Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,071,412 G→A 6.4% N118N (AAC→AAT)  rutD ← putative reactive intermediate detoxifying aminoacrylate hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,071,4120GA 6.4% 191.1 / 2.8 62N118N (AAC→AAT) rutDputative reactive intermediate detoxifying aminoacrylate hydrolase
Reads supporting (aligned to +/- strand):  ref base G (24/34);  new base A (2/2);  total (26/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

CCAGCGCGTCTTCTGCCTCCAGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGT  >  NC_000913/1071264‑1071559
                                                                                                                                                    |                                                                                                                                                   
ccAGCGCGTCTTCTGCCTACAGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGTGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCg                                                                                                                                                     >  7:9927/1‑149 (MQ=255)
 cAGCGCGTCTTCTGCCTCCTGGGGAGGTGCGGGGGCCGCCATCCAGTCGGCTTGATAGTGGAACAACGGCTGCGCTTCCACCCATGCCTGCGGGCCGCCGCTTTACAGTATTCGTTCGCGAACCTGAAAACAGCGTCGCGTATGGGCGt                                                                                                                                                    <  8:9927/149‑1 (MQ=255)
 cAGCGCGTCTTCTGCCTCCAGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGt                                                                                                                                                    >  8:384254/1‑149 (MQ=255)
 cAGCGCGTCTTCTGCCTCCAGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGt                                                                                                                                                    <  5:170156/149‑1 (MQ=255)
 cAGCGCGTCTTCTGCCTCCAGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTT‑CTCCGATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGtt                                                                                                                                                   <  6:151887/149‑1 (MQ=255)
         cttctGCCTCCAGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCg                                                                                                                                             >  6:181231/1‑148 (MQ=255)
         cttctGCCTCCAGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCg                                                                                                                                             >  5:213375/1‑148 (MQ=255)
              gCCTCCAGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAACACAGCGGCGCGTATGGGCGTTTATTCGTagc                                                                                                                                         >  5:253751/1‑147 (MQ=255)
               ccTCCAGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTagccag                                                                                                                                      >  8:359875/1‑149 (MQ=255)
               ccTCCAGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTagccag                                                                                                                                      >  2:12935/1‑149 (MQ=255)
                   cAGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAGAACAGCGGCGCGTATGGGCGTTTATTCGTagccagcc                                                                                                                                    <  8:74328/147‑1 (MQ=255)
                   cAGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCg                                                                                                                                   <  1:100317/148‑1 (MQ=255)
                   cAGGCGAGGGGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTagccagcc                                                                                                                                    <  5:43893/147‑1 (MQ=255)
                      gCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTaa                                                                                                                               >  4:180864/1‑149 (MQ=255)
                      gCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCATTaa                                                                                                                               <  6:213375/149‑1 (MQ=255)
                         aGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATAGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGc                                                                                                                            >  5:298019/1‑149 (MQ=255)
                              cgcgGGCCGCCATCCAGTCGGCGGTATAGAGGAACAACGGATGCGCTTCCACCCCTCACTGCGCGCCGCCGCTATAAAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGAt                                                                                                                        <  6:275280/148‑1 (MQ=255)
                                    ccgccATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGcaca                                                                                                                 <  6:299941/149‑1 (MQ=255)
                                    ccgccATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGcaca                                                                                                                 <  3:272158/149‑1 (MQ=255)
                                     cgccATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAg                                                                                                                <  1:177918/149‑1 (MQ=255)
                                      gccATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGt                                                                                                               <  6:244321/149‑1 (MQ=255)
                                       ccATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGt                                                                                                               >  5:35559/1‑148 (MQ=255)
                                            cAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGa                                                                                                         <  7:359875/149‑1 (MQ=255)
                                             aGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGcac                                                                                                                  <  7:122997/139‑1 (MQ=255)
                                             aGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGcac                                                                                                                  >  8:122997/1‑139 (MQ=255)
                                                  gcggGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGATCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGAcgcgc                                                                                                    >  4:52638/1‑147 (MQ=255)
                                                   cggGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGa                                                                                                  <  3:180864/149‑1 (MQ=255)
                                                     ggATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATa                                                                                                <  5:355736/149‑1 (MQ=255)
                                                        ttgagGAACAACGTATGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATAAAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGTATAATc                                                                                             <  6:127015/147‑1 (MQ=255)
                                                            ggAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAgc                                                                                         <  7:10090/149‑1 (MQ=255)
                                                            ggAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAgc                                                                                         <  6:253751/149‑1 (MQ=255)
                                                                 aacGGCTGCGCTTCCACCCATGCCTGCGCGCCCCCGCTATACAGTAATCGTTCGCGCACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAg                                                                                    >  3:312095/1‑149 (MQ=255)
                                                                       tGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCa                                                                               >  8:176677/1‑148 (MQ=255)
                                                                          gcTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTcc                                                                           >  4:174218/1‑149 (MQ=255)
                                                                              cccccgaTGCCTGCGCGCCGCCGCTATACAGTATGCGTTCGCGAACCTGAAAACACCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCCGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCccac                                                                        <  4:339814/142‑1 (MQ=255)
                                                                              ccACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCccac                                                                        <  1:12935/148‑1 (MQ=255)
                                                                                  ccATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAgcg                                                                   >  3:318269/1‑149 (MQ=255)
                                                                                       ccTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCCGCTGCATTCCCACCAGCGCACCg                                                              >  8:405080/1‑149 (MQ=255)
                                                                                         tGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCATCAGCGCACCGAg                                                            >  4:123410/1‑149 (MQ=255)
                                                                                                         aCAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATgg                                                     <  6:184401/140‑1 (MQ=255)
                                                                                                         aCAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATgg                                                     >  5:184401/1‑140 (MQ=255)
                                                                                                           aGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGc                                          <  5:181231/149‑1 (MQ=255)
                                                                                                           aGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGc                                          <  6:35559/149‑1 (MQ=255)
                                                                                                                  gTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATg                                    <  4:336691/148‑1 (MQ=255)
                                                                                                                  gTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGc                                   >  2:287266/1‑149 (MQ=255)
                                                                                                                   ttCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGCGCGCATGGCCGCCCACTGCGTAATGCt                                  >  8:312227/1‑149 (MQ=255)
                                                                                                                   atCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCt                                  <  8:292348/148‑1 (MQ=255)
                                                                                                                    tCGCGAACCTGAAAACAGCGGCGCGTATGGGCATTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCt                                  >  7:262476/1‑148 (MQ=255)
                                                                                                                    tCGCGAACCTGAAAACAGCGGCGCGTATGGGCATTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCt                                  >  7:262486/1‑148 (MQ=255)
                                                                                                                     cgcgAACCTGAAAACAGCGGCGCGTATGGGCATTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTc                                 <  8:262486/148‑1 (MQ=255)
                                                                                                                     cgcgAACCTGAAAACAGCGGCGCGTATGGGCATTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTc                                 <  8:262476/148‑1 (MQ=255)
                                                                                                                      gcgAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCaa                               >  7:171765/1‑149 (MQ=255)
                                                                                                                            cTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGTGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTg                         <  6:298019/149‑1 (MQ=255)
                                                                                                                             tGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCcca                                                                         <  2:240114/100‑1 (MQ=255)
                                                                                                                             tGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCcca                                                                         >  1:240114/1‑100 (MQ=255)
                                                                                                                             tGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGc                        >  3:13488/1‑149 (MQ=255)
                                                                                                                               aaaaCAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGc                        <  4:173342/147‑1 (MQ=255)
                                                                                                                                aaaCAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGc                     <  7:176677/149‑1 (MQ=255)
                                                                                                                                      cggcgCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAg               >  6:176120/1‑149 (MQ=255)
                                                                                                                                               tGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGAt       <  2:88146/148‑1 (MQ=255)
                                                                                                                                                 ggCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCa    <  3:174218/149‑1 (MQ=255)
                                                                                                                                                  gCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAg   <  4:318269/149‑1 (MQ=255)
                                                                                                                                                   cGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGt  <  1:287266/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCAGCGCGTCTTCTGCCTCCAGGCGAGGTGCGCGGGCCGCCATCCAGTCGGCGGGATAGAGGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGT  >  NC_000913/1071264‑1071559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑