Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,807,117	A→G	S250G (AGT→GGT)	pfkB →	6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,807,117	0	A	G	100.0%	141.4 / NA	40	S250G (AGT→GGT)	pfkB	6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/20);  total (20/20)

GATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCC  >  NC_000913/1806979‑1807257                                                                                                                                           |                                                                                                                                             gATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTgg                                                                                                                                    <  6:3719/149‑1 (MQ=255)                  gcAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATgg                                                                                                                   >  1:99567/1‑149 (MQ=255)                            gTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGTTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGa                                                                                                          <  2:99567/148‑1 (MQ=255)                            gTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGAt                                                                                                         <  1:3333/149‑1 (MQ=255)                             ttAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGAt                                                                                                         >  2:194550/1‑148 (MQ=255)                                      gCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGa                                                                                                 >  8:67635/1‑147 (MQ=255)                                      gCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGa                                                                                                 <  7:67635/147‑1 (MQ=255)                                           gCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGa                                                                                                          <  1:189125/133‑1 (MQ=255)                                           gCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGa                                                                                                          >  2:189125/1‑133 (MQ=255)                                             cAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTgg                                                                                           >  4:78068/1‑146 (MQ=255)                                                    gTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGc                                                                                 >  4:100940/1‑149 (MQ=255)                                                        tCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGa                                                                                                                           <  6:34165/103‑1 (MQ=255)                                                        tCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGa                                                                                                                           >  5:34165/1‑103 (MQ=255)                                                               ccTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCgg                                                                                                               >  6:23746/1‑108 (MQ=255)                                                               ccTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCgg                                                                                                               <  5:23746/108‑1 (MQ=255)                                                               ccTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGaa                                                                       <  3:100940/148‑1 (MQ=255)                                                                cTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATACAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGaa                                                                       >  5:134413/1‑147 (MQ=255)                                                                    gTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATg                                                                 >  5:3366/1‑149 (MQ=255)                                                                         caAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTg                                                                                                                               >  3:61488/1‑82 (MQ=255)                                                                         caAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTg                                                                                                                               <  4:61488/82‑1 (MQ=255)                                                                            ggAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGAt                                                                  <  8:59087/140‑1 (MQ=255)                                                                            ggAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGAt                                                                  >  7:59087/1‑140 (MQ=255)                                                                             gAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGa                                                                                                          <  4:3075/99‑1 (MQ=255)                                                                             gAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGa                                                                                                          >  3:3075/1‑99 (MQ=255)                                                                              aGCGCTGGGTGTTGATAGTGAAAACTGTATACAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTg                                                       <  6:134413/149‑1 (MQ=255)                                                                                     ggTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTa                                                  <  1:194550/147‑1 (MQ=255)                                                                                         ttGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCg                                            >  8:48343/1‑149 (MQ=255)                                                                                           gATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCggg                                          >  1:99173/1‑149 (MQ=255)                                                                                             tAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGa                                        <  2:99173/149‑1 (MQ=255)                                                                                                tGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTgg                                                                                           >  5:1260/1‑95 (MQ=255)                                                                                                tGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTgg                                                                                           <  6:1260/95‑1 (MQ=255)                                                                                                         tATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCacac                            <  3:78068/149‑1 (MQ=255)                                                                                                              aGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTa                                                  >  5:110935/1‑122 (MQ=255)                                                                                                              aGGTGGTGCCACCACCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTa                                                  <  6:110935/122‑1 (MQ=255)                                                                                                                       caccacCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgg                                           <  1:105900/120‑1 (MQ=255)                                                                                                                       caccacCGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgg                                           >  2:105900/1‑120 (MQ=255)                                                                                                                        accaccGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAacac             >  2:70608/1‑149 (MQ=255)                                                                                                                          caccGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAgaga                                                                   >  4:20667/1‑93 (MQ=255)                                                                                                                          caccGGTGAAAAGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAgaga                                                                   <  3:20667/93‑1 (MQ=255)                                                                                                                                   aaaGCCAGGGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTcc  <  7:48343/149‑1 (MQ=255)                                                                                                                                           |                                                                                                                                             GATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCC  >  NC_000913/1806979‑1807257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑