Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,934,587	T→C	62.9%	L6L (TTA→TTG)	edd ←	6‑phosphogluconate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,934,587	0	T	C	62.9%	66.4 / 65.4	70	L6L (TTA→TTG)	edd	6‑phosphogluconate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (7/19);  new base C (18/26);  total (25/45)
Fisher's exact test for biased strand distribution p-value = 3.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGCAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAA  >  NC_000913/1934445‑1934732                                                                                                                                               |                                                                                                                                                  gctggcAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAAc                                                                                                                                                <  4:147033/146‑1 (MQ=255) gctggcAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAAc                                                                                                                                                <  6:193003/146‑1 (MQ=255)     gcaggcagCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATtgt                                                                                                                                          <  7:118697/148‑1 (MQ=255)     gcaggcagCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATtgt                                                                                                                                          <  7:125672/148‑1 (MQ=255)       aggcagCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGa                                                                                                                                       >  1:364826/1‑149 (MQ=255)           agCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCa                                                                                                                                   <  2:109562/149‑1 (MQ=255)              gAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATaa                                                                                                                                <  4:188548/149‑1 (MQ=255)              gAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATa                                                                                                                                 >  4:25488/1‑148 (MQ=255)                aaCCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAg                                                                                                                              >  6:143454/1‑149 (MQ=255)                    gtgtGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTc                                                                                                                          <  1:270467/149‑1 (MQ=255)                             gTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATCCATAAAGGCTCCTGAAAtt                                                                                                                  >  6:378207/1‑148 (MQ=255)                                aCCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAg                                                                                                               <  5:276753/148‑1 (MQ=255)                                aCCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAg                                                                                                               <  2:152798/148‑1 (MQ=255)                                aCCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAg                                                                                                               <  5:331583/148‑1 (MQ=255)                                   gCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGt                                                                                                           >  8:16752/1‑149 (MQ=255)                                     aTGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCa                                                                                                         <  5:49990/149‑1 (MQ=255)                                     aTGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCa                                                                                                         <  4:291382/149‑1 (MQ=255)                                     aTGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAgg                                                                                                                             >  5:107959/1‑129 (MQ=255)                                     aTGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAgg                                                                                                                             <  6:107959/129‑1 (MQ=255)                                        ccAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTTTCCGGGCGAGATAAGCAGAGCGAGTCTCTCGCGAACGTTCTATGTTTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCaga                                                                                                       <  1:195392/148‑1 (MQ=255)                                         cAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTc                                                                                                                                    >  8:156136/1‑118 (MQ=255)                                          aaCTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGGGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCa                                                                                                    <  2:372299/149‑1 (MQ=255)                                               cGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGgatg                                                                                               <  1:400162/149‑1 (MQ=255)                                                gAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGgatga                                                                                              >  7:314683/1‑149 (MQ=255)                                                gAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGgatga                                                                                              <  3:25488/149‑1 (MQ=255)                                                    gATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTca                                                                                          >  7:308304/1‑149 (MQ=255)                                                    gATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTca                                                                                          >  2:163126/1‑149 (MQ=255)                                                        aaCGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATGCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACaa                                                                                       <  5:378207/148‑1 (MQ=255)                                                            gTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGAt                                                                                                                                      >  8:341048/1‑97 (MQ=255)                                                            gTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGAt                                                                                                                                      <  7:341048/97‑1 (MQ=255)                                                               gAAGTTTTCGCTTGTTCTTTCCGGTCGAGATAAGCAGAGCGAGTCTCGATCGAACGTTCAATGATTCGATTTGTTACGCGTAAAAATTGTGGATTCATAAAGTCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttc                                                                               <  1:368383/149‑1 (MQ=255)                                                               gAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGttt                                                                                >  2:135075/1‑148 (MQ=255)                                                                 aGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACCCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttcat                                                                             <  3:220952/149‑1 (MQ=255)                                                                     ttCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttcattca                                                                          <  7:146725/148‑1 (MQ=255)                                                                       cGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCaga                                                                        <  8:339550/148‑1 (MQ=255)                                                                        gCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttcattca                                                                          <  4:94799/145‑1 (MQ=255)                                                                        gCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttcattca                                                                          >  3:94799/1‑145 (MQ=255)                                                                         cTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGa                                                                     <  6:161492/149‑1 (MQ=255)                                                                            gTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGAtt                                                                   >  6:183641/1‑148 (MQ=255)                                                                             ttCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGAttt                                                                  <  8:314683/148‑1 (MQ=255)                                                                             ttCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGAttt                                                                  <  7:16752/148‑1 (MQ=255)                                                                                  tCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACt                                                            <  2:51250/149‑1 (MQ=255)                                                                                  tCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACt                                                            <  5:143454/149‑1 (MQ=255)                                                                                  tCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGa                                                              <  8:134016/147‑1 (MQ=255)                                                                                  tCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACt                                                            >  8:391076/1‑149 (MQ=255)                                                                                    cGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGa                                                          <  4:327594/149‑1 (MQ=255)                                                                                           gatattcAGAGGGAGTCTCGCGCGAAAGTTCAATGATTCGATTTTTTACGCTTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACTCGTTTCATTCAGAGGATTTATGACTTAAACGCCt                                                   <  4:334516/143‑1 (MQ=255)                                                                                           gaTAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCt                                                   <  7:391076/149‑1 (MQ=255)                                                                                            aTAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTg                                                  >  3:54125/1‑149 (MQ=255)                                                                                             tAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCTATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTg                                                  <  8:115471/148‑1 (MQ=255)                                                                                               aGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTaa                                               <  5:183641/149‑1 (MQ=255)                                                                                                  agagCGAGTCTCGCGCGCACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCg                                            >  1:134299/1‑149 (MQ=255)                                                                                                            tcGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAg                                  >  5:272484/1‑149 (MQ=255)                                                                                                              gcgcgAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATa                                   <  1:163126/146‑1 (MQ=255)                                                                                                              gcgcgAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAggg                                >  3:120833/1‑149 (MQ=255)                                                                                                               cgcgAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGc                               <  5:139620/149‑1 (MQ=255)                                                                                                                          aaTGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACTCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGttt                     >  1:312065/1‑148 (MQ=255)                                                                                                                          aaTGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTAAGAGCAGGATGATTCATAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGttt                     >  6:214360/1‑148 (MQ=255)                                                                                                                           aTGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGttt                     >  2:306190/1‑147 (MQ=255)                                                                                                                              aTTCGCTTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTTAAACGCCTGTAACCGGAGCTGATAGGGCAAAAAGGTTtcagt                 >  1:306961/1‑148 (MQ=255)                                                                                                                              aTTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagt                 <  8:296542/148‑1 (MQ=255)                                                                                                                               ttCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCagag                                                                       >  6:20589/1‑93 (MQ=255)                                                                                                                               ttCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCagag                                                                       <  5:20589/93‑1 (MQ=255)                                                                                                                               ttCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagtca               <  4:120833/149‑1 (MQ=255)                                                                                                                                 cGATTTGTTACGCTCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagtcag              <  2:364826/148‑1 (MQ=255)                                                                                                                                  gATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAgtg            <  8:308304/149‑1 (MQ=255)                                                                                                                                  gATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCGCATAGGGCAAAAACGTTTCAGTCAgtg            >  6:98689/1‑149 (MQ=255)                                                                                                                                  gATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGC‑AAAACGTTTCAGTCAgtgt           <  2:134299/149‑1 (MQ=255)                                                                                                                                       gTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGtaat        <  6:272484/148‑1 (MQ=255)                                                                                                                                           cgcgCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTGAGAGCAGGATGATTCATAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATaaaa    <  5:214360/148‑1 (MQ=255)                                                                                                                                            gcgcAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATaaaaaa  <  5:190717/149‑1 (MQ=255)                                                                                                                                            gcgcAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATaaaaa   >  4:352234/1‑148 (MQ=255)                                                                                                                                            gcgcAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATaaaaa   >  5:56237/1‑148 (MQ=255)                                                                                                                                               |                                                                                                                                                  GCTGGCAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAA  >  NC_000913/1934445‑1934732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑