Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,452,110 | T→C | 6.8% | K511K (AAA→AAG) | paaZ ← | oxepin‑CoA hydrolase and 3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,452,110 | 0 | T | C | 6.8% | 173.0 / 3.3 | 60 | K511K (AAA→AAG) | paaZ | oxepin‑CoA hydrolase and 3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase |
Reads supporting (aligned to +/- strand): ref base T (28/26); new base C (2/2); total (31/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01 |
CGCTGAGGCAAGCAAAGTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCA > NC_000913/1451965‑1452254 | cgcTGAGGCAAGCAAAGTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTa < 1:337923/149‑1 (MQ=255) gcTGAGGCAAGCAAAGTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTAc < 5:359688/149‑1 (MQ=255) gcTGAGGCAAGCAAAGTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTAc < 6:359143/149‑1 (MQ=255) ggCAAGCAAAGTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATa < 2:393353/149‑1 (MQ=255) aagcaaAGTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGCCTTTCGCACCGCGCACCCACTGTTTACTGATAGCg > 5:256660/1‑149 (MQ=255) aaCAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCGTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGGACCCACGGGTTACTGATAGCGGCAAGCGtcg > 8:217862/1‑149 (MQ=255) tatCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGa < 7:279744/149‑1 (MQ=255) tatCGGCCTCTGTCATTGTGCGGCGGGCAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCACCAAATATTTCAGGAACGGATGCATACTATCTGCTTCGACATTCGCCCCGCGAAACCACTGTTTACTGATAGCGCCAAGCATCGTCGGa > 4:22584/1‑149 (MQ=255) tCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGAc < 7:245746/148‑1 (MQ=255) tCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATATTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGAGGCAAGCATCGTCGGAc > 6:310536/1‑148 (MQ=255) gCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAAAACGATCTTCTTAGACTTTCGCACCGCGCACCCACGGTTTACTGATAGCGCCAAGCATCGTCGGACTAcc > 5:10483/1‑149 (MQ=255) ccTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGc < 3:50994/130‑1 (MQ=255) ccTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGAGCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGc > 4:50994/1‑130 (MQ=255) tGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGaa > 4:78083/1‑149 (MQ=255) gTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGaa > 5:156291/1‑148 (MQ=255) gTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAAc > 1:229070/1‑149 (MQ=255) ttGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCg > 6:104309/1‑149 (MQ=255) gcggGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGCCTACCCTGAACAGCGGTTCGCt > 2:192795/1‑149 (MQ=255) ggAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCAt < 7:99969/149‑1 (MQ=255) aGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCTGCAAGCATCGTCGGACTACACTGAACAGCGGGTCTCTGCATg > 4:293235/1‑148 (MQ=255) gTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGt > 7:121612/1‑148 (MQ=255) aaCAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGTACTACCCTGAACAGCGGTTCGCTGCATGTAATg > 2:252185/1‑149 (MQ=255) aCAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGCCTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGt > 4:330325/1‑149 (MQ=255) cAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGtt < 7:217862/149‑1 (MQ=255) cAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGtt > 3:136058/1‑149 (MQ=255) gCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCa < 4:305528/149‑1 (MQ=255) cTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCg > 7:177614/1‑148 (MQ=255) tGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCCAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGt > 4:16356/1‑148 (MQ=255) tGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGCTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGt > 6:180998/1‑148 (MQ=255) tGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGCTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGt > 2:22772/1‑148 (MQ=255) gTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACGCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTaaa > 4:297939/1‑149 (MQ=255) gTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTaaa > 8:42545/1‑149 (MQ=255) gTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCCCCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCg > 3:230385/1‑149 (MQ=255) tcctcAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTaa > 6:108440/1‑149 (MQ=255) tcAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCCCTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTGCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAAttc > 3:257825/1‑149 (MQ=255) aaaaTATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAAttctt > 8:227451/1‑149 (MQ=255) aaaaTATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGCTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAAttc < 5:180998/147‑1 (MQ=255) aaaaTATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGCTTACTGATACCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAAttc < 1:22772/147‑1 (MQ=255) aaTATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCAt < 4:277532/112‑1 (MQ=255) aaTATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCAt > 3:277532/1‑112 (MQ=255) atatTTGCGGAACTGATGAATAGGATCTTCTTCGACTTTCGCACCTCGCACCCAGTGTTTACTGATAGCGGCAAGCATCGTAGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCTCCTAATTCTTCAc < 2:183333/149‑1 (MQ=255) aaCGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTgc < 8:177614/149‑1 (MQ=255) aTGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGAc < 7:227451/149‑1 (MQ=255) aTGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTAGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGAc < 6:156291/149‑1 (MQ=255) aaTACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCa < 4:151212/148‑1 (MQ=255) aaTACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAg < 5:104309/149‑1 (MQ=255) aTACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGAcc < 3:196794/146‑1 (MQ=255) aTACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGAcc > 4:196794/1‑146 (MQ=255) aTACGATCTTCTTCGACTTTCGCACCGCGCACACACTGTTTACTTATAGCGGCAATCATCTTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTATACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAg < 2:31868/148‑1 (MQ=255) gATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGATGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGccaa > 4:162866/1‑147 (MQ=255) ttcGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTa > 5:382406/1‑149 (MQ=255) ttcGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTa > 2:287448/1‑149 (MQ=255) cGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGt < 1:252185/146‑1 (MQ=255) gACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCACGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCa < 5:276595/149‑1 (MQ=255) cGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTtgt < 3:78083/148‑1 (MQ=255) gcgcACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTg < 8:121612/149‑1 (MQ=255) gcACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTggg < 7:42545/149‑1 (MQ=255) ccccACTGGTTACTGATAGCGGCAAGCATCGGCGGACTACCCTGAACAGCGGGTCGCTGCATGTAATGTTGCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGa < 1:192795/148‑1 (MQ=255) aCCCCCTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGa < 3:293235/149‑1 (MQ=255) aCTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCa < 3:297939/149‑1 (MQ=255) | CGCTGAGGCAAGCAAAGTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCA > NC_000913/1451965‑1452254 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |