Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,560,976 G→T 19.5% intergenic (+78/‑392) yffL → / → yffM CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,560,9760GT19.5% 117.6 / 3.9 66intergenic (+78/‑392)yffL/yffMCPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (24/25);  new base T (7/3);  total (38/28)
Fisher's exact test for biased strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATACCAGGATCGGCAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTAGA  >  NC_000913/2560829‑2561120
                                                                                                                                                   |                                                                                                                                                 
tataCCAGGATCGGCAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAgt                                                                                                                                                  >  2:177821/1‑149 (MQ=255)
    ccAGGATCGGCAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAgtggt                                                                                                                                               <  6:6834/148‑1 (MQ=255)
    ccAGGATCGGCAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAgtggt                                                                                                                                               <  5:208409/148‑1 (MQ=255)
     cAGGATCGGCAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTTGtggtgg                                                                                                                                             >  3:316057/1‑149 (MQ=255)
     cAGGATCGGCAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAgtg                                                                                                                                                 <  6:25120/145‑1 (MQ=255)
     cAGGATCGGCAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAgtg                                                                                                                                                 >  5:25120/1‑145 (MQ=255)
     cAGGATCGGCAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGtggtgg                                                                                                                                             >  7:76655/1‑149 (MQ=255)
        gATCGGCAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTc                                                                                                                                          <  8:191677/149‑1 (MQ=255)
            ggCAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATTAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCaaaa                                                                                                                                      <  3:5433/149‑1 (MQ=255)
             gCAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAgtg                                                                                                                                                 >  8:93477/1‑137 (MQ=255)
             gCAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAgtg                                                                                                                                                 <  7:93477/137‑1 (MQ=255)
             gCAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTCGGTCAAAAGTTGCCGTTACTGGTGGGTAAAAAg                                                                                                                                     >  1:257371/1‑149 (MQ=255)
              cAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGt                                                                                                                                    >  8:251890/1‑149 (MQ=255)
              cAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGtt                                                                                                                                   >  7:326029/1‑149 (MQ=255)
              cAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGtt                                                                                                                                   >  7:331972/1‑149 (MQ=255)
                 aaaGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGc                                                                                                                                 <  7:212818/149‑1 (MQ=255)
                      gAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTa                                                                                                                            <  6:230846/149‑1 (MQ=255)
                       aGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTaa                                                                                                                           <  1:177821/149‑1 (MQ=255)
                       aGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGAGCTGGGGCAAAGGTTGCGGtg                                                                                                                             >  5:381443/1‑146 (MQ=255)
                         tGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGTTGGGTAAAAAGTTGACTTTAAgg                                                                                                                         >  1:188193/1‑149 (MQ=255)
                         tGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAgg                                                                                                                         >  8:221023/1‑149 (MQ=255)
                           cAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGCGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGATATTGATGTGTAAAAATATGCAGTTAAgt                                                                                                                         >  2:272947/1‑146 (MQ=255)
                            aaCGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAgtggtg                                                                                                                                              >  2:430705/1‑125 (MQ=255)
                            aaCGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAgtggtg                                                                                                                                              <  1:430705/125‑1 (MQ=255)
                            aaCGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTgg                                                                                                                      <  6:411401/149‑1 (MQ=255)
                            aaCGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTgg                                                                                                                      >  5:411401/1‑149 (MQ=255)
                            aaCGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGCTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGCTTCAAAAGTTGACGTTAAGGTg                                                                                                                       >  6:116253/1‑148 (MQ=255)
                              cGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTATTGGTGGGTCAAAAGATGCCTTTAAggagtg                                                                                                                     >  8:85253/1‑144 (MQ=255)
                                 aTACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAATTTGCGGTGTAGTTGGGTCa                                                                                                                  >  7:204607/1‑148 (MQ=255)
                                  tACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTTATGTTGGGTCAAATTTTGCCGTTAAGGTGGGGCaaa                                                                                                                >  5:20800/1‑149 (MQ=255)
                                    ccAGTTATTATTCAATCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATTAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCTGTTAAGGTGGGTCaaaa                                                                                                               <  3:344547/148‑1 (MQ=255)
                                    ccAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAg                                                                                                             >  3:225628/1‑149 (MQ=255)
                                     cAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTATTGGTGGGTCAAAAGTTGCCGTTATGGTGGGTCAAAAGt                                                                                                            >  5:238110/1‑149 (MQ=255)
                                     cAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGTTGTGTAAAAATTTGACGTTAAGTTGGCTAAAATGt                                                                                                            >  5:25858/1‑149 (MQ=255)
                                       gTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTg                                                                                                          <  7:221023/149‑1 (MQ=255)
                                          attattCAATCCGTACAACATGAAATAACGGAATCCGTCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGcc                                                                                                        <  4:225628/148‑1 (MQ=255)
                                          attattCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGTTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCg                                                                                                       >  5:52014/1‑149 (MQ=255)
                                             attCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTgg                                                                                                                      >  8:240296/1‑132 (MQ=255)
                                             attCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTgg                                                                                                                      <  7:240296/132‑1 (MQ=255)
                                             aatCAAGATGTACATCTTGAAATAAAGGACTCTGTTCGGCAATGTTAAGTCATTTTCTTTTCCTTTATTCCATGTATTCAGGTGGGTAAAAAGTTGGCGTTATTGTTGGGTAAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGtt                                                                                                     <  6:52014/146‑1 (MQ=12)
                                              ttCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGACGtt                                                                                                     >  7:312791/1‑147 (MQ=255)
                                               tCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTTATGATGGGTAAAAATTTGAGGTTAAGTTAGGTACTAAGTTGccaatatt                                                                                                  >  6:286746/1‑143 (MQ=255)
                                                 aaGCCGTACAACATGAAATAACGGAATCAGCCGTGCAATGCTCACGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGGCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAgg                                                                                                 <  5:116253/148‑1 (MQ=255)
                                                       tACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTTGTGGTGGGTAAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGACGTTAAGGTgtggc                                                                                           >  3:122355/1‑144 (MQ=255)
                                                         caacaTGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTATTGGTGGGTCAAAAGTTGCAGTTTAGGATGGTCTAAAGTTGCAGTGAATGTCGGTaaa                                                                                         >  3:244658/1‑148 (MQ=255)
                                                            caTGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTATTGTTGGGTAAAAAGTTGCCGTTAAGGTGTGTCAAAATTTTAAGTAAATGAGGGTCaaaatt                                                                                     >  7:143089/1‑147 (MQ=255)
                                                               gAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGGGGGTAAAAAGTTGCCGTTAAGGGGGGGGAAAAGTTGCAGTATAGGTGGGGCTAAATTTga                                                                                  >  6:194906/1‑148 (MQ=255)
                                                               gAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGATAATGGTGGGTAAAAATATGCCGTTAAGGTGGGGCAAATTTTGAAGTTAACGTGTGAGAAAAGTTg                                                                                   >  1:164437/1‑148 (MQ=255)
                                                                  aTAACGGAATCCGTCGGGCATTTATCCAGCCTTTTGTATGCCATAAATAAATGAATTCAGGGGGGAAAAAATATGCCGTTAGTGGTGGGTCAAAAGTTGCCTTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGa                                                                               <  8:76655/149‑1 (MQ=18)
                                                                          atacgcagggCATTTTTGCAGCCTTTTCTATCCCATTATTAAATGAATTCAGGTGGGTTAAAAGTTGCCGTTAGTGGTGGGTCATTAGATGCCTTTTAGGTGGGTCAAAAGTTTCCTTTAAGGTGGGTCAAAAGTTGCCGAGTAAGaa                                                                        <  4:218419/141‑1 (MQ=19)
                                                                          aTCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAAt                                                                       <  7:107424/149‑1 (MQ=255)
                                                                                  ggCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCaaaa                                                                                                               <  1:340850/102‑1 (MQ=255)
                                                                                  ggCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCaaaa                                                                                                               >  2:340850/1‑102 (MQ=255)
                                                                                   gCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTAAAATTTTGCCGTTTAGGTGGGTCAAAATTTGGAGTTAAGGTGTGTGTAATTTTGACGAGTAAGAATTCTACAAt                                                               >  7:429969/1‑148 (MQ=25)
                                                                                     aaTGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGATGCCGTTAGTGGGTGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTaa                                                            <  7:197620/149‑1 (MQ=255)
                                                                                       tGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTATTGGTGGGTCAAAATTTGCCTTTAAGGTGGGTCAAAAGTTGAAGTTAAGGTGTGGCAAAATTTTACTAGTAAGAATTCTAATATTatt                                                           >  8:315579/1‑146 (MQ=25)
                                                                                       tGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCaaaa                                                                                                               <  2:369083/97‑1 (MQ=255)
                                                                                       tGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCaaaa                                                                                                               >  1:369083/1‑97 (MQ=255)
                                                                                            cagCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGTGTAAAAAGTTGCCGTTAGTGGTGGGTAAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCt                                                     <  3:47580/147‑1 (MQ=255)
                                                                                              gCCTTTTCATTGCCTAAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTgg                                                                                              <  3:218257/106‑1 (MQ=255)
                                                                                                      tttGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAATGTTGGGTCAAAATTTGCCGTTAAGGAGTGTCAAATTTTGAAGTAAACGTGTCTCAAAATTTGACAACTAAAAATACTAGTATTGAGAATACTAGGctagtg                                            >  8:109374/1‑143 (MQ=16)
                                                                                                            ttAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTTTTGGTGGGTAAAAATTTGCAGTTAAGGGGTGTGAAAATTTTGAGGTTAAGGGGGGGAAAATTTTAGGAGTAAAGATTGTAAAATTAAGAATTAAAGGGGATGGGAGttta                                     >  2:201306/1‑148 (MQ=255)
                                                                                                               aattcATGAATTCAGGTGGGCCCAAATTTGCCGTTATTGGTGGCTAAAAAGTTGCCTTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTa                                  <  8:312791/145‑1 (MQ=37)
                                                                                                                 tCCAGGAATTCCGGGGGGCCCAAAGAAGCCCTTAGGGGTGGGTCAAAAGCGGCCGTTCCGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGc                                <  7:251890/149‑1 (MQ=25)
                                                                                                                       aaTTCAGGTGGCTAAAAATTTGGGTTTCTGGGTGGGTCAAATCTTGCCGTTAAGGTGGGTCAAATGTTTCATTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATAAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTccc                           <  8:317555/148‑1 (MQ=25)
                                                                                                                                  gTCAAAAGATGCCCGTAGTGGTGCCTCCACAGCAGCCGTGACGGGGTCCCAAATCTGCCCTTCAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATaga              <  7:23711/149‑1 (MQ=255)
                                                                                                                                   tCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTTAGGGGGGTCAAAAGTTTGCGTGAAGGTGGGGCAAAAGTTGCCTAGGAAGGAGTCTACAATTAATAATTATACGCGACGTGAGTGTTTTTGCGTAAcggtaggtgaaa                >  8:428735/1‑143 (MQ=16)
                                                                                                                                   tCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTTGGGAAAAATTTGCCGTTAAGGTGGGTGAAATGTTTGTGAGTAAGATTTGTACAATTAAGAATACTAGGCGACTGGAGTTTTGTAGCCTAGCGGTCGGTCa                  >  2:161769/1‑145 (MQ=25)
                                                                                                                                   tCAAAAGCTGCCGTTAGTGGTGGGTCAAAAGGAGCCGTTAAGGTGGGTCAAAAGTTGCCCTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATaga              <  7:85253/149‑1 (MQ=255)
                                                                                                                                   gcAAAAGTTGCCGTTAGTGTTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGATGGGAGTTTTTTAGCCTCCCGGTCGGTCATaga              <  7:109374/148‑1 (MQ=255)
                                                                                                                                              ccttAGTGGTGGGTCAAAAGTTGCCGTAAAGGTGGGTCAAAAGGTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATagaga            <  7:344057/138‑1 (MQ=255)
                                                                                                                                              cGTTAGTGGTGGGTCAAAAGTTGCCGTTTAGGTGGGTAAAAAGTTGCAGTTAAAGTGGGTCGAAAGTTGGAGAGTAAGCATTCTTCAATTTAGAATTCTAGGATACGGGACTTTTTTTGCCTCCCGGTCGGTAATagaga            >  8:344057/1‑140 (MQ=25)
                                                                                                                                              cGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGcc      >  7:406915/1‑146 (MQ=255)
                                                                                                                                               gTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTa    >  4:219791/1‑147 (MQ=255)
                                                                                                                                               gTTAGGGTTGGTTCAAACCCTGCCGTTAAGTTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTaga  <  7:18984/149‑1 (MQ=38)
                                                                                                                                               cttcgtggtgGGTAAATAGTTGCGGTTAAGGTGGGTCAAAAGTTGCCGTTCAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTaga  <  3:48564/145‑1 (MQ=38)
                                                                                                                                                   |                                                                                                                                                 
TATACCAGGATCGGCAGAAAGGGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTAGA  >  NC_000913/2560829‑2561120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: