Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,087,476 C→T 7.4% R124H (CGC→CAC)  pgaC ← biofilm PGA synthase PgaCD, catalytic subunit; poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,087,4760CT 7.4% 158.2 / 3.0 54R124H (CGC→CAC) pgaCbiofilm PGA synthase PgaCD, catalytic subunit; poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
Reads supporting (aligned to +/- strand):  ref base C (21/29);  new base T (2/2);  total (23/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01

GCCGCATCGCGGTCTAATAACGCATCGCCATCAATGCACACCAGATATTCACTTTTCGCCGCGGCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGAC  >  NC_000913/1087329‑1087622
                                                                                                                                                   |                                                                                                                                                  
gccgcATCGCGGTCTAATAACGCATCGCCATCAATGCACACCAGATATTCACTTTTCGCCGCGGCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGc                                                                                                                                                    >  6:356741/1‑148 (MQ=255)
              taataaCGCATCGCCATCAATGCACACCAGATATTCACTTTTCGCCGCGGCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATTCGATCCAGGATGGCAc                                                                                                                                     >  8:217825/1‑149 (MQ=255)
                ataaCGCATCGCCATCAATGCACACCAGATATTCACTTTTCGCCGCGGCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACgg                                                                                                                                   >  4:138482/1‑149 (MQ=255)
                   aCGCATCGCCATCAATGCACACCAGATATTCACTTTTCGCCGCGGCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGtt                                                                                                                                >  1:163959/1‑149 (MQ=255)
                           ccATCAATGCACACCAGATATTCACTTTTCGCCGCGGCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGt                                                                                                                        <  8:305538/149‑1 (MQ=255)
                           ccATCAATGCACACCAGATATTCACTTTTCGCCGCGGCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGt                                                                                                                        <  4:33408/149‑1 (MQ=255)
                                         cAGATATTCACTTTTCGCCGCGGCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTa                                                                                                          <  3:221639/149‑1 (MQ=255)
                                           gATATTCACTTTTCGCCGCGGCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACg                                                                                                        <  3:213377/149‑1 (MQ=255)
                                           gATATTCACTTTTCGCCGCGGCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCACATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACg                                                                                                        <  5:331131/149‑1 (MQ=255)
                                            atatTCACTTTTCGCCGCGGCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATTACCCGCAAATGGGGAATTTGTGCAGCCATGCTCTCAAGGATGGCACGGGATTTCTCTGTTGACCCGTCATTTCCgg                                                                                                       >  6:223251/1‑149 (MQ=255)
                                              atTCACTTTTCGCCGCGGCAGCTCCGCTTTTAAGCGCAAAGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGAAGGCACGGGTTATATCTGTTGAACCGTCATTTACGGc                                                                                                      >  4:370178/1‑148 (MQ=255)
                                               ttCACTTTTCGCCGCGGCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCaa                                                                                                    >  4:243865/1‑149 (MQ=255)
                                                 cACTTTTCGCCGCGGCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGATTTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAAt                                                                                                   >  5:272680/1‑148 (MQ=255)
                                                  aCTTTTCGCCGCGGCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATaa                                                                                                 >  6:194532/1‑149 (MQ=255)
                                                  aCTTTTCGCCGCGGCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATaa                                                                                                 <  4:268123/149‑1 (MQ=255)
                                                      ttCGCCGCGGCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACACGCTAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGTTTTTATCTGTTGAACCGTCATTTACGGCAATAACtt                                                                                              <  1:363303/148‑1 (MQ=255)
                                                         gccgcGGCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAAt                                                                                          >  8:135221/1‑149 (MQ=255)
                                                           cgcgGCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATcc                                                                                                                                               >  4:361449/1‑94 (MQ=255)
                                                           cgcgGCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATcc                                                                                                                                               <  3:361449/94‑1 (MQ=255)
                                                              ggCAGCTCCGGTTTTAAGCGCAATGGCTTTCCACTGGTGTTGCGCCAGATGAATGACCCGCAAATGGGTAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTtct                                                                                     <  1:350669/149‑1 (MQ=255)
                                                               gCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTtctc                                                                                    <  7:258467/149‑1 (MQ=255)
                                                               gCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTtctc                                                                                    <  7:264606/149‑1 (MQ=255)
                                                               gCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATCAATGACCCGCAAATGGGGAATTTGTGCAGCCATGTGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTtct                                                                                     >  4:76885/1‑148 (MQ=255)
                                                                  gCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCAt                                                                                  >  8:186628/1‑148 (MQ=255)
                                                                   cTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGAGCCGCAAATGGGGAATTTGTGCAGCCATGCGATCGAGGATGGCTCGGGTTTTATCTGTTGAACCGTCATTTTCGGCAATAACTTCAATGTTCTCATaa                                                                                <  3:370178/149‑1 (MQ=255)
                                                                    tCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGTGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAAc                                                                               >  6:191700/1‑149 (MQ=255)
                                                                     ccGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACg                                                                              >  6:66628/1‑149 (MQ=255)
                                                                        gTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCtg                                                                           <  3:66819/149‑1 (MQ=255)
                                                                            tAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGTGGAATTTGTGCAGCAATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGc                                                                        <  5:223251/148‑1 (MQ=255)
                                                                            tAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGc                                                                        <  7:119655/148‑1 (MQ=255)
                                                                                    aTGGCTTTCCCCTGGTTTTGCTCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGCTAAAgcggc                                                               <  3:128359/149‑1 (MQ=255)
                                                                                    aTGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGCTAAAgcggc                                                               <  2:402007/149‑1 (MQ=255)
                                                                                       gCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTAt                                                                                                                            >  4:222008/1‑85 (MQ=255)
                                                                                       gCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTAt                                                                                                                            <  3:222008/85‑1 (MQ=255)
                                                                                            ccccTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATg                                                        <  3:243865/148‑1 (MQ=255)
                                                                                             cccTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGCTaaa                                                                    >  4:313168/1‑135 (MQ=255)
                                                                                             cccTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGCTaaa                                                                    <  3:313168/135‑1 (MQ=255)
                                                                                              ccTGGTTTTGCGCCAGATGAATGACCCGCAAATGTGGAATTTGTGCAGCCATGCGATCAAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAAAAACTTCAATGTTCTCATAACGCTGAGCTAAAGCGGCGTGTATGGtt                                                     >  3:103461/1‑149 (MQ=255)
                                                                                              ccTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTtct                                                                                     >  1:189710/1‑117 (MQ=255)
                                                                                              ccTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTtct                                                                                     <  2:189710/117‑1 (MQ=255)
                                                                                                  gTTTTGCGCCAGATGACTGACCCGCACAAGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGATGAACCGGCATTTACGGCAATAACTTCACTGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATGGTTTc                                                   <  2:262814/147‑1 (MQ=255)
                                                                                                  gTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATGGTTTc                                                   <  1:77073/147‑1 (MQ=255)
                                                                                                    tttGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGTGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATg                                                        <  8:139011/140‑1 (MQ=255)
                                                                                                    tttGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGTGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATg                                                        >  7:139011/1‑140 (MQ=255)
                                                                                                          ccAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGttt                                         <  5:66628/149‑1 (MQ=255)
                                                                                                          ccAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGttt                                         >  5:278005/1‑149 (MQ=255)
                                                                                                                 aatgaCCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTctcc                                   <  7:135221/148‑1 (MQ=255)
                                                                                                                      cccGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTa                             <  2:163959/149‑1 (MQ=255)
                                                                                                                       ccGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTaa                            <  3:377125/149‑1 (MQ=255)
                                                                                                                               ggggAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAggg                     >  2:164972/1‑148 (MQ=255)
                                                                                                                               ggggAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAggg                     >  5:354258/1‑148 (MQ=255)
                                                                                                                                gggAATTTGTGCAGCCATGTGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGaa                   <  3:76885/149‑1 (MQ=255)
                                                                                                                                         tgCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATg           <  7:217825/148‑1 (MQ=255)
                                                                                                                                           cAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGga         <  8:358583/148‑1 (MQ=255)
                                                                                                                                                 tGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGAc  >  1:113252/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCCGCATCGCGGTCTAATAACGCATCGCCATCAATGCACACCAGATATTCACTTTTCGCCGCGGCAGCTCCGGTTTTAAGCGCAATGGCTTTCCCCTGGTTTTGCGCCAGATGAATGACCCGCAAATGGGGAATTTGTGCAGCCATGCGATCCAGGATGGCACGGGTTTTATCTGTTGAACCGTCATTTACGGCAATAACTTCAATGTTCTCATAACGCTGTGCTAAAGCGGCGTGTATGGTTTCCTCAACGTTTTTCTCCTCATTAAAACAGGGAATGATAATGGAGATAGAC  >  NC_000913/1087329‑1087622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑