Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,808,846 C→A 5.2% G130W (GGG→TGG)  yniB ← putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,808,8460CA 5.2% 266.1 / 3.5 77G130W (GGG→TGG) yniBputative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (34/39);  new base A (2/2);  total (36/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.82e-01

TAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCAT  >  NC_000913/1808698‑1808992
                                                                                                                                                    |                                                                                                                                                  
taaataaaCCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTcccc                                                                                                                                                    >  8:319249/1‑149 (MQ=255)
   ataaaCCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCttt                                                                                                                                                 <  1:302553/149‑1 (MQ=255)
    taaaCCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTg                                                                                                                                                >  7:327402/1‑149 (MQ=255)
     aaaCCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTgg                                                                                                                                               >  8:335028/1‑149 (MQ=255)
       aCCCAAGCAGAGAAAATAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCATGAAAATAGTATGGTGCGGACCAACAATACTGGACTCAAATTGTTCACGCGTTAGACCCTCTTCCCCTTTGGCt                                                                                                                                             >  3:282711/1‑149 (MQ=255)
         ccAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCttt                                                                                                                                           >  1:75889/1‑149 (MQ=255)
              cAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCa                                                                                                                                      <  7:142307/149‑1 (MQ=255)
                  gaAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAATCTGCATGAAAATAGTATTGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAAt                                                                                                                                  <  5:163613/149‑1 (MQ=255)
                    aaaGAACACATAACCTGCAGCAATACATATTACCGGCTGGATATACAGTGAGAAAACCTGCAGGTATATAGTATGGTGCGGAACAACAATACGGGACTGAATTTGTTCACGGGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGa                                                                                                                                <  2:413133/149‑1 (MQ=255)
                    aaaGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGa                                                                                                                                <  8:327402/149‑1 (MQ=255)
                    aaaGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGa                                                                                                                                <  3:281526/149‑1 (MQ=255)
                        aaCACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGtt                                                                                                                             >  3:305599/1‑148 (MQ=255)
                           acaTAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATc                                                                                                                         >  3:149608/1‑149 (MQ=255)
                             aTAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCtt                                                                                                                       >  5:367399/1‑149 (MQ=255)
                              tAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTc                                                                                                                      <  5:322003/149‑1 (MQ=255)
                              tAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTc                                                                                                                      <  6:213291/149‑1 (MQ=255)
                              tAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTc                                                                                                                      <  4:330082/149‑1 (MQ=255)
                              tAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTc                                                                                                                      <  2:150925/149‑1 (MQ=255)
                               aaCCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCATTTGGCTTTTTCCAGAATGAGTTGATCTTc                                                                                                                      >  3:13956/1‑148 (MQ=255)
                               aaCCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCATTTGGCTTTTTCCAGAATGAGTTGATCTTc                                                                                                                      >  3:19528/1‑148 (MQ=255)
                                aCCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCaa                                                                                                                    <  4:299980/149‑1 (MQ=255)
                                         aaTACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTcccc                                                                                                                                                    <  5:229226/108‑1 (MQ=255)
                                         aaTACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTcccc                                                                                                                                                    >  6:229226/1‑108 (MQ=255)
                                           tACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCg                                                                                                          >  1:299616/1‑148 (MQ=255)
                                            aCAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCCCGCGTTAGACCCTCTTCCACTTTGGCTTTTTCCATAATGAGTTGATCTTCAACACCTTCCCGc                                                                                                         >  2:347150/1‑148 (MQ=255)
                                              aaaTAACCCGCAGGATATACAGTGAGAAAAACTGCATGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTAGTTCACGCGTTAGCCCCTCTTCCCCTTTGTCTTTTTCCAGTATGAGTTGATCTTCAACACCTTCCCGCaag                                                                                                      <  2:301480/149‑1 (MQ=255)
                                               aaTAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTCGCTAATTCCAGAATGAGTTGATATTCAACACATTCCCGCaag                                                                                                      >  7:308608/1‑148 (MQ=255)
                                               aaTAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCATTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaaga                                                                                                     <  4:13956/149‑1 (MQ=255)
                                               aaTAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCATTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaaga                                                                                                     <  4:19528/149‑1 (MQ=255)
                                                aTAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAACATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaagaa                                                                                                    <  6:174447/149‑1 (MQ=255)
                                                aTAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaag                                                                                                      >  8:55848/1‑147 (MQ=255)
                                                    ccGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcgc                                                                                                >  2:102727/1‑149 (MQ=255)
                                                       gCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGcc                                                                                             <  4:351761/149‑1 (MQ=255)
                                                        cAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACTACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCt                                                                                            <  2:75889/149‑1 (MQ=255)
                                                        cAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCt                                                                                            >  4:216501/1‑149 (MQ=255)
                                                        cAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATATGTTCACGCGTTAGCCACTCGTCCCCTTTGGCTTTTACCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGAGCCt                                                                                            >  5:167297/1‑149 (MQ=255)
                                                          ggATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTgg                                                                                          >  8:69502/1‑149 (MQ=255)
                                                             tataCAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAacac                                                                                                                 >  2:244685/1‑123 (MQ=255)
                                                             tataCAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGa                                                                                       <  7:319249/149‑1 (MQ=255)
                                                              ataCAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGAc                                                                                      >  5:152407/1‑149 (MQ=255)
                                                                   gTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATc                                                                                 >  4:113518/1‑149 (MQ=255)
                                                                   gTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATc                                                                                 <  1:102727/149‑1 (MQ=255)
                                                                        aaaaaCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCggg                                                                             >  4:370866/1‑148 (MQ=255)
                                                                        aaaaaCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCggg                                                                             >  4:13472/1‑148 (MQ=255)
                                                                         aaaaCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCa                                                                           <  8:155951/149‑1 (MQ=255)
                                                                            aCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGTACTCAATTTGTTCACTCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCACGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCg                                                                        <  1:347150/149‑1 (MQ=255)
                                                                                   gAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACATTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCt                                                                 >  7:262201/1‑149 (MQ=255)
                                                                                       aTAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCaa                                                             <  5:293295/149‑1 (MQ=255)
                                                                                        tAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAAc                                                            <  4:263114/149‑1 (MQ=255)
                                                                                           tATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGcc                                                         <  6:367399/149‑1 (MQ=255)
                                                                                               gTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCa                                                        >  5:12604/1‑146 (MQ=255)
                                                                                               gTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCa                                                        <  6:12604/146‑1 (MQ=255)
                                                                                                tGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTcc                                                    <  5:271060/149‑1 (MQ=255)
                                                                                                tGCGGAAAATCAATACGGGACTCAATTTGTTCACGCGTTAGACCCTATTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGATGCGCGCCTGGCGACTCATCCGTGCACCGGAATCCTGCAACGCCAGTcc                                                    <  8:308608/149‑1 (MQ=255)
                                                                                                          caaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACATTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAAt                                           >  2:218468/1‑148 (MQ=255)
                                                                                                            aTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAg                                        <  7:335028/149‑1 (MQ=255)
                                                                                                             tACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAg                                        >  6:110340/1‑148 (MQ=255)
                                                                                                                gggACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCg                                                                               >  1:253511/1‑106 (MQ=255)
                                                                                                                gggACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCg                                                                               <  2:253511/106‑1 (MQ=255)
                                                                                                                 ggACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGa                                   <  7:55848/149‑1 (MQ=255)
                                                                                                                    cTCAATTTGTTCACGCGTTAGGCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTTGCGACTCATCCGGGCACCTGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGaaaa                                <  8:206422/149‑1 (MQ=255)
                                                                                                                     tCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAAc                                                            <  2:11238/120‑1 (MQ=255)
                                                                                                                     tCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAAc                                                            >  1:11238/1‑120 (MQ=255)
                                                                                                                     tCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGa                                      <  8:410628/142‑1 (MQ=255)
                                                                                                                     tCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGa                                      >  7:410628/1‑142 (MQ=255)
                                                                                                                       aaTTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCa                             >  4:210920/1‑149 (MQ=255)
                                                                                                                       aaTTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCa                             >  7:352544/1‑149 (MQ=255)
                                                                                                                       aaTTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGAGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATc                              <  1:218468/148‑1 (MQ=255)
                                                                                                                         tttGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCAcc                           <  3:13472/149‑1 (MQ=255)
                                                                                                                            gTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAgg                                       >  7:151085/1‑134 (MQ=255)
                                                                                                                            gTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAgg                                       <  8:151085/134‑1 (MQ=255)
                                                                                                                               cACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCTACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGaa                      <  5:110340/148‑1 (MQ=255)
                                                                                                                               cACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGaa                      <  3:210920/148‑1 (MQ=255)
                                                                                                                                       aGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGtt             >  6:113955/1‑149 (MQ=255)
                                                                                                                                        gCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGtt             <  6:152407/148‑1 (MQ=255)
                                                                                                                                        gCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGtt             <  5:131615/148‑1 (MQ=255)
                                                                                                                                         ccccTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCa           <  5:113955/149‑1 (MQ=255)
                                                                                                                                            ctctTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGCCTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAATTCAGGATGAAAAACCCCCAGAACATAACGTTCACGc        >  3:66171/1‑149 (MQ=255)
                                                                                                                                            ctctTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGc        >  8:329075/1‑149 (MQ=255)
                                                                                                                                               ttCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccac     >  3:375070/1‑149 (MQ=255)
                                                                                                                                                 ccccTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacca   >  4:230851/1‑149 (MQ=255)
                                                                                                                                                  cccTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTcc                                                    >  1:219031/1‑99 (MQ=255)
                                                                                                                                                  cccTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTcc                                                    <  2:219031/99‑1 (MQ=255)
                                                                                                                                                   ccTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCAt  >  4:164761/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCAT  >  NC_000913/1808698‑1808992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑