Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,934,582	A→G	V8A (GTA→GCA)	edd ←	6‑phosphogluconate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,934,582	0	A	G	100.0%	113.9 / NA	33	V8A (GTA→GCA)	edd	6‑phosphogluconate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/15);  total (18/15)

GAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAG  >  NC_000913/1934458‑1934720                                                                                                                             |                                                                                                                                           gAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCAt                                                                                                                      >  3:151741/1‑147 (MQ=255)    aCCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAgg                                                                                                                 <  6:71832/149‑1 (MQ=255)                                   gAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTc                                                                                              <  1:149995/137‑1 (MQ=255)                                   gAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTc                                                                                              >  2:149995/1‑137 (MQ=255)                                         tGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGa                                                                                                    <  3:74687/125‑1 (MQ=255)                                         tGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGa                                                                                                    >  4:74687/1‑125 (MQ=255)                                         tGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTcac                                                                             >  7:148988/1‑148 (MQ=255)                                                tCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGtt                                                                     >  6:61367/1‑149 (MQ=255)                                                      ttttCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGa                                                                                                    <  4:8883/112‑1 (MQ=255)                                                      ttttCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGa                                                                                                    >  3:8883/1‑112 (MQ=255)                                                          cGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCaga                                                            <  5:61367/148‑1 (MQ=255)                                                            cTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGa                                                         >  2:143356/1‑149 (MQ=255)                                                            cTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGa                                                         >  2:146054/1‑149 (MQ=255)                                                                        gggCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGa                                              >  8:121333/1‑148 (MQ=255)                                                                          gCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAAc                                           >  8:84887/1‑149 (MQ=255)                                                                               aTAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTg                                      >  8:63162/1‑149 (MQ=255)                                                                                     agagCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCg                                <  8:148988/149‑1 (MQ=255)                                                                                            gTCTCGCGCGAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCt                           >  5:98502/1‑147 (MQ=255)                                                                                                 gcgcgAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATa                       <  1:146054/146‑1 (MQ=255)                                                                                                 gcgcgAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATa                       <  1:143356/146‑1 (MQ=255)                                                                                                 gcgcgAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAgg                     <  7:84887/148‑1 (MQ=255)                                                                                                 gcgcgAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAg                      <  6:98502/147‑1 (MQ=255)                                                                                                   gcgAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGAt                                                        <  2:132303/111‑1 (MQ=255)                                                                                                   gcgAACGTTCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGAt                                                        >  1:132303/1‑111 (MQ=255)                                                                                                           tCAATGATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGtt          >  2:16107/1‑149 (MQ=255)                                                                                                                gATTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagt     >  7:86373/1‑149 (MQ=255)                                                                                                                 aTTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTAt                                                    >  1:10919/1‑101 (MQ=255)                                                                                                                 aTTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTAt                                                    <  2:10919/101‑1 (MQ=255)                                                                                                                 aTTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACg            >  7:149020/1‑141 (MQ=255)                                                                                                                 aTTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACg            <  8:147052/141‑1 (MQ=255)                                                                                                                 aTTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACg            >  7:147052/1‑141 (MQ=255)                                                                                                                 aTTCGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACg            <  8:149020/141‑1 (MQ=255)                                                                                                                    cGATTTGTTGCGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCGGTAACCGGAGCTCATAGGGCAAAAACGTTtcagtcag  <  7:121333/148‑1 (MQ=255)                                                                                                                             |                                                                                                                                           GAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAG  >  NC_000913/1934458‑1934720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑