255)
                                                                cAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGt                                                                                     >  1:63107/1‑149 (MQ=255)
                                                                      ggTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCgg                                                                               >  1:175886/1‑149 (MQ=255)
                                                                      ggTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCg                                                                                <  5:184233/148‑1 (MQ=255)
                                                                       gTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTGTTATGTCCAGCGTCGTAGTGATCGgc                                                                              >  4:314938/1‑149 (MQ=255)
                                                                         gATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGgcgc                                                                            >  1:239707/1‑149 (MQ=255)
                                                                           tGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTc                                                                          <  2:239707/149‑1 (MQ=255)
                                                                             gCTTCAGTCTCATCATTTACCCAACCTCAGCGTAGTTAATGTCAACGCTCGGTGGGGTCAGTGTGGACTTCCGTAACTGGCTGAATGTGGTAGCGACCCTACTTGGAATGCTGTTTATTATGTCGAGCGTCGTAGTGTTCGGCGCTCa                                                                         <  2:237717/148‑1 (MQ=255)
                                                                                 cAGTGACATCATTTACCCAACCTCAGCGGCGTTTATGGCAACGCTCTGTGTGTGCAGGGTTGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGtt                                                                      <  5:197084/147‑1 (MQ=255)
                                                                                  aGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGAt                                                                   <  8:355358/149‑1 (MQ=255)
                                                                                  aGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTGATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGAt                                                                   >  6:284554/1‑149 (MQ=255)
                                                                                    tCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATgg                                                                 >  4:121613/1‑149 (MQ=255)
                                                                                       catcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCt                                                              <  8:287811/149‑1 (MQ=255)
                                                                                        atcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCTGTACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTAc                                                            <  7:237305/149‑1 (MQ=255)
                                                                                        atcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCTGTACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTAc                                                            <  7:243046/149‑1 (MQ=255)
                                                                                        atcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTa                                                             >  6:327928/1‑149 (MQ=255)
                                                                                         tcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTGTTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTAc                                                            <  3:314938/149‑1 (MQ=255)
                                                                                         tcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTAc                                                            >  5:141554/1‑149 (MQ=255)
                                                                                         tcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTAc                                                            <  2:78874/149‑1 (MQ=255)
                                                                                         tcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTAc                                                            >  4:234375/1‑149 (MQ=255)
                                                                                         tcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTAc                                                            >  5:240244/1‑149 (MQ=255)
                                                                                         tcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGCGTGTGCAGGGGGGACTGCAGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCAAGCGTCGTAGTGATCGGCGCTCAGTTTATGGGCTAc                                                            <  6:338450/149‑1 (MQ=255)
                                                                                               aCCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTa                                                       >  6:71266/1‑148 (MQ=255)
                                                                                               aCCCAACCTCAGCGTCGTTAATGGCAACGCGCGGGGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCa                                                                         <  2:192688/130‑1 (MQ=255)
                                                                                                cccAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTaaa                                                     <  6:141554/149‑1 (MQ=255)
                                                                                                cccAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTaa                                                      >  2:176765/1‑148 (MQ=255)
                                                                                                cccAACCTCAGAGTCGTTAATGGCAACGCTCCGTGTGTGCAGGGTGGCCTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTAc                                                                                                                       >  1:242578/1‑83 (MQ=255)
                                                                                                     ccTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGt                                                <  7:152898/149‑1 (MQ=255)
                                                                                                       tCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGtt                                               >  2:90825/1‑148 (MQ=255)
                                                                                                        cAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTaa                                             <  5:71266/149‑1 (MQ=255)
                                                                                                                ttAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGa                                                                                                                  <  7:70283/72‑1 (MQ=255)
                                                                                                                ttAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGa                                                                                                                  >  8:70283/1‑72 (MQ=255)
                                                                                                                   aTGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAAtt                                  >  4:109285/1‑149 (MQ=255)
                                                                                                                      gCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGc                                <  7:125071/148‑1 (MQ=255)
                                                                                                                      gCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCg                               >  2:114237/1‑149 (MQ=255)
                                                                                                                      gCAACGCTCAGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGc                                <  5:45080/148‑1 (MQ=255)
                                                                                                                        aaCGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGc                             >  7:35927/1‑149 (MQ=255)
                                                                                                                           gCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTttatt                                                                                                      >  2:25984/1‑73 (MQ=255)
                                                                                                                           gCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTttatt                                                                                                      <  1:25984/73‑1 (MQ=255)
                                                                                                                             tCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGCCTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCttt                        >  5:232987/1‑149 (MQ=255)
                                                                                                                             tCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTa                                                                                       <  6:132756/86‑1 (MQ=255)
                                                                                                                             tCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTa                                                                                       >  5:132756/1‑86 (MQ=255)
                                                                                                                               ggTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCttttt                      >  2:91758/1‑149 (MQ=255)
                                                                                                                               ggTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCttttt                      <  3:234375/149‑1 (MQ=255)
                                                                                                                                 ggtgtgCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCGGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCtttt                       <  5:284554/145‑1 (MQ=255)
                                                                                                                                     tgCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCAtt                 <  1:114237/148‑1 (MQ=255)
                                                                                                                                      gCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCt               >  2:203625/1‑149 (MQ=255)
                                                                                                                                            tGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGtt         >  2:60812/1‑149 (MQ=255)
                                                                                                                                               aCTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCa      <  1:90825/149‑1 (MQ=255)
                                                                                                                                                   ccGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGtct  >  7:48161/1‑149 (MQ=255)
                                                                                                                                                   ccGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGtc   <  6:240244/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCT  >  NC_000913/2418358‑2418653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: